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Submitted by Igor Renan Honorato Gatto (igor.gatto@unesp.br) on 2019-01-17T23:23:58Z No. of bitstreams: 1 Tese_Gatto IRH_2018.pdf: 2351393 bytes, checksum: 8996a48d44337e62a338fce6256190d8 (MD5) Approved for entry into archive by Tatiana Camila Gricio (tatiana.gricio@unesp.br) on 2019-01-18T11:05:28Z (GMT) No. of bitstreams: 1 gatto_irh_dr_jabo.pdf: 2351393 bytes, checksum: 8996a48d44337e62a338fce6256190d8 (MD5) Made available in DSpace on 2019-01-18T11:05:28Z (GMT). No. of bitstreams: 1 gatto_irh_dr_jabo.pdf: 2351393 bytes, checksum: 8996a48d44337e62a338fce6256190d8 (MD5) Previous issue date: 2018-12-07 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O gênero Pestivirus é composto por agentes geneticamente distintos, responsáveis por infecções em suínos, bovinos, ovinos, caprinos e outros animais domésticos e silvestres. O atypical porcine pestivirus (APPV), é um pestivirus geneticamente atípico recentemente identificado e associado a casos de tremor congênito (CT) em leitões recém-nascidos em quatro continentes, sugerindo uma distribuição mundial do APPV. Assim, os objetivos deste estudo foram: 1) detectar a presença do APPV em sêmen, líquidos prepuciais e swabs prepuciais de cachaços adultos por reação em cadeia da polimerase via transcriptase reversa quantitativa (qRT-PCR), 2) caracterizar geneticamente um subgrupo de amostras positivas de sêmen para melhor entender a ecologia do APPV em cachaços comerciais e o risco potencial de transmissão do APPV via sêmen, 3) investigar a presença do APPV em leitões com CT no Brasil e (4) caracterizar geneticamente um subconjunto de amostras do APPV oriundas de leitões. Um total de 597 amostras de sêmen, fluido prepucial e swabs prepuciais cada amostra representando um cachaço diferente foram obtidas de quatro granjas comerciais (sêmen) localizadas em três estados diferentes nos Estados Unidos e 13 leitões com CT de quatro granjas em dois estados do Brasil foram amostrados. RNA viral foi detectado por qRT-PCR em 15,08% das amostras (90/597), sendo o maior percentual 23,81% de positivo em swabs prepuciais (5/21) seguido de 22,81% no fluído prepucial (26/114) e de 12,91% no sêmen (59/457). Com base na análise filogenética do gene Npro do APPV, diferentes estirpes virais circulavam na mesma granja. O RNA viral foi detectado por qRT-PCR no cerebelo ou cerebelo e medula espinhal em 100% dos leitões com CT, e o APPV não foi detectado em nenhuma amostra de tecido de leitões clinicamente não afetados. Um subconjunto de amostras positivas foi selecionado para o sequenciamento de parte do gene NS3. A análise filogenética revelou que as sequências brasileiras do gene NS3 formaram um cluster independente e tiveram a maior identidade de sequência de nucleotídeos com uma amostra dos Estados Unidos. Este é o primeiro relato de detecção do APPV em sêmen de cachaços comerciais e a primeira identificação da infecção por APPV em leitões com CT na América do Sul. The genus Pestivirus is composed of genetically distinct viruses, responsible for infections in swine, cattle, sheep, goats and other domestic and wild animals. The atypical porcine pestivirus (APPV) is a genetically atypical pestivirus recently identified and associated with cases of congenital tremor (CT) in newborn piglets from four continents, suggesting a worldwide distribution of the APPV. Accordingly, the objectives of this study were to 1) detect the presence of APPV in semen, preputial fluids and preputial swabs from adult boars by quantitative reverse transcription PCR (qRT-PCR), 2) genetically characterize a subset of positive semen samples to better understand the ecology of APPV in commercial boar studs and the potential risk of transmission of APPV via semen, 3) investigate the presence of APPV in piglets with CT in Brazil and (4) genetically characterize a subset of APPV samples from CT-piglets. A total of 597 samples of semen, preputial fluid and preputial swabs each samples representing a different boar were obtained from four commercial boar studs located in