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3. Microbial antioxidant defense enzymes

Author

Staerck, Cindy, Gastebois, Amandine, Vandeputte, Patrick, Calenda, Alphonse, Larcher, Gérald, Gillmann, Louiza, Papon, Nicolas, Bouchara, Jean-Philippe, and Fleury, Maxime J.J.

Published
2017
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5. Deciphering the Role of PIG1 and DHN-Melanin in Scedosporium apiospermum Conidia

Author

Guegan, Hélène, primary, Poirier, Wilfried, additional, Ravenel, Kevin, additional, Dion, Sarah, additional, Delabarre, Aymeric, additional, Desvillechabrol, Dimitri, additional, Pinson, Xavier, additional, Sergent, Odile, additional, Gallais, Isabelle, additional, Gangneux, Jean-Pierre, additional, Giraud, Sandrine, additional, and Gastebois, Amandine, additional

Published
2023
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10. Draft Genome Sequence of the Environmental Fungus Scedosporium dehoogii

Author

Shiller, Jason, primary, Duvaux, Ludovic, additional, Vandeputte, Patrick, additional, Lemaire, Christophe, additional, Rougeron, Amandine, additional, De Bernonville, Thomas Dugé, additional, Giraud, Sandrine, additional, Le Cam, Bruno, additional, Bouchara, Jean-Philippe, additional, and Gastebois, Amandine, additional

Published
2021
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11. The Glycosylphosphatidylinositol-Anchored Superoxide Dismutase of Scedosporium apiospermum Protects the Conidia from Oxidative Stress

Author

Staerck, Cindy, primary, Yaakoub, Hajar, additional, Vandeputte, Patrick, additional, Tabiasco, Julie, additional, Godon, Charlotte, additional, Gastebois, Amandine, additional, Giraud, Sandrine, additional, Guillemette, Thomas, additional, Calenda, Alphonse, additional, Delneste, Yves, additional, Fleury, Maxime, additional, and Bouchara, Jean-Philippe, additional

Published
2021
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14. Diversity and Evolution of Sensor Histidine Kinases in Eukaryotes

Author

Kabbara, Samar, Herivaux, Anais, Dugé de Bernonville, Thomas, Courdavault, Vincent, Clastre, Marc, Gastebois, Amandine, Osman, Marwan, Hamzé, Monzer, Cock, J Mark, Schaap, Pauline, Papon, Nicolas, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours (UT), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre AZM pour la Recherche en Biotechnologie et ses Applications, Université Libanaise, Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), School of Life Sciences, University of Dundee, Gestionnaire, HAL Sorbonne Université 5, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Université de Tours

Subjects
Evolution, Molecular, eukaryotes, Histidine Kinase, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], phylogenetic analysis, histidine kinases, two-component systems, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Eukaryota, cell signaling, Phylogeny, Signal Transduction, Research Article
Abstract

International audience; Histidine kinases (HKs) are primary sensor proteins that act in cell signaling pathways generically referred to as “two-component systems” (TCSs). TCSs are among the most widely distributed transduction systems used by both prokaryotic and eukaryotic organisms to detect and respond to a broad range of environmental cues. The structure and distribution of HK proteins are now well documented in prokaryotes, but information is still fragmentary for eukaryotes. Here, we have taken advantage of recent genomic resources to explore the structural diversity and the phylogenetic distribution of HKs in the prominent eukaryotic supergroups. Searches of the genomes of 67 eukaryotic species spread evenly throughout the phylogenetic tree of life identified 748 predicted HK proteins. Independent phylogenetic analyses of predicted HK proteins were carried out for each of the major eukaryotic supergroups. This allowed most of the compiled sequences to be categorized into previously described HK groups. Beyond the phylogenetic analysis of eukaryotic HKs, this study revealed some interesting findings: 1) characterization of some previously undescribed eukaryotic HK groups with predicted functions putatively related to physiological traits; 2) discovery of HK groups that were previously believed to be restricted to a single kingdom in additional supergroups, and 3) indications that some evolutionary paths have led to the appearance, transfer, duplication, and loss of HK genes in some phylogenetic lineages. This study provides an unprecedented overview of the structure and distribution of HKs in the Eukaryota and represents a first step toward deciphering the evolution of TCS signaling in living organisms.

