Search

Your search keyword '"Gantet, Pascal"' showing total 461 results
Start Over Author "Gantet, Pascal"
461 results on '"Gantet, Pascal"'

4. Quantitative trait loci for grain mineral element accumulation in Vietnamese rice landraces.

Author

Tran, Hien Linh, Hoang, Giang Thi, Phung, Nhung Thi Phuong, Le, Ham Huy, Grondin, Alexandre, and Gantet, Pascal

Subjects
LOCUS (Genetics), BROWN rice, ION transport (Biology), HEAVY metals, RICE, BIOFORTIFICATION, ARSENIC
Abstract

Rice (Oryza sativa L.) is a staple food for half of the world's population, and its biofortification is a key factor in fighting micronutrient malnutrition. However, harmful heavy metals tend to accumulate in rice grains due to soil and water contamination. Therefore, it is important to improve beneficial micronutrient contents and reduce the accumulation of undesirable metals in rice grain. To better characterize the genetic control of mineral accumulation in rice, we conducted association genetics on the ion contents of white and brown grains using a collection of 184 Vietnamese rice landraces. In total, 27 significant associations were identified and delimited into quantitative trait loci associated with macronutrients such as phosphorus, potassium or calcium; micronutrients such as iron or zinc; or toxic heavy metals such as arsenic and cadmium. Several genes related to ion homeostasis or ion transport were identified in the different quantitative trait loci. LOC_Os10g30610, present in qRAs10-1 associated with arsenic content in brown rice, encodes an ABC transporter (OsABCG25), which is involved in the silicon-induced formation of the Casparian strip in the rice exodermis and could act as a barrier restricting As diffusion within the root cortex. LOC_Os05g04330, present in qRP5-1 and associated with phosphorus content in brown rice, encodes a CHH methylation maintenance protein, and its expression is downregulated in roots in the presence of the phosphorus uptake 1 (Pup1), suggesting a role for epigenetics in the regulation of phosphorus uptake and accumulation in grain. These findings reveal novel quantitative trait loci associated with grain ion content and candidate genes that are potentially valuable for breeding programs aimed at rice grain biofortification and reducing toxic metal accumulation. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

5. Nitrate and ammonium, the yin and yang of nitrogen uptake: a time-course transcriptomic study in rice.

Author

Pélissier, Pierre-Mathieu, Parizot, Boris, Jia, Letian, De Knijf, Alexa, Goossens, Vera, Gantet, Pascal, Champion, Antony, Audenaert, Dominique, Wei Xuan, Beeckman, Tom, and Motte, Hans

Subjects
AMMONIUM nitrate, CROP yields, ESSENTIAL nutrients, CROP growth, PLANT nutrients
Abstract

Nitrogen is an essential nutrient for plants and a major determinant of plant growth and crop yield. Plants acquire nitrogen mainly in the form of nitrate and ammonium. Both nitrogen sources affect plant responses and signaling pathways in a different way, but these signaling pathways interact, complicating the study of nitrogen responses. Extensive transcriptome analyses and the construction of gene regulatory networks, mainly in response to nitrate, have significantly advanced our understanding of nitrogen signaling and responses in model plants and crops. In this study, we aimed to generate a more comprehensive gene regulatory network for the major crop, rice, by incorporating the interactions between ammonium and nitrate. To achieve this, we assessed transcriptome changes in rice roots and shoots over an extensive time course under single or combined applications of the two nitrogen sources. This dataset enabled us to construct a holistic co-expression network and identify potential key regulators of nitrogen responses. Next to known transcription factors, we identified multiple new candidates, including the transcription factors OsRLI and OsEIL1, which we demonstrated to induce the primary nitrate-responsive genes OsNRT1.1b and OsNIR1. Our network thus serves as a valuable resource to obtain novel insights in nitrogen signaling. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

