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Additional file 10: FigureS10. GWAS Manhattan plots for (a) Stearic acid; (b) Nonadecanoic acid; (c) Eicosenoic acid; (d) Docosanoic acid; (e) Tetracosanoic acid; (f) Nervonic acid; (g) Oleic acid; (h) Linoleic acid; (i) Ratio between oleic and linoleic acids. FDR and Bonferroni thresholds are shown by blue and red, respectively.

Additional file 5: FigureS5. (A) Multidimensional scaling plot (two dimensions, 1 - Spearman correlation coefficient between TAGs abundances measured using LC-MS with dilution 1:25 was used as distance). One sample is shown by one point; accessions are shown by different colors; different years are shown by points of different shapes. (B) Minus log10 p-values for the differences between lines (ANOVA) are shown, TAGs are ordered by p-value increase from top left to bottom right. Bonferroni adjusted 0.05 significance level is shown by red line; (C-G) abundances of five selected TAGs are shown across lines and years. Each point represents 1 sample, point shapes, and colors as in (A), lines show per-year averages. This figure complements main Fig. 3.

Additional file 9: FigureS9. Replication experiment on 6 accessions. (Left) Distributions of FAs (top) or TAGs (middle and bottom) by percentages of variance explained by genotype (G), environment (year, E), interaction between genotype and environment (G:E) or percentage of residual variance. Distribution of the same percentages divided by number of degrees of freedom of corresponding factor are shown on the right.

Additional file 6: FigureS6. Replication experiment on 6 accessions: relative abundances of all the fatty acids with significant (FDR

Additional file 3: FigureS3. Joint principal component analysis of sunflower accessions genotyped in this study and in Hübner (2019) based on 2345 shared SNPs. The first and the second (A) or the first and the third (B) PCs are shown. Each dot corresponds to a plant accession. Colors indicate the origin (wild/line/landrace). Shapes indicate species.

Additional file 8: FigureS8. Replication experiment on 6 genotypes: relative abundances of all TAGs detected by LC-MS with dilution 1:25 with significant (FDR

Additional file 7: FigureS7. Replication experiment on 6 genotypes: relative abundances of all TAGs detected by LC-MS with dilution 1:3 with significant (FDR

Additional file 2: FigureS2. Linkage disequilibrium (LD) decay plot. (A) Genome-wide LD. Gray dots correspond to a SNP pair, y-axis show r2 between two SNPs calculated using whole dataset. SNP pairs with distance less than 5 Mb are shown. (B) LD per each chromosome. Lines correspond to loess curves; 95% confidence intervals are shown by shades.

Additional file 4 (A) Multidimensional scaling plot (two dimensions, 1 - Spearman correlation coefficient between TAGs abundances measured using LC-MS with dilution 1:3 was used as distance). One sample is shown by one point; accessions are shown by different colors; different years are shown by points of different shapes. (B) Minus log10 p-values for the differences between lines (ANOVA) are shown, TAGs are ordered by p-value increase from top left to bottom right. Bonferroni adjusted 0.05 significance level is shown by red line; (C-G) abundances of five selected TAGs are shown across lines and years. Each point represents 1 sample, point shapes, and colors as in (A), lines show per-year averages. This figure complements main Fig. 3.

The primers sets used for HA89 (MAX1) genome reorganizations validation and gene expression analysis. *- has been also used in previous study (Makarenko et al., 2018). (DOCX 13 kb)

Background. Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, which often is associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. The CMS phenomenon investigations are also promote understanding of a fundamental issue of nuclear-cytoplasmic interactions in the ontogeny of higher plants. In the present study, we analyzed the structural changes in mitochondrial genomes of three alloplasmic lines of sunflower (Helianthus annuus). The investigation was focused on CMS line PET2, as there are very few reports about its mtDNA organization. Methods. The NGS sequencing, de novo assembly, and annotation of sunflower mitochondrial genomes were performed. The comparative analysis of mtDNA of HA89 fertile line and two HA89 CMS lines (PET1, PET2) occurred. Results. The mtDNA of the HA89 fertile line was almost identical to the HA412 line (NC_023337). The comparative analysis of HA89 fertile and CMS (PET1) analog mitochondrial genomes revealed 11852 bp inversion, 4732 bp insertion, 451 bp deletion and 18 variant sites. In mtDNA of HA89 (PET2) CMS line 77 kb translocation, 711 bp and 3780 bp deletions, as well as 1558 bp, 5050 bp, 14330 bp insertions were determined. There are also revealed 83 polymorphic sites sites in the PET2 mitochondrial genome, as compared with the fertile line Discussion. Among the revealed rearrangements the 1558 bp insertion resulted in new open reading frames formation - orf228 and orf246. The orf228 and orf246 could be the main reason for the development of PET2 CMS phenotype, whereas the role of other mtDNA reorganizations in CMS formation is negligible.

A master dissertation is devoted to the comparison of Russian second-generation migrants in two European capitals: Prague and Paris. It analyses ethnical identity of the children of Russian immigrants, existence of transnational ties with the homeland of their parents as well as their integration patterns. The study is based on in-depth interviews with the Russian second generation adolescents and their parents. The analytical part is preceded by a review of the main theoretical concepts used in the study with the focus on the existing theories of migrants' integration in general and second-generation integration in particular. Despite the fact that the results of the study can be generalised neither to the entire Europe, nor to the whole Czech Republic or France, the author tries to outline the main similarities and dissimilarities between the populations of The Russian secondgeneration immigrants in two European capitals as well as between two generations of Russian migrants and brings information about the Russian "new second generation" in general that can be used for further researches or policy-making.

The thesis focuses on knowledge of the Czech language as a factor that can contribute to the integration of Russian migrants to the Czech society. The relation of language skills on the whole and its partial sites with the other aspects of integration process according to different dimensions of integration is analyzed on the basis of a questionnaire survey among the members of Russian community living in Prague. The theoretical part is aimed at acquainting a reader with the problems of immigrant integration and providing an overview for the theoretical foundations for the empirical part.