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4. A questionnaire to collect unintended effects of transcranial magnetic stimulation:A consensus based approach

Author

Giustiniani, A., Vallesi, A., Oliveri, M., Tarantino, V., Ambrosini, E., Bortoletto, M., Masina, F., Busan, P., Siebner, H. R., Fadiga, L., Koch, G., Leocani, L., Lefaucheur, J. P., Rotenberg, A., Zangen, A., Violante, I. R., Moliadze, V., Gamboa, O. L., Ugawa, Y., Pascual-Leone, A., Ziemann, U., Miniussi, C., Burgio, F., Giustiniani, A., Vallesi, A., Oliveri, M., Tarantino, V., Ambrosini, E., Bortoletto, M., Masina, F., Busan, P., Siebner, H. R., Fadiga, L., Koch, G., Leocani, L., Lefaucheur, J. P., Rotenberg, A., Zangen, A., Violante, I. R., Moliadze, V., Gamboa, O. L., Ugawa, Y., Pascual-Leone, A., Ziemann, U., Miniussi, C., and Burgio, F.

Abstract

Transcranial magnetic stimulation (TMS) has been widely used in both clinical and research practice. However, TMS might induce unintended sensations and undesired effects as well as serious adverse effects. To date, no shared forms are available to report such unintended effects. This study aimed at developing a questionnaire enabling reporting of TMS unintended effects. A Delphi procedure was applied which allowed consensus among TMS experts. A steering committee nominated a number of experts to be involved in the Delphi procedure. Three rounds were conducted before reaching a consensus. Afterwards, the questionnaire was publicized on the International Federation of Clinical Neurophysiology website to collect further suggestions by the wider scientific community. A last Delphi round was then conducted to obtain consensus on the suggestions collected during the publicization and integrate them in the questionnaire. The procedure resulted in a questionnaire, that is the TMSens_Q, applicable in clinical and research settings. Routine use of the structured TMS questionnaire and standard reporting of unintended TMS effects will help to monitor the safety of TMS, particularly when applying new protocols. It will also improve the quality of data collection as well as the interpretation of experimental findings.

Published
2022

8. hTERT Protein Expression in Cytoplasm and Nucleus and its Association With HPV Infection in Patients With Cervical Cancer

Author

Moreno-Acosta, P, Molano, M, Morales, N, Acosta, J, Gonzalez-Prieto, C, Mayorga, D, Buitrago, L, Gamboa, O, Mejia, JC, Castro, J, Romero-Rojas, A, Espenel, S, Murray, GL, Garland, SM, Vallard, A, Magne, N, Moreno-Acosta, P, Molano, M, Morales, N, Acosta, J, Gonzalez-Prieto, C, Mayorga, D, Buitrago, L, Gamboa, O, Mejia, JC, Castro, J, Romero-Rojas, A, Espenel, S, Murray, GL, Garland, SM, Vallard, A, and Magne, N

Abstract

BACKGROUND: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. PATIENTS AND METHODS: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. RESULTS: hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8% had a nuclear localization). A statistically significant association was found between alpha 9 and 7 HPV species with a non-methylation pattern of the hTERT promoter and between these species and high expression of hTERT protein with nuclear localization. CONCLUSION: hTERT protein is found in both the nucleus and cytoplasm of patients with cervical cancer and confirm the relationship between the non-methylated status of hTERT promoter and some HPV species as well as the relationship between these species and hTERT protein expression.

Published
2020

18. Telomerase activity in cervical scrapes of women with high-grade cervical disease: A nested case-control study

Author

Molano, M, Martin, DC, Moreno-Acosta, P, Hernandez, G, Cornall, A, Buitrago, O, Gamboa, O, Garland, S, Tabrizi, S, Munoz, N, Molano, M, Martin, DC, Moreno-Acosta, P, Hernandez, G, Cornall, A, Buitrago, O, Gamboa, O, Garland, S, Tabrizi, S, and Munoz, N

Abstract

Epidemiological information on telomerase activity (TA) and development of cervical lesions is scarce. A nested case-control study was carried out within a cohort of Colombian women tested for Human Papillomavirus (HPV). Measurement of TA was done in cervical scrapes of 25 women who developed High Grade Squamous Intraepithelial Lesion (HGSIL) during the first 6 years of follow-up and was compared with that of 104 control women who maintained normal cytology during the entire follow-up. TA was measured by a telomerase repeat amplification protocol-ELISA. TA and HPV infections were significantly more frequent in cases than in controls. Likewise, 68% of the cases were positive for both TA and HPV compared with only 7.7% of the controls (P<0.0001). Factors independently associated with increased odds of HGSIL included TA, high risk HPV (hrHPV) infections and multiple parities. When restricted to hrHPV positive women, TA was strongly associated with increased odds of HGSIL (adjusted odds ratio=37.94, 95% confidence interval, 1.64-678.1). In addition to an infection with hrHPV, TA appears to be a significant cofactor for HGSIL.