Author

de la Fuente, Carla, primary, Grondin, Alexandre, primary, Sine, Bassirou, additional, Debieu, Marilyne, additional, Belin, Christophe, additional, Hajjarpoor, Amir, additional, Atkinson, Jonathan A, additional, Passot, Sixtine, additional, Salson, Marine, additional, Orjuela, Julie, additional, Tranchant-Dubreuil, Christine, additional, Brossier, Jean-Rémy, additional, Steffen, Maxime, additional, Morgado, Charlotte, additional, Dinh, Hang Ngan, additional, Pandey, Bipin K, additional, Darmau, Julie, additional, Champion, Antony, additional, Petitot, Anne-Sophie, additional, Barrachina, Celia, additional, Pratlong, Marine, additional, Mounier, Thibault, additional, Nakombo-Gbassault, Princia, additional, Gantet, Pascal, additional, Gangashetty, Prakash, additional, Guedon, Yann, additional, Vadez, Vincent, additional, Reichheld, Jean-Philippe, additional, Bennett, Malcolm J, additional, Kane, Ndjido Ardo, additional, Guyomarc'h, Soazig, additional, Wells, Darren M, additional, Vigouroux, Yves, additional, and Laplaze, Laurent, additional

Published
2024
Full Text
View/download PDF

9. Author Response: Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

Author

de la Fuente Canto, Carla, primary, Grondin, Alexandre, additional, Sine, Bassirou, additional, Debieu, Marilyne, additional, Belin, Christophe, additional, Hajjarpoor, Amir, additional, Atkinson, Jonathan, additional, Passot, Sixtine, additional, Salson, Marine, additional, Orjuela, Julie, additional, Tranchant, Christine, additional, Brossier, Jean-Rémy, additional, Steffen, Maxime, additional, Morgado, Charlotte, additional, Dinh, Hang Ngan, additional, Pandey, Bipin Kumar, additional, Darmau, Julie, additional, Champion, Antony, additional, Petitot, Anne-Sophie, additional, Barrachina, Celia, additional, Pratlong, Marine, additional, Mounier, Thibault, additional, Nakombo-Gbassault, Princia, additional, Gantet, Pascal, additional, Gangashetty, Prakash, additional, Guedon, Yann, additional, Vadez, Vincent, additional, Reichheld, Jean-Philippe, additional, Bennett, Malcolm J, additional, Kane, Ndjido Ardo, additional, Guyomarc'h, Soazig, additional, Wells, Darren M, additional, Vigouroux, Yves, additional, and Laplaze, Laurent, additional

Published
2024
Full Text
View/download PDF

10. Author Response: Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

Author

de la Fuente, Carla, primary, Grondin, Alexandre, additional, Sine, Bassirou, additional, Debieu, Marilyne, additional, Belin, Christophe, additional, Hajjarpoor, Amir, additional, Atkinson, Jonathan A., additional, Passot, Sixtine, additional, Salson, Marine, additional, Orjuela, Julie, additional, Tranchant-Dubreuil, Christine, additional, Brossier, Jean-Rémy, additional, Steffen, Maxime, additional, Morgado, Charlotte, additional, Dinh, Hang Ngan, additional, Pandey, Bipin K., additional, Darmau, Julie, additional, Champion, Antony, additional, Petitot, Anne-Sophie, additional, Barrachina, Celia, additional, Pratlong, Marine, additional, Mounier, Thibault, additional, Nakombo-Gbassault, Princia, additional, Gantet, Pascal, additional, Gangashetty, Prakash, additional, Guédon, Yann, additional, Vadez, Vincent, additional, Reichheld, Jean-Philippe, additional, Bennett, Malcolm J., additional, Kane, Ndjido, additional, Guyomarc’h, Soazig, additional, Wells, Darren M., additional, Vigouroux, Yves, additional, and Laplaze, Laurent, additional

Published
2024
Full Text
View/download PDF

11. Transcriptional changes during crown-root development and emergence in barley (Hordeum vulgare L.).

Author

Dieu Thu Nguyen, Kokáš, Filip Zavadil, Gonin, Mathieu, Lavarenne, Jérémy, Colin, Myriam, Gantet, Pascal, and Bergougnoux, Véronique

Abstract

Background Roots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering. Results Here, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identifed 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modifcation, secondary metabolism. Our results highlighted genes that are likely involved in the diferent steps of crown root formation from initiation to primordia diferentiation and emergence, and revealed the activation of diferent hormonal pathways during this process. Conclusions This whole transcriptomic study is the frst study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modifcation, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet

