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The landfill leachate treated by sonication in presence of Fe2+ (US/Fe2+) and then by photo-Fenton achieved the highest total organic carbon (TOC) removal efficiency among the screened processes. The lower initial pH, dosage of Fe2+ and initial concentration of leachate were helpful in raising TOC removal efficiency of leachate by US/Fe2+. The optimal conditions for the US-photo-Fenton process were as follows: initial pH at 3.0, [H2O2]/[TOC0] at 2, [H2O2]/[Fe2+] at 5 and initial concentration of landfill leachate at 600 mg/L. The removal efficiency of TOC, chemical oxygen demand (COD) and 5-day biochemical oxygen demand (BOD5) were 68.3, 79.6 and 58.2%, while the BOD5/COD rose from 0.20 to 0.43 at optimum condition. Based on gas chromatography-mass spectrometry (GC-MS) results, 36 of a total of 56 pollutants were completely degraded by US-photo-Fenton treatment. [ABSTRACT FROM AUTHOR]

Somatic embryogenesis is an important model system for studies on embryo development. In order to gain a better understanding of the molecular mechanisms underlying somatic embryogenesis, RNA-Seq data were generated from non-embryogenic callus, embryogenic callus, somatic embryos, early-germinated somatic embryos, and lategerminated somatic embryos of Fragaria × ananassa Duch.'Benihopp'.Totals of 21 × 106 - 34 × 106 high-quality reads were obtained from the various RNA-seq libraries, with average lengths of 87 - 94 base pairs. A total of 81% of the reads were mapped to the reference Fragaria vesca genome and 946 differentially-expressed genes were identified, most of which were associated with non-embryonic callus. An analysis of the expression data using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database indicated that the gene ontology (GO) classes with the highest representation were 'metabolic process' and 'metabolism'. In total, 38 transcription factors were identified, including members of the AP2/EREBP and MYB families. Three genes related to hormone signalling pathways were identified: one gene encoding the AUXIN RESISTANT 1 (AUX1) protein and two genes encoding pathogenesisrelated 1 (PR1) proteins. During somatic embryogenesis, levels of transcripts corresponding to the AUX1 protein increased, while those corresponding to PR1 decreased. Non-embryogenic callus showed the greatest difference in transcriptome profile compared with the other samples, and the GO annotation term 'metabolism pathways' was particularly common in the data associated with strawberry somatic embryogenesis. Based on the RNA-seq data, we speculate that the phytohormones auxin and salicylic acid may play important roles during somatic embryogenesis. Our transcriptome dataset represents a valuable resource for researchers studying somatic embryogenesis and has the potential to provide new insights into the underlying mechanisms of molecular regulation. [ABSTRACT FROM AUTHOR]

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Through the effort in nearly 40 years of Tanzania, the total number of reported cases, the prevalence of HIV/AIDS and the number of death have declined, while the number of patients receiving antiretroviral therapy has increased significantly in Tanzania. At the same time, however, there are new challenges that require more attention, such as the HIV spread to rural areas, middle and lower social classes, and female teenagers. Although the overall performance of the HIV/AIDS prevention and control response is great, there is still a need to survey the new trends of this disease at the micro-level in Tanzania. In addition, there is a necessity of interdisciplinary concern due to the prevalence of heterosexual anal sex in Tanzania.

Objective: To study the clinicopathologic features of lymphoproliferative disease by lymph node core needle biopsy(CNB)and to evaluate the diagnostic significance of CNB for lymphoproliferative disease. Methods: The annual distribution, entity constitute, clinical finding, gross feature, morphologic change, affiliate study and repeat biopsy diagnosis of 1 013 cases of lymph node CNB diagnosed at West China Hospital of Sichuan University from January 2009 to December 2015 were investigated. Results: (1) Proportion of lymph node CNB in total amount of biopsy specimens increased from 0.2% in 2009 to 0.8% in 2015.(2) The study cohort included 471 lymphomas, 12 atypical lymphoid hyperplasia (ALH), 136 suspected lymphomas, 372 benign lesions, and 22 cases of descriptive diagnoses. The most common types were diffuse large B cell lymphoma and T-lymphoblastic lymphoma. (3) Majority of patients were adolescents and children younger than 20 years or the elderly older than 60 years. 53.1% CNB tumor specimen consisted of ≥4 tissue cores and 40.5% were >2 cm in length. (4) 104 CNB cases with previous history of excision biopsy was included 45 carcinomas(no metastatic carcinoma was found), 32 lymphomas for treatment observation.1/14 suspicious lymphomas, 1/1 ALH and 3/22 cases benign lesions were diagnosed as lymphoma by repeat biopsy respectively. (5) 217 CNB cases were diagnosed as lymphoma by subsequent CNB (70), or subsequent excision biopsy (147) including 78.5%(73/93) suspected lymphomas, 5/7 ALH and 32.3%(20/62)benign lesions. Conclusions: Lymph node CNB has certain clinical indications, although limited for the diagnosis of lymphoproliferative disorders. Suspected lymphomas and ALH diagnosed by CNB should be followed by repeat tissue biopsy. For the benign lesions by CNB it does not rule out additional biopsy to further investigate the lesion.

