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1. Dietary squalene supplementation decreases triglyceride species and modifies phospholipid lipidomic profile in the liver of a porcine model of non-alcoholic steatohepatitis

Author

Herrera-Marcos, Luis V., Martínez-Beamonte, Roberto, Arnal, Carmen, Barranquero, Cristina, Puente-Lanzarote, Juan J., Herrero-Continente, Tania, Lou-Bonafonte, José M., Gonzalo-Romeo, Gonzalo, Mocciaro, Gabriele, Jenkins, Benjamin, Surra, Joaquín C., Rodríguez-Yoldi, María J., Burillo, Juan Carlos, Lasheras, Roberto, García-Gil, Agustín, Güemes, Antonio, Koulman, Albert, and Osada, Jesús

Published
2023
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2. Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Author

Herrera-Marcos, Luis V., Martínez-Beamonte, Roberto, Macías-Herranz, Manuel, Arnal, Carmen, Barranquero, Cristina, Puente-Lanzarote, Juan J., Gascón, Sonia, Herrero-Continente, Tania, Gonzalo-Romeo, Gonzalo, Alastrué-Vera, Víctor, Gutiérrez-Blázquez, Dolores, Lou-Bonafonte, José M., Surra, Joaquín C., Rodríguez-Yoldi, María J., García-Gil, Agustín, Güemes, Antonio, and Osada, Jesús

Published
2022
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3. Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine nonalcoholic steatohepatitis

Author

Herrera-Marcos, Luis V., primary, Martínez-Beamonte, Roberto, additional, Arnal, Carmen, additional, Barranquero, Cristina, additional, Puente-Lanzarote, Juan J., additional, Lou-Bonafonte, José M., additional, Gonzalo-Romeo, Gonzalo, additional, Mocciaro, Gabriele, additional, Jenkins, Benjamin, additional, Surra, Joaquín C., additional, Rodríguez-Yoldi, María J., additional, Alastrué-Vera, Víctor, additional, Letosa, Jesús, additional, García-Gil, Agustín, additional, Güemes, Antonio, additional, Koulman, Albert, additional, and Osada, Jesús, additional

Published
2024
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4. A Survey of Heads of Surgery and Residents on Accessibility and Employment in Liver Transplant Teams in Spain

Author

Blanco Fernández, Gerardo, Gómez Bravo, Miguel Ángel, Briceño Delgado, Javier, Valdivieso López, Andrés, López-Guerra, Diego, Rodríguez-Laiz, Gonzalo, Villar-del-Moral, Jesús, Pardo Sánchez, Fernando, Santoyo, Julio, López Andújar, Rafael, Nuño, Javier, Sánchez Turrión, Víctor, González Pinto, Ignacio, Jiménez, Carlos, López Baena, José Ángel, Gómez Gutierrez, Manuel, Fabregat Prous, Joan, Pacheco Sánchez, David, Barrera, Manuel, García-Gil, Agustín, Ramírez, Pablo, Varo, Evaristo, Bilbao, Itxarone, Fondevila, Constantino, and Rodríguez-Sanjuan, Juan Carlos

Published
2019
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5. Encuesta a los jefes de Unidad y residentes de Cirugía sobre la accesibilidad y el trabajo en los equipos de trasplante hepático en España

Author

López-Guerra, Diego, Rodríguez-Laiz, Gonzalo, Villar-del-Moral, Jesús, Pardo Sánchez, Fernando, Santoyo, Julio, López Andújar, Rafael, Nuño, Javier, Sánchez Turrión, Víctor, González Pinto, Ignacio, Jiménez, Carlos, López Baena, José Ángel, Gómez Gutierrez, Manuel, Fabregat Prous, Joan, Pacheco Sánchez, David, Barrera, Manuel, García-Gil, Agustín, Ramírez, Pablo, Varo, Evaristo, Bilbao, Itxarone, Fondevila, Constantino, Rodríguez-Sanjuan, Juan Carlos, Blanco Fernández, Gerardo, Gómez Bravo, Miguel Ángel, Briceño Delgado, Javier, and Valdivieso López, Andrés

Published
2019
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6. Multicentric Study on Total Pancreatectomies

