129 results on '"Gambelli P"'
Search Results
2. Platinum-induced upregulation of ITGA6 promotes chemoresistance and spreading in ovarian cancer
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Gambelli, Alice, Nespolo, Anna, Rampioni Vinciguerra, Gian Luca, Pivetta, Eliana, Pellarin, Ilenia, Nicoloso, Milena S, Scapin, Chiara, Stefenatti, Linda, Segatto, Ilenia, Favero, Andrea, D’Andrea, Sara, Mucignat, Maria Teresa, Bartoletti, Michele, Lucia, Emilio, Schiappacassi, Monica, Spessotto, Paola, Canzonieri, Vincenzo, Giorda, Giorgio, Puglisi, Fabio, Vecchione, Andrea, Belletti, Barbara, Sonego, Maura, and Baldassarre, Gustavo
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- 2024
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3. Waste Biomass Pretreatments for Biogas Yield Optimization and for the Extraction of Valuable High-Added-Value Products: Possible Combinations of the Two Processes toward a Biorefinery Purpose
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Jessica Di Mario, Nicolò Montegiove, Alberto Maria Gambelli, Monica Brienza, Claudia Zadra, and Giovanni Gigliotti
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biomass pretreatments ,biogas ,biomethane ,biorefinery ,Biotechnology ,TP248.13-248.65 - Abstract
Second- and third-generation biorefineries enable the sustainable management of biomasses within the framework of circular economy principles. This approach aims to minimize waste biomass while generating high-value molecules and bio-energy, such as biogas. Biogas production is achieved via anaerobic digestion, a process where microorganisms metabolize organic compounds in the absence of oxygen to primarily produce CO2 and CH4. The efficiency of this process is closely linked to the composition of the biomass and, sometimes, characteristics of the initial matrix can impede the process. To address these challenges, various pretreatments are employed to enhance digestion efficiency and mitigate issues associated with biomass complexity. However, the implementation of pretreatments can be energy-intensive and costly. The extraction of valuable molecules from biomass for various applications can represent a form of pretreatment. This extraction process selectively removes recalcitrant molecules such as lignin and cellulose, which can hinder biodegradation, thereby adding new value to the biomass. These extracted molecules not only contribute to improved anaerobic digestion efficiency but also offer potential economic benefits by serving as valuable inputs across diverse industrial sectors. This article presents a detailed state of the art of the most widespread biomass pretreatments and specifies when biomass is pretreated to improve the biogas yield and, in contrast, when it is treated to extract high-added-value products. Finally, in order to define if the same treatment can be simultaneously applied for both goals, an experimental section was dedicated to the production of biogas from untreated olive mill wastewater and the same biomass after being freeze-dried and after the extraction of polyphenols and flavonoids. The use of pretreated biomass effectively improved the biogas production yield: the untreated olive mill wastewater led to the production of 147 mL of biogas, while after freeze-drying and after polyphenols/flavonoids extraction, the production was, respectively, equal to 169 mL and 268 mL of biogas.
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- 2024
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4. Author Correction: Platinum-induced upregulation of ITGA6 promotes chemoresistance and spreading in ovarian cancer
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Gambelli, Alice, Nespolo, Anna, Rampioni Vinciguerra, Gian Luca, Pivetta, Eliana, Pellarin, Ilenia, Nicoloso, Milena S, Scapin, Chiara, Stefenatti, Linda, Segatto, Ilenia, Favero, Andrea, D’Andrea, Sara, Mucignat, Maria Teresa, Bartoletti, Michele, Lucia, Emilio, Schiappacassi, Monica, Spessotto, Paola, Canzonieri, Vincenzo, Giorda, Giorgio, Puglisi, Fabio, Vecchione, Andrea, Belletti, Barbara, Sonego, Maura, and Baldassarre, Gustavo
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- 2024
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5. Unexpected impairment of INa underpins reentrant arrhythmias in a knock-in swine model of Timothy syndrome
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Porta-Sánchez, Andreu, Mazzanti, Andrea, Tarifa, Carmen, Kukavica, Deni, Trancuccio, Alessandro, Mohsin, Muhammad, Zanfrini, Elisa, Perota, Andrea, Duchi, Roberto, Hernandez-Lopez, Kevin, Jáuregui-Abularach, Miguel Eduardo, Pergola, Valerio, Fernandez, Eugenio, Bongianino, Rossana, Tavazzani, Elisa, Gambelli, Patrick, Memmi, Mirella, Scacchi, Simone, Pavarino, Luca F., Franzone, Piero Colli, Lentini, Giovanni, Filgueiras-Rama, David, Galli, Cesare, Santiago, Demetrio Julián, and Priori, Silvia G.
