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37 results on '"Gegechkori, Vladimir"'

11. An Overview of Adalimumab Therapy for Ankylosing Spondylitis

Author

Sukhanova, Anna M., primary, Gilavian, Mariam A., additional, Melnik, Elizaveta V., additional, Shikh, Evgenia V., additional, Petukhov, Alexey E., additional, Gegechkori, Vladimir I., additional, Dementev, Sergey P., additional, Vlasov, Alexander M., additional, and Ramenskaya, Galina V., additional

Published
2024
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14. Swelling, Rupture and Endosomal Escape of Biological Nanoparticles Per Se and Those Fused with Liposomes in Acidic Environment.

Author

Ponomareva, Natalia, Brezgin, Sergey, Karandashov, Ivan, Kostyusheva, Anastasiya, Demina, Polina, Slatinskaya, Olga, Bayurova, Ekaterina, Silachev, Denis, Pokrovsky, Vadim S., Gegechkori, Vladimir, Khaydukov, Evgeny, Maksimov, Georgy, Frolova, Anastasia, Gordeychuk, Ilya, Zamyatnin Jr., Andrey A., Chulanov, Vladimir, Parodi, Alessandro, and Kostyushev, Dmitry

Subjects
LYSOSOMES, LIPOSOMES, NANOPARTICLES, NUCLEIC acids, EXTRACELLULAR vesicles, SERS spectroscopy
Abstract

Biological nanoparticles (NPs), such as extracellular vesicles (EVs), exosome-mimetic nanovesicles (EMNVs) and nanoghosts (NGs), are perspective non-viral delivery vehicles for all types of therapeutic cargo. Biological NPs are renowned for their exceptional biocompatibility and safety, alongside their ease of functionalization, but a significant challenge arises when attempting to load therapeutic payloads, such as nucleic acids (NAs). One effective strategy involves fusing biological NPs with liposomes loaded with NAs, resulting in hybrid carriers that offer the benefits of both biological NPs and the capacity for high cargo loads. Despite their unique parameters, one of the major issues of virtually any nanoformulation is the ability to escape degradation in the compartment of endosomes and lysosomes which determines the overall efficiency of nanotherapeutics. In this study, we fabricated all major types of biological and hybrid NPs and studied their response to the acidic environment observed in the endolysosomal compartment. In this study, we show that EMNVs display increased protonation and swelling relative to EVs and NGs in an acidic environment. Furthermore, the hybrid NPs exhibit an even greater response compared to EMNVs. Short-term incubation of EMNVs in acidic pH corresponding to late endosomes and lysosomes again induces protonation and swelling, whereas hybrid NPs are ruptured, resulting in the decline in their quantities. Our findings demonstrate that in an acidic environment, there is enhanced rupture and release of vesicular cargo observed in hybrid EMNVs that are fused with liposomes compared to EMNVs alone. This was confirmed through PAGE electrophoresis analysis of mCherry protein loaded into nanoparticles. In vitro analysis of NPs colocalization with lysosomes in HepG2 cells demonstrated that EMNVs mostly avoid the endolysosomal compartment, whereas hybrid NPs escape it over time. To conclude, (1) hybrid biological NPs fused with liposomes appear more efficient in the endolysosomal escape via the mechanism of proton sponge-associated scavenging of protons by NPs, influx of counterions and water, and rupture of endo/lysosomes, but (2) EMNVs are much more efficient than hybrid NPs in actually avoiding the endolysosomal compartment in human cells. These results reveal biochemical differences across four major types of biological and hybrid NPs and indicate that EMNVs are more efficient in escaping or avoiding the endolysosomal compartment. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Study of the silver birchpollen allergoid