Author

de la Fuente, Carla, primary, Grondin, Alexandre, additional, Sine, Bassirou, additional, Debieu, Marilyne, additional, Belin, Christophe, additional, Hajjarpoor, Amir, additional, Atkinson, Jonathan A., additional, Passot, Sixtine, additional, Salson, Marine, additional, Orjuela, Julie, additional, Tranchant-Dubreuil, Christine, additional, Brossier, Jean-Rémy, additional, Steffen, Maxime, additional, Morgado, Charlotte, additional, Dinh, Hang Ngan, additional, Pandey, Bipin K., additional, Darmau, Julie, additional, Champion, Antony, additional, Petitot, Anne- Sophie, additional, Barrachina, Celia, additional, Pratlong, Marine, additional, Mounier, Thibault, additional, Gantet, Pascal, additional, Gangashetty, Prakash, additional, Guédon, Yann, additional, Vadez, Vincent, additional, Reichheld, Jean-Philippe, additional, Bennett, Malcolm J., additional, Kane, Ndjido, additional, Guyomarc’h, Soazig, additional, Wells, Darren M., additional, Vigouroux, Yves, additional, and Laplaze, Laurent, additional

Published
2023
Full Text
View/download PDF

17. Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

Author

de la Fuente, Carla, Grondin, Alexandre, Sine, Bassirou, Debieu, Maryline, Belin, Christophe, Hajjarpoor, Amir, Atkinson, Jonathan, Passot, Sixtine, Salson, Marine, Orjuela, Julie, Tranchant-Dubreuil, Christine, Brossier, Jean-Rémy, Steffen, Maxime, Morgado, Charlotte, Dinh, Hang Ngan, Pandey, Bipin K., Darmau, Julie, Champion, Anthony, Petitot, Anne-Sophie, Barrachina, Célia, Pratlong, Marine, Mounier, Thibault, Nakombo-Gbassault, Princia, Gantet, Pascal, Gangashetty, Prakash, Guédon, Yann, Vadez, Vincent, Reichheld, Jean-Philippe, Bennett, Malcolm J., Kane Ndjido, Ardo, Guyomarc'h, Soazig, Wells, Darren, Vigouroux, Yves, Laplaze, Laurent, de la Fuente, Carla, Grondin, Alexandre, Sine, Bassirou, Debieu, Maryline, Belin, Christophe, Hajjarpoor, Amir, Atkinson, Jonathan, Passot, Sixtine, Salson, Marine, Orjuela, Julie, Tranchant-Dubreuil, Christine, Brossier, Jean-Rémy, Steffen, Maxime, Morgado, Charlotte, Dinh, Hang Ngan, Pandey, Bipin K., Darmau, Julie, Champion, Anthony, Petitot, Anne-Sophie, Barrachina, Célia, Pratlong, Marine, Mounier, Thibault, Nakombo-Gbassault, Princia, Gantet, Pascal, Gangashetty, Prakash, Guédon, Yann, Vadez, Vincent, Reichheld, Jean-Philippe, Bennett, Malcolm J., Kane Ndjido, Ardo, Guyomarc'h, Soazig, Wells, Darren, Vigouroux, Yves, and Laplaze, Laurent

Abstract

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet's early root system features a single fast-growing primary root which we hypothesize is an adaptation to the Sahelian climate. Using crop modeling, we demonstrate that early drought stress is an important constraint in agrosystems in the Sahel where pearl millet was domesticated. Furthermore, we show that increased pearl millet primary root growth is correlated with increased early water stress tolerance in field conditions. Genetics including genome-wide association study and quantitative trait loci (QTL) approaches identify genomic regions controlling this key root trait. Combining gene expression data, re-sequencing and re-annotation of one of these genomic regions identified a glutaredoxin-encoding gene PgGRXC9 as the candidate stress resilience root growth regulator. Functional characterization of its closest Arabidopsis homolog AtROXY19 revealed a novel role for this glutaredoxin (GRX) gene clade in regulating cell elongation. In summary, our study suggests a conserved function for GRX genes in conferring root cell elongation and enhancing resilience of pearl millet to its Sahelian environment.

