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Your search keyword '"Gammoudi, Fatim T."' showing total 9 results
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9 results on '"Gammoudi, Fatim T."'

3. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

Author

Garbaj, Aboubaker M., Farag, Samira A., Sherif, Jihan A., Lawila, Aml F., Eshamah, Hanan L., Azwai, Salah M., Gammoudi, Fatim T., Naas, Hesham T., Salabi, Allaaeddin A. El, and Eldaghayes, Ibrahim M.

Subjects
Cronobacter sakazakii, Cronobacter pulveris, Thermo-tolerance, D˗value, z˗value, Infant milk formula
Abstract

Background: Cronobacter sspecies are the most significant foodborne pathogen in infant milk formula (IMF). These pathogens have been incriminated in severe forms of neonatal meningitis, sepsis, and necrotizing enterocolitis with a high mortality rate.Aim: This study was performed to elucidate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF).Methods: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C. The D- and z-values were determined by using linear regression analysis.Results: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5, and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, and 66°C were in the ranges 7.29–23.47, 2.77–15.50, 0.62–1.04, 0.62–1.02, 0.62–1.00, 0.62–1.00 minutes, respectively; while, the z˗values extended from 2.50°C to 4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) were in the ranges 7.60–22.32, 1.42–8.45, 0.62–1.08, 0.62–0.78, 0.62–0.78, 0.62–0.79 minutes at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33°C to 4.89°C.Conclusion: This study may contribute to improving the understanding of the behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperatures and may participate in the effective control of these pathogens in infant food production.

Published
2023

4. Occurrence and antibiogram of multidrug-resistant Salmonella enterica isolated from dairy products in Libya

Author

Garbaj, Aboubaker M., primary, Gawella, Tahani B. Ben, additional, Sherif, Jihan A., additional, Naas, Hesham T., additional, Eshamah, Hanan L., additional, Azwai, Salah M., additional, Gammoudi, Fatim T., additional, Abolghait, Said K., additional, Moawad, Ashraf A., additional, Barbieri, Ilaria, additional, and Eldaghayes, Ibrahim M., additional

Published
2022
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9. Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets.

Author

Naas HT, Edarhoby RA, Garbaj AM, Azwai SM, Abolghait SK, Gammoudi FT, Moawad AA, Barbieri I, and Eldaghayes IM

Abstract

Aim: The aim of the current investigation was to screen the presence of Staphylococci spp., especially S. aureus in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles., Materials and Methods: A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of Staphylococci spp. and S. aureus by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA., Results: Out of 139 samples, 112 (80.6%) were contaminated with different species of Staphylococci based on cultural characteristics of Staphylococci on Baird-Parker medium, for which S. aureus was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be S. aureus using the molecular technique. The six identified isolates of S. aureus were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be Methicillin-resistant Staphylococcus aureus ., Conclusion: Results of this study suggest that food of animal origin could be a source of S. aureus with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health.

Published
2019
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