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33 results on '"Garcia, Denisse"'

5. Adipocyte‐derived inflammatory molecules induce senescent B cells through metabolic pathways.

Author

Frasca, Daniela, Romero, Maria, Garcia, Denisse, Thaller, Seth, and Bueno, Valquiria

Subjects
B cells, AEROBIC capacity, WEIGHT loss, GALACTOSIDASES, POLYMERASE chain reaction, CELLULAR aging
Abstract

Objective: The aim of this study was to demonstrate that an adipocyte tissue‐derived conditioned medium (ACM) contains inflammatory molecules that induce senescence in B cells. Methods: We incubated blood‐derived B cells from lean donors with ACM obtained from the adipose tissue of adult female donors with obesity undergoing weight reduction surgery or with medium as control. After 24 h, cells were harvested, and the expression of transcripts for proinflammatory cytokines (TNF/IL‐6), chemokines (IL‐8), and for markers of the senescence‐associated secretory phenotype (SASP) was measured by quantitative polymerase chain reaction. B cells were also stained with the marker of immunosenescence β‐galactosidase, and their metabolic status was evaluated in Seahorse using a Mito Stress Test. Results: We show that the incubation of B cells from lean donors with ACM induces the expression of transcripts for inflammatory and SASP transcripts, increases the amount of β‐galactosidase staining, and induces a metabolic phenotype characterized by higher basal and maximal oxygen consumption, spare respiratory capacity (difference between maximal and basal respiration), nonmitochondrial oxygen consumption, ATP production, and proton leak. Conclusions: These results demonstrate that B cells from lean individuals, after incubation with ACM, become inflammatory and senescent, and this occurs through metabolic pathways needed to support their secretory phenotype. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Induction of antigen specific intrahepatic CD8+ T cell responses by a secreted heat shock protein based gp96-Ig-PfCA malaria vaccine

Author

Padula, Laura, primary, Fisher, Eva, additional, Wijayalath, Wathsala, additional, Patterson, Noelle B., additional, Huang, Jun, additional, Ganeshan, Harini, additional, Robinson, Tanisha, additional, Bates, François A., additional, Hanson, Margaret A., additional, Martin, Monica L., additional, Rivas, Katelyn, additional, Garcia, Denisse, additional, Edgel, Kimberly A., additional, Sedegah, Martha, additional, Villasante, Eileen, additional, and Strbo, Natasa, additional

Published
2023
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14. A Battle Royale: Embedding Versus the Exclusive Right to Display.

Author

Galli, Nicole D. and Garcia, Denisse

Subjects
Fair use (Copyright) -- Laws, regulations and rules, Copyright infringement -- Analysis -- Laws, regulations and rules, Government regulation, Company business management, OLE, Copyright Act of 1976 (17 U.S.C. 101)
Abstract

The digital revolution has changed how we communicate--sharing and creating imagery online is no longer optional. With our collective attention span shrinking, the old adage that "a picture is worth [...]

Published
2022

16. Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Author

Pastar, Irena, O’Neill, Katelyn, Padula, Laura, Head, Cheyanne R., Burgess, Jamie L., Chen, Vivien, Garcia, Denisse, Stojadinovic, Olivera, Hower, Suzanne, Plano, Gregory V., Thaller, Seth R., Tomic-Canic, Marjana, and Strbo, Natasa

Subjects
perforin-2/mpeg-1, Immunology, Staphylococcus epidermidis, Correction, Immunology and Allergy, gamma delta T cells, cytotoxicity, human skin, Immunologic diseases. Allergy, RC581-607, innate immunity
Published
2021
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17. Corrigendum: Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Author

Pastar, Irena, primary, O’Neill, Katelyn, additional, Padula, Laura, additional, Head, Cheyanne R., additional, Burgess, Jamie L., additional, Chen, Vivien, additional, Garcia, Denisse, additional, Stojadinovic, Olivera, additional, Hower, Suzanne, additional, Plano, Gregory V., additional, Thaller, Seth R., additional, Tomic-Canic, Marjana, additional, and Strbo, Natasa, additional

Published
2021
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19. B Cell Immunosenescence

Author

Frasca, Daniela, primary, Diaz, Alain, additional, Romero, Maria, additional, Garcia, Denisse, additional, and Blomberg, Bonnie B., additional

Published
2020
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20. Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Author

Pastar, Irena, primary, O’Neill, Katelyn, additional, Padula, Laura, additional, Head, Cheyanne R., additional, Burgess, Jamie L., additional, Chen, Vivien, additional, Garcia, Denisse, additional, Stojadinovic, Olivera, additional, Hower, Suzanne, additional, Plano, Gregory V., additional, Thaller, Seth R., additional, Tomic-Canic, Marjana, additional, and Strbo, Natasa, additional

Published
2020
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24. Secreted heat shock protein gp96-Ig vaccine induces malaria specific intrahepatic CD8 T cell responses

Author

Strbo, Natasa, primary, Padula, Laura, additional, Fisher, Eva, additional, Wijayalath, Wathsala, additional, Patterson, Noelle B., additional, Huang, Jun, additional, Ganeshan, Harini, additional, O’Neill, Katelyn, additional, Garcia, Denisse, additional, Etobayeva, Irina V., additional, Edgel, Kimberly A., additional, Podack, Eckhard R, additional, Sedegah, Martha, additional, and Villasante, Eileen F., additional

Published
2020
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27. Trade Secrets: The Fashion Industry's Trendiest Secret.

