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We have fabricated a microinductor with an ultralow profile by a microelectromechanical systems (MEMS) technique. The fabrication process uses UV-LIGA, dry etching, fine polishing, and electroplating to achieve high performance. The dimensions of the inductor are 1500 [micro]m x 900 [micro]m x 100 [micro]m. It has 41 turns, with coil width of 20 [micro]m, space of 20 [micro]m, and a high aspect ratio of 5:1. The inductance is 0.424 [micro]H and the quality factor (Q factor) is about 1.7 at a frequency of 1 MHz. The stray capacitance is approximately zero over the frequency range measured. Index Terms--Inductance, magnetic cores, microelectromechanical systems (MEMS), microinductors, quality factor.

Ischemic stroke is a major cause of death in the global population, with a high disability and mortality rate. Lack of regenerative ability is considered to be the fundamental cause. This study aims to determine the effect of Shh pathway, which mediates regenerative signaling in response to CNS injury, on myelin repair and Olig1 expression in focal ischemic lesions in the rat. A model of middle cerebral artery occlusion (MCAO) was established using the intraluminal suture method where the middle cerebral artery (MCA) was restricted for 120 min. Cyclopamine, a specific inhibitor of Shh, or saline was administered 12h after MCAO surgery and lasted for 7d. After MCA occlusion, male Sprague-Dawley rats were randomly allocated to cyclopamine- or saline-treated groups. A group of no-injection animals after MCAO were used as control. The Shh signaling pathway, myelinogenesis-related factor MBP and Olig1 were tested using immunohistochemistry and RT-PCR assay. The levels of Shh and its component Gli1 were elevated from 1d up to 14d following ischemia, indicating that the Shh-Gli1 axis was broadly reactivated. Treatment with cyclopamine can partially block the Shh signaling pathway, prevent myelin repair, and decrease the Olig1 expression following ischemic stroke. That blockade of Shh signaling concurrently with the creation of a lesion aggravated ischemic myelin damage, probably via its downstream effects on Olig1 transcription. Shh plays a contributory role during regeneration in the CNS, thereby providing promising new therapeutic strategies to assist in recovery from ischemic stroke. [ABSTRACT FROM AUTHOR]

Tropical cyclone (TC) rainfall forecast has remained a challenge. To create initial conditions with high quality for simulation, the present study implemented a data assimilation scheme based on the EnKF method to ingest the satellite-retrieved cloud water path (Cw) and tested it in WRF. The scheme uses the vertical integration of forecasted cloud water content to transform control variables to the observation space, and creates the correlations between Cw and control variables in the flow-dependent background error covariance based on all the ensemble members, so that the observed cloud information can affect the background temperature and humidity. For two typhoons in 2018 (Yagi and Rumiba), assimilating Cw significantly increases the simulated rainfalls and TC intensities. In terms of the average equitable threat score of daily moderate to heavy rainfall (5-120 mm), the improvements are over 130%, and the dry biases are cut by about 30%. Such improvements are traced down to the fact that Cw assimilation increases the moisture content, especially that further away from the TC center, which provides more precipitable water for the rainfall, strengthens the TC and broadens the TC size via latent heat release and internal wind field adjustment. [ABSTRACT FROM AUTHOR]

The possible paths of dimethyl ether (DME) synthesis from methanol over hydrated -Al2O3(110) in vacuum and liquid paraffin have been investigated by using density functional theory (DFT). Over hydrated -Al2O3(110), the three possible paths of methanol dehydration to DME have been investigated by the DFT method in vacuum and liquid paraffin. DME synthesis from methanol is carried out along the same pathway 2CH3OH(g) 2* 2CH3OH* 2CH3O* 2H* CH3OCH3* H2O* in vacuum and liquid paraffin, and the step of highest energy barrier is the reaction of 2CH3O* CH3OCH3* O*. The energy barrier of the step in liquid paraffin is higher than that in vacuum by 0.33eV. The surface acid strength in liquid paraffin decreases over -Al2O3(110) surface comparing with vacuum, showing that stronger surface acid strength benefits to DME synthesis. Our result is in consistent with the experiment results. DME synthesis from methanol is carried out along the same pathway 2CH3OH(g) + 2* → 2CH3OH* → 2CH3O* + 2H* → CH3OCH3* + H2O* in vacuum and liquid paraffin. The highest energy barrier is the reaction of 2CH3O* → CH3OCH3* + O*, and the energy barrier of the step in liquid paraffin is higher than that in vacuum by 0.33eV. The surface acid strength in liquid paraffin decreases over -Al2O3(110) surface comparing with vacuum, showing that stronger surface acid strength benefits to DME synthesis. [ABSTRACT FROM AUTHOR]