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- 2023
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6. Platinum-induced upregulation of ITGA6 promotes chemoresistance and spreading in ovarian cancer
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Alice Gambelli, Anna Nespolo, Gian Luca Rampioni Vinciguerra, Eliana Pivetta, Ilenia Pellarin, Milena S Nicoloso, Chiara Scapin, Linda Stefenatti, Ilenia Segatto, Andrea Favero, Sara D’Andrea, Maria Teresa Mucignat, Michele Bartoletti, Emilio Lucia, Monica Schiappacassi, Paola Spessotto, Vincenzo Canzonieri, Giorgio Giorda, Fabio Puglisi, Andrea Vecchione, Barbara Belletti, Maura Sonego, and Gustavo Baldassarre
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Ovarian Cancer ,Metastasis ,Integrin ,chemoresistance ,Medicine (General) ,R5-920 ,Genetics ,QH426-470 - Abstract
Abstract Platinum (PT)-resistant Epithelial Ovarian Cancer (EOC) grows as a metastatic disease, disseminating in the abdomen and pelvis. Very few options are available for PT-resistant EOC patients, and little is known about how the acquisition of PT-resistance mediates the increased spreading capabilities of EOC. Here, using isogenic PT-resistant cells, genetic and pharmacological approaches, and patient-derived models, we report that Integrin α6 (ITGA6) is overexpressed by PT-resistant cells and is necessary to sustain EOC metastatic ability and adhesion-dependent PT-resistance. Using in vitro approaches, we showed that PT induces a positive loop that, by stimulating ITGA6 transcription and secretion, contributes to the formation of a pre-metastatic niche enabling EOC cells to disseminate. At molecular level, ITGA6 engagement regulates the production and availability of insulin-like growth factors (IGFs), over-stimulating the IGF1R pathway and upregulating Snail expression. In vitro data were recapitulated using in vivo models in which the targeting of ITGA6 prevents PT-resistant EOC dissemination and improves PT-activity, supporting ITGA6 as a promising druggable target for EOC patients.
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- 2024
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7. Biomaterials for extrusion-based bioprinting and biomedical applications
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Arianna Rossi, Teresa Pescara, Alberto Maria Gambelli, Francesco Gaggia, Amish Asthana, Quentin Perrier, Giuseppe Basta, Michele Moretti, Nicola Senin, Federico Rossi, Giuseppe Orlando, and Riccardo Calafiore
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bioprinting ,material extrusion ,biomaterials ,biomedical applications ,artificial tissues ,Biotechnology ,TP248.13-248.65 - Abstract
Amongst the range of bioprinting technologies currently available, bioprinting by material extrusion is gaining increasing popularity due to accessibility, low cost, and the absence of energy sources, such as lasers, which may significantly damage the cells. New applications of extrusion-based bioprinting are systematically emerging in the biomedical field in relation to tissue and organ fabrication. Extrusion-based bioprinting presents a series of specific challenges in relation to achievable resolutions, accuracy and speed. Resolution and accuracy in particular are of paramount importance for the realization of microstructures (for example, vascularization) within tissues and organs. Another major theme of research is cell survival and functional preservation, as extruded bioinks have cells subjected to considerable shear stresses as they travel through the extrusion apparatus. Here, an overview of the main available extrusion-based printing technologies and related families of bioprinting materials (bioinks) is provided. The main challenges related to achieving resolution and accuracy whilst assuring cell viability and function are discussed in relation to specific application contexts in the field of tissue and organ fabrication.
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- 2024
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8. Olive Pomace Protein Hydrolysate Waste Valorization Through Biogas Production: Evaluation of Energy Produced and Process Efficiency
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Niccolò Montegiove, Alberto Maria Gambelli, Eleonora Calzoni, Agnese Bertoldi, Carla Emiliani, and Giovanni Gigliotti
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The biogas production from biomasses for energy production, must be considered as last step in the ideal biorefinery supply chain, since the extraction of valuable subproducts has greater importance and must be performed earlier than the final preparation of bio-digestates. This study aims to prove the technical feasibility of carrying out both these processes, or the initial extraction of valuable subproducts and the following production of biogas. Moreover, it aims to quantify the benefits introduced, in terms of biogas production, related to the addition of pomace to the digestate inoculation. For the scope, biogas was produced, in lab-scale apparatuses, starting from different digestate samples; among them, one was enriched with untreated pomace and another with the treated one, consisting of the residual of pomace used for sub-products extraction. Based on the measured quantity of biogas achieved, the energy produced was quantified; conversely, the energy consumed was evaluated according to the plant efficiencies declared in literature. The daily energy production and consumption were compared to define the optimal production period and, according to it, the quantity of energy lost, referred to the unextracted biogas from digestates, was quantified and the efficiency was re-defined. The benefits related to the addition of pomace and, in particular, treated pomace, were defined as a function of the overall energy production, the percentage of energy lost (or produced outside from the optimal production period) and the re-defined process efficiency. Finally, the energy obtainable per unit of dry matter and volatile solids was calculated.
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- 2024
9. Legumes and common beans in sustainable diets: nutritional quality, environmental benefits, spread and use in food preparations
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Silvia Lisciani, Stefania Marconi, Cinzia Le Donne, Emanuela Camilli, Altero Aguzzi, Paolo Gabrielli, Loretta Gambelli, Karl Kunert, Diana Marais, Barend Juan Vorster, Katherine Alvarado-Ramos, Emmanuelle Reboul, Eleonora Cominelli, Chiara Preite, Francesca Sparvoli, Alessia Losa, Tea Sala, Anna-Maria Botha, and Marika Ferrari
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sustainable diets ,legumes ,common beans ,traditional recipes ,plant-based diets ,Nutrition. Foods and food supply ,TX341-641 - Abstract
In recent decades, scarcity of available resources, population growth and the widening in the consumption of processed foods and of animal origin have made the current food system unsustainable. High-income countries have shifted towards food consumption patterns which is causing an increasingly process of environmental degradation and depletion of natural resources, with the increased incidence of malnutrition due to excess (obesity and non-communicable disease) and due to chronic food deprivation. An urgent challenge is, therefore, to move towards more healthy and sustainable eating choices and reorientating food production and distribution to obtain a human and planetary health benefit. In this regard, legumes represent a less expensive source of nutrients for low-income countries, and a sustainable healthier option than animal-based proteins in developed countries. Although legumes are the basis of many traditional dishes worldwide, and in recent years they have also been used in the formulation of new food products, their consumption is still scarce. Common beans, which are among the most consumed pulses worldwide, have been the focus of many studies to boost their nutritional properties, to find strategies to facilitate cultivation under biotic/abiotic stress, to increase yield, reduce antinutrients contents and rise the micronutrient level. The versatility of beans could be the key for the increase of their consumption, as it allows to include them in a vast range of food preparations, to create new formulations and to reinvent traditional legume-based recipes with optimal nutritional healthy characteristics.