Published
2018

25. Efecto de los niveles de grano de soya integral cocido sobre el desempeño zootécnico y la calidad del huevo en codornices (Coturnix coturnix japonica)

Author

GAMBOA, O. F, DÍAZ, J. C, HURTADO NERY, V. L, and GARZÓN, V

Subjects
Pseudoplatystoma fasciatum, bagre, bagre fish, cryoprotector, cryopreser vation, dimethyl sulfoxide, semen, crioconservación, dimetil sulfóxido, sperm
Abstract

Titulo en ingles: Effect of the grain of cooked integral soybean on egg prodution and egg quality in japanese quails (Coturnix coturnix japonica).RESUMEN: Esta investigación fue realizada para evaluar el efecto de la inclusión de grano de soya integral cocido en la preparación de raciones para la alimentación de codornices japonesas (Coturnix coturnix japonica) sobre el desempeño productivo y la calidad del huevo. Fueron utilizadas 250 codornices, de 30 días de edad, durante un periodo de 84 días, en un Diseño Experimental Completamente al Azar, distribuidas en cinco tratamientos, cinco niveles de Soya Integral Cocida (SIC), cinco repeticiones y 10 aves por repetición, para un total de 25 parcelas experimentales y 250 aves en el ensayo. Las raciones experimentales fueron formuladas con niveles crecientes de grano de soya (0%,5%, 10%, 15% y 20%). Los resultados de producción de huevos, peso de los huevos, consumo de alimento, conversión kg ración/ docena de huevos y kg ración/kg de huevos fueron para 0% SIC: 61.31%, 10.21g, 23g, 0,45 y 3.86;para 5% SIC: 63.64%, 0.61g, 22,85g, 0.43 y 3.45; 10% SIC: 60.31%, 10,25g, 23.46g, 0,46 y 3.96; 15% SIC: 60,19%, 10,63g, 22.87g, 0,45 y 3,73; 20 % SIC: 59.41%, 10,4g, 23,73g, 0,47 y 3.87 respectivamente. Las variables sobre calidad del huevo y de la cáscara, y grosor de la cáscara, Gravedad Específica y Unidades Haugh no presentaron diferencias significativas (P>0.05), cuyos valores fueron para 0% de SIC: 0,17mm, 1.070, 92,94; 5% de SIC: 0,18 mm, 1.073, 90,73; 10% de SIC 0,17mm, 1.072, 94,95; 15% de SIC: 0,17mm, 1.073, 92,94 e para 20% de SIC: 0,18mm, 1.071 y 92,25 respectivamente. En conclusión la inclusión de Soya Integral Cocida influye en el peso de los huevos y el consumo de ración, sinembargo, no tiene efecctos sobre la calidad del huevo.Palabras claves: codornices japonesas, grano de soya, conversión alimenticia, postura, Unidades Haugh.ABSTRACT: The research was driven to evaluate the effect of the inclusion of grain of integral soybean cooked in the preparation of diets for the feeding of Japanese quails (Coturnix coturnix japonica) on egg production and egg quality. 250 quails of 30 days of age were used, in a period of 84 days, distributed in five treatments, five levels of Cooked Soya Integral (SIC), with five repetitions for treatment 10 birds for repetition, for a total of 25 experimental parcels and 250 birds in the research. The diets balanced with growing contents of soybean (0%, 5%, 10%, 15% and 20%). The results of Eggs production, Weight of the Eggs, food consumption, Conversion kg intake / dozen of eggs and kg intake food/kg of eggs were for 0% SIC: 61.31%, 10.21g, 23g, 0,45 and 3.86; for 5% SIC: 63.64%, 10.61g, 22,85g, 0.43 and 3.45; 10% SIC: 60.31%, 10,25g, 23.46g, 0,46 and 3.96; 15% SIC: 60,19%, 10,63g, 22.87g, 0,45 and 3,73; 20% SIC:59.41%, 10,4g, 23,73g, 0,47 and 3.87; respectively. The caracteristics of the quality of the egg and of the shell, as thickness of the shell, Specific Graveness and Units Haugh, and, not had significant difference (P>0.05). The results were 0% de SIC: 0,17mm, 1.070, 92,94; 5% de SIC: 0,18mm, 1.073, 90,73;10% de SIC 0,17mm, 1.072, 94,95; 15% de SIC:,17mm, 1.073, 92,94 and 20% de SIC: 0,18mm, 1.071 and 92,25 respectively. The inclusion of cooked Integral Soya affect Weight of the Eggs, food consumption, thus doesn´t caracteristics of the qualityof the egg quails.Key words: Japanese