Author

Kulaga, Olga S., primary, Avoyan, Gayane E., additional, Esaulova, Daria R., additional, Andreev, Igor V., additional, Nechay, Ksenia O., additional, Andreev, Alexandr I., additional, Kicheeva, Karina B., additional, Baklakova, Olga S., additional, Mislavsky, Oleg V., additional, Gegechkori, Vladimir I., additional, Cherchenko, Nikolay G., additional, Sankov, Mikhail N., additional, Toptygin, Аndrey Yu., additional, Shvets, Svetlana M., additional, Romanova, Tatiana S., additional, Latysheva, Elena A., additional, Martynov, Аlexander I., additional, and Khaitov, Musa R., additional

Published
2022
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28. Essential Oil Quality and Purity Evaluation via FT-IR Spectroscopy and Pattern Recognition Techniques

Author

Agatonovic-Kustrin, Snezana, Ristivojević, Petar, Gegechkori, Vladimir, Litvinova, Tatiana M., W. Morton, David, Agatonovic-Kustrin, Snezana, Ristivojević, Petar, Gegechkori, Vladimir, Litvinova, Tatiana M., and W. Morton, David

Abstract

Essential oils are highly volatile, aromatic concentrated extracts from plants with wide applications. In this study, fast, easy-to-use attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was combined with chemometric techniques to verify essential oils’ taxonomy and purity. Principal component analysis (PCA) clustered 30 essential oil samples into three different groups based on plant botanical family and concentration. The first group contained highly concentrated oils from the Asteraceae family, the second group contained highly concentrated oils from the Lamiaceae family, while the last group contained three highly concentrated essential oils from different botanical families and commercial-grade essential oils. Thus, commercial-grade oil samples did not cluster with the corresponding concentrated oil samples despite their similar spectral patterns or botanical family. A loading plot identified infrared (IR) bands that correspond to carbonyl, vinyl, methyl and methylene group vibrations as the most important spectral bands that can be used as marker bands for discrimination between different botanical plant family groups. Hierarchical cluster analysis (HCA) confirmed the results obtained by PCA. ATR-FTIR spectroscopy combined with chemometric algorithms provides a direct and non-destructive method for chemotaxonomic classification of medicinal and aromatic essential oils and an assessment of their purity.

Published
2020

29. COLCHICINE AS A NATURAL TOXICANT AND METHODS OF ITS ANALYSIS

Author

Belova Maria Vladimirovna, Gegechkori Vladimir Iraklievich

Abstract

Colchicine is an indispensable medical alkaloid of the Colchicaceae family. It is still used in the treatment of gout, recurrent illness, Behcet's disease and many other diseases. Despite the centuries-old experience of using this alkaloid by humans, fatal cases of poisoning are still encountered. Materials and methods. The authors analyzed specialized literature in databases and search engines Google Scholar, PubMed, Web of Science, Scopus, eLibrary on the toxicological, pharmacological, technological and clinical significance of colchicine. Results. Pharmacodynamics, clinical signs and pathological changes in case of colchicine poisoning are described based on the data of contemporary literature. In addition, the basic principles of the qualitative and quantitative determination of substances from biological material are illustrated. Keywords: colchicine; Colchicum autumnale; toxicology; poisoning; extraction.

Published
2019
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37. Production of biomedical cell products: requirements for the quality of donor material and excipients of animal origin (review)

Author

Vodiakova, Marina A., Sayfutdinova, Aliya R., Melnikova, Ekaterina V., Goryaev, Artem A., Sadchikova, Natalia P., Gegechkori, Vladimir I., and Merkulov, Vadim A.

Abstract

Donor biological materials and excipients of animal origin are important components in the production of biomedical cell products (BMCPs). Their quality ensures the stability, safety, effectiveness and purity of the final product. This review discusses quality requirements for biological excipients intended for the production of biomedical cell products, in terms of the necessary information that should be included in the BMCP registration dossier during state registration and is subject to expert assessment during quality control. Considering that there is currently no production of biomedical cell products in the Russian Federation, the authors considered international approaches to ensuring the safety of donor material and excipients for the manufacturing of human cell- and tissue-based products (BMCP analogues).

Published
2020
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