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- 2024
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10. CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state
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McLaren, Mathew, Conners, Rebecca, Isupov, Michail N., Gil-Díez, Patricia, Gambelli, Lavinia, Gold, Vicki A. M., Walter, Andreas, Connell, Sean R., Williams, Bryony, and Daum, Bertram
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- 2023
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11. CO2 Storage in Deep Oceanic Sediments in the form of Hydrates: Energy Evaluation and Advantages Related to the Use of N2-Containing Mixtures
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Alessia Di Giuseppe and Alberto Maria Gambelli
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CO2 storage ,CO2 hydrates ,energy spent for storage ,CCUS ,flue gas mixtures ,Technology - Abstract
Permanent storage in suitable geological sites and/or deep aquifers is emerging as the most concrete and effective solution to mitigate its increasing concentration in the atmosphere. This article experimentally investigated its storage in deep marine environments in the form of hydrates. Gas hydrates were formed into a small-scale reactor, designed to reproduce marine seafloors. Hydrates were formed with pure carbon dioxide and with CO2-based gaseous mixture containing nitrogen at different concentrations, equal, respectively, to 30, 50 and 60 vol%. The results obtained for each mixture were then compared to each other. In particular, the quantity of hydrates formed was evaluated as a function of the thermodynamic conditions selected for the experiments. The energy spent for the process, calculated by considering the cooling and compression phases, was calculated for the unit quantity of hydrates formed and for the unit quantity of carbon dioxide stored. Finally, the energy requirements for gas cooling and for gas compression were calculated separately in order to comprehend the contribution of the single process for each mixture.
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- 2024
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12. The role of stakeholder involvement in EU research and innovation policy: a case study of Technology Platform Organics
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Gambelli, Danilo, Vairo, Daniela, Cuoco, Eduardo, and Zanoli, Raffaele
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- 2023
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13. Author Correction: Platinum-induced upregulation of ITGA6 promotes chemoresistance and spreading in ovarian cancer
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Alice Gambelli, Anna Nespolo, Gian Luca Rampioni Vinciguerra, Eliana Pivetta, Ilenia Pellarin, Milena S Nicoloso, Chiara Scapin, Linda Stefenatti, Ilenia Segatto, Andrea Favero, Sara D’Andrea, Maria Teresa Mucignat, Michele Bartoletti, Emilio Lucia, Monica Schiappacassi, Paola Spessotto, Vincenzo Canzonieri, Giorgio Giorda, Fabio Puglisi, Andrea Vecchione, Barbara Belletti, Maura Sonego, and Gustavo Baldassarre
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Medicine (General) ,R5-920 ,Genetics ,QH426-470 - Published
- 2024
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14. Unraveling the Role of Amino Acid L-Tryptophan Concentration in Enhancing CO2 Hydrate Kinetics
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Yan Li, Alberto Maria Gambelli, Yizhi Rao, Xuejian Liu, Zhenyuan Yin, and Federico Rossi
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CO2 hydrate ,formation kinetics ,L-tryptophan ,amino acid ,CO2 hydrate morphology ,hydrate-based CO2 sequestration ,Technology - Abstract
Carbon dioxide (CO2) hydrates have garnered significant interest as a promising technology for CO2 capture and storage due to its high storage capacity and moderate operating conditions. The kinetics of CO2 hydrate formation is a critical factor in determining the feasibility of hydrate-based CO2 capture and storage technologies. This study systematically investigates the promotional effects of the amino acid L-tryptophan (L-trp) on CO2 hydrate formation kinetics and morphology under stirred and unstirred conditions. In the stirred system, experiments were conducted in a high-pressure 100 mL reactor with 0.05, 0.10, and 0.30 wt% L-trp solution. CO2 gas uptake kinetics and morphological evolution were monitored using a high-resolution digital camera. Results showed that L-trp promoted CO2 hydrate formation kinetics without delay, with rapid CO2 consumption upon nucleation. Morphological evolution revealed rapid hydrate formation, wall-climbing growth, and dendritic morphology filling the bulk solution. Under unstirred conditions, experiments were performed in a larger 1 L reactor with 0.1 wt% and 0.5 wt% L-trp solutions to assess the influence of additive concentration on hydrate formation thermodynamics and kinetics. Results demonstrated that L-trp influenced both thermodynamics and kinetics of CO2 hydrate formation. Thermodynamically, 0.1 wt% L-trp resulted in the highest hydrate formation, indicating an optimal concentration for thermodynamic promotion. Kinetically, increasing L-trp concentration from 0.1 wt% to 0.5 wt% reduced formation time, demonstrating a proportional relationship between L-trp concentration and formation kinetics. These findings provide insights into the role of L-trp in promoting CO2 hydrate formation and the interplay between additive concentration, thermodynamics, and kinetics. The results can inform the development of effective hydrate-based technologies for CO2 sequestration, highlighting the potential of amino acids as promoters in gas hydrate.
