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Additional file 1. Table S1: Information of brain donors in this study. Fig. S1: The expression pattern of S6K1 in HEK293 cells and neurons. Fig. S2: The quantification of PSD95 and synaptophysin in fractions and the validation of p70S6K antibodies. Fig. S3: Knockdown of p85S6K efficiently reduces p85S6K in PSD-1 and does not affect the locomotor activity and anxiety-like behavior. Fig. S4: p85S6K cannot be immunoprecipitated with GluA1 with little expression of AKAP79 in HEK293 cells. Fig. S5: p85S6K does not interfere with the interaction between GluA1 and PKA. Fig. S6: The immunoblots of p85S6K/p70S6K and phosphorylated form in remaining human temporal cortex samples related to Fig. 5a. Fig. S7: p85S6K expression in P2 pellets of cortex of APP/PS1 mice. Fig. S8: p85S6K expression is decreased in PSD-1 of cortex of 5×FAD mice. Fig. S9: PSD95 expression is not altered in AD brains. Fig. S10: Overexpression of p85S6K does not affect the locomotor activity and anxiety-like behavior. Fig. S11: Upregulation of p85S6K does not enhance the spatial learning and spine density in S845A mice. Fig. S12: The NLS predicted in p85S6K by www.csbio.sjtu.edu.cn/bioinf/INSP/ . Fig. S13: The expression of GluA1 and its phosphorylation at Ser845 are reduced in P2 pellets from fractionation of cortex of 7-month old 5×FAD and 9-month old APP/PS1 mice.

Qiyue mountain tunnel lies in Hurongxi highway in Hubei Province, soluble rocks are karstified intensively. To predict and prevent water inrush into tunnel when it is constructed, risk measurement is necessary to guide comprehensive advance geology prediction and prevention measures. Fuzzy mathematics model is put forward to distinguish potentially dangerous parts along tunnel. 7 main controls of rock karstification are selected as risk rank indicators. Use the fuzzy mathematics model differentiated the tunnel into 4 ranks of dangerous parts according to risk index. It is applied in Qiyue mountain tunnel and practice approves that the measurement and measures are useful.