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248 results on '"Gengyo-Ando, Keiko"'

1. Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Author

Tominami, Kanako, primary, Kudo, Tada-aki, additional, Noguchi, Takuya, additional, Hayashi, Yohei, additional, Luo, You-Ran, additional, Tanaka, Takakuni, additional, Matsushita, Ayumu, additional, Izumi, Satoshi, additional, Sato, Hajime, additional, Gengyo-Ando, Keiko, additional, Matsuzawa, Atsushi, additional, Hong, Guang, additional, and Nakai, Junichi, additional

Published
2024
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6. The plexin PLX-2 and the ephrin EFN-4 have distinct roles in MAB-20/ semaphorin 2A signaling in Caenorhabditis elegans morphogenesis

Author

Nakao, Fumi, Hudson, Martin L., Suzuki, Motoshi, Peckler, Zachary, Kurokawa, Rie, Liu, Zhicen, Gengyo-Ando, Keiko, Nukazuka, Akira, Fujii, Takashi, Suto, Fumikazu, Shibata, Yukimasa, Shioi, Go, Fujisawa, Hajime, Mitani, Shohei, Chisholm, Andrew D., and Takagi, Shin

Subjects
Caenorhabditis elegans -- Genetic aspects, Morphogenesis -- Genetic aspects, Cellular signal transduction -- Evaluation, Biological sciences
Abstract

Semaphorins are extracellular proteins that regulate axon guidance and morphogenesis by interacting with a variety of cell surface receptors. Most semaphorins interact with plexin-containing receptor complexes, although some interact with non-plexin receptors. Class 2 semaphorins are secreted molecules that control axon guidance and epidermal morphogenesis in Drosophila and Caenorhabditis elegans. We show that the C. elegans class 2 semaphorin MAB-20 binds the plexin PLX-2. plx-2 mutations enhance the phenotypes of hypomorphic mab-20 alleles but not those of mab-20 null alleles, indicating that plx-2 and mab-20 act in a common pathway. Both mab-20 and plx-2 mutations affect epidermal morphogenesis during embryonic and in postembryonic development. In both contexts, plx-2 null mutant phenotypes are much less severe than mab-20 null phenotypes, indicating that PLX-2 is not essential for MAB-90 signaling. Mutations in the ephrin efn-4 do not synergize with mab-20, indicating that EFN-4 may act in MAB-90 signaling. EFN-4 and PLX-9 are coexpressed in the late embryonic epidermis where they play redundant roles in MAB-20-dependent cell sorting.

Published
2007

8. Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans

Author

Mizuguchi, Souhei, Uyama, Toru, Kitagawa, Hiroshi, Nomura, Kazuko H., Dejima, Katsufumi, Gengyo-Ando, Keiko, Mitani, Shohei, Sugahara, Kazuyuki, and Nomura, Kazuya

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Souhei Mizuguchi [1, 2]; Toru Uyama [3]; Hiroshi Kitagawa [3]; Kazuko H. Nomura [1, 2, 4]; Katsufumi Dejima [1, 2]; Keiko Gengyo-Ando [5]; Shohei Mitani [4, 5]; Kazuyuki Sugahara [...]

Published
2003
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9. HEN-1, a secretory protein with an LDL receptor motif, regulates sensory integration and learning in Caenorhabditis elegans

Author

Ishihara, Takeshi, Lino, Yuichi, Mohri, Akiko, Mori, Ikue, Gengyo-Ando, Keiko, Mitani, Shohei, and Katsura, Isao

Subjects
Cytochemistry -- Research, Caenorhabditis elegans -- Physiological aspects, Animal models in research -- Evaluation, Biological sciences
Abstract

HEN-1, a secretory protein with an LDL receptor motif, has been found to regulate sensory integration and learning in Caenorhabditis elegans. It appears that C. elegans can be an ideal model organism for studying informational processing.