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- 2024
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15. Assessing the Aging Effect on Ti/Au Bilayers for Transition-Edge Sensor (TES) Detectors
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Maria Gambelli, Matteo D’Andrea, Rita Asquini, Alessio Buzzin, Claudio Macculi, Guido Torrioli, and Sara Cibella
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TES ,Ti/Au ,aging effect ,proximity effect ,annealing ,superconductivity ,Chemical technology ,TP1-1185 - Abstract
Transition-edge sensor (TES) microcalorimeters are advanced cryogenic detectors that use a superconducting film for particle or photon detection. We are establishing a new production line for TES detectors to serve as cryogenic anticoincidence (i.e., veto) devices. These detectors are made with a superconducting bilayer of titanium (Ti) and gold (Au) thin films deposited via electron beam evaporation in a high vacuum condition on a monocrystalline silicon substrate. In this work, we report on the development of such sensors, aiming to achieve stable sensing performance despite the effects of aging. For this purpose, patterned and non-patterned Ti/Au bilayer samples with varying geometries and thicknesses were fabricated using microfabrication technology. To characterize the detectors, we present and discuss initial results from repeated resistance–temperature (R–T) measurements over time, conducted on different samples, thereby augmenting existing literature data. Additionally, we present a discussion of the sensor’s degradation over time due to aging effects and test a potential remedy based on an easy annealing procedure. In our opinion, this work establishes the groundwork for our new TES detector production line.
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- 2024
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16. Cold Energy Storage via Hydrates Production with Pure CO2 and CO2/N2 (70/30 and 50/50 vol%) Mixtures: Quantification and Comparison between Energy Stored and Energy Spent
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Alberto Maria Gambelli, Federico Rossi, and Giovanni Gigliotti
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gas hydrates ,carbon capture and storage ,cold energy storage ,carbon dioxide ,Technology - Abstract
Gas hydrates represent an attractive opportunity for gas storage. These ice-like structures can be produced both for the final disposal of greenhouse gases such as carbon dioxide in the solid form and for the storage of energy gases, such as methane, propane, and others, with the possibility of reaching energy densities comparable with those of pressurised vessels, but at lower pressures. In addition, gas hydrates can be directly produced for their capability to act as phase change materials at temperatures higher than 0 °C. This research deals with cold energy storage via the production of gas hydrate into a lab-scale apparatus. Hydrates were produced with pure carbon dioxide and with CO2/N2 mixtures (70/30 and 50/50 vol%). For each mixture, the amount of energy spent for hydrates production and cold energy stored were calculated, and the results were compared among each other. The addition of nitrogen to the system allowed us to maximise the energy stored/energy spent ratio, which passed from 78.06% to 109.04%; however, due to its molecular size and the consequent impossibility to stabilise the occupied water cages, nitrogen caused a reduction in the total quantity of hydrates produced, which was obviously proportional to the energy stored. Therefore, the concentration of nitrogen in the mixtures need to be carefully determined in order to optimise the Estored/Espent ratio.
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- 2024
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17. Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius
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Lavinia Gambelli, Mathew McLaren, Rebecca Conners, Kelly Sanders, Matthew C Gaines, Lewis Clark, Vicki AM Gold, Daniel Kattnig, Mateusz Sikora, Cyril Hanus, Michail N Isupov, and Bertram Daum
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archaea ,Sulfolobus ,S-layer ,cryoEM ,tomography ,single-particle analysis ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of Sulfolobus acidocaldarius. The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly.
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- 2024
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18. Comparison of nutritional and sensory quality of processed and unprocessed wild rocket leaves during cold storage
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Raffo, Antonio, Aguzzi, Altero, Baiamonte, Irene, Buonocore, Pasquale, Ferrari Nicoli, Stefano, Gambelli, Loretta, Moneta, Elisabetta, Nardo, Nicoletta, Peparaio, Marina, Ruggeri, Stefania, Sinesio, Fiorella, and Paoletti, Flavio
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- 2022
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19. Biogas Production with Residuals Deriving from Olive Mill Wastewater and Olive Pomace Wastes: Quantification of Produced Energy, Spent Energy, and Process Efficiency
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Nicolò Montegiove, Alberto Maria Gambelli, Eleonora Calzoni, Agnese Bertoldi, Debora Puglia, Claudia Zadra, Carla Emiliani, and Giovanni Gigliotti
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agri-food waste biomasses ,olive pomace ,biorefinery ,biogas ,biomethane ,circular economy ,Agriculture - Abstract
At present, taking into account the sustainability of the starting matrices, the biogas production industry is continuously growing, especially in consideration of ecological transition and circularity. The present study deals with the development of anaerobic bioreactors aimed at valorizing two specific wastes of the olive oil supply chain, i.e., the residual of protein hydrolysis process of three-phases olive pomace (OP-PH) and that recovered after the extraction of bioactive molecules from olive mill wastewater (OMWW waste). The energy consumed for biogas production varied from 0.52 kJ (OP and OMWW waste) to 0.97 kJ (OP-PH), while the energy produced for OP, OP-PH and OMMW waste was equal to 1.73, 2.94 and 1.60 kJ, respectively. The optimal production period was defined by considering only the range showing energy production higher than its consumption. According to this, OMWW showed the best performances, since it required 9 days (instead of 12 of untreated and treated OP) to reach the completion. The biogas production efficiency of the three-phase OP-PH waste calculated in the optimal production period, i.e., 12 days, was higher than the other samples, with a yield of 76.7% and a quantity of energy potentially producible corresponding to 1727.8 kJ/kg of volatile solids. These results pave the way for possible applications of this procedure for the planning of a multi-purpose biorefinery fed with by-products from the olive supply chain waste, thus promoting the use of sustainable waste materials from a circular economy perspective.