Published
2023

18. Genome-wide association study in two-row spring barley landraces identifies QTL associated with plantlets root system architecture traits in well-watered and osmotic stress conditions

Author

Khodaeiaminjan, Mortaza, primary, Knoch, Dominic, additional, Ndella Thiaw, Marie Rose, additional, Marchetti, Cintia F., additional, Kořínková, Nikola, additional, Techer, Alexie, additional, Nguyen, Thu D., additional, Chu, Jianting, additional, Bertholomey, Valentin, additional, Doridant, Ingrid, additional, Gantet, Pascal, additional, Graner, Andreas, additional, Neumann, Kerstin, additional, and Bergougnoux, Véronique, additional

Published
2023
Full Text
View/download PDF

20. OsMADS26 Negatively Regulates Resistance to Pathogens and Drought Tolerance in Rice

Author

Khong, Giang Ngan, Pati, Pratap Kumar, Richaud, Frédérique, Parizot, Boris, Bidzinski, Przemyslaw, Mai, Chung Duc, Bès, Martine, Bourrié, Isabelle, Meynard, Donaldo, Beeckman, Tom, Selvaraj, Michael Gomez, Manabu, Ishitani, Genga, Anna-Maria, Brugidou, Christophe, Do, Vinh Nang, Guiderdoni, Emmanuel, Morel, Jean-Benoit, and Gantet, Pascal

Published
2015

26. PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana

Author

Bellande, Kevin, Trinh, Duy-Chi, Gonzalez, Anne-Alicia, Dubois, Emeric, Petitot, Anne-Sophie, Lucas, Mikaël, Champion, Antony, Gantet, Pascal, Laplaze, Laurent, Guyomarc’h, Soazig, Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Université de Montpellier (UM), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-BioCampus (BCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ROSSI, Sabine, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and ANR-17-CE13-0004,NewRoot,Propriétés du réseau de gènes contrôlant la formation des nouvelles racines latérales chez les plantes(2017)

Subjects
[SDE] Environmental Sciences, patterning, PLETHORA, Indoleacetic Acids, Arabidopsis Proteins, organogenesis, Arabidopsis, PUCHI, Plant Roots, meristem, lateral root development, cytokinin, Gene Expression Regulation, Plant, [SDE]Environmental Sciences, auxin, Transcription Factors
Abstract

International audience; Lateral root organogenesis is a key process in the development of a plant’s root system and its adaptation to the environment. During lateral root formation, an early phase of cell proliferation first produces a four-cell-layered primordium, and only from this stage onwards is a root meristem-like structure, expressing root stem cell niche marker genes, being established in the developing organ. Previous studies reported that the gene regulatory network controlling lateral root formation is organized into two subnetworks whose mutual inhibition may contribute to organ patterning. PUCHI encodes an AP2/ERF transcription factor expressed early during lateral root primordium development and required for correct lateral root formation. To dissect the molecular events occurring during this early phase, we generated time-series transcriptomic datasets profiling lateral root development in puchi-1 mutants and wild types. Transcriptomic and reporter analyses revealed that meristem-related genes were expressed ectopically at early stages of lateral root formation in puchi-1 mutants. We conclude that, consistent with the inhibition of genetic modules contributing to lateral root development, PUCHI represses ectopic establishment of meristematic cell identities at early stages of organ development. These findings shed light on gene network properties that orchestrate correct timing and patterning during lateral root formation.

Published
2022
Full Text
View/download PDF

29. CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice

Author

Gonin, Mathieu, primary, Jeong, Kwanho, additional, Coudert, Yoan, additional, Lavarenne, Jeremy, additional, Hoang, Giang Thi, additional, Bes, Martine, additional, To, Huong Thi Mai, additional, Thiaw, Marie‐Rose Ndella, additional, Do, Toan Van, additional, Moukouanga, Daniel, additional, Guyomarc'h, Soazig, additional, Bellande, Kevin, additional, Brossier, Jean‐Rémy, additional, Parizot, Boris, additional, Nguyen, Hieu Trang, additional, Beeckman, Tom, additional, Bergougnoux, Véronique, additional, Rouster, Jacques, additional, Sallaud, Christophe, additional, Laplaze, Laurent, additional, Champion, Antony, additional, and Gantet, Pascal, additional