three different states in the United States and 13 CT-piglets from four farms in two different states in Brazil were sampled. Viral RNA was detected by qRT-PCR in 90 samples (15.08%; 90/597), with the greatest per cent positive from preputial swabs (23.81%; 5/21) followed by preputial fluid (22.81%; 26/114) and semen (12.91%; 59/457). Based on phylogenetic analysis of the APPV Npro gene, different viral strains can be on the same farm at the same and different times. Viral RNA was detected by qRT-PCR in the cerebellum or cerebellum and spinal cord in the 100% of the piglets with CT, and APPV was not detected in any tissue sample from clinically non-affected piglets. A subset of positive samples was selected for sequencing of part of the NS3 gene. Phylogenetic analysis revealed that Brazilian sequences of the NS3 gene formed an independent cluster and had the highest nucleotide sequence identity with a sample from the United States. This is the first report of detection of APPV in semen from commercial boar studs and the first identification of APPV infection in piglets with CT in South America. 001

Made available in DSpace on 2016-08-24T12:43:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015. Added 1 bitstream(s) on 2016-08-24T13:16:19Z : No. of bitstreams: 1 ISSN1679-4605-2015-11-02-170-176.pdf: 380493 bytes, checksum: 51a310c8e735c9e2a590672f28579fdc (MD5) Tendo em vista que o princípio de Educação em Saúde é o repasse de informações à comunidade, e levando-se em conta a importante colaboração que as crianças oferecem como multiplicadores de conhecimento à sociedade, o projeto “Natal do EducaPrev” teve como objetivo a integração dos alunos de pós-graduação do Departamento de Medicina Veterinária Preventiva e Reprodução Animal da FCAV/Unesp/Jaboticabal/SP, com os crianças de um abrigo do município, para a realização de atividades educativas que possibilitaram a conscientização das crianças sobre temas importantes como guarda responsável de animais, leptospirose e raiva. O projeto foi um sucesso, tendo resultado em uma ótima socialização entre os envolvidos e atingindo o intuito de transferência de informações, além da possibilidade do cumprimento da tríade indissociável “Pesquisa; Ensino e Extensão”, que nunca deve ser desvinculada dentro dos princípios básicos da Universidade. Given that the main purpose of health education is to transfer information to the population, and considering the important role that children play as knowledge multipliers in society, the objective of the “EducaPrev Christmas” project was to promote the interaction of postgraduate students from the Department of Preventive Veterinary Medicine and Animal Reproduction of FCAV/UNESP, Jaboticabal campus, with the children of the “Lar Caminho” municipal shelter. Educational activities were undertaken to raise the awareness of the children concerning themes such as responsible ownership, leptospirosis, and rabies. The project was successful, resulting in good interaction among the participants and fulfilling the main objective of information transfer. In addition, it was an opportunity to implement the inseparable triad of “research, education, and extension” that should never be ignored as one of the basic principles of the university. Teniendo en cuenta que el principio de Educación para la Salud es la transmisión de información a la comunidad, y teniendo en cuenta la importante contribución que los niños ofrecen como multiplicadores de la sociedad del conocimiento, el proyecto "Navidad del EducaPrev" tuvo como objetivo la integración de los estudiantes de posgrado del Departamento de Medicina Veterinaria Preventiva y Reproducción Animal FCAV / UNESP / Jaboticabal / SP, con niños menores de un municipio, para llevar a cabo actividades educativas que permitieron a la concientización de los niños sobre temas importantes como el cuidado responsable con los animales, la leptospirosis y la rabia. El proyecto fue un éxito y dió lugar a una gran socialización entre los interesados y alcanzó el objetivo de la transferencia de información, más allá de la posibilidad de cumplir tríada inseparable "Investigación, Educación y Extensión", que nunca debe ser separada dentro de los principios básicos de la Universidad. Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal

Made available in DSpace on 2018-12-11T17:03:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01. Added 1 bitstream(s) on 2019-10-09T18:30:28Z : No. of bitstreams: 1 S0103-84782016000901607.pdf: 293033 bytes, checksum: 9e3226bef63f001928bf6a5cbda0acb6 (MD5) This study investigated the occurrence of antibodies for BVDV-1 in swine herds located in the region of Mossoró city of the state of Rio Grande do Norte, Brazil. A sample size of 412 animals was estimated assuming unknown prevalence (set at 50%). Virus neutralization assay was used to the detect the presence of antibodies for BVDV-1 and the results found were analysed using multivariable logistic regression model. The obtained prevalence was 4% at animal level and 45% at the animal and herd level. The titers were highly variable between animals and within farms. The multivariable logistic regression analysis showed an association between being housed outside and exposure to BVDV-1 (OR=0.24, 95% CI:0.06, 0.96, P=0.04). Highly correlated data and low prevalence of antibodies at the animal level resulted in insufficient power to detect significant differences with other selected risk factors. In conclusion, the prevalence is within the range reported for other countries. Departamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP) Departamento de Medicina Populacional Universidade de Guelph Universidade Federal Rural do Semi-Árido (UFERSA) Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária (FMVA) Universidade Estadual Paulista (UNESP) Instituto Biológico (IB/APTA/SAA-SP) Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP) Departamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP) Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária (FMVA) Universidade Estadual Paulista (UNESP) Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP)

Made available in DSpace on 2022-04-28T19:03:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01 Strangles is a disease of the respiratory tract of horses caused by Streptococcus equi. The disease has high morbidity and low lethality, and produces economic losses due to low performance and high cost treatment. This research focused on evaluate antibiotic resistance of Streptococcus equi from the nasal cavity of healthy horses from the region Fernandópolis - São Paulo. Fifty (50) mongrel healthy horses, ageing from twelve to twenty four (12-24) months were used. Samples from the nasal cavity of each animal were obtained using sterile swabs and after were cultured on blood agar base plus 5[%] defibrinated sheep blood. Plates that showed mucous colonies and ß - hemolytic were tested using Gram stain method, the catalase test, and identified by the system API Rapid ID thirty-two 32 Strep. All isolates were analyzed by plate difusion. Sixty seven of the obtained isolates were Streptococcus equi subspecies equi and zooepidemicus. There was higher prevalence of S. subsp equi zooepidemicus (p < 0.001). Most isolates of S. equi subsp equi were antibiotical multiresistant with significant differences between different antibiotics (p < 0.005), whereas higher susceptibility was observed in isolated S. subsp equi zooepidemicus (p < 0.05). The results showed that both subspecies of Streptococcus equi are part of the normal flora of the nasal cavity of horses, and that they differ in susceptibility to antibiotics. Médico Veterinário auto nomo Departamento de Medicina Veterinária Preventiva Universidade Estadual Paulista Universidade Camilo Castelo Branco (UNICASTELO) Departamento de Medicina Veterinária Preventiva Universidade Estadual Paulista