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- 2024
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20. Production of CH4/C3H8 (85/15 vol%) Hydrate in a Lab-Scale Unstirred Reactor: Quantification of the Promoting Effect Due to the Addition of Propane to the Gas Mixture
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Alberto Maria Gambelli, Giovanni Gigliotti, and Federico Rossi
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binary gas hydrates ,methane ,propane ,cage occupancy ,Technology - Abstract
By itself, propane is capable to form hydrates at extremely contained pressures, if compared with the values typical of “guests” such as methane and carbon dioxide. Therefore, its addition in mixtures with gases such as those previously mentioned is expected to reduce the pressure required for hydrate formation. When propane is mixed with carbon dioxide, the promoting effect cannot be observed since, due to their molecular size, these two molecules cannot fit in the same unit cell of hydrates. Therefore, each species produces hydrates independently from the other, and the beneficial effect is almost completely prevented. Conversely, if propane is mixed with methane, the marked difference in size, together with the capability of methane molecules to fit in the smaller cages of both sI and sII structures, will allow to form hydrates in thermodynamic conditions lower than those required for pure methane hydrates. This study aims to experimentally characterize such a synergistic and promoting effect, and to quantity it from a thermodynamic point of view. Hydrates were formed and dissociated within a silica porous sediment and the results were compared with the phase boundary equilibrium conditions for pure methane hydrates, defined according to experimental values available elsewhere in the literature. The obtained results were finally explained in terms of cage occupancy.
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- 2024
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21. Transient ischemic attacks in patients with active and occult cancer
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Morin Beyeler, Pasquale Castigliego, Joel Baumann, Victor Ziegler, Moritz Kielkopf, Madlaine Mueller, Stefan A. Bauer-Gambelli, Adnan Mujanovic, Thomas Raphael Meinel, Thomas Horvath, Urs Fischer, Johannes Kaesmacher, Mirjam R. Heldner, David Seiffge, Marcel Arnold, Thomas Pabst, Martin D. Berger, Babak B. Navi, Simon Jung, and Philipp Bücke
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transient ischemic attack ,malignancy ,biomarkers ,cerebro-vascular disorders ,D-dimer ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Background and aimParaneoplastic coagulopathy can present as stroke and is associated with specific biomarker changes. Identifying paraneoplastic coagulopathy can help guide secondary prevention in stroke patients, and early cancer detection might improve outcomes. However, unlike ischemic stroke, it remains unclear whether paraneoplastic coagulopathy is associated with transient ischemic attacks (TIA). This study assessed the presence of cancer-related biomarkers in TIA patients and evaluated long-term mortality rates in patients with and without active cancer.MethodsActive cancer was retrospectively identified in consecutive TIA patients treated at a comprehensive stroke center between 2015 and 2019. An association between the presence of cancer and cancer-related biomarkers was assessed using multivariable logistic regression. Long-term mortality after TIA was analyzed using multivariable Cox regression.ResultsAmong 1436 TIA patients, 72 had active cancer (5%), of which 17 were occult (1.2%). Cancer-related TIA was associated with male gender (adjusted odds ratio [aOR] 2.29, 95% CI 1.12–4.68), history of smoking (aOR 2.77, 95% CI 1.34–5.7), elevated D-dimer (aOR 1.77, 95% CI 1.26–2.49), lactate dehydrogenase (aOR 1.003, 95% CI 1.00–1.005), lower leukocyte count (aOR 1.20, 95% CI 1.04–1.38), and lower hemoglobin (aOR 1.02, 95% CI 1.00–1.04). Long-term mortality was associated with both active cancer (adjusted hazard ratios [aHR] 2.47, 95% CI 1.58–3.88) and occult cancer (aHR 3.08, 95% CI 1.30–7.32).ConclusionCancer-related TIA is not uncommon. Biomarkers known to be associated with cancer-related stroke also seem to be present in TIA patients. Early identification would enable targeted treatment strategies and could improve outcomes in this patient population.
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- 2023
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22. Prebiotic effect of sorghum biomass xylooligosaccharides employing immobilized endoxylanase from Thermomyces lanuginosus PC7S1T
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Nascimento, Andreza Gambelli Lucas Costa, Zuppa, Érica Sabrina, Zonin, Maiara, de Sousa, Gabriela Furlaneto Sanchez, de Andrades, Diandra, Ayub, Marco Antônio Záchia, Maller, Alexandre, da Conceição Silva, José Luis, de Cássia Garcia Simão, Rita, and Kadowaki, Marina Kimiko
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- 2022
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23. Gas Hydrates Formation for High – Efficiency Waste Water Treatment: Experimental Removal of NH4+ from Water via CO2 Hydrates Formation
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Alberto Maria Gambelli, Federico Rossi, and Giovanni Gigliotti
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Waste water treatment is an essential and mandatory step for most of the industrial processes involving water. It has been widely proved that the overconsumption of this source, together with the exponential growth of anthropogenic damaging processed, lowered the quality of groundwater and, more in general, water resources, at an alarming pace. The removal of salts, ions dissolved in water and other effluents, can be carried out through several methods. Membrane separation, evaporation and crystallization are the most applied for water recovery, while membrane, chemical precipitation, adsorption, ion exchange and freezing/thawing are the most effective techniques for heavy metal ion solutions. When applying these procedures, some critical aspects must be considered, mainly associated to removal efficiency, costs, availability and non-toxicity of the raw materials, environmental concerning and other. In this context, gas hydrates can be considered an alternative solution for water treatment, capable to overcome all the critical issues related to the traditional strategies. In particular, the formation of gas hydrates for ion removal from waste water, was proved to reach extremely high efficiencies; moreover, the removal efficiency was found to be proportional to the size of ions and to decrease with their charge. Based on these experimental considerations, this study deals with preliminary research on the removal of NH4+ from water. This species consists of one of the most affecting pollutants in bio – refineries and its removal is often energy intensive, with consequent high costs. Experiments were carried out in a lab – scale apparatus, where water was initially threated with ammonium chloride at specific concentration. Carbon dioxide was finally chosen for the formation of hydrates.