Author

Fabregat Prous, Joan, Secanella, Lluis, Larrea, Javier, Olea, Sanchez Bueno, Francisco, Botello Martinez, Francisco, Briceño, Javier, Miyar-de León, Alberto, Serradilla, Mario, Serrablo, Alejandro, Ferrer Fabrega, Joana, Sanchez Cabús, S., Angel Gómez Bravo, Miguel, Padillo, Javier, Blanco, Laia, Balcells, J., Cugat, Esteban, García Domingo, Maribel, Muñoz Bellvis, Luis, Dolores Perez Diaz, Maria, Santoyo Santoyo, Julio, Sanchez, Belinda, Villegas, Trinidad, Pacho, Silvino, Díez Valladares, Luis, Rebollar, Jose, Ángel Suárez Muñoz, Miguel, Domínguez, Elías, Martín Perez, Elena, Falgueras, Laia, Poves, Ignasi, Artigas, Vicenç, Sabater, Luis, Garcia Gil, Agustin, Ignacio Miota de Llama, Jose, Manzanet, Gerardo, Carlos Pino, Jose, Carlos Rodríguez Sanjuán, Juan, Lluis, F., Ausania, Fabio, Alkorta Zuloaga, Maialen, Escartín, Jorge, Salas, Manel, Domingo, Carlos, Artigues Sánchez de Rojas, Enrique, Antonio Barreras Mateos, José, María Fernández Cebrián, José, Pérez Cabrera, Beatriz, Padilla Valverde, D., Sanjuanbenito, Alfonso, Iturburu Belmonte, Ignacio, Bejarano, Natalia, García Borobia, F., Toral Guinea, Pablo, Lopez Marcano, Aylhin, Asencio Arana, Francisco, Varo, Evaristo, Esteban, Rafael, Blas, Juan L., Jover Navalón, José M., Fernández Martínez, Cristina, Daban Collado, Enrique, Calvo Duran, Antonio, Vicens, J.C., Romero, J., Badía, J.M., Sánchez, Raquel, de Miguel Ibáñez, Ricardo, Pardoc, Fernando, Francos von Hunefeld, Carlos, Pereira, Fernando, Garcia Molina, Francisco, Rodríguez Prieto, Ignacio, Alonso Poza, Alfredo, Gilsanz, Carlos, Miguel Martínez Albert, Jose, Angel Morcillo, Miguel, Martínez Cortijo, Sagrario, Martín Fernández, José, Baquedano, Jesús, Castell, José, Aguiló, Javier, Carlos Bernal, Juan, Ramia, Jose M., Martin-Perez, Elena, Fabregat-Prous, Joan, Larrea y Olea, Javier, Sanchez-Bueno, Francisco, Botello-Martinez, Francisco, and Moya-Herraiz, Angel

Published
2019
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7. Estudio multicéntrico nacional sobre pancreatectomías totales

Author

Fabregat Prous, Joan, Secanella, Lluis, Larrea y Olea, Javier, Sanchez Bueno, Francisco, Botello Martinez, Francisco, Briceño, Javier, Miyar-de León, Alberto, Serradilla, Mario, Serrablo, Alejandro, Ferrer Fabrega, Joana, Sanchez Cabús, S., Angel Gómez Bravo, Miguel, Padillo, Javier, Blanco, Laia, Balcells, J., Cugat, Esteban, García Domingo, Maribel, Muñoz Bellvis, Luis, Dolores Perez Diaz, Maria, Santoyo Santoyo, Julio, Sanchez, Belinda, Villegas, Trinidad, Pacho, Silvino, Díez Valladares, Luis, Rebollar, Jose, Ángel Suárez Muñoz, Miguel, Domínguez, Elías, Martín Perez, Elena, Falgueras, Laia, Poves, Ignasi, Artigas, Vicenç, Sabater, Luis, Garcia Gil, Agustin, Ignacio Miota de Llama, Jose, Manzanet, Gerardo, Carlos Pino, Jose, Carlos Rodríguez Sanjuán, Juan, Lluis, F., Ausania, Fabio, Alkorta Zuloaga, Maialen, Escartín, Jorge, Salas, Manel, Domingo, Carlos, Artigues Sánchez de Rojas, Enrique, Antonio Barreras Mateos, José, María Fernández Cebrián, José, Pérez Cabrera, Beatriz, Padilla Valverde, D., Sanjuanbenito, Alfonso, Iturburu Belmonte, Ignacio, Bejarano, Natalia, García Borobia, F., Toral Guinea, Pablo, Lopez Marcano, Aylhin, Asencio Arana, Francisco, Varo, Evaristo, Esteban, Rafael, Blas, Juan Laaa, Jover Navalón, José M., Fernández Martínez, Cristina, Daban Collado, Enrique, Calvo Duran, Antonio, Vicens, J.C., Romero, J., Badía, J.M., Sánchez, Raquel, de Miguel Ibáñez, Ricardo, Pardoc, Fernando, Francos von Hunefeld, Carlos, Pereira, Fernando, Garcia Molina, Francisco, Rodríguez Prieto, Ignacio, Alonso Poza, Alfredo, Gilsanz, Carlos, Miguel Martínez Albert, Jose, Angel Morcillo, Miguel, Martínez Cortijo, Sagrario, Martín Fernández, José, Baquedano, Jesús, Castell, José, Aguiló, Javier, Carlos Bernal, Juan, Ramia, Jose M., Martin-Perez, Elena, Fabregat-Prous, Joan, Sanchez-Bueno, Francisco, Botello-Martinez, Francisco, and Moya-Herraiz, Angel