quails, soybean grain, egg weight, nutritious conversion, Eggs production Titulo en ingles: Effect of the grain of cooked integral soybean on egg prodution and egg quality in japanese quails (Coturnix coturnix japonica).RESUMEN: Esta investigación fue realizada para evaluar el efecto de la inclusión de grano de soya integral cocido en la preparación de raciones para la alimentación de codornices japonesas (Coturnix coturnix japonica) sobre el desempeño productivo y la calidad del huevo. Fueron utilizadas 250 codornices, de 30 días de edad, durante un periodo de 84 días, en un Diseño Experimental Completamente al Azar, distribuidas en cinco tratamientos, cinco niveles de Soya Integral Cocida (SIC), cinco repeticiones y 10 aves por repetición, para un total de 25 parcelas experimentales y 250 aves en el ensayo. Las raciones experimentales fueron formuladas con niveles crecientes de grano de soya (0%,5%, 10%, 15% y 20%). Los resultados de producción de huevos, peso de los huevos, consumo de alimento, conversión kg ración/ docena de huevos y kg ración/kg de huevos fueron para 0% SIC: 61.31%, 10.21g, 23g, 0,45 y 3.86;para 5% SIC: 63.64%, 0.61g, 22,85g, 0.43 y 3.45; 10% SIC: 60.31%, 10,25g, 23.46g, 0,46 y 3.96; 15% SIC: 60,19%, 10,63g, 22.87g, 0,45 y 3,73; 20 % SIC: 59.41%, 10,4g, 23,73g, 0,47 y 3.87 respectivamente. Las variables sobre calidad del huevo y de la cáscara, y grosor de la cáscara, Gravedad Específica y Unidades Haugh no presentaron diferencias significativas (P>0.05), cuyos valores fueron para 0% de SIC: 0,17mm, 1.070, 92,94; 5% de SIC: 0,18 mm, 1.073, 90,73; 10% de SIC 0,17mm, 1.072, 94,95; 15% de SIC: 0,17mm, 1.073, 92,94 e para 20% de SIC: 0,18mm, 1.071 y 92,25 respectivamente. En conclusión la inclusión de Soya Integral Cocida influye en el peso de los huevos y el consumo de ración, sinembargo, no tiene efecctos sobre la calidad del huevo.Palabras claves: codornices japonesas, grano de soya, conversión alimenticia, postura, Unidades Haugh.ABSTRACT: The research was driven to evaluate the effect of the inclusion of grain of integral soybean cooked in the preparation of diets for the feeding of Japanese quails (Coturnix coturnix japonica) on egg production and egg quality. 250 quails of 30 days of age were used, in a period of 84 days, distributed in five treatments, five levels of Cooked Soya Integral (SIC), with five repetitions for treatment 10 birds for repetition, for a total of 25 experimental parcels and 250 birds in the research. The diets balanced with growing contents of soybean (0%, 5%, 10%, 15% and 20%). The results of Eggs production, Weight of the Eggs, food consumption, Conversion kg intake / dozen of eggs and kg intake food/kg of eggs were for 0% SIC: 61.31%, 10.21g, 23g, 0,45 and 3.86; for 5% SIC: 63.64%, 10.61g, 22,85g, 0.43 and 3.45; 10% SIC: 60.31%, 10,25g, 23.46g, 0,46 and 3.96; 15% SIC: 60,19%, 10,63g, 22.87g, 0,45 and 3,73; 20% SIC:59.41%, 10,4g, 23,73g, 0,47 and 3.87; respectively. The caracteristics of the quality of the egg and of the shell, as thickness of the shell, Specific Graveness and Units Haugh, and, not had significant difference (P>0.05). The results were 0% de SIC: 0,17mm, 1.070, 92,94; 5% de SIC: 0,18mm, 1.073, 90,73;10% de SIC 0,17mm, 1.072, 94,95; 15% de SIC:,17mm, 1.073, 92,94 and 20% de SIC: 0,18mm, 1.071 and 92,25 respectively. The inclusion of cooked Integral Soya affect Weight of the Eggs, food consumption, thus doesn´t caracteristics of the qualityof the egg quails.Key words: Japanese quails, soybean grain, egg weight, nutritious conversion, Eggs production

Published
2005

26. Avoiding a second amniocentesis to corroborate prenatal diagnosis by using refrigerated samples.

Author

Méndez-Rosado, L. A., Cuétara, E., Molina-Gamboa, O., Suarez-Mayedo, U., Huertas-Perez, G., Barrios-Martinez, A., Garcia, M., and Morales, E.