Published
2022
Full Text
View/download PDF

30. CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice

Author

Gonin, Mathieu, Jeong, Kwanho, Coudert, Yoan, Lavarenne, Jeremy, Hoang, Giang Thi, Bes, Martine, To, Huong Thi Mai, Thiaw, Marie-Rose Ndella, Do, Toan Van, Moukouanga, Daniel, Guyomarc'h, Soazig, Bellande, Kevin, Brossier, Jean-Rémy, Parizot, Boris, Nguyen, Hieu Trang, Beeckman, Tom, Bergougnoux, Véronique, Rouster, Jacques, Sallaud, Christophe, Laplaze, Laurent, Champion, Anthony, Gantet, Pascal, Gonin, Mathieu, Jeong, Kwanho, Coudert, Yoan, Lavarenne, Jeremy, Hoang, Giang Thi, Bes, Martine, To, Huong Thi Mai, Thiaw, Marie-Rose Ndella, Do, Toan Van, Moukouanga, Daniel, Guyomarc'h, Soazig, Bellande, Kevin, Brossier, Jean-Rémy, Parizot, Boris, Nguyen, Hieu Trang, Beeckman, Tom, Bergougnoux, Véronique, Rouster, Jacques, Sallaud, Christophe, Laplaze, Laurent, Champion, Anthony, and Gantet, Pascal

Abstract

In cereals, the root system is mainly composed of post-embryonic shoot-borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to the ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, is a key regulator of crown root initiation in rice (Oryza sativa). Here, we show that CRL1 can bind, both in vitro and in vivo, not only the LBD-box, a DNA sequence recognized by several ASL/LBD transcription factors, but also another not previously identified DNA motif that was named CRL1-box. Using rice protoplast transient transactivation assays and a set of previously identified CRL1-regulated genes, we confirm that CRL1 transactivates these genes if they possess at least a CRL1-box or an LBD-box in their promoters. In planta, ChIP-qPCR experiments targeting two of these genes that include both a CRL1- and an LBD-box in their promoter show that CRL1 binds preferentially to the LBD-box in these promoter contexts. CRISPR/Cas9-targeted mutation of these two CRL1-regulated genes, which encode a plant Rho GTPase (OsROP) and a basic helix–loop–helix transcription factor (OsbHLH044), show that both promote crown root development. Finally, we show that OsbHLH044 represses a regulatory module, uncovering how CRL1 regulates specific processes during crown root formation.

Published
2022

31. Physiological and genetic control of transpiration efficiency in African rice, Oryza glaberrima Steud

Author

Affortit, Pablo, primary, Effa-Effa, Branly, additional, Ndoye, Mame Sokhatil, additional, Moukouanga, Daniel, additional, Luchaire, Nathalie, additional, Cabrera-Bosquet, Llorenç, additional, Perálvarez, Maricarmen, additional, Pilloni, Raphaël, additional, Welcker, Claude, additional, Champion, Antony, additional, Gantet, Pascal, additional, Diedhiou, Abdala Gamby, additional, Manneh, Baboucarr, additional, Aroca, Ricardo, additional, Vadez, Vincent, additional, Laplaze, Laurent, additional, Cubry, Philippe, additional, and Grondin, Alexandre, additional

Published
2022
Full Text
View/download PDF

32. Exemples d'études de CropOmics : sur la diversité génomique (cours), sur des introgressions parmi 'les 3000 riz' (miniprojets)

