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291 results on '"Geczy, Carolyn L."'

1. Colchicine promotes atherosclerotic plaque stability independently of inflammation

Author

Li, Weizhen, primary, Lin, Alexander, additional, Hutton, Michael, additional, Dhaliwal, Harkirat, additional, Nadel, James, additional, Rodor, Julie, additional, Tumanov, Sergey, additional, Örd, Tiit, additional, Hadden, Matthew, additional, Mokry, Michal, additional, Mol, Barend M, additional, Pasterkamp, Gerard, additional, Padula, Matthew P, additional, Geczy, Carolyn L, additional, Ramaswamy, Yogambha, additional, Sluimer, Judith C, additional, Kaikkonen, Minna U, additional, Stocker, Roland, additional, Baker, Andrew H, additional, Fisher, Edward A, additional, Patel, Sanjay, additional, and Misra, Ashish, additional

Published
2023
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3. Colchicine promotes atherosclerotic plaque stability independently of inflammation

Author

CDL Onderzoek Pasterkamp, Experimentele Afd. Cardiologie 1, Child Health, Circulatory Health, Zorgeenheid Vaatchirurgie Medisch, Centraal Diagnostisch Laboratorium, Li, Weizhen, Lin, Alexander, Hutton, Michael, Dhaliwal, Harkirat, Nadel, James, Rodor, Julie, Tumanov, Sergey, Örd, Tiit, Hadden, Matthew, Mokry, Michal, Mol, Barend M, Pasterkamp, Gerard, Padula, Matthew P, Geczy, Carolyn L, Ramaswamy, Yogambha, Sluimer, Judith C, Kaikkonen, Minna U, Stocker, Roland, Baker, Andrew H, Fisher, Edward A, Patel, Sanjay, Misra, Ashish, CDL Onderzoek Pasterkamp, Experimentele Afd. Cardiologie 1, Child Health, Circulatory Health, Zorgeenheid Vaatchirurgie Medisch, Centraal Diagnostisch Laboratorium, Li, Weizhen, Lin, Alexander, Hutton, Michael, Dhaliwal, Harkirat, Nadel, James, Rodor, Julie, Tumanov, Sergey, Örd, Tiit, Hadden, Matthew, Mokry, Michal, Mol, Barend M, Pasterkamp, Gerard, Padula, Matthew P, Geczy, Carolyn L, Ramaswamy, Yogambha, Sluimer, Judith C, Kaikkonen, Minna U, Stocker, Roland, Baker, Andrew H, Fisher, Edward A, Patel, Sanjay, and Misra, Ashish

Published
2023

4. S100 Proteins

Author

Donato, Rosario, Geczy, Carolyn L., Weber, David J., Kretsinger, Robert H., editor, Uversky, Vladimir N., editor, and Permyakov, Eugene A., editor

Published
2013
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6. Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment

Author

Wong, Sze Wing, McCarroll, Joshua, Hsu, Kenneth, Geczy, Carolyn L., and Tedla, Nicodemus

Subjects
Mice, Lung Neoplasms, Immunology, Tumor Microenvironment, Immunology and Allergy, Animals, Proteins, Calgranulin A, Thorax, Lung
Abstract

Lung cancer is the leading cause of cancer-related death worldwide. Increasing evidence indicates a critical role for chronic inflammation in lung carcinogenesis. S100A8 is a protein with reported pro- and anti-inflammatory functions. It is highly expressed in myeloid-derived suppressor cells (MDSC) that accumulate in the tumor microenvironment and abrogate effective anti-cancer immune responses. Mechanisms of MDSC-mediated immunosuppression include production of reactive oxygen species and nitric oxide, and depletion of L-arginine required for T cell function. Although S100A8 is expressed in MDSC, its role in the lung tumor microenvironment is largely unknown. To address this, mouse recombinant S100A8 was repeatedly administered intranasally to mice bearing orthotopic lung cancers. S100A8 treatment prolonged survival from 19 days to 28 days (p < 0.001). At midpoint of survival, whole lungs and bronchoalveolar lavage fluid (BALF) were collected and relevant genes/proteins measured. We found that S100A8 significantly lowered expression of cytokine genes and proteins that promote expansion and activation of MDSC in lungs and BALF from cancer-bearing mice. Moreover, S100A8 enhanced activities of antioxidant enzymes and suppressed production of nitrite to create a lung microenvironment conducive to cytotoxic lymphocyte expansion and function. In support of this, we found decreased MDSC numbers, and increased numbers of CD4+T cells and natural killer T (NK-T) cells in lungs from cancer-bearing mice treated with S100A8.Ex-vivotreatment of splenocytes with S100A8 protein activated NK cells. Our results indicate that treatment with S100A8 may favourably modify the lung microenvironment to promote an effective immune response in lungs, thereby representing a new strategy that could complement current immunotherapies in lung cancer.