Subjects
AMNIOCENTESIS, PRENATAL diagnosis, AMNIOTIC liquid, FLUORESCENCE in situ hybridization, CHROMOSOME abnormalities, COLLECTION & preservation of biological specimens, COMPARATIVE studies, KARYOTYPES, RESEARCH methodology, MEDICAL cooperation, PRESERVATION of organs, tissues, etc., REFRIGERATION & refrigerating machinery, REOPERATION, RESEARCH, EVALUATION research, CASE-control method
Abstract

Objective: This study is aimed to probe the usefulness of refrigerated aliquots of amniotic fluid to be used for fluorescence in situ hybridization (FISH) in order to perform an accurate prenatal diagnosis avoiding the risk related to an additional amniocentesis procedure and the psychological stress to the pregnant woman and her family.Methods: Non-cultured amniotic fluid (AF) samples were analyzed by FISH. Such samples were divided into two groups. The first one included fresh collected AF (FCAF, N = 30). The second one included refrigerated samples of AF (RSAF, N = 12) to corroborate uncertain chromosome aberration identification obtained by conventional methods. Sample refrigeration did not exceed 18 days.Results: No differences were found between groups. In the RSAF group, three cases of chromosomal mosaicismo and seven cases of pseudomosaicism were corroborated. No alteration adjudicated to aberrant chromosomal line presence was found in born children according to genetic specialists' criteria. In the two remaining cases, applied procedure allowed elucidating fetal sex in one case and the origin of a marker chromosome in the other.Conclusions: Amniocytes obtained from RAFS are a useful biological material to be assayed by FISH, achieving an accurate prenatal diagnosis and avoiding an additional amniocentesis. [ABSTRACT FROM AUTHOR]

Published
2017
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42. Prevalence, clinical outcomes and rainfall association of acute respiratory infection by human metapneumovirus in children in Bogotá, Colombia

Author

Obando Evelyn, Fernández-Sarmiento Jaime, Montoya David, Acevedo Lorena, Arroyave Jenifer, and Gamboa Oscar

Subjects
Viral infection, Children, Respiratory syncytial virus, Pneumonia, Pediatrics, RJ1-570
Abstract

Abstract Background Acute respiratory infections (ARIs) are one of the main causes of morbidity and mortality in children. Viruses are the main etiological agents, and their behavior tends to be seasonal and vary by geographical location. Human metapneumovirus (HMPV) has recently been described as a cause of severe acute respiratory infection and its prevalence and clinical behavior in children at moderate altitudes is unknown. Methods A cross-sectional study was carried out on patients seen at a university hospital in Bogotá, Colombia between October 2015 and December 2017 in a city at a moderate altitude above sea level. Children with acute respiratory infections who had had a multiplex RT-PCR assay were selected. The prevalence of HMPV infection, its clinical outcomes and its relationship to rainfall were evaluated. Results Out of a total of 14,760 discharged patients, multiplex RT-PCR was performed on 502 and a virus was detected in 420 children with acute respiratory infection (ARI). The study group had a median age of 21 months (IQR 7–60), with similar proportion of males and females (56.4 and 43.6% respectively) and 5.2% (CI 95 3.3–7.8%) prevalence of HMPV infection. The group with HMPV infection showed a greater frequency of viral coinfection (22.7% vs 14% P = 0.03) compared with ARI caused by other viruses. The rate of bacterial coinfection (P = 0.31), presence of comorbidities (p = 0.75), length of hospital stay (P = 0.42), need for mechanical ventilation (P = 0.75) and mortality (P = 0.22) were similar for HMPV and other viral infections. A moderate correlation was established between HMPV infection and rainfall peaks (Spearman’s Rho 0.44 p = 0.02). Conclusions Human metapneumovirus was the fifth most frequently isolated virus in children with ARI, had similar clinical behavior and severity to other viruses but a higher rate of viral coinfection. Its peaks seem to correlate to rainy seasons.

Published
2019
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