21 results on '"Garzetti D"'
Search Results
2. Microbiota Dysbiosis Controls the Neuroinflammatory Response after Stroke
- Author
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Singh, V., primary, Roth, S., additional, Llovera, G., additional, Sadler, R., additional, Garzetti, D., additional, Stecher, B., additional, Dichgans, M., additional, and Liesz, A., additional
- Published
- 2016
- Full Text
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3. Different siderophores contribute to the high-pathogenicity phenotype in Yersinia
- Author
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Rakin, A., primary and Garzetti, D., additional
- Published
- 2013
- Full Text
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4. Tracing genomic variations in two highly virulent Yersinia enterocolitica strains with unequal ability to compete for host colonization
- Author
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Garzetti Debora, Bouabe Hicham, Heesemann Juergen, and Rakin Alexander
- Subjects
Yersinia enterocolitica ,Hyper-virulent ,Genome comparison ,Diversity ,Host colonization ,Virulence factors ,YscP ,YopM ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Yersinia enterocolitica is a gastrointestinal foodborne pathogen found worldwide and which especially affects infants and young children. While different bioserotypes have been associated with varying pathogenicity, research on Y. enterocolitica is mainly conducted on the highly virulent mouse-lethal strains of biotype 1B and serotype O:8. We demonstrate here that two Y. enterocolitica bioserotype 1B/O:8 strains, 8081 and WA-314, display different virulence and fitness properties in a mouse model. In vivo co-infection experiments revealed that strain WA-314 overcomes strain 8081 in the colonization of spleen and liver. To trace the reasons of this incongruity, we present here the first high-quality sequence of the whole genome of strain WA-314 and compare it to the published genome of strain 8081. Results Regions previously accepted as unique to strain 8081, like the YAPI and YGI-3 genomic islands, are absent from strain WA-314, confirming their strain-specificity. On the other hand, some fitness- and bacterial competition-associated features, such as a putative colicin cluster and a xenobiotic-acyltransferase-encoding gene, are unique to strain WA-314. Additional acquisitions of strain WA-314 are seven prophage-like regions. One of these prophages, the 28-kb P4-like prophage YWA-4, encodes a PilV-like protein that may be used for adhesion to and invasion of the intestinal cells. Furthermore, a putative autotransporter and two type 1 fimbrial proteins of strain WA-314 show a sequence similarity Y. enterocolitica strains 8081 and WA-314 and thus the different efficiency of host colonization. Further important differences were found in two pYV plasmid-encoded virulence factors, YopM and YscP. The impact of these differences on virulence is discussed. Conclusions Our study emphasizes that the virulence of pathogens can be increased, by acquiring new genes and/or improving the function of essential virulence proteins, resulting in permanently hyper-virulent strains. This work also highlights the importance of addressing genetic and phenotypic variations among closely related bacterial strains, even those belonging to the same bioserotype.
- Published
- 2012
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5. Contribution of bacterial and host factors to pathogen "blooming" in a gnotobiotic mouse model for Salmonella enterica serovar Typhimurium-induced enterocolitis.