Objective: To explore the efficacy and safety of tertiary hospital guided and community-driven family self-help cardiac rehabilitation model. Methods: This study was a prospective randomized controlled study, 80 patients from Beijing Electrical Power Hospital and Beijing Jingmei Group General Hospital with acute coronary syndrome were included from June to December 2015 and divided into 2 groups. Patients in rehabilitation group ( n =52) received tertiary hospital(Peiking University Peoples' Hospital) guided and community-driven family self-help cardiac rehabilitation for 3 months, and patients in control group ( n =28) received routine secondary treatment for 3 months. Following parameters including 6 minutes walk distance, score of life quality (evaluated by Short Form-12), score of anxiety (evaluated by Generalized Anxiety Disorder-7), score of depression (evaluated by Perceived Health Questionnaire-9), self-management competency (evaluated by questionnaire) were collected at baseline and after treatment for 3 months. Results: Compared with control group, 6 minutes walk distance was longer in rehabilitation group((60.2±6.8) meters vs. (24.9±10.5)meters, P <0.01). The difference values between after and before intervention of life quality scores((0.14±3.90)scores vs.(-7.44±5.85)scores, P >0.05), anxiety scores((-0.16±2.12 ) scores vs.(0.70±1.13)scores, P >0.05) and depression scores((-1.17±2.79) scores vs.(0.60±0.36)scores, P >0.05) were similar between the 2 groups. The amplification of patients with regular exercise (50.26% vs. 0, P <0.05), limit sugary foods usually and always (53.22% vs. 3.98%, P <0.05), eat 200-400 g fruits usually and always (78.61 % vs. 0, P <0.05), eat 300-500 g vegetables usually and always (9.74% vs. 0, P <0.05), and answering very confident to questions such as let the physicians know about your diseases (40.17% vs. 5.00%, P <0.05), know how to take medicines (44.52% vs. 5.00%, P <0.05), know how much exercise was right for yourself (26.43% vs.0, P <0.05) were significantly higher in rehabilitation group than in control group. There were no cardiac rehabilitation training related cardiovascular events. Conclusion: Tertiary hospital guided and community-driven family self-help cardiac rehabilitation model is an effective and safe management model of cardiovascular disease in chronic phase, and it is necessary to further expand the study population to verify the efficacy of this model.

Objective: To test the Beijing questionnaire as a means of identifying patients with obstructive sleep apnea hypopnea syndrome（OSAHS）. Method: The Beijing questionnaire is designed as an explorative tool consist of 11 questions for patients with obstructive sleep apnea hypopnea, and is targeted toward key symptoms include snoring, apneas, daytime sleepiness, hypertension and overweight. 1 336 female participants living in communities of age≥40 years and 198 male adult subjects visting clinics were given questionnaires. Finally, 59 female and 198 male subjects underwent sleep studies after factor analysis,reliability check,internal consistency study. The correlation analysis was performed between the scores from the Beijing questionnaire and the apnea-hypopnea index from inlaboratory polysomnography.Receiver operating characteristics were constructed to determine optimal sensitivity and specificity. Twenty-four male subjects were recorded in the sleep laberatory again after operative. Result: Factor analysis reduced 11 questions of scale to four common factors as we have designed: snoring,apneas,other symptoms,risk factors. Cronbach's α coefficient of scale reached 0.7.There were an acceptable level of testretest reliability(r=0.619, P <0.01).The apnea hypopnea indices were significantly correlated with their Beijing questionnaire scores( P <0.01).For wemen,an Beijing questionnaire scroe of 19.5 provided a sensitivity of 74.3% and a specificity of 62.5%.For men,an Beijing questionnaire scroe of 22.5 provided a sensitivity of 90.9% and a specificity of 54.5%. And the postoperative Beijing questionnaire scroes changed with the apnea hypopnea indices. Conclusion: This questionnaire has a good validity and reliability and appears to be valid and sensitive to clinical change., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

