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86 results on '"García-Mendiola T"'

2. Pathogen sensing device based on 2D MoS2/graphene heterostructure.

Author

Enebral-Romero E., Gutiérrez-Gálvez L., Del Caño R., Sulleiro M.V., Naranjo A., Gómez I.J., Pariente F., Pérez, Emilio M., García-Mendiola T., Lorenzo E., Enebral-Romero E., Gutiérrez-Gálvez L., Del Caño R., Sulleiro M.V., Naranjo A., Gómez I.J., Pariente F., Pérez, Emilio M., García-Mendiola T., and Lorenzo E.

Published
2023

6. Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

Author

Gutiérrez-Gálvez L., del Caño R., Menéndez-Luque I., García-Nieto D., Rodríguez-Peña M., Luna M., Pineda T., Pariente F., García-Mendiola T., Lorenzo E., Gutiérrez-Gálvez L., del Caño R., Menéndez-Luque I., García-Nieto D., Rodríguez-Peña M., Luna M., Pineda T., Pariente F., García-Mendiola T., and Lorenzo E.

Published
2022

7. Neutral Red-carbon nanodots for selective fluorescent DNA sensing

Author

Martínez-Periñán E., Martínez-Sobrino Á., Bravo I., García-Mendiola T., Mateo-Martí E., Pariente F., Lorenzo E., Martínez-Periñán E., Martínez-Sobrino Á., Bravo I., García-Mendiola T., Mateo-Martí E., Pariente F., and Lorenzo E.

Published
2022

9. Paving the way to point of care (POC) devices for SARS-CoV-2 detection

Author

Kaci K., Del Caño R., Luna M., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Somoza, Álvaro, Miranda, Rodolfo, González de Rivera G., García-Mendiola T., Lorenzo E., Kaci K., Del Caño R., Luna M., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Somoza, Álvaro, Miranda, Rodolfo, González de Rivera G., García-Mendiola T., and Lorenzo E.

Published
2022

10. Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides

Author

del Caño R., García-Mendiola T., García-Nieto D., Álvaro R., Luna M., Iniesta H.A., Coloma R., Diaz C.R., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Pineda T., Pariente F., Miranda, Rodolfo, Somoza, Álvaro, Lorenzo E., del Caño R., García-Mendiola T., García-Nieto D., Álvaro R., Luna M., Iniesta H.A., Coloma R., Diaz C.R., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Pineda T., Pariente F., Miranda, Rodolfo, Somoza, Álvaro, and Lorenzo E.

Published
2022

11. Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing

Author

Pina-Coronado C., Martínez-Sobrino Á., Gutiérrez-Gálvez L., Del Caño R., Martínez-Periñán E., García-Nieto D., Rodríguez-Peña M., Luna M., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Pineda T., Pariente F., Somoza, Álvaro, García-Mendiola T., Miranda, Rodolfo, Lorenzo E., Pina-Coronado C., Martínez-Sobrino Á., Gutiérrez-Gálvez L., Del Caño R., Martínez-Periñán E., García-Nieto D., Rodríguez-Peña M., Luna M., Milán-Rois P., Castellanos M., Abreu M., Cantón R., Galán J.C., Pineda T., Pariente F., Somoza, Álvaro, García-Mendiola T., Miranda, Rodolfo, and Lorenzo E.

Published
2022

13. A MoS2 platform and thionine-carbon nanodots for sensitive and selective detection of pathogens

Author

Martínez-Periñán E., García-Mendiola T., Enebral-Romero E., del Caño R., Vera-Hidalgo M., Vázquez Sulleiro M., Navío C., Pariente F., Pérez, Emilio M., Lorenzo E., Martínez-Periñán E., García-Mendiola T., Enebral-Romero E., del Caño R., Vera-Hidalgo M., Vázquez Sulleiro M., Navío C., Pariente F., Pérez, Emilio M., and Lorenzo E.