- Author
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Beutler M, Eberl C, Garzetti D, Herp S, Münch P, Ring D, Dolowschiak T, Brugiroux S, Schiller P, Hussain S, Basic M, Bleich A, and Stecher B
- Subjects
- Mice, Animals, Salmonella typhimurium, Serogroup, Bacteria, Inflammation, Disease Models, Animal, Germ-Free Life, Enterocolitis, Microbiota, Salmonella Infections, Animal microbiology
- Abstract
Inflammation has a pronounced impact on the intestinal ecosystem by driving an expansion of facultative anaerobic bacteria at the cost of obligate anaerobic microbiota. This pathogen "blooming" is also a hallmark of enteric Salmonella enterica serovar Typhimurium ( S . Tm) infection. Here, we analyzed the contribution of bacterial and host factors to S . Tm "blooming" in a gnotobiotic mouse model for S . Tm-induced enterocolitis. Mice colonized with the Oligo-Mouse-Microbiota (OMM
12 ), a minimal bacterial community, develop fulminant colitis by day 4 after oral infection with wild-type S . Tm but not with an avirulent mutant. Inflammation leads to a pronounced reduction in overall intestinal bacterial loads, distinct microbial community shifts, and pathogen blooming (relative abundance >50%). S . Tm mutants attenuated in inducing gut inflammation generally elicit less pronounced microbiota shifts and reduction in total bacterial loads. In contrast, S . Tm mutants in nitrate respiration, salmochelin production, and ethanolamine utilization induced strong inflammation and S . Tm "blooming." Therefore, individual Salmonella -specific inflammation-fitness factors seem to be of minor importance for competition against this minimal microbiota in the inflamed gut. Finally, we show that antibody-mediated neutrophil depletion normalized gut microbiota loads but not intestinal inflammation or microbiota shifts. This suggests that neutrophils equally reduce pathogen and commensal bacterial loads in the inflamed gut., Competing Interests: The authors declare no conflict of interest.- Published
- 2024
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6. CCL17 Promotes Colitis-Associated Tumorigenesis Dependent on the Microbiota.
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Metzger R, Winter L, Bouznad N, Garzetti D, von Armansperg B, Rokavec M, Lutz K, Schäfer Y, Krebs S, Winheim E, Friedrich V, Matzek D, Öllinger R, Rad R, Stecher B, Hermeking H, Brocker T, and Krug AB
- Subjects
- Mice, Animals, Carcinogenesis, Cell Transformation, Neoplastic, Azoxymethane toxicity, Chemokine CCL17, Colitis, Colonic Neoplasms pathology, Gastrointestinal Microbiome
- Abstract
Colorectal cancer is one of the most common cancers and a major cause of mortality. Proinflammatory and antitumor immune responses play critical roles in colitis-associated colon cancer. CCL17, a chemokine of the C-C family and ligand for CCR4, is expressed by intestinal dendritic cells in the steady state and is upregulated during colitis in mouse models and inflammatory bowel disease patients. In this study, we investigated the expression pattern and functional relevance of CCL17 for colitis-associated colon tumor development using CCL17-enhanced GFP-knockin mice. CCL17 was highly expressed by dendritic cells but also upregulated in macrophages and intermediary monocytes in colon tumors induced by exposure to azoxymethane and dextran sodium sulfate. Despite a similar degree of inflammation in the colon, CCL17-deficient mice developed fewer tumors than did CCL17-competent mice. This protective effect was abrogated by cohousing, indicating a dependency on the microbiota. Changes in microbiota diversity and composition were detected in separately housed CCL17-deficient mice, and these mice were more susceptible to azoxymethane-induced early apoptosis in the colon affecting tumor initiation. Immune cell infiltration in colitis-induced colon tumors was not affected by the lack of CCL17. Taken together, our results indicate that CCL17 promotes colitis-associated tumorigenesis by influencing the composition of the intestinal microbiome and reducing apoptosis during tumor initiation., (Copyright © 2022 by The American Association of Immunologists, Inc.)
- Published
- 2022
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7. Microbiome Analysis from Paired Mucosal and Fecal Samples of a Colorectal Cancer Biobank.
- Author
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Wirth U, Garzetti D, Jochum LM, Spriewald S, Kühn F, Ilmer M, Lee SML, Niess H, Bazhin AV, Andrassy J, Werner J, Stecher B, and Schiergens TS
- Abstract
The role of gut microbiota in colorectal cancer is subject to extensive research. Before usage of biorepositories for microbiome studies, it is crucial to evaluate technical feasibility of microbiome profiling from various biospecimens. The aim of this study was to assess the feasibility of DNA-extraction and microbiome profiling of samples from different sample sites, tissue sites and storage duration of a colorectal cancer biobank. Mucosa samples, mucosal scrapings and feces as well as different tissue sites (tumor, normal mucosa) were analyzed. 16S rRNA gene-based microbiome profiling with taxonomic assignment was performed on the Illumina MiSeq (Illumina, San Diego, USA) platform from stored snap frozen samples. For statistical analysis, α- and β-diversity measures, PCoA, permutational multivariate analysis of variance and graphical representation were performed. Microbiome analysis could be successfully performed in most of the samples (overall 93.3%) with sufficient numbers of high-quality reads. There were no differences between sample sites, while in some measures significant differences were found between tumor and normal mucosa (α-diversity, Shannon/Simpson Indices p = 0.028/0.027, respectively). Samples stored for up to eight years were used and storage conditions had no significant influence on the results. Tumor and tissue samples of a biobank stored long term can be successfully used for microbiome analysis. As large sample sizes are needed for association studies to evaluate microbial impact on tumorigenesis or progression of colorectal cancer, an already established biorepository may be a useful alternative to prospective clinical studies.