Published
2018
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15. Draft Genome Sequence of the Human-Pathogenic Fungus Scedosporium boydii

Author

Duvaux, Ludovic, Shiller, Jason, Vandeputte, Patrick, Duge de Bernonville, Thomas, Thornton, Christopher, Papon, Nicolas, Le Cam, Bruno, Bouchara, Jean-Philippe, Gastebois, Amandine, Génétique Diversité et Ecophysiologie des Céréales - Clermont Auvergne (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Groupe d'Etude des Interactions Hôte-Parasite (GEIHP), Université d'Angers (UA), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lausanne (UNIL), Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK, Groupe d’Etude des Interactions Hôte-Pathogène, Génétique et Horticulture (GenHort), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours (UT), Biosciences, College of Life and Environmental Sciences, University of Exeter, Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Université de Lausanne = University of Lausanne (UNIL)

Subjects
Eukaryotes, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], skin and connective tissue diseases, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

The opportunistic fungal pathogen Scedosporium boydii is the most common Scedosporium species in French patients with cystic fibrosis. Here we present the first genome report for S. boydii, providing a resource which may enable the elucidation of the pathogenic mechanisms in this species.

Published
2017
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16. Transcriptrice Insight in the Control of Legume Root Secondary Infection by the Sinorhizobium meliloti Transcriptional Regulator Clr

Author

Zou, Lan, Gastebois, Amandine, Mathieu-Demazière, Céline, Sorroche, Fernando, Masson-Boivin, Catherine, Batut, Jacques, Garnerone, Anne-Marie, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Groupe d’Etude des Interactions Hôte-Pathogène, Université d'Angers (UA), Groupe d'Etude des Interactions Hôte-Parasite (GEIHP), Laboratoire de Chimie Agro-Industrielle (LCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Toulouse, Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Chimie Agro-Industrielle (CAI), Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole nationale supérieure des ingénieurs en arts chimiques et technologiques-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017
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17. The Identification of Phytohormone Receptor Homologs in Early Diverging Fungi Suggests a Role for Plant Sensing in Land Colonization by Fungi

Author

Herivaux, Anais, de Bernonville, Thomas Duge, Roux, Christophe, Clastre, Marc, Courdavault, Vincent, Gastebois, Amandine, Bouchara, Jean-Philippe, James, Timothy Y., Latge, Jean-Paul, Martin, Francis, Papon, Nicolas, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours (UT), Interactions Microbiennes dans la Rhizosphère et les Racines, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT), Department of Ecology and Evolutionary Biology (Faculty of Biology), University of Science-Vietnam National Universities, Aspergillus, Institut Pasteur [Paris] (IP), Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratory of Excellence Advanced Research on the Biology of Tree and Forest Ecosystems (ARBRE) [ANR 11 LABX 0002 01], U.S. DOE through Oak Ridge National Laboratory Scientific Focus Area for Genomics Foundational Sciences (Plant Microbe Interfaces Project), John W. Taylor, ANR-11-LABX-0002,ARBRE,Recherches Avancées sur l'Arbre et les Ecosytèmes Forestiers(2011), Groupe d’Etude des Interactions Hôte-Pathogène, Université de Tours, Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées, Institut Pasteur [Paris], Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Receptors, Cell Surface/genetics, Histidine Kinase, [SDV]Life Sciences [q-bio], fungi, Fungi, food and beverages, Computational Biology, MESH: Histidine Kinase/genetics, Receptors, Cell Surface, Opinion/Hypothesis, Plants, Microbiology, QR1-502, Evolution, Molecular, Plant Growth Regulators, MESH: Plants/chemistry, MESH: Evolution, Molecular, MESH: Plant Growth Regulators/metabolism, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Computational Biology, MESH: Fungi/genetics
Abstract

International audience; Histidine kinases (HKs) are among the most prominent sensing proteins studied in the kingdom Fungi. Their distribution and biological functions in early diverging fungi (EDF), however, remain elusive. We have taken advantage of recent genomic resources to elucidate whether relationships between the occurrence of specific HKs in some EDF and their respective habitat/lifestyle could be established. This led to the unexpected discovery of fungal HKs that share a high degree of similarity with receptors for plant hormones (ethylene and cytokinin). Importantly, these phytohormone receptor homologs are found not only in EDF that behave as plant root symbionts or endophytes but also in EDF species that colonize decaying plant material. We hypothesize that these particular sensing proteins promoted the interaction of EDF with plants, leading to the conquest of land by these ancestral fungi.

