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17. Detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in vaginal swabs of women with acute SARS‐CoV‐2 infection: a prospective study.

Author

Schwartz, A, Yogev, Y, Zilberman, A, Alpern, S, Many, A, Yousovich, R, and Gamzu, R

Subjects
COVID-19, SARS-CoV-2, THIRD trimester of pregnancy, NEONATAL infections, LONGITUDINAL method
Abstract

Objective: To determine whether severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is present in the vaginal secretions of both reproductive‐aged and postmenopausal women during acute SARS‐CoV‐2 infection. Design: Prospective study. Setting: A single tertiary, university‐affiliated medical centre in Israel. Time period, 1 June 2020 through to 31 July 2020. Population: Women who were hospitalised in a single tertiary medical centre, who were diagnosed with acute SARS‐CoV‐2 infection by a nasopharyngeal RT‐PCR test. Methods: Women were diagnosed with acute SARS‐CoV‐2 infection by a nasopharyngeal RT‐PCR test. Vaginal RT‐PCR swabs were obtained from all study participants after a proper cleansing of the perineum. Main outcome measures: Detection of SARS‐CoV‐2 in vaginal RT‐PCR swabs. Results: Vaginal and nasopharyngeal swabs were obtained from 35 women, aged 21–93 years. Twenty‐one women (60%) were in their reproductive years, of whom, five were in their third trimester of pregnancy. Most of the participants (57%) were healthy without any underlying medical conditions. Of the 35 patients sampled, 2 (5.7%) had a positive vaginal RT‐PCR for SARS‐CoV‐2, one was premenopausal and the other was a postmenopausal woman. Both women had mild disease. Conclusion: Our findings contradict most previous reports, which did not detect the presence of viral colonisation in the vagina. Although passage through the birth canal exposes neonates to the vaginal polymicrobial flora, an acquisition of pathogens does not necessarily mandate neonatal infection or clinical disease. Nevertheless, when delivering the infant of a woman with acute SARS‐CoV‐2 infection, a clinician should consider the possibility of vaginal colonisation, even if it is uncommon. When delivering the infant of a woman with acute SARS‐CoV‐2 infection, a clinician should consider the possibility of vaginal colonisation. When delivering the infant of a woman with acute SARS‐CoV‐2 infection, a clinician should consider the possibility of vaginal colonisation. [ABSTRACT FROM AUTHOR]

Published
2021
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18. Laboratory Challenges in Response to Silent Introduction and Sustained Transmission of Wild Poliovirus Type 1 in Israel During 2013

Author

Shulman, L. M., primary, Mendelson, E., additional, Anis, E., additional, Bassal, R., additional, Gdalevich, M., additional, Hindiyeh, M., additional, Kaliner, E., additional, Kopel, E., additional, Manor, Y., additional, Moran-Gilad, J., additional, Ram, D., additional, Sofer, D., additional, Somekh, E., additional, Tasher, D., additional, Weil, M., additional, Gamzu, R., additional, and Grotto, I., additional

Published
2014
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21. Insidious reintroduction of wild poliovirus into Israel, 2013

Author

Anis, E, primary, Kopel, E, additional, Singer, S R, additional, Kaliner, E, additional, Moerman, L, additional, Moran-Gilad, J, additional, Sofer, D, additional, Manor, Y, additional, Shulman, L M, additional, Mendelson, E, additional, Gdalevich, M, additional, Lev, B, additional, Gamzu, R, additional, and Grotto, I, additional

Published
2013
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49. Results of 6139 artificial insemination cycles with donor spermatozoa.

Author

Botchan, A., Hauser, R., Gamzu, R., Yogev, L., Paz, G., and Yavetz, H.

Abstract

Background: Artificial insemination by donor spermatozoa (AID) can prove a valuable treatment for a number of male factor disorders, although its success rate is variable.Methods: Retrospective analysis of the results of 6139 cycles performed in 1001 women during an 18 year period is presented. Pregnancy rates per cycle are presented as a function of: female fertility history, treatment modalities, medication used for induction of ovulation, female age, year of treatment, consecutive cycle effect and the use of fresh versus frozen-thawed spermatozoa.Results: Overall pregnancy rate of 12.6% and cumulative pregnancy rate after 12 months of treatment of 75% were achieved. Age was found to be the most important determinant for success rate.Conclusions: Since the establishment of AID treatments, the mean age of the population of women receiving treatment has increased each year. Consequently, success rate did not improve, even with the use of more sophisticated medical modalities. [ABSTRACT FROM AUTHOR]

Published
2001
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50. Genetic evaluation of infertile men.

Author

Kleiman, SE, Yogev, L, Gamzu, R, Hauser, R, Botchan, A, Lessing, JB, Paz, G, Yavetz, H, Kleiman, S E, and Lessing, J B

Abstract

Recently, microdeletions in the azoospermic factor region of the Y chromosome, in addition to chromosomal anomalies, have been detected in men with azoospermia or severe oligozoospermia. In this study we evaluated the molecular and cytogenetic defects of infertile men. The frequency of Y microdeletions among 105 azoospermic, 28 oligozoospermic and 32 fertile men was tested on lymphocyte DNA using a series of 20 sequence-tagged sites. In addition, microdeletions were evaluated on testicular-derived DNA among 26 azoospermic patients who underwent testicular biopsy and in whom no sperm cells could be identified. Karyotype analysis was performed on 72 of the infertile patients. Deletions were detected in 6.7% azoospermic and 3.6% oligozoospermic men. No deletions were identified among the fertile men. Identical results were obtained with DNA derived either from lymphocytes or testicular tissue. The frequency of chromosomal aberrations in the 72 infertile patients tested (62 azoospermic, 10 oligozoospermic) was 16.6%, with a high percentage of gonosome anomalies. Additional andrological parameters (hormone values, cryptorchidism) failed to identify men at risk for having microdeletions before the test. Our findings support the recommendation to perform genetic defect screening among infertile men before their enrollment in an intracytoplasmic injection/in-vitro fertilization programme. [ABSTRACT FROM AUTHOR]

Published
1999
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