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- 2020
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8. Mucispirillum schaedleri Antagonizes Salmonella Virulence to Protect Mice against Colitis.
- Author
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Herp S, Brugiroux S, Garzetti D, Ring D, Jochum LM, Beutler M, Eberl C, Hussain S, Walter S, Gerlach RG, Ruscheweyh HJ, Huson D, Sellin ME, Slack E, Hanson B, Loy A, Baines JF, Rausch P, Basic M, Bleich A, Berry D, and Stecher B
- Subjects
- Animals, Colitis chemically induced, Disease Models, Animal, Germ-Free Life, Mice, Antibiosis, Bacteria, Anaerobic growth & development, Colitis prevention & control, Salmonella Infections prevention & control, Salmonella typhimurium growth & development
- Abstract
The microbiota and the gastrointestinal mucus layer play a pivotal role in protection against non-typhoidal Salmonella enterica serovar Typhimurium (S. Tm) colitis. Here, we analyzed the course of Salmonella colitis in mice lacking a functional mucus layer in the gut. Unexpectedly, in contrast to mucus-proficient littermates, genetically deficient mice were protected against Salmonella-induced gut inflammation in the streptomycin colitis model. This correlated with microbiota alterations and enrichment of the bacterial phylum Deferribacteres. Using gnotobiotic mice associated with defined bacterial consortia, we causally linked Mucispirillum schaedleri, currently the sole known representative of Deferribacteres present in the mammalian microbiota, to host protection against S. Tm colitis. Inhibition by M. schaedleri involves interference with S. Tm invasion gene expression, partly by competing for anaerobic electron acceptors. In conclusion, this study establishes M. schaedleri, a core member of the murine gut microbiota, as a key antagonist of S. Tm virulence in the gut., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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9. Usability of rectal swabs for microbiome sampling in a cohort study of hematological and oncological patients.
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Biehl LM, Garzetti D, Farowski F, Ring D, Koeppel MB, Rohde H, Schafhausen P, Stecher B, and Vehreschild MJGT
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- Cohort Studies, Female, Humans, Male, Enterobacteriaceae classification, Enterobacteriaceae growth & development, Enterococcus classification, Enterococcus genetics, Feces microbiology, Gastrointestinal Microbiome, Hematologic Neoplasms microbiology, Specimen Handling
- Abstract
Objectives: Large-scale clinical studies investigating associations between intestinal microbiota signatures and human diseases usually rely on stool samples. However, the timing of repeated stool sample collection cannot be predefined in longitudinal settings. Rectal swabs, being straightforward to obtain, have the potential to overcome this drawback. Therefore, we assessed the usability of rectal swabs for microbiome sampling in a cohort of hematological and oncological patients., Study Design: We used a pipeline for intestinal microbiota analysis from deep rectal swabs which was established and validated with test samples and negative controls. Consecutively, a cohort of patients from hematology and oncology wards was established and weekly deep rectal swabs taken during their admissions and re-admissions., Results: Validation of our newly developed pipeline for intestinal microbiota analysis from rectal swabs revealed consistent and reproducible results. Over a period of nine months, 418 rectal swabs were collected longitudinally from 41 patients. Adherence to the intended sampling protocol was 97%. After DNA extraction, sequencing, read pre-processing and filtering of chimeric sequences, 405 of 418 samples (96.9%) were eligible for further analyses. Follow-up samples and those taken under current antibiotic exposure showed a significant decrease in alpha diversity as compared to baseline samples. Microbial domination occurred most frequently by Enterococcaceae (99 samples, 24.4%) on family level and Enterococcus (90 samples, 22.2%) on genus level. Furthermore, we noticed a high abundance of potential skin commensals in 99 samples (24.4%)., Summary: Deep rectal swabs were shown to be reliable for microbiome