Published
2017
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19. Diversity and Evolution of Sensor Histidine Kinases in Eukaryotes.

Author

Kabbara, Samar, Hérivaux, Anaïs, Bernonville, Thomas Dugé de, Courdavault, Vincent, Clastre, Marc, Gastebois, Amandine, Osman, Marwan, Hamze, Monzer, Cock, J Mark, Schaap, Pauline, and Papon, Nicolas

Subjects
HISTIDINE kinases, PROKARYOTES, EUKARYOTES, EUKARYOTIC genomes, DETECTORS, BIOLOGICAL evolution, GENETIC transduction
Abstract

Histidine kinases (HKs) are primary sensor proteins that act in cell signaling pathways generically referred to as "two-component systems" (TCSs). TCSs are among the most widely distributed transduction systems used by both prokaryotic and eukaryotic organisms to detect and respond to a broad range of environmental cues. The structure and distribution of HK proteins are now well documented in prokaryotes, but information is still fragmentary for eukaryotes. Here, we have taken advantage of recent genomic resources to explore the structural diversity and the phylogenetic distribution of HKs in the prominent eukaryotic supergroups. Searches of the genomes of 67 eukaryotic species spread evenly throughout the phylogenetic tree of life identified 748 predicted HK proteins. Independent phylogenetic analyses of predicted HK proteins were carried out for each of the major eukaryotic supergroups. This allowed most of the compiled sequences to be categorized into previously described HK groups. Beyond the phylogenetic analysis of eukaryotic HKs, this study revealed some interesting findings: 1) characterization of some previously undescribed eukaryotic HK groups with predicted functions putatively related to physiological traits; 2) discovery of HK groups that were previously believed to be restricted to a single kingdom in additional supergroups, and 3) indications that some evolutionary paths have led to the appearance, transfer, duplication, and loss of HK genes in some phylogenetic lineages. This study provides an unprecedented overview of the structure and distribution of HKs in the Eukaryota and represents a first step toward deciphering the evolution of TCS signaling in living organisms. [ABSTRACT FROM AUTHOR]

Published
2019
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21. Developing collaborative works for faster progress on fungal respiratory infections in cystic fibrosis.

Author

Schwarz, Carsten, Vandeputte, Patrick, Rougeron, Amandine, Giraud, Sandrine, de Bernonville, Thomas Dugé, Duvaux, Ludovic, Gastebois, Amandine, Alastruey-Izquierdo, Ana, Teresa Martín-Gomez, Maria, Mazuelos, Estrella Martin, Sole, Amparo, Cano, Josep, Pemán, Javier, Quindos, Guillermo, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Chen, Sharon, Delhaés, Laurence, Favennec, Loïc, and Ranque, Stéphane

