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Your search keyword '"Gao, Xiaohang"' showing total 22 results
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22 results on '"Gao, Xiaohang"'

2. Improvement of Osteoporosis in Rats With Hind-Limb Unloading Treated With Pulsed Electromagnetic Field and Whole-Body Vibration

Author

Liu, Xifang, Gao, Xiaohang, Tong, Jie, Yu, Liyin, Xu, Minglong, and Zhang, Jianbao

Subjects
Osteoporosis -- Diagnosis -- Care and treatment, Electromagnetic fields -- Analysis, Animal experimentation -- Evaluation
Abstract

Objective. Physical factors have been used to address disuse osteoporosis, but their effects and mechanism remain unclear. The purpose of this study was to determine the effects of pulsed electromagnetic field (PEMF) and whole-body vibration (WBV) on disuse osteoporosis to increase knowledge about treating osteoporosis. Methods. A disuse osteoporosis rat model was developed by hind-limb unloading (HU) for 6 weeks. Forty 4-month-old female Sprague--Dawley rats were divided into 5 groups and given the following interventions: HU, HU treated with PEMF (HUP), HU treated with WBV (HUW), HU treated with both PEMF and WBV (HUPW), and no intervention (controls). After 8 weeks of intervention, measurements were taken. Results. HU induced a decrease in bone mineral density (BMD), whereas HUP HUW, and HUPW increased it. Moreover, the bone resorption markers tartrate-resistant acid phosphatase (TRAP) and C-terminal peptide of type 1 collagen in the HU group significantly increased, whereas the osteogenesis markers osteocalcin and N-terminal propeptide of type 1 procollagen significantly decreased. The markers osteocalcin and N-terminal propeptide of type 1 procollagen significantly increased, but TRAP and C-terminal peptide of type 1 collagen significantly decreased in the HUPW, HUP and HUW groups compared with the HU group. In particular, HUPW effectively increased osteocalcin and decreased TRAP compared with HUP and WBV. Microcomputed tomography analysis of the femur indicated that HUPW improved trabecular number, bone volume over total volume, bone surface over bone volume, trabecular separation, and the structure model index compared with HUP and that it improved bone surface over bone volume, trabecular separation, and structure model index compared with HUW. The HUPW group showed a significant increase in maximum load compared with the HUW group and a significant increase in elastic modulus compared with the HUP group. Conclusion. PEMF, WBV, and their combination all attenuated bone resorption and enhanced osteogenesis. WBV and the combination of treatments have great potential to improve osteogenesis compared with PEMF. In addition, HUPW significantly attenuated bone resorption compared with HUW and HUP. Impact. The results of this study indicated that HUPW could effectively improve disuse osteoporosis compared with HUP, given that trabecular number and bone volume over total volume are associated with disuse osteoporosis. Moreover, BMD recovered well with HUP HUW, and HUPW but the bone structure--especially mechanical performance--did not, indicating that osteoporosis should be evaluated with BMD and mechanical performance, not with BMD in isolation. Keywords: Disuse Osteoporosis Hind-Limb Unloading, Pulsed Electromagnetic Field, Rat, Whole-Body Vibration, Introduction Osteoporosis, characterized by a progressive loss of bone tissue and then a decrease in bone mineral density (BMD), remains a public health problem because it reduces the quality of [...]

Published
2022
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12. KIF2 mediates the neuroprotection in cerebral ischaemia injury by affecting NF‐κB pathway.

Author

Wang, Jin, Chen, Jie, Chen, Jun, Liu, Xifang, Yang, Haixia, Liu, Jing, He, Ali, Gao, Xiaohang, and Xin, Yinhu

Subjects
NF-kappa B, GLUTATHIONE peroxidase, CEREBROVASCULAR disease, SUPEROXIDE dismutase, WOUNDS & injuries, CEREBRAL arteries
Abstract

Stroke is the most common cerebrovascular disease with high morbidity and mortality around the world. However, the underlying mechanisms involved in nerve injury and cerebral ischaemia/reperfusion (I/R) during cerebrovascular disease are still not completely clear. In the present study, we investigate the role of kinesin family member 2 (KIF2) in the neuroprotection after cerebral I/R injury. KIF2 was aberrantly expressed in the cerebral tissues from middle cerebral artery occlusion (MCAO) rat model in a time dependent manner. A similar changing pattern was found in the cultured hypoxic neurons as well as SK‐N‐SH cells in vitro. Compared to the control, KIF2 inhibition significantly increased the level of malonic dialdehyde (MDA), and reduced the level of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH‐px) activity in cerebral tissues of MCAO rat model. The reactive oxygen species (ROS) level was also up‐regulated after KIF2 siRNA knockdown in cultured hypoxic SK‐N‐SH cells. The apoptosis rates of hypoxic neurons and SK‐N‐SH cells as well as activated‐caspase‐3 level were obviously increased after KIF2 silencing. Furthermore, we found that the nuclear factor‐kappa B (NF‐κB) pathway was involved in KIF2‐mediated neuroprotection after cerebral I/R injury, and induced apoptosis of hypoxic SK‐N‐SH cells by KIF2 silencing could be attenuated by the specific inhibitor BAY11‐7082 of NF‐κB. In conclusion, we demonstrate that KIF2 could mediate the neuroprotection in cerebral I/R injury by inhibiting activation of NF‐κB pathway. This might provide a novel therapeutic target for cerebral I/R injury. [ABSTRACT FROM AUTHOR]

Published
2020
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