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Your search keyword '"Garcia, Valeria S."' showing total 19 results
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19 results on '"Garcia, Valeria S."'

15. Carboxyl fuchsine-based colored particles for application to immunodetection.

Author

Femia, A. Lis, Gonzalez, Verónica D. G., Garcia, Valeria S., and Gugliotta, Luis M.

Subjects
BINDING site assay, SURFACE charges, MEDICAL research
Abstract

Functionalized latex particles (FLP) are normally applied for diagnosis and therapeutics purposes and, in particular, the interest in colored FLP is rising in areas such as biomedical and research. The synthesis, characterization and application of FLP with a new colored monomer is here considered, in a simple and fast manner, and at low cost. Synthesis variables were controlled to obtain particles of different size, surface charge and color incorporation. Then, particles were proved in protein binding assays; and the development of an immunochromatographic test was successfully performed. [ABSTRACT FROM AUTHOR]

Published
2022
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18. Latex particles sensitized with proteins of Leptospira interrogans for application in immunoagglutination assays.

Author

Prochetto, Santiago D., Gonzalez, Verónica D. G., Garcia, Valeria S., Vanasco, Norma B., Lottersberger, Javier, and Gugliotta, Luis M.

Subjects
PHYSISORPTION, COUPLING reactions (Chemistry), LEPTOSPIRA interrogans, LATEX, CEMENTATION (Petrology)
Abstract

Physical adsorption and chemical coupling of recombinant proteins ofLeptospira interrogansonto polystyrene and core-shell carboxylated particles were, respectively, investigated with the aim of producing latex–protein complexes to be used in immunoagglutination assays able to detect the leptospirosis disease in either humans or animals. To this effect, a protein lysate of crude extracts was evaluated, and sensitizations were carried out at different pHs, with the antigenic proteins approach to the particle surface favored at pH close to their isoelectric point. In the covalent coupling experiments, high fractions of proteins were chemically bound to carboxyl groups on the particle surface and higher densities of linked proteins were obtained for particles exhibiting greater carboxyl group densities. The produced latex–protein complexes were tested in immunoagglutination assays, by turbidimetry and a visual method, from a panel that included positive and negative bovine, canine, and human sera. The area under receiver operating characteristic curves was used as an index of accuracy. The complexes obtained by covalent coupling of proteins on the latex of higher density of carboxyl groups allowed an acceptable discrimination between the studied positive and negative sera. [ABSTRACT FROM PUBLISHER]

Published
2017
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