Published
2021

14. Carbon nanodot–based electrogenerated chemiluminescence biosensor for miRNA-21 detection

Author

Gutiérrez-Gálvez L., García-Mendiola T., Gutiérrez-Sánchez C., Guerrero-Esteban T., García-Diego C., Buendía I., García-Bermejo M.L., Pariente F., Lorenzo E., Gutiérrez-Gálvez L., García-Mendiola T., Gutiérrez-Sánchez C., Guerrero-Esteban T., García-Diego C., Buendía I., García-Bermejo M.L., Pariente F., and Lorenzo E.

Published
2021

16. Biosensors

Author

Martínez-Periñán E., Gutiérrez-Sánchez C., García-Mendiola T., Lorenzo E. and This research was founded by the Ministerio de Ciencia, Innovaci?n, Universidades of Spain (CTQ2017-84309-C2-1-R and RED2018-102412-T), Comunidad Aut?noma de Madrid (TRANSNANOAVANSENS Program). C.G.-S. also acknowledges the financial support from the Comunidad Aut?noma de Madrid, Atracci?n de Talento Program (2017-T1/BIO-5435).

Published
2020

19. ZnO nanowire-based fluorometric enzymatic assays for lactate and cholesterol

Author

Briones M., Busó-Rogero C., Catalán-Gómez S., García-Mendiola T., Pariente F., Redondo-Cubero A., Lorenzo M.E. and The financial supports from the Spanish Ministerio de Ciencia, Innovación y Universidades (CTQ2017-84309-C2-1-R and CTQ2017-84309-C2-2-R) and Comunidad Autónoma de Madrid (TRANSNANOAVANSENS Program. S2018/NMT-4349) are gratefully acknowledged. IMDEA Nanociencia acknowledges support from the ‘Severo Ochoa’ Programme for Centres of Excellence in R&D (Ministerio de Ciencia, Innovación y Universidades, Grant SEV-2016-0686). C. B.-R. acknowledges the postdoctoral fellowship APOSTD/2017/010 from the Generalitat Valenciana. A.R.C. acknowledges Ramón y Cajal program (under contract number RYC-2015-18047). Authors wish to thank Centro de Biologia Molecular for microscopy fluorescence measurements.

Published
2020

21. Functionalization of a Few-Layer Antimonene with Oligonucleotides for DNA Sensing

Author

García-Mendiola T., Gutiérrez-Sánchez C., Gibaja C., Torres I., Busó-Rogero C., Pariente F., Solera J., Razavifar Z., Palacios J.J., Zamora F., Lorenzo E., García-Mendiola T., Gutiérrez-Sánchez C., Gibaja C., Torres I., Busó-Rogero C., Pariente F., Solera J., Razavifar Z., Palacios J.J., Zamora F., and Lorenzo E.

Published
2020

23. Enhanced performance of reagent-less carbon nanodots based enzyme electrochemical biosensors

Author

Bravo I., Gutiérrez-Sánchez C., García-Mendiola T., Revenga-Parra M., Pariente F., Lorenzo E. and 'The authors acknowledge the Spanish Ministerio de Econom?a, Industria y Competitividad through project CTQ2017-84309-C2-1-R',' Ministerio de Ciencia, Innovaci?n y Universidades through project RED2018-102412-T',' and Comunidad de Madrid through project S2018/NMT-4349 (TRANSNANOAVANSENS) for financial support.'

Published
2019

26. Electrochemically driven phenothiazine modification of carbon nanodots

Author

Mediavilla M., Martínez-Periñán E., Bravo I., García-Mendiola T., Revenga-Parra M., Pariente F., Lorenzo E. and The authors acknowledge the Ministry of Economy and Competitiveness (project No. CTQ2014-53334-C2-1-R, CTQ2017-84309-C2-1-R and CTQ2015-71955-REDT) and Community of Madrid (NANOAVANSENS Program) for financial support.