Made available in DSpace on 2015-08-20T17:09:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-26. Added 1 bitstream(s) on 2015-08-20T17:26:20Z : No. of bitstreams: 1 000837037_20160328.pdf: 135986 bytes, checksum: b392bdf3a2b1bf28c5b65d4d51e4a4a9 (MD5) Bitstreams deleted on 2016-03-28T13:37:42Z: 000837037_20160328.pdf,. Added 1 bitstream(s) on 2016-03-28T13:38:46Z : No. of bitstreams: 1 000837037.pdf: 3762653 bytes, checksum: bea2b617ceb4163e2c7bb4b39bcc4f39 (MD5) Os suínos são susceptíveis a infecção por diversas espécies de Pestivirus, a infecção de suínos com pestivírus de ruminantes tem gerado preocupação em diversos países, dependendo do método utilizado, o diagnóstico entre BVDV e CSFV pode ser dificultado. O presente trabalho teve como objetivo verificar a prevalência de anticorpos contra o vírus da diarreia viral bovina (BVDV) em suínos terminados. Para tal, foram investigadas as prevalências do BVDV-1 e 2, obtiveram-se informações de prevalência de acordo com a origem das amostras, foram associados fatores de risco à ocorrência da infecção, verificado as diferenças entre os títulos das amostras positivas testadas com 100 TCID50 e 50 TCID50 e realizado diagnóstico diferencial para PSC. Os soros sanguíneo foram obtidos de suínos enviados para matadouros-frigorífico no estado de São Paulo. As propriedades de origem dos suínos eram localizadas nos estados do Mato Grosso, Mato Grosso do Sul, Goiás, São Paulo, Paraná, Santa Catarina e Rio Grande do Sul. As amostras foram submetidas ao teste de VN conforme preconizado pelo Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (OIE), para às sororreagentes, foi realizado o diagnóstico diferencial para PSC pelo método de ELISA. Foram analisadas 1705 amostras de soro sanguíneo de suínos, obtidas de 33 propriedades. O teste de VN indicou 5,34% (91/1705) de amostras sororreagentes ao BVDV, sendo 3,0% (51/1705) para o genótipo 1 e 2,35% (40/1705) para o genótipo 2. Considerando a origem das amostras, destacam-se os estados do Mato Grosso, com 10,00% e Rio Grande do Sul, com 9,18% de amostras sororreagentes ao BVDV-1, contra o BVDV-2, destacam-se os estados do Mato Grosso, com 6,0% e Mato Grosso do Sul, com 4,29% de sororreagentes. Nenhum fator de risco teve associação significativa com a ocorrência de infecção ao BVDV-1, contudo, para o BVDV-2, foram encontradas associações significativas... Several species of Pestivirus have pigs as susceptible hosts. The infection of ruminant pestivirus in pigs have been concerning many countries and depending on the diagnosis test used it is hard to differ BVDV from CSFV infections due to serological cross-reaction in diagnostic tests. This study aimed on determining the prevalence of antibodies against bovine viral diarrhea virus (BVDV) in finisher pigs. Prevalence of BVDV -1 and BVDV-2 was investigated and information about the samples origin were analyzed in order to associate the the occurrence of the infection with possible risk factors. The samples were tested using 100 TCID50 and 50 TCID50 and the differences between the titers were analyzed. In addition, differential diagnosis for Classical Swine Fever (CSF) was done in all positive samples. The blood sera samples were obtained from pigs sent to slaughterhouses in the state of São Paulo. The origin of the animals was from farms located in the states of Mato Grosso, Mato Grosso do Sul, Goias, Sao Paulo, Parana, Santa Catarina and Rio Grande do Sul. The samples were tested using the virus neutralization (VN) test according to the Manual of Diagnostic Tests Vaccines for Terrestrial Animals (OIE) and the positive samples were ELISA tested to differ from CSF. 1705 swine serum samples from 33 farms were tested. The VN test showed 5.34% (91 / 1705) of seropositive samples for BVDV, with 3.0% (51 / 1705) for genotype 1 and 2.35% (40 / 1705) for genotype 2. Considering the origin of the samples, the highlights are the states of Mato Grosso, with 10.00% and Rio Grande do Sul, with 9.18% of seropositive for BVDV-1. Regarding to BVDV-2, states that had the highest number of positive samples were Mato Grosso (6.0%) and Mato Grosso do Sul, (4.29%). No significant association was found between risk factors the occurrence of BVDV-1 infection, however, for BVDV-2 positive cases significant associations were found with the risk factors ...