Published
2019
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11. Resultados de una encuesta sobre el soporte nutricional perioperatorio en la cirugía pancreática y biliar en España

Author

Loinaz, Carmelo, Ochando, Federico, Vicente, Emilio, Serrablo, Alejandro, López Cillero, Pablo, Gómez, Miguel Ángel, Fabregat Prous, Joan, Varo, Evaristo, Miyar de Leon, Albert, Fondevila, Constantino, Valdivieso, Andrés, Blanco, Gerardo, Sánchez, Belinda, López Andújar, Rafael, Fundora, Yilian, Cugat Andorrà, Esteban, Díez Valladares, Luis, Herrera, Javier, García Gil, Agustín, Morales, Rafael, Pardo, Fernando, Sabater, Luis, Baena, José Ángel, Muñoz Bellvis, Luis, Martín Pérez, Elena, Pérez Saborido, Baltasar, Suárez, Miguel Ángel, Meneu, Juan Carlos, Albiol, Maite, Sanjuanbenito, Alfonso, Ramia, José Miguel, Pereira, Fernando, Paseiro, Gloria, Palomo, Juan Carlos, Leon, Miguel, GENPOCIRP (Grupo Encuesta Nutrición PeriOperatoria en CIRugia Pancreatobiliar), UAM. Departamento de Cirugía, and Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (IIS-IP)

Subjects
medicine.medical_specialty, Medicina, Medicine (miscellaneous), Páncreas. Biliar. Cirugía. Nutrición, Nutritional Status, Critical Care and Intensive Care Medicine, Pancreatic head, Biliary surgery, Pancreatic surgery, Pancreatectomy, Surveys and Questionnaires, medicine, Humans, Malalties del pàncrees, Pancreas, Nutrició, Nutrition, Nutrition and Dietetics, Nutritional Support, business.industry, General surgery, Biliary, Guideline, Perioperative, Middle Aged, Biliar, Pancréas diseases, Biliary Tract Surgical Procedures, Páncreas, Solid food, Spain, Nutrición, Surgery, Preoperative fasting, business, Oral feeding
Abstract

