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Additional file 1: Table S1. Primary antibodies used in western blot experiment. Table S2. HR-ESI-MS data of compounds. Fig. S1. HPLC-DAD (A) and positive-mode (B) LCMS-IT-TOF chromatograms of RAF, and HPLC-DAD (C) and positive-mode (D) LCMS-IT-TOF chromatograms of PAF. Peaks 1: N-trans-p-coumaroy lnoradrenline, 2: N-trans-p-coumaroyloctopamine, 3: magnoflorine, 4: N-trans-feruloyloctopamine, 5: berberine, 6: dehydrocheilanthifo-line, 7: isomer-dehydrocheilanthifoline, 8: tetrahydropalmatine, 9: bicuculine, 10: 6,7-methylenedioxy-2-(6-acetyl-2,3-methylenedioxybenzyl)-1(2H)-isoquinolinone, 11: protopine, 12: allocryptopine, 13: hendersine B, 14: stylopine. Fig. S2. Chemical structures of 14 alkaloids in RAF of Corydalis hendersonii Hemsl. Fig. S3. (A) The cytotoxicity of RAF on H9c2 cells was detected by CCK-8 assay. Data are represented as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001, compared with the ctrl (control) group. (B) Detection of H9c2 cell viability after incubation in hypoxic serum-free medium for 8 h. Data are represented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the Model group.

Background: Diabetic mellitus erectile dysfunction (DMED) refers to erectile dysfunction (ED) secondary to diabetes. As people's lifestyle changes and the population ages, the incidence of DMED continues to increase. Many clinical trials have proven that Chinese medicine has a significant effect in the treatment of DMED. In this systematic review, we aim to evaluate the effectiveness and safety of traditional Chinese medicine (TCM) for DMED. Methods: We will search PubMed, Cochrane Library, AMED, EMbase, WorldSciNet; Nature, Science online and China Journal Full-text Database, China Biomedical Literature CD-ROM Database, and related randomized controlled trials included in the China Resources Database. The time is limited from the construction of the library to February, 2019.We will use the criteria provided by Cochrane 5.1.0 for quality assessment and risk assessment of the included studies, and use the Revman 5.3 and Stata13.0 software for meta-analysis of the effectiveness, recurrence rate, and symptom scores of DMED. Ethics and dissemination: This systematic review will evaluate the efficacy and safety of TCM for treating Diabetic mellitus erectile dysfunction. Because all of the data used in this systematic review and meta-analysis has been published, this review does not require ethical approval. Furthermore, all data will be analyzed anonymously during the review process trial.