Noble metal nanoparticles are promising catalysts in electrochemical reactions, while understanding the relationship between the structure and reactivity of the particles is important to achieve higher efficiency of electrocatalysis, and promote the development of single-molecule electrochemistry. Electrogenerated chemiluminescence (ECL) was employed to image the catalytic oxidation of luminophore at single Au, Pt, and Au-Pt Janus nanoparticles. Compared to the monometal nanoparticles, the Janus particle structure exhibited enhanced ECL intensity and stability, indicating better catalytic efficiency. On the basis of the experimental results and digital simulation, it was concluded that a concentration difference arose at the asymmetric bimetallic interface according to different heterogeneous electron-transfer rate constants at Au and Pt. The fluid slip around the Janus particle enhanced local redox reactions and protected the particle surface from passivation., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Objective: To investigate the effect of the environmental carcinogenic factor-TCDD (2, 3, 7, 8-tetrachlorodibenzo-p-dioxin) on cell apoptosis and gene regulation of insulin-like growth factor binding protein 6 (IGFBP-6) in osteogenic sarcoma (SaOS-2) cells., Methods: The SaOS-2 cells were cultured with TCDD (1 x 10(-9), 1 x 10(-8), 1 x 10(-7) mol/L) for 24 hours. The MTT reduction assay and flow cytometry were used to measure the cell proliferation and the cell apoptosis in TCDD-treated SaOS-2 cells. The Nitrophenol phosphate salt method was used to measure activity of alkaline phosphatase (ALP) in SaOS-2 cells. The IGFBP-6 mRNA and protein in SaOS-2 cells were detected by reverse transcription polymerase chain reaction (RT-PCR) and western blotting analysis., Results: SaOS-2 cell proliferation was up-regulated with TCDD (1 x 10(-9), 1 x 10(-8), and 1 x 10(-7) mol/L) about 20%, 47% and 93% (18.4 +/- 4.5, 22.5 +/- 3.6 and 29.4 +/- 4.2), respectively. The synthesis of ALP was up-regulated about 28%, 95%, and 142% (1.12 +/- 0.28, 1.58 +/- 0.14 and 1.96 +/- 0.17), respectively (P < 0.05). The cell apoptosis was down-regulated in dose-dependent biological manner about 5%, 26% and 52%, respectively (P < 0.05). The expression of IGFBP-6 mRNA and protein was decreased in 1 x 10(-7) mol/L TCDD-treated SaOS-2 cells about 76% and 72% (P < 0.05)., Conclusion: TCDD at low concentration may have the negative effect on cell apoptosis and down-regulation on gene expression of IGFBP-6 in SaOS-2 cells.

Objective: To investigate the relationship between the HLA-DQB1 allele polymorphisms and the clinical features of 15 familial myasthenia gravis (MG) cases in north China., Methods: By polymerase chain reaction-sequence specific primers (PCR-SSP), the HLA-DQB1 gene polymorphisms were determined in 64 MG patients (15 familial and 49 sporadic) and 52 healthy individuals as control group. The clinical characteristics of 15 familial MG patients and 49 sporadic were analyzed. The measurement data was analyzed by t test and enumeration data by chi-square test., Results: The frequency of DQB1*0501 was significantly increased in familial MG, especially in the ocular type, compared with sporadic MG (P<0.05, OR=3.08) and healthy controls (P<0.01, OR=4.439). Comparing with healthy controls, the frequency of DQB1*0301/4 was increased (P<0.05, OR=2.56), while the frequency of DQB1*0601 was significantly decreased (P<0.05, OR=0.33) in sporadic MG. The familial patients had an early age of disease onset, but less severity and good prognosis., Conclusion: The familial MG has distinctive clinical features. DQB1*0501 allele is positively related to the genetic susceptibility to familial MG patients in north China, especially to the ocular type. DQB1*0301/4 allele is positively related to the pathogenesis of sporadic MG. DQB1*0601 may be a protecting allele for sporadic MG. The phenotype of MG may be the result of interaction of hereditary defects and environmental factors. The familial MG may be different from sporadic patients in genetic immune mechanism.