Introduction: a survey on peri-operative nutritional support in pancreatic and biliary surgery among Spanish hospitals in 2007 showed that few surgical groups followed the 2006 ESPEN guidelines. Ten years later we sent a questionnaire to check the current situation. Methods: a questionnaire with 21 items sent to 38 centers, related to fasting time before and after surgery, nutritional screening use and type, time and type of peri-operative nutritional support, and number of procedures. Results: thirty-four institutions responded. The median number of pancreatic resections (head/total) was 29.5 (95% CI: 23.0-35; range, 5-68) (total, 1002); of surgeries for biliary malignancies (non-pancreatic), 9.8 (95% CI: 7.3-12.4; range, 2-30); and of main biliary resections for benign conditions, 10.4 (95% CI: 7.6-13.3; range, 2-33). Before surgery, only 41.2% of the sites used nutritional support (< 50% used any nutritional screening procedure). The mean duration of preoperative fasting for solid foods was 9.3 h (range, 6-24 h); it was 6.6 h for liquids (range, 2-12). Following pancreatic surgery, 29.4% tried to use early oral feeding, but 88.2% of the surveyed teams used some nutritional support; 26.5% of respondents used TPN in 100% of cases. Different percentages of TPN and EN were used in the other centers. In malignant biliary surgery, 22.6% used TPN always, and EN in 19.3% of cases. Conclusions: TPN is the commonest nutrition approach after pancreatic head surgery. Only 29.4% of the units used early oral feeding, and 32.3% used EN; 22.6% used TPN regularly after surgery for malignant biliary tumours. The 2006 ESPEN guideline recommendations are not regularly followed 12 years after their publication in our country., Introducción: realizamos una encuesta sobre soporte nutricional perioperatorio en cirugía pancreática y biliar en hospitales españoles en 2007, que mostró que pocos grupos quirúrgicos seguían las guías de ESPEN 2006. Diez años después enviamos un cuestionario para comprobar la situación actual. Métodos: treinta y ocho centros recibieron un cuestionario con 21 preguntas sobre tiempo de ayunas antes y después de la cirugía, cribado nutricional, duración y tipo de soporte nutricional perioperatorio, y número de procedimientos. Resultados: respondieron 34 grupos. La mediana de pancreatectomías (cabeza/total) fue de 29,5 (IC 95 %: 23,0-35; rango, 5-68) (total, 1002), la de cirugías biliares malignas de 9,8 (IC 95 %: 7,3-12,4; rango, 2-30) y la de resecciones biliares por patología benigna de 10,4 (IC 95 %: 7,6-13,3; rango, 2-33). Solo el 41,2 % de los grupos utilizaban soporte nutricional antes de la cirugía (< 50 % habian efectuado un cribado nutricional). El tiempo medio de ayuno preoperatorio para sólidos fue de 9,3 h (rango, 6-24 h), y de 6,6 h para líquidos (rango, 2-12). Tras la pancreatectomía, el 29,4 % habían intentado administrar una dieta oral precoz, pero el 88,2 % de los grupos usaron algún tipo de soporte nutricional y el 26,5 % usaron NP en el 100 % de los casos. Los demás grupos usaron diferentes porcentajes de NP y NE en sus casos. En la cirugía biliar maligna, el 22,6 % utilizaron NP siempre y NE en el 19,3 % de los casos. Conclusiones: la NP es el soporte nutricional más utilizado tras la cirugía de cabeza pancreática. Solo el 29,4 % de las unidades usan nutrición oral precoz y el 32,3 % emplean la NE tras este tipo de cirugía. El 22,6 % de las instituciones usan NP habitualmente tras la cirugía de tumores biliares malignos. Las guías ESPEN 2006 no se siguen de forma habitual en nuestro país tras más de 10 años desde su publicación.

Published
2020

13. Results of a survey on peri-operative nutritional support in pancreatic and biliary surgery in Spain

Author

Loinaz Segurola, Carmelo, primary, Ochando Cerdán, Federico, additional, Vicente López, Emilio, additional, Serrablo Requejo, Alejandro, additional, López Cillero, Pedro, additional, Gómez Bravo, Miguel Ángel, additional, Fabregat Prous, Juan, additional, Varo Pérez, Evaristo, additional, Miyar de León, Albert, additional, Fondevila Campo, Constantino, additional, Valdivieso López, Andrés, additional, Blanco Fernández, Gerardo, additional, Sánchez, Belinda, additional, López Andújar, Rafael, additional, Fundora Suárez, Yilian, additional, Cugat Andorra, Esteban, additional, Díez Valladares, Luis, additional, Herrera Cabezón, Javier, additional, García Gil, Agustín, additional, Morales Soriano, Rafael, additional, Pardo Sánchez, Fernando, additional, Sabater Ortí, Luis, additional, López Baena, José Ángel, additional, Muñoz Bellvís, Luis, additional, Martín Pérez, Elena, additional, Pérez Saborido, Baltasar, additional, Suárez Muñoz, Miguel Ángel, additional, Meneu Día, Juan Carlos, additional, Albiol Quer, Maite, additional, Sanjuanbenito Dehesa, Alfonso, additional, Ramia Ángel, José Ángel, additional, Pereira Pérez, Fernando, additional, Paseiro Crespo, Gloria, additional, Palomo Sánchez, Juan Carlos, additional, and León Sanz, Miguel, additional

Published
2020
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14. Encuesta a los jefes de Unidad y residentes de Cirugía sobre la accesibilidad y el trabajo en los equipos de trasplante hepático en España

