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We have developed a new and simple method for quantitatively analyzing global gene expression profiles from cells or tissues. The process, called TALEST, or tandem arrayed ligation of expressed sequence tags, employs an oligonucleotide adapter containing a type IIs restriction enzyme site to facilitate the generation of short (16 bp) ESTs of fixed position in the mRNA. These ESTs are flanked by GC-clamped punctuation sequences which render them resistant to thermal denaturation, allowing their concatenation into long arrays and subsequent recognition and analysis by high-throughput DNA sequencing. A major advantage of the TALEST technique is the avoidance of PCR in all stages of the process and hence the attendant sequence-specific amplification biases that are inherent in other gene expression profiling methods such as SAGE, Differential Display, AFLP, etc. which rely on PCR.

The number of facilities across the country "implementing" some "flavor" of the SIGGRAPH/GSPC Core System "strawman standard" ["Status Report of the Graphic Standards Planning Committee of ACM/SIGGRAPH", Computer Graphics, Vol. II, Number 3, Fall 1977] has reached approximately fifteen. To keep the graphics community informed about the reception and usage of the Core System, we have collected descriptions of the status of each facility, as reproduced here. An Implementor's Forum will occur in November '78, which we hope will provide both significant interfacility communication and some concensus among implementors on such topics as FORTRAN '77 compatible calling sequences for Core System routines, the structure of intermediate (neutral, pseudo-, etc.) display files, and techniques and algorithms for viewing, clipping, picking, and echoing.