Published
2021

24. Serum Amyloid A Stimulates Vascular and Renal Dysfunction in Apolipoprotein E-Deficient Mice Fed a Normal Chow Diet

Author

Chami, Belal, primary, Hossain, Farjaneh, additional, Hambly, Thomas W., additional, Cai, Xiaoping, additional, Aran, Roshanak, additional, Fong, Genevieve, additional, Vellajo, Abigail, additional, Martin, Nathan J.J, additional, Wang, XiaoSuo, additional, Dennis, Joanne M., additional, Sharma, Arpeeta, additional, Shihata, Waled A., additional, Chin-Dusting, Jaye P. F., additional, de Haan, Judy B., additional, Sharland, Alexandra, additional, Geczy, Carolyn L., additional, Freedman, Ben, additional, and Witting, Paul K., additional

Published
2019
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27. Mass spectrometric analysis of electrophoretically separated allergens and proteases in grass pollen diffusates

Author

Geczy Carolyn L, Saldanha Rohit G, Raftery Mark J, and Kumar Rakesh K

Subjects
Diseases of the respiratory system, RC705-779
Abstract

Abstract Background Pollens are important triggers for allergic asthma and seasonal rhinitis, and proteases released by major allergenic pollens can injure airway epithelial cells in vitro. Disruption of mucosal epithelial integrity by proteases released by inhaled pollens could promote allergic sensitisation. Methods Pollen diffusates from Kentucky blue grass (Poa pratensis), rye grass (Lolium perenne) and Bermuda grass (Cynodon dactylon) were assessed for peptidase activity using a fluorogenic substrate, as well as by gelatin zymography. Following one- or two-dimensional gel electrophoresis, Coomassie-stained individual bands/spots were excised, subjected to tryptic digestion and analysed by mass spectrometry, either MALDI reflectron TOF or microcapillary liquid chromatography MS-MS. Database searches were used to identify allergens and other plant proteins in pollen diffusates. Results All pollen diffusates tested exhibited peptidase activity. Gelatin zymography revealed high Mr proteolytic activity at ~ 95,000 in all diffusates and additional proteolytic bands in rye and Bermuda grass diffusates, which appeared to be serine proteases on the basis of inhibition studies. A proteolytic band at Mr ~ 35,000 in Bermuda grass diffusate, which corresponded to an intense band detected by Western blotting using a monoclonal antibody to the timothy grass (Phleum pratense) group 1 allergen Phl p 1, was identified by mass spectrometric analysis as the group 1 allergen Cyn d 1. Two-dimensional analysis similarly demonstrated proteolytic activity corresponding to protein spots identified as Cyn d 1. Conclusion One- and two-dimensional electrophoretic separation, combined with analysis by mass spectrometry, is useful for rapid determination of the identities of pollen proteins. A component of the proteolytic activity in Bermuda grass diffusate is likely to be related to the allergen Cyn d 1.

Published
2003
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32. Soluble LILRA3 promotes neurite outgrowth and synapses formation through high affinity interaction with Nogo 66

Author

An, Hongyan, primary, Brettle, Merryn, additional, Lee, Terry, additional, Heng, Benjamin, additional, Lim, Chai K., additional, Guillemin, Gilles J., additional, Lord, Megan S., additional, Klotzsch, Enrico, additional, Geczy, Carolyn L., additional, Bryant, Katherine, additional, Fath, Thomas, additional, and Tedla, Nicodemus, additional

Published
2016
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38. Importance of C-reactive protein in regulating monocyte tissue factor expression in patients with inflammatory rheumatic diseases

Author

Hong Cai, Changjie Song, Irwin Geok San Lim, Krilis, Steven A., Geczy, Carolyn L., and Hugh Patrick McNeil