Published
2018

27. Metallacarboranes on the Road to Anticancer Therapies: Cellular Uptake, DNA Interaction, and Biological Evaluation of Cobaltabisdicarbollide [COSAN]−

Author

Fuentes I., García-Mendiola T., Sato S., Pita M., Nakamura H., Lorenzo E., Teixidor F., Marques F., Viñas C. and This work has been supported by the Spanish Ministerio de Economía y Competitividad (projects: CTQ2016-75150-R, CTQ2015-71955-REDT (ELECTROBIONET) and CTQ2014-53334-C2-1-R), Generalitat de Catalunya (2017 SGR 1720), by the Comunidad Autónoma de Madrid NANOAVANSENS (S2013/MIT-3029), and a Grant-in-Aid for Scientific Research (B) (17H02202) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. C2TN/IST author gratefully acknowledges the FCT support through the UID/Multi/04349/2013 project. I.F. is enrolled in the PhD program of UAB.

Published
2018

28. Carbon nanodots based biosensors for gene mutation detection

Author

García-Mendiola T., Bravo I., López-Moreno J.M., Pariente F., Wannemacher R., Weber K., Popp J., Lorenzo E. and This work has been supported by the Comunidad Autónoma de Madrid NANOAVANSENS (project No. S2013/MIT-3029) and Spanish Ministerio de Economía, Industria y Competitividad through projects Nos. CTQ2015-71955-REDT (ELECTROBIONET) and CTQ2014-53334-C2-1-R. E. L. thanks the Fulbright scholarship-Salvador de Madariaga program from Spanish Ministerio de Economía, Industria y Competitividad. I. B. gratefully acknowledges the FPI-2012 Grant from Spanish Ministerio de Economía y Competitividad . R.W. gratefully acknowledges support by the European Union structural funds and the Comunidad de Madrid MAD2D-CM Program (S2013/MIT-3007), as well as by the Spanish Ministerio de Economía, Industria y Competitividad through project No. MAT2015-71879-P.

Published
2018

29. Carbon nanodots based biosensors for gene mutation detection

Author

García-Mendiola T., Bravo I., López-Moreno J.M., Pariente F., Wannemacher, Reinhold, Weber K., Popp J., Lorenzo E., García-Mendiola T., Bravo I., López-Moreno J.M., Pariente F., Wannemacher, Reinhold, Weber K., Popp J., and Lorenzo E.

Published
2018

32. Dyes as bifunctional markers of DNA hybridization on surfaces and mutation detection

Author

García-Mendiola T., Cerro M.R., López-Moreno J.M., Pariente F., Lorenzo E. and This work has been supported by the Comunidad Autónoma de Madrid (project no. S2009/PPQ-363 1642 ), NANOAVANSENS (project no. S2013/MIT-3029 ), the Spanish Ministerio de Economía y Competitividad (project nos. CTQ2015-71955-REDT (ELECTROBIONET), CTQ2014-53334-C2-1-R and CTQ2013-44670-R ). E. Lorenzo thanks the Fulbright scholarship-Salvador de Madariaga program from Spanish Ministerio de Economía y Competitividad.

Published
2016

35. Diazonium salt click chemistry based multiwall carbon nanotube electrocatalytic platforms

Author

Bravo I., García-Mendiola T., Revenga-Parra M., Pariente F., Lorenzo E. and Authors greatly thank to M.A. Fernández and Dr. F. Zamora their kind help with the experimental and discussion of AFM results. This work was supported by the MICINN and the Comunidad Autónoma de Madrid through the projects CTQ2011-28157 and S2009/PPQ-1642 , AVANSENS, respectively. IB gratefully appreciates the financial support from MINECO of Spain.

Published
2015

36. Scaffold electrodes based on thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and Azure A films for high performance biosensor

Author

Gómez-Anquela C., García-Mendiola T., Abad J.M., Pita M., Pariente F., Lorenzo E. and This work has been supported by the Comunidad Autonoma de Madrid ( NANOAVANSENS S2013/MIT 3029 ) and the Spanish Ministerio de Ciencia e Innovacion (project No. CTQ2011-28157 ).M. P. acknowledges the 2009 Ramon y Cajal program from the Spanish MINECO.

