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Articular cartilage degeneration poses a significant public health challenge; techniques such as 3D bioprinting are being explored for its regeneration in vitro. Gelatin-based hydrogels represent one of the most promising biopolymers used in cartilage tissue engineering, especially for its collagen composition and tunable mechanical properties. However, there are no standard protocols that define process parameters such as the crosslinking method to apply. To this aim, a reproducible study was conducted for exploring the influence of different crosslinking methods on 3D bioprinted gelatin structures. This study assessed mechanical properties and cell viability in relation to various crosslinking techniques, revealing promising results particularly for dual (photo + ionic) crosslinking methods, which achieved high cell viability and tunable stiffness. These findings offer new insights into the effects of crosslinking methods on 3D bioprinted gelatin for cartilage applications. For example, ionic and photo-crosslinking methods provide softer materials, with photo-crosslinking supporting cell stretching and diffusion, while ionic crosslinking preserves a spherical stem cell morphology. On the other hand, dual crosslinking provides a stiffer, optimized solution for creating stable cartilage-like constructs. The results of this study offer a new perspective on the standardization of gelatin for cartilage bioprinting, bridging the gap between research and clinical applications. [ABSTRACT FROM AUTHOR]

Articular cartilage morphology and composition are essential factors in joint biomechanics, and their alteration is a crucial aspect of osteoarthritis (OA), a prevalent disease that causes pain and functional loss. This research focuses on developing patient-specific synthetic cartilage using innovative Digital Anatomy polymers. The objectives include investigating the morphology, characterizing the mechanical properties, and replicating the architecture of natural cartilage. This approach offers potential alternatives to traditional manufacturing methods and reduces the need for expensive in vivo experiments. Finite Element Analysis (FEA) validates a novel patient-specific measurement setup. It provides insights into the role of morphology in the distribution of stress and strain within cartilage. CAD design is also utilized to create standardized fiber-reinforced samples that mimic the layered micro-architecture of natural cartilage, allowing for the study of their contribution to the overall mechanical properties. The results demonstrate that 3D-printed polymers can effectively replicate the elastic properties of cartilage. The proposed patient-specific simulator produces reliable results, which have been validated through FEM analysis. While the recreated microstructure closely resembles biological cartilage samples, the elastic properties are slightly underestimated. In conclusion, designing an in silico knee joint is a feasible approach that offers numerous advantages for further development. The Young's modulus values of our synthetic cartilage modules range from 2.43 MPa to 7.24 MPa, within the range reported in the literature. Moreover, Young´s modulus at the micro level shows the differences between surface 1.74 MPa and internal substrate 1.83 MPa depending on the fiber orientation. Finally, our model proves to be mechanically and morphologically accurate at both the macro and micro levels. [ABSTRACT FROM AUTHOR]

At the end of the 2023 Padua Days of Muscle and Mobility Medicine the next year's meeting was scheduled from 27 February to 2 March 2024 (2024Pdm3). During the summer and autumn the program was confirmed with Scientific Sessions that will take place over five days, starting in the afternoon of February 27, 2024 at the Conference Room of the Hotel Petrarca, Thermae of Euganean Hills (Padua), Italy. As usual, the next day will be spent in Padua, in this occasion at the San Luca Hall of the Santa Giustina monastery in Prato della Valle, Padua, Italy. Collected during Autumn 2023, many more titles and abstracts than expected were submitted, forcing the organization of parallel sessions both on March 1 and March 2 2024 confirming attractiveness of the 2024 Pdm3. The five days will include oral presentations of scientists and clinicians from Argentina, Austria, Belgium, Brazil, Canada, Denmark, Egypt, France, Germany, Iceland, Ireland, Italy, Romania, Russia, Slovenia, Switzerland, UK and USA. Together with the preliminary Program at December 1, 2023, the early submitted Abstracts is e-published in this Issue 33 (4) 2023 of the European Journal of Translational Myology (EJTM). You are invited to join, submitting your Last Minute Abstracts to ugo.carraro@unipd.it by February 1, 2024. Furthermore, with the more generous deadline of May 20, 2024, submit please “Communications” to the European Journal of Translational Myology (Clarivate’s ESCI Impact factor 2.2; SCOPUS Cite Score: 3.2). See you soon at the Hotel Petrarca in Montegrotto Terme, Padua, on February 27, 2024, but the complete program can be followed from home via zoom connection. [ABSTRACT FROM AUTHOR]