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29. In vivo continuous estimation of H-dopamine synthesis and release in the cat caudate nucleus.

Author

Besson, M., Cheramy, A., Gauchy, C., and Glowinski, J.

Abstract

Synthesis and release of H-DA were examined during the continuous superfusion of a limited area of the ventricular surface of the cat caudate nucleus with l-3,5-H-tyrosine, using a cup technique. H−HO, an index of the conversion of l-3,5-H-tyrosine into H-Dopa, and H-DA were estimated in serial superfusate fractions. Alpha-methyl-paratyrosine (α-MpT) (10 M) inhibited rapidly and simultaneously both H−HO formation and H-DA release. Transection of the nigro-striatal dopaminergic pathway immediately blocked H-DA release but H−HO levels were reduced by 30% one hour later. [ABSTRACT FROM AUTHOR]

Published
1973
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30. Topographical distribution of substance P in the cat substantia nigra

Author

J. Glowinski, Bernard Kerdelhué, J.C. Beaujouan, M.J. Besson, R. Michelot, and Gauchy C

Subjects
Male, Brain Mapping, medicine.medical_specialty, Pars compacta, General Neuroscience, Cerebral peduncle, Substance P, Radioimmunoassay, Substantia nigra, Substantia Nigra, chemistry.chemical_compound, medicine.anatomical_structure, Endocrinology, chemistry, Internal medicine, Cats, medicine, Animals, Zona incerta, Female, Topographical distribution, Pars reticulata
Abstract

The substance P (SP) content in cat substantia nigra (SN) was estimated by radioimmunoassay in microdiscs punched on successive frontal sections. Only slight differences were seen in the regional distribution of SP within the SN. The highest content was found in the pars reticulata (20.7 pmol/mg protein) which was two times higher than in the pars compacta and pars lateralis. Identical results were found in the right and left part of the SN. Out of this area, as pedunculus cerebri and zona incerta, very low concentrations were detected.

Published
1979
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32. Chronic Methods to Study the Release of Catecholamines from the Neostriatum in Nonanaesthetized Monkeys (Macaca mulatta)1

Author

Bioulac, B., Cheramy, A., Besson, M.J., Rodriguez, F., Glowinski, J., Gauchy, C., and Vincent, J.D.

Abstract

The present report describes two methods allowing the chronic collection of catecholamine (CA) released from the neostriatum in the nonanaesthetized monkey ((Macaca mulatta).In both methods the monkeys were placed in a restraining chair. Indwelling electrodes allowed the correlation of behavioural observations with polygraphic recordings. The first method uses a special superfusing cup permanently implanted on the lateral surface of the caudate nucleus, which permits the demonstration of spontaneous and D‐amphetamine‐induced release of (3H)‐dopamine (DA) newly synthesized from L‐(3,5–3H)‐tyrosine over a period of six days. In the second method the collection of unlabelled CA is carried out by a localized ventricular perfusion. A radio‐enzymatic estimation enabled the measurement of unlabelled DA and noradrenaline. With this second method the animals were kept for 25 to 30 days.

Published
1979
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33. Distinct presynaptic control of dopamine release in striosomal and matrix areas of the cat caudate nucleus.

Author

Kemel, M L, Desban, M, Glowinski, J, and Gauchy, C

Abstract

By use of a sensitive in vitro microsuperfusion method, the cholinergic prsynaptic control of dopamine release was investigated in a prominent striosome (areas poor in acetylcholinesterase activity) located within the core of cat caudate nucleus and also in adjacent matrix area. The spontaneous release of [3H]dopamine continuously synthesized from [3H]tyrosine in the matrix area was found to be twice that in the striosomal area; the spontaneous and potassium-evoked releases of [3H]dopamine were calcium-dependent in both compartments. With 10(-6) M tetrodotoxin, 5 x 10(-5) M acetylcholine stimulated [3H]dopamine release in both striosomal and matrix areas, effects completely antagonized by atropine (10(-6) M), thus showing the involvement of muscarinic receptors located on dopaminergic nerve terminals. Experiments without tetrodotoxin revealed a more complex regulation of dopamine release in the matrix: (i) In contrast to results seen in the striosome, acetylcholine induced only a transient stimulatory effect on matrix dopamine release. (ii) Although 10(-6) M atropine completely abolished the cholinergic stimulatory effect on [3H]dopamine release in striosomal area, delayed and prolonged stimulation of [3H]dopamine release was seen with atropine in the matrix. The latter effect was completely abolished by the nicotinic antagonist pempidine (10(-5) M). Therefore, in the matrix, in addition to its direct (tetrodotoxin-insensitive) facilitatory action on [3H]dopamine release, acetylcholine exerts two indirect (tetrodotoxin-sensitive) opposing effects: an inhibition and a stimulation of [3H]dopamine release mediated by muscarinic and nicotinic receptors, respectively.

Published
1989
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35. In vivo continuous estimation of 3H-dopamine synthesis and release in the cat caudate nucleus

Author

Besson, M. J., Cheramy, A., Gauchy, C., and Glowinski, J.

Abstract

Synthesis and release of 3H-DA were examined during the continuous superfusion of a limited area of the ventricular surface of the cat caudate nucleus with l-3,5-3H-tyrosine, using a cup technique. 3H-H2O, an index of the conversion of l-3,5-3H-tyrosine into 3H-Dopa, and 2H-DA were estimated in serial superfusate fractions. Alpha-methyl-paratyrosine (a-MpT) (10-4 M) inhibited rapidly and simultaneously both 3H-H2O formation and 3H-DA release. Transection of the nigro-striatal dopaminergic pathway immediately blocked 3H-DA release but 3H-H2O levels were reduced by 30% one hour later.

Published
1973
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36. Chronic methods to study the release of catecholamines from the neostriatum in nonanaesthetized monkeys (Macaca mulatta)

Author

Cheramy A, Rodriquez F, Gauchy C, Vincent Jd, Besson Mj, Bioulac B, and Glowinski J

Subjects
Restraint, Physical, General Veterinary, Chemistry, Period (gene), Dopamine, Caudate nucleus, Anatomy, Haplorhini, Macaca mulatta, Corpus Striatum, Perfusion, Amphetamine, Norepinephrine, Catecholamines, Catecholamine, medicine, Methods, Animals, Macaca, Tyrosine, Animal Science and Zoology, Female, Caudate Nucleus, medicine.drug
Abstract

The present report describes two methods allowing the chronic collection of catecholamine (CA) released from the neostriatum in the nonanaesthetized monkey (Macaca mulatta). In both methods the monkeys were placed in a restraining chair. Indwelling electrodes allowed the correlation of behavioural observations with polygraphic recordings. The first method uses a special superfusing cup permanently implanted on the lateral surface of the caudate nucleus, which permits the demonstration of spontaneous and D-amphetamine-induced release of (3H)-dopamine (DA) newly synthesized from L-(3,5-3H)-tyrosine over a period of six days. In the second method the collection of unlabelled CA is carried out by a localized ventricular perfusion. A radio-enzymatic estimation enabled the measurement of unlabelled DA and noradrenaline. With this second method the animals were kept for 25 to 30 days.

Published
1979

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