Subjects
Adult, Lipopolysaccharides, Male, Arteriosclerosis, Middle Aged, Monocytes, Thromboplastin, Up-Regulation, Arthritis, Rheumatoid, C-Reactive Protein, beta 2-Glycoprotein I, Humans, Female, Cells, Cultured, Glycoproteins
Abstract

To determine the relationship between plasma C-reactive protein (CRP) concentrations and monocyte tissue factor (TF) expression induced in vitro by combinations of CRP, ss2-glycoprotein I (ss2-GPI), and lipopolysaccharide (LPS).Peripheral blood mononuclear cells (PBMC) from 26 healthy individuals and 31 patients with inflammatory rheumatic diseases (IRD) were cultured with combinations of CRP, purified or recombinant ss2-GPI, and LPS and monocyte TF procoagulant activity, TF antigen, and TF mRNA were measured. Results were examined against plasma CRP levels.Monocytes from patients with IRD expressed significantly more TF when stimulated with CRP compared to normal monocytes (p = 0.002). An incremental positive correlation was observed between plasma CRP levels and TF induced by CRP or ss2-GPI. Significantly more TF was induced with CRP combined with ss2-GPI, compared to ss2-GPI alone, either with costimulation or CRP priming. Conversely, when combined with LPS, ss2-GPI suppressed TF induction in a dose-dependent manner on normal PBMC but not on PBMC from patients with IRD. The loss of suppression correlated strongly with plasma CRP levels.This study shows a remarkably consistent effect of CRP on monocyte TF expression. Systemic inflammation associated with elevated plasma CRP conferred a phenotype on PBMC, whereby incremental priming with respect to TF expression (induced by CRP itself or ss2-GPI) was apparent, and ss2-GPI-mediated inhibition of TF expression induced by LPS was incrementally lost. CRP regulation of monocyte TF could contribute to the higher than expected atherosclerotic vascular disease seen in patients with IRD.

Published
2005

39. 79

Author

Hiroshima, Yuka, primary, Hsu, Kenneth, additional, Tedla, Nicodemus, additional, Chow, Sharron, additional, Kawaguchi, Naomi, additional, and Geczy, Carolyn L., additional

Published
2014
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40. 83

Author

Hsu, Kenneth, primary, Hiroshima, Yuka, additional, Endoh, Yasumi, additional, Thomas, Shane R., additional, and Geczy, Carolyn L., additional

Published
2014
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45. Soluble LILRA3 promotes neurite outgrowth and synapses formation through a high-affinity interaction with Nogo 66.

Author

Hongyan An, Brettle, Merryn, Lee, Terry, Heng, Benjamin, Lim, Chai K., Guillemin, Gilles J., Lord, Megan S., Klotzsch, Enrico, Geczy, Carolyn L., Bryant, Katherine, Fath, Thomas, and Tedla, Nicodemus

Subjects
NEURONS, NOGO protein, SYNAPSES, LEUCOCYTES, IMMUNOGLOBULINS, CELL receptors
Abstract

Inhibitory proteins, particularly Nogo 66, a highly conserved 66-amino-acid loop of Nogo A (an isoform of RTN4), play key roles in limiting the intrinsic capacity of the central nervous system (CNS) to regenerate after injury. Ligation of surface Nogo receptors (NgRs) and/or leukocyte immunoglobulin-like receptor B2 (LILRB2) and its mouse orthologue the paired immunoglobulin-like receptor B (PIRB) by Nogo 66 transduces inhibitory signals that potently inhibit neurite outgrowth. Here, we show that soluble leukocyte immunoglobulin-like receptor A3 (LILRA3) is a high-affinity receptor for Nogo 66, suggesting that LILRA3 might be a competitive antagonist to these cell surface inhibitory receptors. Consistent with this, LILRA3 significantly reversed Nogo-66-mediated inhibition of neurite outgrowth and promoted synapse formation in primary cortical neurons through regulation of the ERK/MEK pathway. LILRA3 represents a new antagonist to Nogo-66-mediated inhibition of neurite outgrowth in the CNS, a function distinct from its immune-regulatory role in leukocytes. This report is also the first to demonstrate that a member of LILR family normally not expressed in rodents exerts functions on mouse neurons through the highly homologous Nogo 66 ligand. [ABSTRACT FROM AUTHOR]

Published
2016
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