Author

Bocs, Stéphanie, Gantet, Pascal, and Glaszmann, Jean-Christophe

Abstract

Au regard des changements globaux en cours, la sécurité alimentaire et nutritionnelle sont des préoccupations majeures. AGAP Institut s'appuie sur des grands projets de (re)séquençages de génomes pour comprendre les forces qui modèlent la diversité de plantes cultivées tropicales et méditerranéenne afin de mettre en oeuvre des programmes de sélection adaptés. A l'instar d'autres unités, il s'appuie sur la plateforme South Green multi-instituts de l'IFB qui offre un cadre à une science reproductible et des données FAIR. Ainsi, le projet Genome Harvest a mobilisé les biomathématiques, la bioinformatique, la génomique et la génétique afin de décrypter l'organisation et la dynamique des génomes de plantes cultivées majeures. Le projet Cultivar a de son côté permis de renforcer les liens entre recherche et enseignement autour de l'amélioration des plantes. La nouvelle maquette pédagogique de l'Université de Montpellier (LMD5, sept 2022) inclut l'UE Big Omics et Génomique Comparative du M2 du Master Biologie-Agrosciences de l'UM et l'Institut Agro de Montpellier. Dans ce module sont présentés des travaux de génomique évolutive de populations de trois espèces, bananier, canne à sucre et riz. Les deux premiers sont des modèles complexes (polyploïdes, interspécifiques, riches en anomalies cytogénétiques comme des translocations ou de l'aneuploïdie) alors que le riz a un génome réputé simple servant de modèle pour les monocotylédones. Nous éclairons la contribution de génomes de groupes génétiques fondateurs (parentaux, ancestraux) aux génomes modernes de cultivars. Nous proposons aux étudiants, sous la forme de mini-projets de recherche, de conduire des analyses plus pointues chez le riz en caractérisant des introgressions particulières à la recherche de gènes soumis à sélection et de variants à haute valeur adaptative potentielle. Il s'agit de petites régions génomiques échangées entre groupes variétaux (approche 1) ou de très courts segments à forte différentiation (approche 2). Certains exemples décryptés par les étudiants, seront illustrés. Ce front de science, dans l'environnement de Muse, participe à la construction d'un socle de connaissances sur l'identification de gènes candidats impliqués dans l'adaptation génétique des plantes, à l'interface entre génomique végétale, bioinformatique, production agricole et gestion de la biodiversité.

Published
2022

34. The roots of future rice harvests

Author

Ahmadi, Nourollah, Audebert, Alain, Bennett, Malcolm J, Bishopp, Anthony, de Oliveira, Antonio Costa, Courtois, Brigitte, Diedhiou, Abdala, Diévart, Anne, Gantet, Pascal, Ghesquière, Alain, Guiderdoni, Emmanuel, Henry, Amelia, Inukai, Yoshiaki, Kochian, Leon, Laplaze, Laurent, Lucas, Mikael, Luu, Doan Trung, Manneh, Baboucarr, Mo, Xiaorong, Muthurajan, Raveendran, Périn, Christophe, Price, Adam, Robin, Sabariappan, Sentenac, Hervé, Sine, Bassirou, Uga, Yusaku, Véry, Anne Aliénor, Wissuwa, Matthias, Wu, Ping, and Xu, Jian

Published
2014
Full Text
View/download PDF

40. High-throughput phenotyping reveals a link between transpiration efficiency and transpiration restriction under high evaporative demand and new loci controlling water use-related traits in African rice, Oryza glaberrima Steud.

Author

Affortit, Pablo, primary, Effa Effa, Branly, additional, Ndoye, Mame Sokhatil, additional, Moukouanga, Daniel, additional, Luchaire, Nathalie, additional, Cabrera-Bosquet, Llorenc, additional, Peralvarez, Marie Carmen, additional, Pilloni, Raphael, additional, Welcker, Claude, additional, Champion, Antony, additional, Gantet, Pascal, additional, Diedhiou, Abdala Gamby, additional, Manneh, Baboucarr, additional, Aroca, Ricardo, additional, Vadez, Vincent, additional, Laplaze, Laurent, additional, Cubry, Philippe, additional, and Grondin, Alexandre, additional

Published
2021
Full Text
View/download PDF

41. Morphological Analysis, Protein Profiling and Expression Analysis of Auxin Homeostasis Genes of Roots of Two Contrasting Cultivars of Rice Provide Inputs on Mechanisms Involved in Rice Adaptation towards Salinity Stress

Author

Saini, Shivani, Kaur, Navdeep, Marothia, Deeksha, Singh, Baldev, Singh, Varinder, Gantet, Pascal, and Pati, Pratap Kumar

