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3. Blue light exposure intensifies leaf red pigmentation and enhances oxidative stress tolerance in the ornamental bromeliad Neoregelia ‘Fireball’

Author

Shi, Jiewei, primary, Zhan, Shuxia, additional, Jin, Liang, additional, Zhou, Qin, additional, Shen, Yuxiao, additional, Wan, Xiao, additional, Zou, Longhai, additional, Dong, Qing, additional, Bao, Manzhu, additional, Tian, Danqing, additional, Ning, Guogui, additional, and Ge, Yaying, additional

Published
2023
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5. Cloning and Sequence Analysis of Actin Gene from Guzmania

Author

Wang Weiyong, Liu Jianxin, Tian Danqing, Ding Huaqiao, Ge Yaying, Zhang Zhi, and Shen Fuquan

Subjects
Genetics, Open reading frame, Sequence analysis, GenBank, Complementary DNA, Primer walking, food and beverages, Biology, Molecular biology, Protein secondary structure, Gene, Protein tertiary structure
Abstract

As a house keeping gene, Actin was often used as inner control in quantitative and semiquantitative PCR tests to determine relative expressive amount of target genes. Based on EST monoclones of Actin gene obtained from Guzmania full length cDNA library, its full length cDNA sequence was obtained by Primer Walking sequencing. The gene, named for Goactin1 (GenBank accession No. HQ184438), consisted of 1 625 bp cDNA sequence, 1 131 bp ORF (open reading frame) which encoded a protein with 377 amino acids residues, and a putative protein which was 41.7 kD at estimated molecular weight, 5.31 at isoelectric point and had a ‘actin superfamily’conservative domain. The secondary structure of the protein was composed of random coil, alpha helix, extend strand and beta turn by SPOMA analysis. Furthermore, its tertiary structure was also build based on A chain of 2BTF. Through phylogenetic tree analysis, Goactin1 was gather to a same group with Actin protein in Solanum tuberosum , Gossypium hirsutum , Nicotiana tabacum , Brachypodium sylvaticum , and Arabidopsis thaliana .

Published
2011

10. Genetic relatedness among Aechmea species and hybrids inferred from AFLP markers and pedigree data

Author

Zhang, Fei, Wang, Weiyong, Ge, Yaying, Shen, Xiaolan, Tian, Danqing, Liu, Jianxin, Liu, Xiaojing, Yu, Xinyin, and Zhang, Zhi

Subjects
*BROMELIACEAE, *PLANT genetics, *PLANT species, *BIOMARKERS, *AMPLIFIED fragment length polymorphism, *CULTIVARS
Abstract

Abstract: Bromeliads are among the most economically important potted ornamentals, however little is available about genetic variability among bromeliad cultivars or hybrids for breeding purposes. In this study, genetic relatedness among cultivars and selected hybrids of Aechmea bromeliad was investigated using amplified fragment length polymorphism (AFLP) markers and pedigree data. Eight AFLP primer combinations produced 1498 DNA fragments, of which 1465 (97.8%) were polymorphic. The AFLP-based genetic similarity ranged from 0.20 to 0.61 and averaged 0.33, while the corresponding pedigree-based coefficient of coancestry varied from 0 to 0.5, with an average of 0.07. This reveals large variability and genetic diversity among the investigated Aechmea bromeliads. The AFLP-based cluster analysis grouped the Aechmea bromeliads well according to their breeding years and ancestors, whereas pedigree-based cluster analysis divided the Aechmea bromeliads corresponding only to their ancestors. Additionally, the AFLP-based genetic similarity followed a normal distribution, while pedigree-based coefficient of coancestry displayed a skewed distribution. A significant though moderate correlation (r =0.35, P <0.001) and a significant linear relationship with low coefficient of determination (R 2 =0.12) were observed between the two measures. This indicates that AFLP is more effective in quantifying genetic relatedness among Aechmea bromeliads. The present study provides useful implications for the efficient conservation and utilization of bromeliad germplasm. [Copyright &y& Elsevier]

Published
2012
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