Abstract

Cystic fibrosis (CF) is the major genetic inherited disease in Caucasian populations. The respiratory tract of CF patients displays a sticky viscous mucus, which allows for the entrapment of airborne bacteria and fungal spores and provides a suitable environment for growth of microorganisms, including numerous yeast and filamentous fungal species. As a consequence, respiratory infections are the major cause of morbidity and mortality in this clinical context. Although bacteria remain the most common agents of these infections, fungal respiratory infections have emerged as an important cause of disease. Therefore, the International Society for Human and Animal Mycology (ISHAM) has launched a working group on Fungal respiratory infections in Cystic Fibrosis (Fri-CF) in October 2006, which was subsequently approved by the European Confederation of Medical Mycology (ECMM). Meetings of this working group, comprising both clinicians and mycologists involved in the follow-up of CF patients, as well as basic scientists interested in the fungal species involved, provided the opportunity to initiate collaborative works aimed to improve our knowledge on these infections to assist clinicians in patient management. The current review highlights the outcomes of some of these collaborative works in clinical surveillance, pathogenesis and treatment, giving special emphasis to standardization of culture procedures, improvement of species identificationmethods including the development of nonculture-based diagnostic methods, microbiome studies and identification of new biological markers, and the description of genotyping studies aiming to differentiate transient carriage and chronic colonization of the airways. The review also reports on the breakthrough in sequencing the genomes of the main Scedosporium species as basis for a better understanding of the pathogenic mechanisms of these fungi, and discusses treatment options of infections caused by multidrug resistant microorganisms, such as Scedosporium and Lomentospora species and members of the Rasamsonia argillacea species complex. [ABSTRACT FROM AUTHOR]

Published
2018
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22. β(1-3)Glucanosyltransferase Gel4p Is Essential for Aspergillus fumigatus ▿

Author

Gastebois, Amandine, Fontaine, Thierry, Latgé, Jean-Paul, Mouyna, Isabelle, Latge, J.-P., Aspergillus, Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris]

Subjects
Glycosylphosphatidylinositols, Glycosylphosphatidylinositol, Genes, Fungal, Biology, Microbiology, Gene Expression Regulation, Enzymologic, Aspergillus fumigatus, Pathogenesis, Fungal Proteins, 03 medical and health sciences, chemistry.chemical_compound, Phylogenetics, Gene Expression Regulation, Fungal, MESH: Phylogeny, Molecular Biology, Gene, Mycelium, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Phylogeny, 030304 developmental biology, MESH: Genes, Essential, 0303 health sciences, Fungal protein, Genes, Essential, 030306 microbiology, MESH: Gene Expression Regulation, Enzymologic, Glucan Endo-1,3-beta-D-Glucosidase, General Medicine, Articles, Gene deletion, biology.organism_classification, MESH: Mycelium, Chromatography, Ion Exchange, MESH: Chromatography, Ion Exchange, MESH: Glucan Endo-1,3-beta-D-Glucosidase, MESH: Glycosylphosphatidylinositols, chemistry, MESH: Gene Deletion, MESH: Fungal Proteins, MESH: Aspergillus fumigatus, MESH: Genes, Fungal, MESH: Gene Expression Regulation, Fungal, Gene Deletion
Abstract

The β(1-3)glucanosyltransferase GEL family of Aspergillus fumigatus contains 7 genes, among which only 3 are expressed during mycelial growth. The role of the GEL4 gene was investigated in this study. Like the other Gelps, it encodes a glycosylphosphatidylinositol (GPI)-anchored protein. In contrast to the other β(1-3)glucanosyltransferases analyzed to date, it is essential for this fungal species.

Published
2010
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24. Transcriptomic Insight in the Control of Legume Root Secondary Infection by the Sinorhizobium meliloti Transcriptional Regulator Clr.

Author

Lan Zou, Gastebois, Amandine, Mathieu-Demazière, Céline, Sorroche, Fernando, Masson-Boivin, Catherine, Batut, Jacques, and Garnerone, Anne-Marie

Subjects
LEGUME diseases & pests, ORTHOPTERA, GENE regulatory networks
Abstract

The cAMP-dependent transcriptional regulator Clr of Sinorhizobium meliloti regulates the overall number of infection events on Medicago roots by a so-far unknown mechanism requiring smc02178, a Clr-target gene of unknown function. In order to shed light on the mode of action of Clr on infection and potentially reveal additional biological functions for Clr, we inventoried genomic Clr target genes by transcriptome profiling. We have found that Clr positively controls the synthesis of cAMP-dependent succinoglycan as well as the expression of genes involved in the synthesis of a so-far unknown polysaccharide compound. In addition, Clr activated expression of 24 genes of unknown function in addition to smc02178. Genes negatively controlled by Clr were mainly involved in swimming motility and chemotaxis. Functional characterization of two novel Clr-activated genes of unknown function, smb20495 and smc02177, showed that their expression was activated by the same plant signal as smc02178 ex planta. In planta, however, symbiotic expression of smc02177 proved independent of clr. Both smc02177 and smb20495 genes were strictly required for the control of secondary infection on M. sativa. None of the three smc02177, smc02178 and smb20495 genes were needed for plant signal perception. Altogether this work provides a refined view of the cAMP-dependent Clr regulon of S. meliloti. We specifically discuss the possible roles of smc02177, smc02178, smb20495 genes and other Clr-controlled genes in the control of secondary infection of Medicago roots. [ABSTRACT FROM AUTHOR]

