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Background. While examining regional lymph nodes removed with the tumor, the pathomorphologist is often limited to the exclusion of metastases, omitting changes in lymphoid tissue. The aim of the work is to describe a rare variant of Castleman-like lymphadenopathy in the surgical material obtained during the removal of colon cancer with regional lymph nodes. Methods. Pathomorphological examination of removed lymph nodes using histological and immunohistochemical methods (CD23). Results. A 73-year-old patient underwent surgery for rectal cancer. Pathomorphological analysis diagnosed adenocarcinoma of high degree of differentiation (G1) and revealed Castleman-like changes in some of the enlarged regional mesenteric lymph nodes: signs of different stages of development of lymphoid follicles, including their hyperplasia, atresia and fragmentation with preservation of activated CD23+ follicular dendritic cells and reaction of microvasculature, including vascular proliferation in the interfollicular areas with uneven narrowing of the sinuses. The discussion analyzes the probable genesis of changes in lymphoid tissue, which may be a reflection of the early stage of Castleman's disease, complication of radiation therapy performed in the preoperative stage, or a consequence of impaired immune status due to tumor growth, in particular with antigenic overloading of draining lymphoid tissue, effects of cytokines produced by inflammatory infiltrate cells that accumulate directly near the tumor, and the formation of a state of genetic instability, which progresses under the influence of radiation. Conclusion. Despite the rarity of Castleman-like lymphadenopathy, in each case of examination of lymph nodes in the surgical material it is necessary to conduct a thorough analysis of lymphoid tissue, as this may be important both to assess the prognosis and to select adequate therapy.

Практика показує, що чистопородні бджолині родини в умовах товарних пасік часто не можуть реалізувати свій генетичний потенціал. А породи бджіл, які склалися в процесі еволюції пристосувалися до конкретних природно-кліматичних умов. Тільки правильне використання, з урахуванням біологічних і господарсько-корисних ознак бджіл, сприяє успішному розвитку бджільництва., Практика показывает, что чистопородные пчелиные семьи в условиях товарных пасек часто не могут реализовать свой генетический потенциал. А породы пчел, которые сложились в процессе эволюции приспособились к конкретным природно-климатическим условиям. Только правильное использование, с учетом биологических и хозяйственно-полезных признаков пчел, способствует успешному развитию пчеловодства., Practice shows that purebred bee colonies in conditions of commodity apiaries often can not realize their genetic potential. And the breeds of bees that have evolved during the evolution have adapted to the specific natural and climatic conditions. Only proper use, taking into account the biological and economically useful attributes of bees, contributes to the successful development of beekeeping.

Endometrial cancer risk prediction models including lifestyle, anthropometric and reproductive factors have limited discrimination. Adding biomarker data to these models may improve predictive capacity; to our knowledge, this has not been investigated for endometrial cancer. Using a nested case-control study within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, we investigated the improvement in discrimination gained by adding serum biomarker concentrations to risk estimates derived from an existing risk prediction model based on epidemiologic factors. Serum concentrations of sex steroid hormones, metabolic markers, growth factors, adipokines and cytokines were evaluated in a step-wise backward selection process; biomarkers were retained at p < 0.157 indicating improvement in the Akaike information criterion (AIC). Improvement in discrimination was assessed using the C-statistic for all biomarkers alone, and change in C-statistic from addition of biomarkers to preexisting absolute risk estimates. We used internal validation with bootstrapping (1000-fold) to adjust for over-fitting. Adiponectin, estrone, interleukin-1 receptor antagonist, tumor necrosis factor-alpha and triglycerides were selected into the model. After accounting for over-fitting, discrimination was improved by 2.0 percentage points when all evaluated biomarkers were included and 1.7 percentage points in the model including the selected biomarkers. Models including etiologic markers on independent pathways and genetic markers may further improve discrimination.

Background and aim. Alcoholic steatohepatitis (ASH), nonalcoholic steatohepatitis (NASH) and hepatitis C virus infection (HCV) are the most frequent causes of liver cirrhosis in the Western world. The course of the disease depends on the balance in fibrogenesis, angiogenesis and regeneration. But if fibrosis was studied intensively during the last decades, mechanisms and signs of reparative regeneration in these diseases remain unknown. Ductular reaction is one of the morphologic signs of regeneration, but it was examined mostly in diseases associated with cholestasis. The aim of our work is to study ductular reaction associated with expression of immunohistochemical marker CK19 in alcoholic steatohepatitis, nonalcoholic steatohepatitis and hepatitis C virus infection.Material and methods. 45 autopsies with diagnosed alcoholic steatohepatitis, nonalcoholic steatohepatitis and viral hepatitis C were enrolled in this study. Diagnosis of ASH was based on the data of alcohol abuse and morphologic signs of alcoholic disease – cardiomyopathy, chronic pancreatitis, alcoholic encephalopathy and typical liver changes. Viral genesis was proved by serological study (RNA HCV) and morphologic signs of HCV (METAVIR criteria). Diagnosis of NASH was verified by the features of metabolic syndrome and hepatic changes (Brunt criteria). The employed measures of reparative regeneration were CK19-positive cells with “biliary phenotype”, evaluated in the liver tissue (area of ductular reaction). Marker quantification was performed by assessing the ratio of stained tissue to the total area of the liver section using image analysis. Histotopographic features of ductular reaction and cellular phenotype were also studied. “STATISTICA FOR WINDOWS6.0” was used to analyze the data. The data were expressed as mean value ± standard deviation (M±s). Differences between groups were analyzed using ANOVA analysis (LSD test). P < 0,05 was considered significant.Results. Evaluation of CK19-positive c

Background. Angiogenesis together with fibrogenesis and regeneration is an important mechanism of tissue reorganization in chronic liver diseases. Objective of the study is to examine morphologic signs of angiogenesis at the stage of cirrhotic transformation in alcoholic steatohepatitis, nonalcoholic steatohepatitis and hepatitis C virus infection. Methods. 45 autopsies with diagnosed alcoholic steatohepatitis, nonalcoholic steatohepatitis and viral hepatitis C were enrolled in this study. Diagnosis of alcoholic steatohepatitis was based on the data of alcohol abuse and morphologic signs of alcoholic disease – cardiomyopathy, chronic pancreatitis, alcoholic encephalopathy and typical liver changes. Viral genesis was proved by serological study (RNA HCV) and morphologic signs of HCV (METAVIR criteria). Diagnosis of nonalcoholic steatohepatitis was verified by the features of metabolic syndrome and hepatic changes (Brunt critera). The measures of angiogenesis employed were CD34-positive cells, evaluated in the liver tissue. Marker quantification was performed by assessing the ratio of stained tissue to the total area of the liver section using image analysis. “STATISTICA FOR WINDOWS 6.0” was used to analyze the data. Differences between groups were analyzed using ANOVA analysis (LSD test). P < 0,05 was considered significant. Results. General angiogenesis index was the highest in viral hepatitis and the lowest in nonalcoholic steatohepatitis (p<0.05). The same tendency was revealed for septal indexes: in viral hepatitis the meaning was three times higher than in steatohepatitis (difference between alcoholic and nonalcoholic steatohepatitis was not statistically significant). The highest lobular angiogenesis index was revealed in nonalcoholic steatohepatitis and the lowest in viral hepatitis (p<0.05). Conclusion. In hepatitis C virus infection CD34-positive cells are seen mostly in the septal compartment and in chronic steatohepatitis (alcoholic and nonalcohol