Published
2002

19. Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method

Author

Kage-Nakadai Eriko, Kobuna Hiroyuki, Funatsu Osamu, Otori Muneyoshi, Gengyo-Ando Keiko, Yoshina Sawako, Hori Sayaka, and Mitani Shohei

Subjects
Biotechnology, TP248.13-248.65
Abstract

Abstract Background Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have been developed to create single- or low-copy chromosomal integrated lines. Here we report an alternative method using ultraviolet trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations. Results We successfully integrated low-copy transgenes from extrachromosomal arrays using positive selection based on temperature sensitivity with a vps-45 rescue fragment and negative selection based on benzimidazole sensitivity with a ben-1 rescue fragment. We confirmed that the integrants express transgenes in the germline. Quantitative PCR revealed that strains generated by this method contain single- or low-copy transgenes. Moreover, positive selection marker genes flanked by LoxP sites were excised by Cre recombinase mRNA microinjection, demonstrating Cre-mediated chromosomal excision for the first time in C. elegans. Conclusion Our UV/TMP integration method, based on familiar extrachromosomal transgenics, provides a useful approach for generating single/low-copy gene integrations.

Published
2012
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20. Additional file 1: of Role of tyramine in calcium dynamics of GABAergic neurons and escape behavior in Caenorhabditis elegans

Author

Kagawa-Nagamura, Yuko, Gengyo-Ando, Keiko, Ohkura, Masamichi, and Nakai, Junichi

Abstract

Figure S1. ICaST system for optogenetic control and simultaneous calcium imaging in freely moving animals. The light paths are indicated by colored arrows. Details are described in the Materials and Methods and a previous report [27]. Figure S2. R-CaMP2 imaging in RME. (A) Representative images of a transgenic animal expressing both R-CaMP2 and EGFP in RME neurons in forward (top panels) and backward (bottom panels) movements. Transmitted-light images (TD), raw fluorescent images of R-CaMP2 and EGFP, fluorescent merged images, and pseudocolor ratio images (R-CaMP2/EGFP) are shown. (B) Fluorescent intensity ratio values (R=R-CaMP2/EGFP) of RME in a freely moving animal are plotted as a function of time. (C) Quantitative analysis of mean fluorescent ratio changes of RME during forward (gray) and backward (red) locomotion. The mean value of R during forward locomotion was normalized as 100%. Figure S3. tbh-1 mutants exhibit normal calcium responses in RME during backward locomotion. A representative calcium trace of RME in tbh-1(ok1193) mutants. Figure S4. tph-1 and cat-2 mutants exhibit normal calcium responses in RME during backward locomotion. (A) Biosynthetic pathways of serotonin and dopamine. Genes encoding synthetic enzymes are shown under the arrows. (B, C) Calcium dynamics of RME in tph-1 (mg280) (B) and cat-2 (jq6) (C) mutants during spontaneous locomotion. Table S1. C. elegans strains used in this study. Table S2. Transgenic lines generated in this study. Table S3. Mutations and primers for genotyping. Table S4. Primers for molecular biology. (PDF 359 kb)

Published
2018
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22. Role of somite patterning in the formation of Weberian apparatus and pleural rib in zebrafish.

Author

Akama, Kagari, Ebata, Kanami, Maeno, Akiteru, Taminato, Tomohito, Otosaka, Shiori, Gengyo‐Ando, Keiko, Nakai, Junichi, Yamasu, Kyo, and Kawamura, Akinori

Subjects
OSSIFICATION, COMPUTED tomography, VERTEBRAE, BRACHYDANIO, SOMITE
Abstract

In the vertebrate body, a metameric structure is present along the anterior–posterior axis. Zebrafish tbx6−/− larvae, in which somite boundaries do not form during embryogenesis, were shown to exhibit abnormal skeletal morphology such as rib, neural arch and hemal arch. In this study, we investigated the role of somite patterning in the formation of anterior vertebrae and ribs in more detail. Using three‐dimensional computed tomography scans, we found that anterior vertebrae including the Weberian apparatus were severely affected in tbx6−/− larvae. In addition, pleural ribs of tbx6 mutants exhibited severe defects in the initial ossification, extension of ossification, and formation of parapophyses. Two‐colour staining revealed that bifurcation of ribs was caused by fusion or branching of ribs in tbx6−/−. The parapophyses in tbx6−/− juvenile fish showed irregular positioning to centra and abnormal attachment to ribs. Furthermore, we found that the ossification of the distal portion of ribs proceeded along myotome boundaries even in irregularly positioned myotome boundaries. These results provide evidence of the contribution of somite patterning to the formation of the Weberian apparatus and rib in zebrafish. [ABSTRACT FROM AUTHOR]