Published
2017
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27. Draft Genome Sequence of the Human-Pathogenic Fungus Scedosporium boydii .

Author

Duvaux L, Shiller J, Vandeputte P, Dugé de Bernonville T, Thornton C, Papon N, Le Cam B, Bouchara JP, and Gastebois A

Abstract

The opportunistic fungal pathogen Scedosporium boydii is the most common Scedosporium species in French patients with cystic fibrosis. Here we present the first genome report for S. boydii , providing a resource which may enable the elucidation of the pathogenic mechanisms in this species., (Copyright © 2017 Duvaux et al.)

Published
2017
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28. Transcriptomic Insight in the Control of Legume Root Secondary Infection by the Sinorhizobium meliloti Transcriptional Regulator Clr.

Author

Zou L, Gastebois A, Mathieu-Demazière C, Sorroche F, Masson-Boivin C, Batut J, and Garnerone AM

Abstract

The cAMP-dependent transcriptional regulator Clr of Sinorhizobium meliloti regulates the overall number of infection events on Medicago roots by a so-far unknown mechanism requiring smc02178 , a Clr-target gene of unknown function. In order to shed light on the mode of action of Clr on infection and potentially reveal additional biological functions for Clr, we inventoried genomic Clr target genes by transcriptome profiling. We have found that Clr positively controls the synthesis of cAMP-dependent succinoglycan as well as the expression of genes involved in the synthesis of a so-far unknown polysaccharide compound. In addition, Clr activated expression of 24 genes of unknown function in addition to smc02178 . Genes negatively controlled by Clr were mainly involved in swimming motility and chemotaxis. Functional characterization of two novel Clr-activated genes of unknown function, smb20495 and smc02177 , showed that their expression was activated by the same plant signal as smc02178 ex planta . In planta , however, symbiotic expression of smc02177 proved independent of clr. Both smc02177 and smb20495 genes were strictly required for the control of secondary infection on M. sativa . None of the three smc02177, smc02178 and smb20495 genes were needed for plant signal perception. Altogether this work provides a refined view of the cAMP-dependent Clr regulon of S. meliloti . We specifically discuss the possible roles of smc02177, smc02178, smb20495 genes and other Clr-controlled genes in the control of secondary infection of Medicago roots.

Published
2017
Full Text
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29. beta(1-3)Glucanosyltransferase Gel4p is essential for Aspergillus fumigatus.

Author

Gastebois A, Fontaine T, Latgé JP, and Mouyna I

Subjects
Aspergillus fumigatus genetics, Aspergillus fumigatus growth & development, Chromatography, Ion Exchange, Fungal Proteins genetics, Fungal Proteins metabolism, Gene Deletion, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Genes, Fungal genetics, Glucan Endo-1,3-beta-D-Glucosidase genetics, Glycosylphosphatidylinositols metabolism, Mycelium enzymology, Mycelium genetics, Mycelium growth & development, Phylogeny, Aspergillus fumigatus enzymology, Genes, Essential genetics, Glucan Endo-1,3-beta-D-Glucosidase metabolism
Abstract

The beta(1-3)glucanosyltransferase GEL family of Aspergillus fumigatus contains 7 genes, among which only 3 are expressed during mycelial growth. The role of the GEL4 gene was investigated in this study. Like the other Gelps, it encodes a glycosylphosphatidylinositol (GPI)-anchored protein. In contrast to the other beta(1-3)glucanosyltransferases analyzed to date, it is essential for this fungal species.

Published
2010
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