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- 2023
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24. Handgrip strength predicts length of hospital stay in an abdominal surgical setting: the role of frailty beyond age
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Marano, Luigi, Carbone, Ludovico, Poto, Gianmario Edoardo, Gambelli, Margherita, Nguefack Noudem, Leonelle Lore, Grassi, Giulia, Manasci, Fabiana, Curreri, Giulia, Giuliani, Alessandra, Piagnerelli, Riccardo, Savelli, Vinno, Marrelli, Daniele, Roviello, Franco, and Boccardi, Virginia
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- 2022
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25. An archaellum filament composed of two alternating subunits
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Gambelli, Lavinia, Isupov, Michail N., Conners, Rebecca, McLaren, Mathew, Bellack, Annett, Gold, Vicki, Rachel, Reinhard, and Daum, Bertram
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- 2022
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26. An archaellum filament composed of two alternating subunits
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Lavinia Gambelli, Michail N. Isupov, Rebecca Conners, Mathew McLaren, Annett Bellack, Vicki Gold, Reinhard Rachel, and Bertram Daum
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Science - Abstract
The archaellum is a molecular machine used by archaea to swim, consisting of an intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. Here, the authors use electron cryo-microscopy to elucidate the structure of an archaellum, and find that the filament is composed of two alternating archaellins.
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- 2022
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27. Evidence of Carotid Atherosclerosis Vulnerability Regression in Real Life From Magnetic Resonance Imaging: Results of the MAGNETIC Prospective Study
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Oronzo Catalano, Giulia Bendotti, Teresa L. Aloi, Alberto Ferrari Bardile, Mirella Memmi, Patrick Gambelli, Daniela Zanaboni, Alessandra Gualco, Emanuela Cattaneo, Antonio Mazza, Mauro Frascaroli, Esmeralda Eshja, Riccardo Bellazzi, Paolo Poggi, Giovanni Forni, and Maria Teresa La Rovere
- Subjects
cardiac rehabilitation ,carotid atherosclerosis ,magnetic resonance imaging ,modifiable risk factors ,secondary prevention ,vulnerability regression ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Atherosclerosis vulnerability regression has been evidenced mostly in randomized clinical trials with intensive lipid‐lowering therapy. We aimed to demonstrate vulnerability regression in real life, with a comprehensive quantitative method, in patients with asymptomatic mild to moderate carotid atherosclerosis on a secondary prevention program. Methods and Results We conducted a single‐center prospective observational study (MAGNETIC [Magnetic Resonance Imaging as a Gold Standard for Noninvasive Evaluation of Atherosclerotic Involvement of Carotid Arteries]): 260 patients enrolled at a cardiac rehabilitation center were followed for 3 years with serial magnetic resonance imaging. Per section cutoffs (95th/5th percentiles) were derived from a sample of 20 consecutive magnetic resonance imaging scans: (1) lipid‐rich necrotic core: 26% of vessel wall area; (2) intraplaque hemorrhage: 12% of vessel wall area; and (3) fibrous cap: (a) minimum thickness: 0.06 mm, (b) mean thickness: 0.4 mm, (c) projection length: 11 mm. Patients with baseline magnetic resonance imaging of adequate quality (n=247) were classified as high (n=63, 26%), intermediate (n=65, 26%), or low risk (n=119, 48%), if vulnerability criteria were fulfilled in ≥2 contiguous sections, in 1 or multiple noncontiguous sections, or in any section, respectively. Among high‐risk patients, a conversion to any lower‐risk status was found in 11 (17%; P=0.614) at 6 months, in 16 (25%; P=0.197) at 1 year, and in 19 (30%; P=0.009) at 3 years. Among patients showing any degree of carotid plaque vulnerability, 21 (16%; P=0.014) were diagnosed at low risk at 3 years. Conclusions This study demonstrates with a quantitative approach that vulnerability regression is common in real life. A secondary prevention program can promote vulnerability regression in asymptomatic patients in the mid to long term.
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- 2023
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28. CCUS Strategies as Most Viable Option for Global Warming Mitigation
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Alberto Maria Gambelli
- Subjects
n/a ,Technology - Abstract
The decarbonization of energy-consuming industrial sectors is nowadays becoming one of the most relevant challenges to counteract global warming and the consequences related to it [...]
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- 2023
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29. Review on the Usage of Small-Chain Hydrocarbons (C2—C4) as Aid Gases for Improving the Efficiency of Hydrate-Based Technologies
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Alberto Maria Gambelli and Federico Rossi
- Subjects
gas hydrates ,hydrocarbons as guests ,hydrate-based technologies ,process efficiency ,Technology - Abstract
This review article aims to describe the main applications of gas hydrates in industrial processes and the related advantages and limitations. In particular, gas storage, energy storage, gas transportation, final disposal of greenhouse gases, desalination, wastewater treatments, food concentration, and other technologies are described in detail. Similarly, the benefits and disadvantages of the solutions, currently adopted to improve the process efficiency, are discussed in the text. A particular focus on the use of additives and their capability to intervene during the formation of hydrates and on the replacement process is provided. The second part of the article deals with the use of small-chain hydrocarbons as aid gases during formation, to improve the efficiency and the competitivity of hydrate-based processes. First, the thermodynamic properties of hydrates, containing only these compounds, are described. Then, based on a collection of experimental data available elsewhere in the literature, their effect on the hydrate formation process, when present in the mixture, is shown and detailed. Finally, direct and experimental applications of these gases during hydrate-based processes are described to definitively prove the possibility of solving, partially or completely, most of the main limiting problems for the diffusion of hydrate-based technologies.