Subjects
PIN, abiotic stress, rice, YUCCA, Botany, food and beverages, root, Article, salinity, proteomics, QK1-989, auxin, mass spectrometry
Abstract

Plants remodel their root architecture in response to a salinity stress stimulus. This process is regulated by an array of factors including phytohormones, particularly auxin. In the present study, in order to better understand the mechanisms involved in salinity stress adaptation in rice, we compared two contrasting rice cultivars-Luna Suvarna, a salt tolerant, and IR64, a salt sensitive cultivar. Phenotypic investigations suggested that Luna Suvarna in comparison with IR64 presented stress adaptive root traits which correlated with a higher accumulation of auxin in its roots. The expression level investigation of auxin signaling pathway genes revealed an increase in several auxin homeostasis genes transcript levels in Luna Suvarna compared with IR64 under salinity stress. Furthermore, protein profiling showed 18 proteins that were differentially regulated between the roots of two cultivars, and some of them were salinity stress responsive proteins found exclusively in the proteome of Luna Suvarna roots, revealing the critical role of these proteins in imparting salinity stress tolerance. This included proteins related to the salt overly sensitive pathway, root growth, the reactive oxygen species scavenging system, and abscisic acid activation. Taken together, our results highlight that Luna Suvarna involves a combination of morphological and molecular traits of the root system that could prime the plant to better tolerate salinity stress.

Published
2021

45. Genome-Wide Association Mapping of Salinity Tolerance at the Seedling Stage in a Panel of Vietnamese Landraces Reveals New Valuable QTLs for Salinity Stress Tolerance Breeding in Rice

Author

Le, Thao Duc, primary, Gathignol, Floran, additional, Vu, Huong Thi, additional, Nguyen, Khanh Le, additional, Tran, Linh Hien, additional, Vu, Hien Thi Thu, additional, Dinh, Tu Xuan, additional, Lazennec, Françoise, additional, Pham, Xuan Hoi, additional, Véry, Anne-Aliénor, additional, Gantet, Pascal, additional, and Hoang, Giang Thi, additional

Published
2021
Full Text
View/download PDF

47. Overexpression of MADS-box Gene AGAMOUS-LIKE 12 Activates Root Development in Juglans sp. and Arabidopsis thaliana

Author

Montiel, Grégory, Gaudet, Muriel, Laurans, Françoise, Rozenberg, Philippe, Simon, Matthieu, Gantet, Pascal, Jay-Allemand, Christian, Breton, Christian, Biologie intégrée pour la valorisation de la diversité des Arbres et de la Forêt (BioForA), Office National des Forêts (ONF)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de biologie et pathologie végétales (LBPV), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN), Research Institute on Terrestrial Ecosystems [CNR, Italy] (IRET), Consiglio Nazionale delle Ricerche (CNR), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Diversité, adaptation, développement des plantes (UMR DIADE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Office national des forêts (ONF)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
cell division, cell differentiation, root meristem, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, transcription factor, transgenic plant
Abstract

Until recently, the roles of plant MADS-box genes have mainly been characterized during inflorescence and flower differentiation. In order to precise the roles of AGAMOUS-LIKE 12, one of the few MADS-box genes preferentially expressed in roots, we placed its cDNA under the control of the double 35S CaMV promoter to produce transgenic walnut tree and Arabidopsis plants. In Juglans sp., transgenic somatic embryos showed significantly higher germination rates but abnormal development of their shoot apex prevented their conversion into plants. In addition, a wide range of developmental abnormalities corresponding to ectopic root-like structures affected the transgenic lines suggesting partial reorientations of the embryonic program toward root differentiation. In Arabidopsis, AtAGL12 overexpression lead to the production of faster growing plants presenting dramatically wider and shorter root phenotypes linked to increased meristematic cell numbers within the root apex. In the upper part of the roots, abnormal cell divisions patterns within the pericycle layer generated large ectopic cell masses that did not prevent plants to grow. Taken together, our results confirm in both species that AGL12 positively regulates root meristem cell division and promotes overall root vascular tissue formation. Genetic engineering of AGL12 expression levels could be useful to modulate root architecture and development.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results