sampling and analysis, with practical advantages related to high sampling adherence, easy timing, transport and storage. The relatively high abundance of putative skin commensals in this patient cohort may be of potential interest and should be further investigated. Generally, previous findings on alpha diversity dynamics obtained from stool samples were confirmed., Competing Interests: LMB has received lecture honoraria from Astellas Pharma and MSD and travel grants from 3M and Gilead. MJGTV has served at the speakers’ bureau of Pfizer, Merck, Gilead Sciences and Astellas Pharma, received research funding from 3M, DaVolterra, Astellas Pharma, Gilead Sciences and Merck and is a consultant to Berlin Chemie, Astellas Pharma and Merck. All other authors: none to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2019
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10. Complete Genome Sequencing of the Mouse Intestinal Isolate Escherichia coli Mt1B1.
- Author
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Garzetti D, Eberl C, and Stecher B
- Abstract
Escherichia coli Mt1B1, a mouse isolate, is a facultative anaerobic bacterium which was shown to counteract Salmonella enterica serovar Typhimurium infection in a mouse model. In the present study, we describe the complete genome sequence of E. coli Mt1B1, composed of a 5.1-Mb chromosome and a 62.6-kb plasmid., (Copyright © 2018 Garzetti et al.)
- Published
- 2018
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11. Microbiota differences between commercial breeders impacts the post-stroke immune response.
- Author
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Sadler R, Singh V, Benakis C, Garzetti D, Brea D, Stecher B, Anrather J, and Liesz A
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- Animals, CD28 Antigens administration & dosage, CD28 Antigens immunology, Encephalitis immunology, Encephalitis metabolism, Female, Immunotherapy, Lymphocyte Activation, Male, Mice, Inbred C57BL, Reproducibility of Results, Stroke therapy, Gastrointestinal Microbiome, Stroke immunology, T-Lymphocytes physiology
- Abstract
Experimental reproducibility between laboratories is a major translational obstacle worldwide, particularly in studies investigating immunomodulatory therapies in relation to brain disease. In recent years increasing attention has been drawn towards the gut microbiota as a key factor in immune cell polarization. Moreover, manipulation of the gut microbiota has been found effective in a diverse range of brain disorders. Within this study we aimed to test the impact of microbiota differences between mice from different sources on the post-stroke neuroinflammatory response. With this rationale, we have investigated the correlation between microbiota differences and the immune response in mice from three commercial breeders with the same genetic background (C57BL/6). While overall bacterial load was comparable, we detected substantial differences in species diversity and microbiota composition on lower taxonomic levels. Specifically, we investigated segmented filamentous bacteria (SFB)-which have been shown to promote T cell polarization-and found that they were absent in mice from one breeder but abundant in others. Our experiments revealed a breeder specific correlation between SFB presence and the ratio of Treg to Th17 cells. Moreover, recolonization of SFB-negative mice with SFB resulted in a T cell shift which mimicked the ratios found in SFB-positive mice. We then investigated the response to a known experimental immunotherapeutic approach, CD28 superagonist (CD28SA), which has been previously shown to expand the Treg population. CD28SA treatment had differing effects between mice from different breeders and was found to be ineffective at inducing Treg expansion in SFB-free mice. These changes directly corresponded to stroke outcome as mice lacking SFB had significantly larger brain infarcts. This study demonstrates the major impact of microbiota differences on T cell polarization in mice during ischemic stroke conditions, and following immunomodulatory therapies., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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12. High-Quality Whole-Genome Sequences of the Oligo-Mouse-Microbiota Bacterial Community.