Published
2015

45. Simple diazonium chemistry to develop specific gene sensing platforms

Author

Revenga-Parra M., García-Mendiola T., González-Costas J., González-Romero E., Marín A.G., Pau J.L., Pariente F., Lorenzo E., Revenga-Parra M., García-Mendiola T., González-Costas J., González-Romero E., Marín A.G., Pau J.L., Pariente F., and Lorenzo E.

Published
2014

47. Compelling DNA intercalation through 'anion-anion' anti-coulombic interactions: boron cluster self-vehicles as promising anticancer agents.

Author

Gutiérrez-Gálvez L, García-Mendiola T, Lorenzo E, Nuez-Martinez M, Ocal C, Yan S, Teixidor F, Pinheiro T, Marques F, and Viñas C

Subjects
Humans, Boron Compounds chemistry, Boron Compounds pharmacology, Molecular Structure, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, DNA chemistry, Intercalating Agents chemistry, Intercalating Agents pharmacology, Anions chemistry
Abstract

Anticancer drugs inhibit DNA replication by intercalating between DNA base pairs, forming covalent bonds with nucleotide bases, or binding to the DNA groove. To develop safer drugs, novel molecular structures with alternative binding mechanisms are essential. Stable boron hydrides offer a promising alternative for cancer therapy, opening up additional options like boron neutron capture therapy based on 10 B and thermal neutron beams or proton boron fusion therapy using 11 B and proton beams. These therapies are more efficient when the boron compound is ideally located inside cancer cells, particularly in the nucleus. Current cancer treatments often utilize small, polycyclic, aromatic, planar molecules that intercalate between ds-DNA base pairs, requiring only a spacing of approximately 0.34 nm. In this paper, we demonstrate another type of intercalation. Notably, [3,3'-Fe(1,2-C 2 B 9 H 11 ) 2 ] - , ([ o -FESAN] - ), a compact 3D molecule measuring 1.1 nm × 0.6 nm, can as well intercalate by strong non-bonding interactions preferentially with guanine. Unlike known intercalators, which are positive or neutral, [ o -FESAN] - is a negative species and when an [ o -FESAN] - molecule approaches the negatively charged DNA phosphate chain an anion-anion interaction consistently anti-electrostatic via C cluster -H⋯O-P bonds occurs. Then, when more molecules approach, an elongated outstandingly self-assembled structure of [ o -FESAN] - -[ o -FESAN] - forms moving anions towards the interthread region to interact with base pairs and form aggregates of four [ o -FESAN] - anions per base pair. These aggregates, in this environment, are generated by C cluster -H⋯O-C, N-H⋯H-B and C cluster -H⋯H-B interactions. The ferrabis(dicarbollide) boron-rich small molecules not only effectively penetrate the nucleus but also intercalate with ds-DNA, making them promising for cancer treatment. This amphiphilic anionic molecule, used as a carrier-free drug, can enhance radiotherapy in a multimodal perspective, providing healthcare professionals with improved tools for cancer treatment. This work demonstrates these findings with a plethora of techniques.

Published
2024
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48. Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection.

Author

Enebral-Romero E, García-Fernández D, Gutiérrez-Gálvez L, López-Diego D, Luna M, García-Martín A, Salagre E, Michel EG, Torres Í, Zamora F, García-Mendiola T, and Lorenzo E

Subjects
Humans, Electrochemical Techniques methods, Nanostructures chemistry, DNA chemistry, Aptamers, Nucleotide chemistry, SARS-CoV-2 isolation & purification, Biosensing Techniques methods, Spike Glycoprotein, Coronavirus analysis, Spike Glycoprotein, Coronavirus chemistry, COVID-19 virology, COVID-19 diagnosis, Limit of Detection
Abstract

In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL -1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2024
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49. Functionalized N95 Face Mask with a Chemical-Free Paper-Based Collector for Exhaled Breath Analysis: SARS-CoV-2 Detection with a Printed Immunosensor as a Case Study.