Author

Blanco Fernández, Gerardo, primary, Gómez Bravo, Miguel Ángel, additional, Briceño Delgado, Javier, additional, Valdivieso López, Andrés, additional, López-Guerra, Diego, additional, Rodríguez-Laiz, Gonzalo, additional, Villar-del-Moral, Jesús, additional, Pardo Sánchez, Fernando, additional, Santoyo, Julio, additional, López Andújar, Rafael, additional, Nuño, Javier, additional, Sánchez Turrión, Víctor, additional, González Pinto, Ignacio, additional, Jiménez, Carlos, additional, López Baena, José Ángel, additional, Gómez Gutierrez, Manuel, additional, Fabregat Prous, Joan, additional, Pacheco Sánchez, David, additional, Barrera, Manuel, additional, García-Gil, Agustín, additional, Ramírez, Pablo, additional, Varo, Evaristo, additional, Bilbao, Itxarone, additional, Fondevila, Constantino, additional, and Rodríguez-Sanjuan, Juan Carlos, additional

Published
2019
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16. Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal

Author

Instituto de Salud Carlos III, European Commission, Zaouali, Mohamed A., Panisello-Roselló, Arnau, López, Alexandre, Castro-Benítez, Carlos, Folch-Puy, Emma, García-Gil, Agustín, Carbonell, Teresa, Adam, René, Roselló-Catafau, Joan, Instituto de Salud Carlos III, European Commission, Zaouali, Mohamed A., Panisello-Roselló, Arnau, López, Alexandre, Castro-Benítez, Carlos, Folch-Puy, Emma, García-Gil, Agustín, Carbonell, Teresa, Adam, René, and Roselló-Catafau, Joan

Abstract

[AIM] To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) solutions, [METHODS] Fatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 ℃ and subjected to “ex vivo ” normo-thermic perfusion (2 h; 37 ℃). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3)., [RESULTS] Profiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (p < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels., [CONCLUSION] Our comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis.

Published
2017

17. How important is donor age in liver transplantation?

Author

Lue, Alberto, Solanas, Estela, Baptista, Pedro, Lorente, Sara, Araiz, Juan José, García-Gil, Agustín, and Serrano, María Trinidad

Abstract

The age of liver donors has been increasing in the past several years because of a donor shortage. In the United States, 33% of donors are age 50 years or older, as are more than 50% in some European countries. The impact of donor age on liver transplantation (LT) has been analyzed in several studies with contradictory conclusions. Nevertheless, recent analyses of the largest databases demonstrate that having an older donor is a risk factor for graft failure. Donor age is included as a risk factor in the more relevant graft survival scores, such as the Donor Risk Index, donor age and Model for End-stage Liver Disease, Survival Outcomes Following Liver Transplantation, and the Balance of Risk. The use of old donors is related to an increased rate of biliary complications and hepatitis C virus-related graft failure. Although liver function does not seem to be significantly affected by age, the incidence of several liver diseases increases with age, and the capacity of the liver to manage or overcome liver diseases or external injuries decreases. In this paper, the importance of age in LT outcomes, the role of donor age as a risk factor, and the influence of aging on liver regeneration are reviewed.

Published
2016

18. Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1vsUniversity of Wisconsin appraisal

Author

Zaouali, Mohamed Amine, primary, Panisello-Roselló, Arnau, additional, Lopez, Alexandre, additional, Castro Benítez, Carlos, additional, Folch-Puy, Emma, additional, García-Gil, Agustín, additional, Carbonell, Teresa, additional, Adam, René, additional, and Roselló-Catafau, Joan, additional

Published
2017
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19. Polyethylene glycols: An effective strategy for limiting liver ischemia reperfusion injury

Author

Ministerio de Economía y Competitividad (España), Pasut, Gianfranco, Panisello-Roselló, Arnau, Folch-Puy, Emma, López, Alexandre, Castro-Benítez, Carlos, Calvo, Maria, Carbonell, Teresa, García-Gil, Agustín, Adam, René, Roselló-Catafau, Joan, Ministerio de Economía y Competitividad (España), Pasut, Gianfranco, Panisello-Roselló, Arnau, Folch-Puy, Emma, López, Alexandre, Castro-Benítez, Carlos, Calvo, Maria, Carbonell, Teresa, García-Gil, Agustín, Adam, René, and Roselló-Catafau, Joan