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Garzetti D, Brugiroux S, Bunk B, Pukall R, McCoy KD, Macpherson AJ, and Stecher B
- Abstract
The Oligo-Mouse-Microbiota (Oligo-MM
12 ) is a community of 12 mouse intestinal bacteria to be used for microbiome research in gnotobiotic mice. We present here the high-quality whole genome sequences of the Oligo-MM12 strains, which were obtained by combining the accuracy of the Illumina platforms with the long reads of the PacBio technology., (Copyright © 2017 Garzetti et al.)- Published
- 2017
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13. Erratum: CD40-signalling abrogates induction of RORγt + Treg cells by intestinal CD103 + DCs and causes fatal colitis.
- Author
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Barthels C, Ogrinc A, Steyer V, Meier S, Simon F, Wimmer M, Blutke A, Straub T, Zimber-Strobl U, Lutgens E, Marconi P, Ohnmacht C, Garzetti D, Stecher B, and Brocker T
- Published
- 2017
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14. CD40-signalling abrogates induction of RORγt + Treg cells by intestinal CD103 + DCs and causes fatal colitis.
- Author
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Barthels C, Ogrinc A, Steyer V, Meier S, Simon F, Wimmer M, Blutke A, Straub T, Zimber-Strobl U, Lutgens E, Marconi P, Ohnmacht C, Garzetti D, Stecher B, and Brocker T
- Subjects
- Animals, Antigens, CD immunology, Antigens, CD metabolism, CD40 Antigens genetics, CD40 Antigens metabolism, Colitis genetics, Colitis metabolism, Dendritic Cells metabolism, Gastrointestinal Microbiome immunology, Integrin alpha Chains immunology, Integrin alpha Chains metabolism, Intestinal Mucosa metabolism, Intestines immunology, Intestines microbiology, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Nuclear Receptor Subfamily 1, Group F, Member 3 immunology, Nuclear Receptor Subfamily 1, Group F, Member 3 metabolism, T-Lymphocytes, Regulatory metabolism, Th1 Cells immunology, Th1 Cells metabolism, Th17 Cells immunology, Th17 Cells metabolism, CD40 Antigens immunology, Colitis immunology, Dendritic Cells immunology, Signal Transduction immunology, T-Lymphocytes, Regulatory immunology
- Abstract
Immune homeostasis in intestinal tissues depends on the generation of regulatory T (Treg) cells. CD103
+ dendritic cells (DCs) acquire microbiota-derived material from the gut lumen for transport to draining lymph nodes and generation of receptor-related orphan γt+ (RORγt+ ) Helios- -induced Treg (iTreg) cells. Here we show CD40-signalling as a microbe-independent signal that can induce migration of CD103+ DCs from the lamina propria (LP) to the mesenteric lymph nodes. Transgenic mice with constitutive CD11c-specific CD40-signalling have reduced numbers of CD103+ DCs in LP and a low frequency of RORγt+ Helios- iTreg cells, exacerbated inflammatory Th1/Th17 responses, high titres of microbiota-specific immunoglobulins, dysbiosis and fatal colitis, but no pathology is detected in other tissues. Our data demonstrate a CD40-dependent mechanism capable of abrogating iTreg cell induction by DCs, and suggest that the CD40L/CD40-signalling axis might be able to intervene in the generation of new iTreg cells in order to counter-regulate immune suppression to enhance immunity.- Published
- 2017
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15. Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium.