Published
2020
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23. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase is indispensable for oogenesis, oocyte-to-embryo transition, and larval development of the nematode Caenorhabditis elegans

Author

Kanaki, Nanako, primary, Matsuda, Ayako, additional, Dejima, Katsufumi, additional, Murata, Daisuke, additional, Nomura, Kazuko H, additional, Ohkura, Takashi, additional, Gengyo-Ando, Keiko, additional, Yoshina, Sawako, additional, Mitani, Shohei, additional, and Nomura, Kazuya, additional

Published
2018
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25. Additional file 1: Figure S1. of Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells

Author

Tanji, Takahiro, Nishikori, Kenji, Syoko Haga, Kanno, Yuki, Kobayashi, Yusuke, Takaya, Mai, Gengyo-Ando, Keiko, Mitani, Shohei, Shiraishi, Hirohisa, and Ohashi-Kobayashi, Ayako

Subjects
mental disorders, fungi
Abstract

Staining of Is[haf-4::GFP] (a) and Is[haf-9::GFP] (b) with the lysochrome dye Nile Red after fixation. Figure S2. DHS-3-positive intestinal granules are not autofluorescent granules. Figure S3. lmp-1 is not required for the biogenesis of DHS-3::GFP-positive lipid droplets. Figure S4. VIT-2::GFP did not localize to either the autofluorescent granules or the lipid droplets. Figure S5. LMP-1::mRFP did not localize to VIT-2::GFP-positive granules. Figure S6. GLO-1::GFP-positive granules are autofluorescent but not Nile Red-positive. Figure S7. LMP-1::mRFP did not localize to GLO-1::GFP-positive granules. Figure S8. glo-1 is not required for the biogenesis of DHS-3::GFP-positive lipid droplets. Figure S9. HAF-4 and HAF-9 did not localize to either the peroxisomes or the mitochondria. Figure S10. GFP::RAB-7 did not localize to either the autofluorescent granules or the Nile Red-positive lipid droplets. Figure S11. rab-7 is required for the biogenesis of LMP-1::mCherry-positive granules. Figure S12. rab-7 is not required for the biogenesis of autofluorescent granules and DHS-3::GFP-positive lipid droplets. Figure S13. Other representative images. (PDF 5985 kb)

Published
2016
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26. Control of sex-specific apoptosis in C. elegans by the BarH homeodomain protein CEH-30 and the transcriptional repressor UNC-37/Groucho

Author

Peden, Erin, Kimberly, Elizabeth, Gengyo-Ando, Keiko, Mitani, Shohei, and Ding Xue

Subjects
Apoptosis -- Research, Caenorhabditis elegans -- Genetic aspects, Caenorhabditis elegans -- Physiological aspects, Dimorphism (Biology) -- Research, Homeostasis -- Research, Biological sciences
Abstract

The various effects of the BarH homeodomain protein CEH-30 and the transcriptional repressor UNC-37/Groucho on the sex-specific apoptosis in Caenorhabditis elegans are discussed.

Published
2007

29. Calcium dynamics regulating the timing of decision-making in C. elegans

Author

Tanimoto, Yuki, primary, Yamazoe-Umemoto, Akiko, additional, Fujita, Kosuke, additional, Kawazoe, Yuya, additional, Miyanishi, Yosuke, additional, Yamazaki, Shuhei J, additional, Fei, Xianfeng, additional, Busch, Karl Emanuel, additional, Gengyo-Ando, Keiko, additional, Nakai, Junichi, additional, Iino, Yuichi, additional, Iwasaki, Yuishi, additional, Hashimoto, Koichi, additional, and Kimura, Koutarou D, additional

Published
2017
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30. Author response: Calcium dynamics regulating the timing of decision-making in C. elegans

Author

Tanimoto, Yuki, primary, Yamazoe-Umemoto, Akiko, additional, Fujita, Kosuke, additional, Kawazoe, Yuya, additional, Miyanishi, Yosuke, additional, Yamazaki, Shuhei J, additional, Fei, Xianfeng, additional, Busch, Karl Emanuel, additional, Gengyo-Ando, Keiko, additional, Nakai, Junichi, additional, Iino, Yuichi, additional, Iwasaki, Yuishi, additional, Hashimoto, Koichi, additional, and Kimura, Koutarou D, additional