Abstract

Liver ischemia-reperfusion injury (IRI) is an inherent feature of liver surgery and liver transplantation in which damage to a hypoxic organ (ischemia) is exacerbated following the return of oxygen delivery (reperfusion). IRI is a major cause of primary nonfunction after transplantation and may lead to graft rejection, regardless of immunological considerations. The immediate response involves the disruption of cellular mitochondrial oxidative phosphorylation and the accumulation of metabolic intermediates during the ischemic period, and oxidative stress during blood flow restoration. Moreover, a complex cascade of inflammatory mediators is generated during reperfusion, contributing to the extension of the damage and finally to organ failure. A variety of pharmacological interventions (antioxidants, anticytokines, etc. ) have been proposed to alleviate graft injury but their usefulness is limited by the local and specific action of the drugs and by their potential undesirable toxic effects. Polyethylene glycols (PEGs), which are non-toxic water-soluble compounds approved by the FDA, have been widely used as a vehicle or a base in food, cosmetics and pharmaceuticals, and also as adjuvants for ameliorating drug pharmacokinetics. Some PEGs are also currently used as additives in organ preservation solutions prior to transplantation in order to limit the damage associated with cold ischemia reperfusion. More recently, the administration of PEGs of different molecular weights by intravenous injection has emerged as a new therapeutic tool to protect liver grafts from IRI. In this review, we summarize the current knowledge concerning the use of PEGs as a useful target for limiting liver IRI.

Published
2016

21. Emerging concepts in liver graft preservation

Author

Bejaoui, Mohamed, Pantazi, Eirini, Folch-Puy, Emma, Baptista, Pedro M., García-Gil, Agustín, Adam, René, Roselló-Catafau, Joan, Bejaoui, Mohamed, Pantazi, Eirini, Folch-Puy, Emma, Baptista, Pedro M., García-Gil, Agustín, Adam, René, and Roselló-Catafau, Joan

Abstract

The urgent need to expand the donor pool in order to attend to the growing demand for liver transplantation has obliged physicians to consider the use of suboptimal liver grafts and also to redefine the preservation strategies. This review examines the different methods of liver graft preservation, focusing on the latest advances in both static cold storage and machine perfusion (MP). The new strategies for static cold storage are mainly designed to increase the fatty liver graft preservation via the supplementation of commercial organ preservation solutions with additives. In this paper we stress the importance of carrying out effective graft washout after static cold preservation, and present a detailed discussion of the future perspectives for dynamic graft preservation using MP at different temperatures (hypothermia at 4 °C, normothermia at 37 °C and subnormothermia at 20 °C-25 °C). Finally, we highlight some emerging applications of regenerative medicine in liver graft preservation. In conclusion, this review discusses the “state of the art” and future perspectives in static and dynamic liver graft preservation in order to improve graft viability.

Published
2015

25. Corrigendum to “Immunosuppression without steroids in liver transplantation is safe and reduces infection and metabolic complications: Results from a prospective multicenter randomized study” [J Hepatol 44 (2006) 710–716]

Author

Llado, Laura, Xiol, Xavier, Figueras, Joan, Ramos, Emilio, Memba, Roberto, Serrano, Teresa, Torras, Jaume, Garcia-Gil, Agustin, Gonzalez-Pinto, Ignacio, Castellote, Jose, Baliellas, Carme, Fabregat, Joan, and Rafecas, Antoni

Published
2006
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26. Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine nonalcoholic steatohepatitis.

Author

Herrera-Marcos LV, Martínez-Beamonte R, Arnal C, Barranquero C, Puente-Lanzarote JJ, Lou-Bonafonte JM, Gonzalo-Romeo G, Mocciaro G, Jenkins B, Surra JC, Rodríguez-Yoldi MJ, Alastrué-Vera V, Letosa J, García-Gil A, Güemes A, Koulman A, and Osada J

Subjects
Swine, Animals, Lipidomics, Liver metabolism, Phospholipids metabolism, Cholesterol metabolism, Disease Models, Animal, Non-alcoholic Fatty Liver Disease metabolism
Abstract