- Author
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Brugiroux S, Beutler M, Pfann C, Garzetti D, Ruscheweyh HJ, Ring D, Diehl M, Herp S, Lötscher Y, Hussain S, Bunk B, Pukall R, Huson DH, Münch PC, McHardy AC, McCoy KD, Macpherson AJ, Loy A, Clavel T, Berry D, and Stecher B
- Subjects
- Animals, Mice, Antibiosis, Gastrointestinal Microbiome, Gastrointestinal Tract microbiology, Salmonella Infections, Animal prevention & control, Salmonella typhimurium physiology
- Abstract
Protection against enteric infections, also termed colonization resistance, results from mutualistic interactions of the host and its indigenous microbes. The gut microbiota of humans and mice is highly diverse and it is therefore challenging to assign specific properties to its individual members. Here, we have used a collection of murine bacterial strains and a modular design approach to create a minimal bacterial community that, once established in germ-free mice, provided colonization resistance against the human enteric pathogen Salmonella enterica serovar Typhimurium (S. Tm). Initially, a community of 12 strains, termed Oligo-Mouse-Microbiota (Oligo-MM
12 ), representing members of the major bacterial phyla in the murine gut, was selected. This community was stable over consecutive mouse generations and provided colonization resistance against S. Tm infection, albeit not to the degree of a conventional complex microbiota. Comparative (meta)genome analyses identified functions represented in a conventional microbiome but absent from the Oligo-MM12 . By genome-informed design, we created an improved version of the Oligo-MM community harbouring three facultative anaerobic bacteria from the mouse intestinal bacterial collection (miBC) that provided conventional-like colonization resistance. In conclusion, we have established a highly versatile experimental system that showed efficacy in an enteric infection model. Thus, in combination with exhaustive bacterial strain collections and systems-based approaches, genome-guided design can be used to generate insights into microbe-microbe and microbe-host interactions for the investigation of ecological and disease-relevant mechanisms in the intestine.- Published
- 2016
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16. Corrigendum: The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota.
- Author
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Lagkouvardos I, Pukall R, Abt B, Foesel BU, Meier-Kolthoff JP, Kumar N, Bresciani A, Martínez I, Just S, Ziegler C, Brugiroux S, Garzetti D, Wenning M, Bui TP, Wang J, Hugenholtz F, Plugge CM, Peterson DA, Hornef MW, Baines JF, Smidt H, Walter J, Kristiansen K, Nielsen HB, Haller D, Overmann J, Stecher B, and Clavel T
- Published
- 2016
- Full Text
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17. The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota.
- Author
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Lagkouvardos I, Pukall R, Abt B, Foesel BU, Meier-Kolthoff JP, Kumar N, Bresciani A, Martínez I, Just S, Ziegler C, Brugiroux S, Garzetti D, Wenning M, Bui TP, Wang J, Hugenholtz F, Plugge CM, Peterson DA, Hornef MW, Baines JF, Smidt H, Walter J, Kristiansen K, Nielsen HB, Haller D, Overmann J, Stecher B, and Clavel T
- Subjects
- Animals, Bacteria classification, Bacteria genetics, Gastrointestinal Microbiome genetics, Genome, Bacterial, Mice, Bacteria isolation & purification, Biodiversity, Biological Specimen Banks, Gastrointestinal Microbiome physiology, Host Specificity, Intestines microbiology
- Abstract
Intestinal bacteria influence mammalian physiology, but many types of bacteria are still uncharacterized. Moreover, reference strains of mouse gut bacteria are not easily available, although mouse models are extensively used in medical research. These are major limitations for the investigation of intestinal microbiomes and their interactions with diet and host. It is thus important to study in detail the diversity and functions of gut microbiota members, including those colonizing the mouse intestine. To address these issues, we aimed at establishing the Mouse Intestinal Bacterial Collection (miBC), a public repository of bacterial strains and associated genomes from the mouse gut, and studied host-specificity of colonization and sequence-based relevance of the resource. The collection includes several strains representing novel species, genera and even one family. Genomic analyses showed that certain species are specific to the mouse intestine and that a minimal consortium of 18 strains covered 50-75% of the known functional potential of metagenomes. The present work will sustain future research on microbiota-host interactions in health and disease, as it will facilitate targeted colonization and molecular studies. The resource is available at www.dsmz.de/miBC.
- Published
- 2016
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18. A molecular scheme for Yersinia enterocolitica patho-serotyping derived from genome-wide analysis.