- Published
- 2023
- Full Text
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30. Implantable loop recorder monitoring in inherited cardiomyopathies: yield of clinically relevant arrhythmias
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Trancuccio, A, primary, Kukavica, D, additional, Zvielli, G, additional, Marino, M, additional, Gambelli, P, additional, Memmi, M, additional, Bloise, R, additional, Napolitano, C, additional, Mazzanti, A, additional, and Priori, S G, additional
- Published
- 2023
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31. Unexpectedly high prevalence of structural genomic variations in the Long QT Syndrome: evidence for broader clinical uptake
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Mazzanti, A, primary, Memmi, M, additional, Gambelli, P, additional, Guarracino, A, additional, Colombi, B, additional, Nastoli, J, additional, Trancuccio, A, additional, Kukavica, D, additional, Marino, M, additional, Bloise, R, additional, Napolitano, C, additional, and Priori, S G, additional
- Published
- 2023
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32. The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
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Lavinia Gambelli, Rob Mesman, Wouter Versantvoort, Christoph A. Diebolder, Andreas Engel, Wiel Evers, Mike S. M. Jetten, Martin Pabst, Bertram Daum, and Laura van Niftrik
- Subjects
Methylomirabilis ,NC10 phylum ,anaerobic methane oxidation ,S-layer ,cell shape ,cryo-tomography ,Microbiology ,QR1-502 - Abstract
Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative surface protein layer (S-layer) was observed as the outermost cell layer of these bacteria. We hypothesized that this S-layer is the determining factor for their polygonal cell shape. Therefore, we enriched the S-layer from M. lanthanidiphila cells and through LC-MS/MS identified a 31 kDa candidate S-layer protein, mela_00855, which had no homology to any other known protein. Antibodies were generated against a synthesized peptide derived from the mela_00855 protein sequence and used in immunogold localization to verify its identity and location. Both on thin sections of M. lanthanidiphila cells and in negative-stained enriched S-layer patches, the immunogold localization identified mela_00855 as the S-layer protein. Using electron cryo-tomography and sub-tomogram averaging of S-layer patches, we observed that the S-layer has a hexagonal symmetry. Cryo-tomography of whole cells showed that the S-layer and the outer membrane, but not the peptidoglycan layer and the cytoplasmic membrane, exhibited the polygonal shape. Moreover, the S-layer consisted of multiple rigid sheets that partially overlapped, most likely giving rise to the unique polygonal cell shape. These characteristics make the S-layer of M. lanthanidiphila a distinctive and intriguing case to study.
- Published
- 2021
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33. Utilization of a Commercial 3D Printer for the Construction of a Bio-Hybrid Device Based on Bioink and Adult Human Mesenchymal Cells
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Giulio Morelli, Teresa Pescara, Alessia Greco, Pia Montanucci, Giuseppe Basta, Federico Rossi, Riccardo Calafiore, and Alberto Maria Gambelli
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3D printer ,cost and energy savings ,printing setup ,human mesenchymal stem cells ,biomaterials ,cellulose ,Technology - Abstract
The biofabrication of three-dimensional scaffolds using 3D printers and cell-containing bioinks is very promising. A wide range of materials and bioink compositions are being created and tested for cell viability and printability in order to satisfy the requirements of a bioink. This methodology has not still achieved technological maturity, and the actual costs mean that they are often inaccessible for researchers, consequently lowering the development and extending the required times. This research aims to apply this methodology on a laboratory scale by re-adapting a commercial 3D printer, consequently lowering the costs and energy impacts, and, at the same time, ensuring a level of accuracy extremely close to the currently adopted devices and, more in general, suitable for the scopes of the research. To accomplish this, we assembled a biomimetic scaffold made of human Umbilical Cord Matrix Stem Cells (hUCMS), cellulose, and alginate. Various molds were used to produce 3D scaffolds of different sizes. After bioprinting, cell viability was analyzed using ethidium bromide and fluorescein diacetate, and a histological stain was used to evaluate cell and bioink morphology. All of the examined bioinks had a uniform final 3D structure and were stable, easily printable, and procedure-adapted. Up until 21 days of culture, the bioinks remained unaltered and were simple to manipulate. After 7 and 21 days of cell culture, the hUCMS in the cellulose/alginate-based bioinks exhibited cell viabilities of 95% and 85%, respectively. The cells did not present with a fibroblast-like shape but appeared to be round-shaped and homogeneously distributed in the 3D structure. Biomimetic bioink, which is based on cellulose and alginate, is an appropriate hydrogel for 3D bioprinting. This preliminary work illustrated the potential use of these two biomaterials for the 3D bioprinting of mesenchymal stem cells.