Published
2017
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34. Erratum: Rational design of a high-affinity, fast, red calcium indicator R-CaMP2

Author

Inoue, Masatoshi, primary, Takeuchi, Atsuya, additional, Horigane, Shin-ichiro, additional, Ohkura, Masamichi, additional, Gengyo-Ando, Keiko, additional, Fujii, Hajime, additional, Kamijo, Satoshi, additional, Takemoto-Kimura, Sayaka, additional, Kano, Masanobu, additional, Nakai, Junichi, additional, Kitamura, Kazuo, additional, and Bito, Haruhiko, additional

Published
2015
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36. Rational design of a high-affinity, fast, red calcium indicator R-CaMP2

Author

Inoue, Masatoshi, primary, Takeuchi, Atsuya, additional, Horigane, Shin-ichiro, additional, Ohkura, Masamichi, additional, Gengyo-Ando, Keiko, additional, Fujii, Hajime, additional, Kamijo, Satoshi, additional, Takemoto-Kimura, Sayaka, additional, Kano, Masanobu, additional, Nakai, Junichi, additional, Kitamura, Kazuo, additional, and Bito, Haruhiko, additional

Published
2014
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37. Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling

Author

Nakamura, Fumio, primary, Kumeta, Kosuke, additional, Hida, Tomonobu, additional, Isono, Toshinari, additional, Nakayama, Yuichi, additional, Kuramata-Matsuoka, Emiko, additional, Yamashita, Naoya, additional, Uchida, Yutaka, additional, Ogura, Ken-ichi, additional, Gengyo-Ando, Keiko, additional, Mitani, Shohei, additional, Ogino, Toshio, additional, and Goshima, Yoshio, additional

Published
2014
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39. Distinct roles of the two VPS33 proteins in the endolysosomal system in Caenorhabditis elegans.

Author

Gengyo ‐ Ando, Keiko, Kage ‐ Nakadai, Eriko, Yoshina, Sawako, Otori, Muneyoshi, Kagawa ‐ Nagamura, Yuko, Nakai, Junichi, and Mitani, Shohei

Subjects
*CAENORHABDITIS elegans, *ENDOSOMES, *LYSOSOMES, *EUKARYOTIC cells, *MICROBIAL proteins, *PHENOTYPES, *ORIGIN of life
Abstract

Sec1/Munc-18 ( SM) family proteins are essential regulators in intracellular transport in eukaryotic cells. The SM protein Vps33 functions as a core subunit of two tethering complexes, class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) in the endocytic pathway in yeast. Metazoan cells possess two Vps33 proteins, VPS33A and VPS33B, but their precise roles remain unknown. Here, we present a comparative analysis of Caenorhabditis elegans null mutants for these proteins. We found that the vps-33.1 ( VPS33A) mutants exhibited severe defects in both endocytic function and endolysosomal biogenesis in scavenger cells. Furthermore, vps-33.1 mutations caused endocytosis defects in other tissues, and the loss of maternal and zygotic VPS-33.1 resulted in embryonic lethality. By contrast, vps-33.2 mutants were viable but sterile, with terminally arrested spermatocytes. The spermatogenesis phenotype suggests that VPS33.2 is involved in the formation of a sperm-specific organelle. The endocytosis defect in the vps-33.1 mutant was not restored by the expression of VPS-33.2, which indicates that these proteins have nonredundant functions. Together, our data suggest that VPS-33.1 shares most of the general functions of yeast Vps33 in terms of tethering complexes in the endolysosomal system, whereas VPS-33.2 has tissue/organelle specific functions in C. elegans. [ABSTRACT FROM AUTHOR]

Published
2016
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49. Two Golgi-resident 3′-Phosphoadenosine 5′-Phosphosulfate Transporters Play Distinct Roles in Heparan Sulfate Modifications and Embryonic and Larval Development in Caenorhabditis elegans

Author

Dejima, Katsufumi, primary, Murata, Daisuke, additional, Mizuguchi, Souhei, additional, Nomura, Kazuko H., additional, Izumikawa, Tomomi, additional, Kitagawa, Hiroshi, additional, Gengyo-Ando, Keiko, additional, Yoshina, Sawako, additional, Ichimiya, Tomomi, additional, Nishihara, Shoko, additional, Mitani, Shohei, additional, and Nomura, Kazuya, additional

Published
2010
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