Author

Garcia, Denisse

Subjects
*TRADE secrets, *CLOTHING industry, *PROTECTION of trade secrets, *PATENT infringement, *CONSUMER behavior, *ONLINE marketplaces, *COVENANTS not to compete, *FORGERY, *BUSINESS planning
Published
2022

28. Single cell analyses reveal specific distribution of anti-bacterial molecule Perforin-2 in human skin and its modulation by wounding and Staphylococcus aureus infection

Author

Strbo, Natasa, primary, Pastar, Irena, additional, Romero, Laura, additional, Chen, Vivien, additional, Vujanac, Milos, additional, Sawaya, Andrew P., additional, Jozic, Ivan, additional, Ferreira, Andrea D. F., additional, Wong, Lulu L., additional, Head, Cheyanne, additional, Stojadinovic, Olivera, additional, Garcia, Denisse, additional, O'Neill, Katelyn, additional, Drakulich, Stefan, additional, Taller, Seth, additional, Kirsner, Robert S., additional, and Tomic-Canic, Marjana, additional

Published
2019
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31. Identification and Characterization of Adipose Tissue-Derived Human Antibodies With "Anti-self" Specificity.

Author

Frasca, Daniela, Diaz, Alain, Romero, Maria, Garcia, Denisse, Jayram, Diya, Thaller, Seth, del Carmen Piqueras, Maria, Bhattacharya, Sanjoy, and Blomberg, Bonnie B.

Subjects
IMMUNOSPECIFICITY, IMMUNOGLOBULINS, CELL death, ADIPOSE tissues, B cells
Abstract

We have previously shown that the human obese adipose tissue (AT) contributes to increased secretion of adipocyte-specific IgG antibodies in individuals with obesity. This occurs without any exogenous stimulation, because the ongoing process of cell death in the obese AT leads to the release of "self" antigens able to induce chronic stimulation of B cells. We have identified several mechanisms responsible for the release of "self" antigens, such as hypoxia, cell cytotoxicity, and DNA damage. In this paper, we confirm and extend our initial observation on a different cohort of individuals, and we show that also the plasma of these individuals is enriched in IgG antibodies with specificities for adipocyte-derived antigens. Adipocyte-specific IgG secreted in the obese AT are significantly correlated with those present in plasma. Using immunoprecipitation and mass spectrometry, we have identified these antigenic specificities. The antigens are almost exclusively intracellular or cell-associated, usually not recognized as "self" antigens, but they are released by cells dying in the AT. We also show for the first time that the adipocytes in the obese AT contribute to the secretion of IgG autoimmune antibodies and this seems to be due to their expression of the antigen-presenting molecules CD1d and, to a much lesser extent, MHC class II, as our mechanistic experiments performed in mice have shown. These results may lead to the development of novel therapeutic strategies to control autoimmunity. [ABSTRACT FROM AUTHOR]

Published
2020
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32. Peña Nieto and the Unions.

Author

Garcia, Denisse

Subjects
ECONOMIC development, EDUCATIONAL change, TEACHERS' unions, ECONOMIC indicators
Abstract

The article discusses the signed educational reform law of Mexico President Enrique Peña Nieto and the National Union of Education Workers (SNTE). It mentions that the president makes the move to combat the excessive authority and corruption found in the teachers' union especially in the practice of hiring and evaluating teachers for better Mexico's struggling educational system. It adds that the president wishes for economic growth and educational system improvement in Mexico.

Published
2013

33. Immunometabolic effects of lactate on B cell function in healthy individuals of different ages.

Author

Romero M, Miller K, Gelsomini A, Garcia D, Li K, Suresh D, and Frasca D

Abstract

Aging is characterized by chronic systemic inflammation and metabolic changes. When we compared B cells from young and elderly donors, we found that aging induces higher oxygen consumption rates, and especially higher extracellular acidification rates, measures of oxidative phosphorylation and of anaerobic glycolysis, respectively. Importantly, this higher metabolic status, which reflects the age-associated expansion of pro-inflammatory B cell subsets, was found associated with higher secretion of lactate and autoimmune antibodies after in vitro stimulation. B cells from elderly individuals, induce in vitro generation of pro-inflammatory CD4+ T cells from young individuals through metabolic pathways mediated by lactate secretion. Lactate also induces immunosenescent B cells that are glycolytic and express transcripts for multiple pro-inflammatory molecules. These results altogether may have relevant clinical implications and suggest novel targets for therapeutic interventions in patients with inflammatory conditions and diseases.

Published
2023
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