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Thermal damage in heated bovine myocardial tissue is assessed from measured changes in total reflection and transmission of light. Mathematical expressions, based on random walk analysis of light propagation within tissue slabs, are used to relate the diffuse reflection and transmittance to the absorption coefficient, [[micron].sub.a], and effective scattering coefficient, [[micron][prime].sub.s] for samples of myocardial tissue which were subjected to rapid step changes in temperature. Time-dependent changes in [[micron][prime].sub.s] indicate two processes, one with a fast and temperature-dependent rate the other with a slow and apparently temperature-independent rate. For final temperatures above 56.8 [degrees] C and for the first 500 s after the temperature change, the optical parameters are well fit by exponential forms that exhibit temperature-dependent time constants as predicted by Arrhenius reaction rate theory of thermal damage. The scattering changes are associated with an apparent activation energy, [Delta]E, of 162 kJ/mole and a frequency constant, A, of 3x [10.sup.23] [s.sup.-1]. This method provides a means for estimating optical coefficients which are needed to assess laser tissue dosimetry.

We calculate the time-resolved flux of photons transmitted across an optically turbid slab containing a partially absorbing inclusion. An analytical expression is obtained for the flux at a detector positioned opposite a point source (at a distance equal to the thickness of the slab) when the center of the inclusion lies on the line connecting those points. The calculation employs a discrete-time lattice random-walk model of photon transport. The resulting expression is used to assess the affects of time resolution on the detectability of the inclusion. Key words: Random walks, optical imaging, detectability, optical contrast.

Monte Carlo simulations are used to discern scaling relationships for photon migration occurring within homogeneous, anisotropic scattering media of semi-infinite extent. Special attention is given to events associated with short path lengths. Empirical scaling relationships for path lengths and surface intensities are shown to agree with a consistency equation derived in an earlier study of anisotropic random walks. They are augmented here by a procedure that accounts for concomitant scaling of optical absorption coefficients. Results then are used to transform expressions that were obtained previously by analytical random-walk theory developed for an isotropic scattering model of photon migration. Quantities that are studied include the diffuse surface reflectance, the depth distribution of the fluence, and the time-resolved intensity of backreflected photons.

We describe a method for discriminating short- and long-path photons transmitted through a multiply scattering medium that is based on the relationship between the polarization states of the incident and forward-scattered light. Results of Monte Carlo simulations and experiments show that if the scattering anisotropy of the scatterers is sufficiently small, absorbing barriers embedded in optically dense suspensions of polystyrene spheres can be resolved with good contrast by selectively detecting a component of the scattered-light intensity that has preserved its incident circular polarization state. The principles of operation of a polarization-modulation system capable of measuring small polarization fractions are explained. Using this system we were able to measure polarized light in a depolarized background over 1000 times as large.

We describe a compact, non-contact design for a total emission detection (c-TED) system for intra-vital multiphoton imaging. To conform to a standard upright two-photon microscope design, this system uses a parabolic mirror surrounding a standard microscope objective in concert with an optical path that does not interfere with normal microscope operation. The non-contact design of this device allows for maximal light collection without disrupting the physiology of the specimen being examined. Tests were conducted on exposed tissues in live animals to examine the emission collection enhancement of the c-TED device compared to heavily optimized objective-based emission collection. The best light collection enhancement was seen from murine fat (5×-2× gains as a function of depth), whereas murine skeletal muscle and rat kidney showed gains of over two and just under twofold near the surface, respectively. Gains decreased with imaging depth (particularly in the kidney). Zebrafish imaging on a reflective substrate showed close to a twofold gain throughout the entire volume of an intact embryo (approximately 150 μm deep). Direct measurement of bleaching rates confirmed that the lower laser powers, enabled by greater light collection efficiency, yielded reduced photobleaching in vivo. The potential benefits of increased light collection in terms of speed of imaging and reduced photo-damage, as well as the applicability of this device to other multiphoton imaging methods is discussed. [ABSTRACT FROM AUTHOR]