The landfill leachate treated by sonication in presence of Fe(2+) (US/Fe(2+)) and then by photo-Fenton achieved the highest total organic carbon (TOC) removal efficiency among the screened processes. The lower initial pH, dosage of Fe(2+) and initial concentration of leachate were helpful in raising TOC removal efficiency of leachate by US/Fe(2+). The optimal conditions for the US-photo-Fenton process were as follows: initial pH at 3.0, [H2O2]/[TOC0] at 2, [H2O2]/[Fe(2+)] at 5 and initial concentration of landfill leachate at 600 mg/L. The removal efficiency of TOC, chemical oxygen demand (COD) and 5-day biochemical oxygen demand (BOD5) were 68.3, 79.6 and 58.2%, while the BOD5/COD rose from 0.20 to 0.43 at optimum condition. Based on gas chromatography-mass spectrometry (GC-MS) results, 36 of a total of 56 pollutants were completely degraded by US-photo-Fenton treatment.

Two epimeric pairs of iridoid aglycones, named shanzhigenin methyl ester and 1-epishanzhigenin methyl ester, and 8-acetylshanzhigenin methyl ester and 8-acetyl-1-epishanzhigenin methyl ester, were isolated from Phlomis umbrosa roots, along with five known iridoid glucosides. The four iridoid aglycones are reported for the first time from a natural source. Their structures were established by spectroscopic methods, mainly 1D- and 2D-NMR spectroscopic experiments, and chemical methods.

With the development of Micro Electro Mechanical System(MEMS), it's application in the fields of biomedical engineering becomes a vital consideration. This paper describes the advantages of Minimally invasive Surgery (MIS) and its applications. A novel medical endoscope driven by micro robot is also introduced.

Pulsed field gel electrophoresis was used to construct a long-range map of the normal BCR gene. A single BssHII restriction fragment encompasses all the known exons of the BCR gene (except a small 5' part of exon one). MIuI has one restriction site within the first intron of the BCR gene and another 250 kb downstream. This MIuI fragment contains most of the BCR gene coding sequences apart from the first exon and contains more sequences downstream of the BCR gene than the BssHII fragment. The NarI restriction sites are very close to the BssHII sites in the BCR gene, but they differ in the ABL gene, so that NarI digests could theoretically provide additional information in chronic myeloid leukaemia (CML) patients. This map was used to confirm BCR gene involvement in two CML patients in whom results of conventional Southern blotting of DNA were ambiguous. It was also used in a third patient to demonstrate the presence of a breakpoint apparently outside the BCR gene. Preliminary evidence from the use of PFGE confirms the presence of three BCR-related genes homologous to 3' sequences in the classical BCR gene (BCR-1). These BCR-related genes are located at a considerable distance from BCR-1.

Forty-eight patients treated by allogeneic bone marrow transplantation (BMT) for Philadelphia (Ph) chromosome-positive chronic myeloid leukemia in chronic phase had serial cytogenetic studies of marrow performed at intervals after transplant. Twenty patients received marrow cells from donors of opposite sex. Ph+ marrow metaphases were identified in 24 of 48 (50%) of patients after BMT; they were first seen early (within 1 year) in 16 cases and late (greater than 1 year after BMT) in eight cases. Ph-positivity after BMT occurred more commonly in recipients of T-depleted than nondepleted marrow (19 of 28 v 5 of 20). In 4 cases the Ph+ metaphases were found only transiently after BMT; in 11 cases the Ph+ metaphases have persisted but hematologic relapse has not ensued; in 9 cases the finding of Ph+ metaphases coincided with or preceded hematologic relapse. Chromosomes in cells of donor origin had morphological abnormalities in two cases. No relapses were identified in cells of donor origin. Our data suggest that the relationship between cells of recipient and donor origin is complex: cure of leukemia may depend on factors that operate for some months or years after BMT.