1. [MAST2-like protein kinase from grape vine Vitis vinifera: cloning of catalytic domain cDNA].
- Author
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Briantseva SA, Gavriushina ES, Emets AI, Karpov PA, Blium IaB, Drygin IuF, and Nadezhdina ES
- Subjects
- Amino Acid Sequence, Catalytic Domain, Cloning, Molecular, Gene Library, Humans, Microtubules enzymology, Microtubules ultrastructure, Molecular Sequence Data, Plant Leaves enzymology, Plant Leaves ultrastructure, Polymerase Chain Reaction, Sequence Alignment, Vitis ultrastructure, DNA, Complementary genetics, Microtubule-Associated Proteins genetics, Protein Serine-Threonine Kinases genetics, Vitis enzymology
- Abstract
The aim of our work is the identification of protein kinases phosphorylating microtubule proteins in plant cells. Using bioinformatic approach, we found genes of putative homologues of microtubule-associated mammalian protein kinase MAST2 in higher plant genomes. The gene of closest MAST2 homologue, putative protein, named GMLK (Grape MAST2-Like Kinase, A7NTE9_VITVI), was found in grape Vitis vinifera. We report here the cloning of cDNA of GMLK (A7NTE9) from Pinot Noir grape vine leaves.
- Published
- 2010