Recently, the development of nonalcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol, and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using liquid chromatography-mass spectrometry analyzed 467 lipid species. Seven hepatic phospholipids [PC(30:0), PC(32:0), PC(33:0), PC(33:1), PC(34:0), PC(34:3) and PC(36:2)] significantly discriminated the time of dietary exposure, and PC(30:0), PC(33:0), PC(33:1) and PC(34:0) showed rapid adaptation in the reversion period. Three transcripts ( CS , MAT1A , and SPP1 ) showed significant changes associated with hepatic triglycerides and PC(33:0). Plasma lipidomics revealed that these species [FA 16:0, FA 18:0, LPC(17:1), PA(40:5), PC(37:1), TG(45:0), TG(47:2) and TG(51:0)] were able to discriminate the time of dietary exposure. Among them, FA 16:0, FA 18:0, LPC(17:1) and PA(40:5) changed the trend in the reversion phase. Plasma LDL-cholesterol and IL12P40 were good parameters to study the progression of NASH, but their capacity was surpassed by hepatic [PC(33:0), PC(33:1), and PC(34:0)] or plasma lipid [FA 16:0, FA 18:0, and LPC(17:1)] species. Taken together, these lipid species can be used as biomarkers of metabolic changes in the progression and regression of NASH in this model. The lipid changes suggest that the development of NASH also affects peripheral lipid metabolism. NEW & NOTEWORTHY A NASH stage was obtained in crossbred pigs. Hepatic [PC(33:0), PC(33:1) and PC(34:0)] or plasma [FA 16:0, FA 18:0 and LPC(17:1)] species were sensitive parameters to detect subtle changes in development and regression of nonalcoholic steatohepatitis (NASH). These findings may delineate the liquid biopsy to detect subtle changes in progression or in treatments. Furthermore, phospholipid changes according to the insult-inducing NASH may play an important role in accepting or rejecting fatty livers in transplantation.

Published
2024
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27. Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal.

Author

Zaouali MA, Panisello-Roselló A, Lopez A, Castro Benítez C, Folch-Puy E, García-Gil A, Carbonell T, Adam R, and Roselló-Catafau J

Subjects
Adenosine chemistry, Allopurinol chemistry, Animals, Apoptosis, Autophagy, Chromatography, High Pressure Liquid, Chymotrypsin chemistry, Glutathione chemistry, Homozygote, Inflammation, Insulin chemistry, Liver surgery, Male, Mitochondria pathology, Organ Preservation methods, Perfusion, Proteolysis, Raffinose chemistry, Rats, Rats, Zucker, Fatty Liver surgery, Graft Survival, Liver Transplantation methods, Organ Preservation Solutions chemistry, Proteasome Endopeptidase Complex metabolism
Abstract

Aim: To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) solutions., Methods: Fatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °Cand subjected to " ex vivo " normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3)., Results: Profiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW ( P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels., Conclusion: Our comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis., Competing Interests: Conflict-of-interest statement: None of the authors have any conflict of interest to declare related to this paper.

Published
2017
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28. Emerging concepts in liver graft preservation.

Author

Bejaoui M, Pantazi E, Folch-Puy E, Baptista PM, García-Gil A, Adam R, and Roselló-Catafau J

Subjects
Animals, Humans, Liver pathology, Liver Transplantation adverse effects, Organ Preservation adverse effects, Organ Preservation Solutions adverse effects, Perfusion adverse effects, Tissue Survival, Cold Temperature adverse effects, Liver drug effects, Liver surgery, Liver Transplantation methods, Organ Preservation methods, Organ Preservation Solutions therapeutic use, Perfusion methods, Regenerative Medicine methods
Abstract

The urgent need to expand the donor pool in order to attend to the growing demand for liver transplantation has obliged physicians to consider the use of suboptimal liver grafts and also to redefine the preservation strategies. This review examines the different methods of liver graft preservation, focusing on the latest advances in both static cold storage and machine perfusion (MP). The new strategies for static cold storage are mainly designed to increase the fatty liver graft preservation via the supplementation of commercial organ preservation solutions with additives. In this paper we stress the importance of carrying out effective graft washout after static cold preservation, and present a detailed discussion of the future perspectives for dynamic graft preservation using MP at different temperatures (hypothermia at 4 °C, normothermia at 37 °C and subnormothermia at 20 °C-25 °C). Finally, we highlight some emerging applications of regenerative medicine in liver graft preservation. In conclusion, this review discusses the "state of the art" and future perspectives in static and dynamic liver graft preservation in order to improve graft viability.

Published
2015
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