- Author
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Garzetti D, Susen R, Fruth A, Tietze E, Heesemann J, and Rakin A
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- Genotype, Humans, Multigene Family, O Antigens genetics, Polymerase Chain Reaction methods, Serotyping, Yersinia Infections diagnosis, Yersinia Infections microbiology, Genome, Bacterial, Genotyping Techniques, Molecular Typing methods, Yersinia enterocolitica classification, Yersinia enterocolitica genetics
- Abstract
Yersinia enterocolitica is a food-borne, gastro-intestinal pathogen with world-wide distribution. Only 11 serotypes have been isolated from patients, with O:3, O:9, O:8 and O:5,27 being the serotypes most commonly associated with human yersiniosis. Serotype is an important characteristic of Y. enterocolitica strains, allowing differentiation for epidemiology, diagnosis and phylogeny studies. Conventional serotyping, performed by slide agglutination, is a tedious and laborious procedure whose interpretation tends to be subjective, leading to poor reproducibility. Here we present a PCR-based typing scheme for molecular identification and patho-serotyping of Y. enterocolitica. Genome-wide comparison of Y. enterocolitica sequences allowed analysis of the O-antigen gene clusters of different serotypes, uncovering their formerly unknown genomic locations, and selection of targets for serotype-specific amplification. Two multiplex PCRs and one additional PCR were designed and tested on various reference strains and isolates from different origins. Our genotypic assay proved to be highly specific for identification of Y. enterocolitica species, discrimination between virulent and non-virulent strains, distinguishing the main human-related serotypes, and typing of conventionally untypeable strains. This genotyping scheme could be applied in microbiology laboratories as an alternative or complementary method to the traditional phenotypic assays, providing data for epidemiological studies., (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Published
- 2014
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19. Unique activity spectrum of colicin FY: all 110 characterized Yersinia enterocolitica isolates were colicin FY susceptible.
- Author
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Bosák J, Micenková L, Vrba M, Ševčíková A, Dědičová D, Garzetti D, and Šmajs D
- Subjects
- Microbial Sensitivity Tests, Virulence Factors genetics, Yersinia enterocolitica isolation & purification, Colicins pharmacology, Drug Resistance, Bacterial drug effects, Genome, Bacterial, Virulence Factors metabolism, Yersinia enterocolitica genetics, Yersinia enterocolitica metabolism
- Abstract
Colicin FY is a plasmid encoded toxin that recognizes a yersinia-specific outer membrane protein (YiuR) as a receptor molecule. We have previously shown that the activity spectrum of colicin FY comprises strains of the genus Yersinia. In this study, we analyzed the activity of colicin FY against 110 Yersinia enterocolitica isolates differing in geographical origin and source. All isolates were characterized through analysis of 16S rRNA genes, serotyping, biotyping, restriction profiling of genomic DNA, detection of virulence markers and susceptibility to antibiotics. This confirmed the broad variability of the collection, in which all 110 Y. enterocolitica isolates, representing 77 various strains, were inhibited by colicin FY. Although isolates showed variable levels of susceptibility to colicin FY, it was not associated with any strain characteristic. The universal susceptibility of Y. enterocolitica strains to colicin FY together with the absence of activity towards strains outside the Yersinia genus suggests potential therapeutic applications for colicin FY.
- Published
- 2013
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20. Genome Sequences of Four Yersinia enterocolitica Bioserotype 4/O:3 Isolates from Mammals.
- Author
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Garzetti D, Heesemann J, and Rakin A
- Abstract
We report here the complete genome sequences of four European Yersinia enterocolitica mammalian isolates of bioserotype 4/O:3. The genomes have an average size of 4.50 Mb, a G+C content of 47%, and between 4,231 and 4,330 coding sequences (CDSs). No relevant differences were detected by genome comparison between mammalian and human isolates.
- Published
- 2013
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21. Gains and Losses in Yersinia enterocolitica subsp. palearctica Genomes.
- Author
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Rakin A, Batzilla J, Garzetti D, and Heesemann J
- Subjects
- Genome, Bacterial, Phylogeny, Yersinia enterocolitica classification, Yersinia enterocolitica genetics
- Published
- 2012
- Full Text
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