Author

Gutiérrez-Gálvez L, Seddaoui N, Fiore L, Fabiani L, García-Mendiola T, Lorenzo E, and Arduini F

Subjects
Humans, Immunoassay instrumentation, Immunoassay methods, Limit of Detection, Electrochemical Techniques instrumentation, Electrochemical Techniques methods, Exhalation, N95 Respirators, Saliva chemistry, Saliva virology, SARS-CoV-2 immunology, SARS-CoV-2 isolation & purification, Breath Tests instrumentation, Breath Tests methods, Paper, COVID-19 diagnosis, COVID-19 virology, Biosensing Techniques instrumentation, Biosensing Techniques methods, Spike Glycoprotein, Coronavirus analysis, Spike Glycoprotein, Coronavirus immunology
Abstract

Exhaled breath electrochemical sensing is a promising biomedical technology owing to its portability, painlessness, cost-effectiveness, and user-friendliness. Here, we present a novel approach for target analysis in exhaled breath by integrating a comfortable paper-based collector into an N95 face mask, providing a universal solution for analyzing several biomarkers. As a model analyte, we detected SARS-CoV-2 spike protein from the exhaled breath by sampling the target analyte into the collector, followed by its detection out of the N95 face mask using a magnetic bead-based electrochemical immunosensor. This approach was designed to avoid any contact between humans and the chemicals. To simulate human exhaled breath, untreated saliva samples were nebulized on the paper collector, revealing a detection limit of 1 ng/mL and a wide linear range of 3.7-10,000 ng/mL. Additionally, the developed immunosensor exhibited high selectivity toward the SARS-CoV-2 spike protein, compared to other airborne microorganisms, and the SARS-CoV-2 nucleocapsid protein. Accuracy assessments were conducted by analyzing the simulated breath samples spiked with varying concentrations of SARS-CoV-2 spike protein, resulting in satisfactory recovery values (ranging from 97 ± 4 to 118 ± 1%). Finally, the paper-based hybrid immunosensor was successfully applied for the detection of SARS-CoV-2 in real human exhaled breath samples. The position of the collector in the N95 mask was evaluated as well as the ability of this paper-based analytical tool to identify the positive patient.

Published
2024
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50. Rapid and simple viral protein detection by functionalized 2D MoS 2 /graphene electrochemiluminescence aptasensor.

Author

Gutiérrez-Gálvez L, El Hajioui-El Ghalbzouri H, Enebral-Romero E, Garrido M, Naranjo A, López-Diego D, Luna M, Pérez EM, García-Mendiola T, and Lorenzo E

Subjects
Humans, Limit of Detection, COVID-19 diagnosis, COVID-19 virology, Electrodes, Saliva chemistry, Saliva virology, Graphite chemistry, Disulfides chemistry, Molybdenum chemistry, Aptamers, Nucleotide chemistry, Electrochemical Techniques methods, Biosensing Techniques methods, SARS-CoV-2 isolation & purification, SARS-CoV-2 immunology, Luminescent Measurements methods, Spike Glycoprotein, Coronavirus analysis
Abstract

In this work we present the development of an electrochemiluminescence aptasensor based on electrografting molybdenum disulphide nanosheets functionalized with diazonium salt (MoS 2 -N 2 + ) upon screen-printed electrodes of graphene (SPEs GPH) for viral proteins detection. In brief, this aptasensor consists of SPEs GPH electrografted with MoS 2 -N 2 + and modified with a thiolated aptamer, which can specifically recognize the target protein analyte. In this case, we have used SARS-CoV-2 spike protein as model protein. Electrochemiluminescence detection was performed by using the [Ru(bpy) 3 ] 2+ /TPRA (tripropylamine) system, which allows the specific detection of the SARS-CoV-2 spike protein easily and rapidly with a detection limit of 9.74 fg/mL and a linear range from 32.5 fg/mL to 50.0 pg/mL. Moreover, the applicability of the aptasensor has been confirmed by the detection of the protein directly in human saliva samples. Comparing our device with a traditional saliva antigen test, our aptasensor can detect the spike protein even when the saliva antigen test gives a negative result., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2024
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