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27 results on '"Gao, Yudan"'

11. Activation of endogenous neurogenesis and angiogenesis by basic fibroblast growth factor-chitosan gel in an adult rat model of ischemic stroke

Author

Duan, Hongmei, primary, Li, Shulun, additional, Hao, Peng, additional, Hao, Fei, additional, Zhao, Wen, additional, Gao, Yudan, additional, Qiao, Hui, additional, Gu, Yiming, additional, Lv, Yang, additional, Bao, Xinjie, additional, Chiu, Kin, additional, So, Kwok-Fai, additional, Yang, Zhaoyang, additional, and Li, Xiaoguang, additional

Published
2023
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18. Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System

Author

Li, Manli, Zhao, Wen, Gao, Yudan, Hao, Peng, Shang, Junkui, Duan, Hongmei, Yang, Zhaoyang, and Li, Xiaoguang

Subjects
MAPK/ERK pathway, Article Subject, lcsh:Medicine, Bone Marrow Cells, Fibroblast growth factor, General Biochemistry, Genetics and Molecular Biology, Immediate-Early Proteins, Neural Stem Cells, Animals, Cell Lineage, Inducer, Receptor, Fibroblast Growth Factor, Type 1, Receptor, Cell Proliferation, Neurons, Regulation of gene expression, Chitosan, Mitogen-Activated Protein Kinase 3, BTG2, General Immunology and Microbiology, Cell growth, Chemistry, Tumor Suppressor Proteins, Fibroblast growth factor receptor 1, lcsh:R, Gene Expression Regulation, Developmental, Cell Differentiation, Mesenchymal Stem Cells, General Medicine, Rats, Cell biology, Fibroblast Growth Factor 2, Proto-Oncogene Proteins c-fos, Research Article
Abstract

Bone marrow mesenchymal stem cells undergo differentiation to different lineages with different efficiencies when induced by different factors. We added a bFGF-chitosan controlled release system (bFGF-CCRS) as an inducer into conditioned medium to facilitate the oriented differentiation of BMSCs into neural lineage cells (eventually mature neurons); furthermore, we synchronized BMSCs to the G0/G1 phase via serum starvation to observe the effect of the inducer on the differentiation direction and efficiency. The nonsynchronized group, chitosan alone (not loaded with bFGF) group, soluble bFGF group, and conditioned medium group served as controls, and we observed the dynamic process of differentiation of BMSCs into neural lineage cells at different time points after the beginning of coculture. We analyzed the binding patterns of bFGF and chitosan and assayed the expression differences of key factors (FGFR1, ERK, and c-fos) and molecular switches (BTG2) that regulate the transformation from cell proliferation to differentiation. We also investigated the potential molecular mechanism of BMSC differentiation into neural lineage cells at a high percentage when induced by bFGF-CCRS.

Published
2019
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26. Activation of adult endogenous neurogenesis by a hyaluronic acid collagen gel containing basic fibroblast growth factor promotes remodeling and functional recovery of the injured cerebral cortex.

Author

Li, Yan, Hao, Peng, Duan, Hongmei, Hao, Fei, Zhao, Wen, Gao, Yudan, Yang, Zhaoyang, So, Kwok-Fai, and Li, Xiaoguang

Abstract

The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury. However, whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions, such as the cortex, remains unknown. In this study, we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury. Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells, as well as their differentiation into mature and functionally integrated neurons. Importantly, these new neurons reconstructed the architecture of cortical layers II to VI, integrated into the existing neural circuitry, and ultimately led to improved brain function. These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries. [ABSTRACT FROM AUTHOR]

Published
2025
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27. Activation of endogenous neurogenesis and angiogenesis by basic fibroblast growth factor-chitosan gel in an adult rat model of ischemic stroke.

Author

Duan H, Li S, Hao P, Hao F, Zhao W, Gao Y, Qiao H, Gu Y, Lv Y, Bao X, Chiu K, So KF, Yang Z, and Li X

Abstract

Attempts have been made to use cell transplantation and biomaterials to promote cell proliferation, differentiation, migration, and survival, as well as angiogenesis, in the context of brain injury. However, whether bioactive materials can repair the damage caused by ischemic stroke by activating endogenous neurogenesis and angiogenesis is still unknown. In this study, we applied chitosan gel loaded with basic fibroblast growth factor to the stroke cavity 7 days after ischemic stroke in rats. The gel slowly released basic fibroblast growth factor, which improved the local microenvironment, activated endogenous neural stem/progenitor cells, and recruited these cells to migrate toward the penumbra and stroke cavity and subsequently differentiate into neurons, while enhancing angiogenesis in the penumbra and stroke cavity and ultimately leading to partial functional recovery. This study revealed the mechanism by which bioactive materials repair ischemic strokes, thus providing a new strategy for the clinical application of bioactive materials in the treatment of ischemic stroke., Competing Interests: None

Published
2024
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