- Published
- 2022
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34. Gas Hydrates as High-Efficiency Storage System: Perspectives and Potentialities
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Alberto Maria Gambelli, Federico Rossi, and Franco Cotana
- Subjects
green hydrogen ,hydrogen storage ,clathrate hydrates ,Technology - Abstract
The growing economic efforts and investment for the production of green hydrogen make the definition of new competitive and environmentally friendly storage methods. This article deals with the proposal of gas hydrate production with binary or ternary H2-based gaseous mixtures for hydrogen storage. In the text, the physical and chemical elements necessary to confirm the technical feasibility of the process are given. The proposed solution is also compared with the traditional ones in terms of energy costs, energy density, environmental sustainability, safety, ease of transport, future perspectives, and innovation level.
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- 2022
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35. Splicing factor proline- and glutamine-rich (SFPQ) protein regulates platinum response in ovarian cancer-modulating SRSF2 activity
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Pellarin, Ilenia, Dall’Acqua, Alessandra, Gambelli, Alice, Pellizzari, Ilenia, D’Andrea, Sara, Sonego, Maura, Lorenzon, Ilaria, Schiappacassi, Monica, Belletti, Barbara, and Baldassarre, Gustavo
- Published
- 2020
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36. Experimental Study on the Effect of SDS and Micron Copper Particles Mixture on Carbon Dioxide Hydrates Formation
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Yan Li, Alberto Maria Gambelli, and Federico Rossi
- Subjects
carbon dioxide hydrates ,solid additives ,kinetic promoter ,SDS ,micron Cu particles ,Technology - Abstract
CO2 hydrate formation and dissociation are the fundamental processes for investigating hydrate-based carbon storage. To better understand CO2 hydrate phase behaviors in the presence of surfactant and solid additives, this study reports the effects of Sodium Dodecyl Sulfate (SDS) and micron Cu particles on the formation of CO2 hydrates in the presence of porous quartz sands in a lab-scale reactor. This research is part of a wider study focused on defining the properties of solid additives, produced via gas-atomization, on the formation and dissociation of gas hydrates. The morphology of CO2 hydrate formed in SDS solution shows dispersed crystal particles due to the increase of surface tension. SDS works as the kinetic promoter on CO2 hydrates formation whereas the addition of Cu particles inhibits CO2 gas consumption. The mixture additives show a faint kinetic inhibit effect, in which the Brownian motion restrictions may be responsible for the inhibition of CO2 hydrate production. The solid additives also showed a weak thermodynamic effect on CO2 hydrate phase equilibrium.
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- 2022
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37. Hydrate formation as a method for natural gas separation into single compounds: a brief analysis of the process potential
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Gambelli, Alberto Maria and Rossi, Federico
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- 2021
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38. Formation and Dissociation of CH4 and CO2 Hydrates in Presence of a Sediment Composed by Pure Quartz Mixed with Ti23 Particles
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Alberto Maria Gambelli, Giulia Stornelli, Andrea Di Schino, and Federico Rossi
- Subjects
gas hydrates ,carbon dioxide storage ,solid additives ,kinetic promoters ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
The present research deals with the formation and dissociation of methane and carbon dioxide hydrates in a confined environment (small—size reactor) and in presence of a porous sediment of pure quartz impregnated with Ti23 particles. This research is part of a wider study aimed at verifying the possibility to use metallic powders, produced via gas-atomization for applications in additive manufacturing, as additives during the production/dissociation of gas hydrates. The porous medium was used to ensure the presence of Ti23 particles in the whole volume and not only in the lowest portion of the internal volume. For both the guest compounds considered, two Ti23 concentrations were explored, respectively, 8.68 and 26.04 wt%. Under the thermodynamic point of view, the dissociation process well approximated the phase equilibrium (defined with values collected from literature) for both compounds. In addition, the amount of gas trapped into hydrates, evaluated as a function of the initial amount of gas inserted inside the reactor, did not show relevant changes. Conversely, the presence of Ti23 was found to reduce the induction time for both components, thus allowing to define it as a kinetic promoter for the process. Such tendency was found to increase with the concentration.
- Published
- 2022
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39. Estimating missing data for organic farming by multiple imputation: the case of organic fruit yields in Italy
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Solfanelli, Francesco, Gambelli, Danilo, Vairo, Daniela, and Zanoli, Raffaele
- Published
- 2019
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40. Exploring Visitors’ Visual Behavior Using Eye-Tracking: The Case of the 'Studiolo Del Duca'
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Serena Mandolesi, Danilo Gambelli, Simona Naspetti, and Raffaele Zanoli
- Subjects
art exploration ,visual perception ,visual patterns ,eye-tracking ,visitor experience ,active vision ,Photography ,TR1-1050 ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Although the understanding of cognitive disciplines has progressed, we know relatively little about how the human brain perceives art. Thanks to the growing interest in visual perception, eye-tracking technology has been increasingly used for studying the interaction between individuals and artworks. In this study, eye-tracking was used to provide insights into non-expert visitors’ visual behaviour as they move freely in the historical room of the “Studiolo del Duca” of the Ducal Palace in Urbino, Italy. Visitors looked for an average of almost two minutes. This study revealed which parts of the artefact captured visitors’ attention and also gives interesting information about the main patterns of fruition.
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- 2022
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41. Methane and Carbon Dioxide Hydrate Formation and Dissociation in Presence of a Pure Quartz Porous Framework Impregnated with CuSn12 Metallic Powder: An Experimental Report
- Author
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Alberto Maria Gambelli, Giulia Stornelli, Andrea Di Schino, and Federico Rossi
- Subjects
natural gas hydrate ,carbon dioxide capture ,hydrate equilibrium ,chemical promoters ,CuSn12 metallic powder ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Hydrate formation and dissociation processes were carried out in the presence of a pure quartz porous medium impregnated with a metallic powder made with a CuSn12 alloy. Experiments were firstly made in the absence of that powder; then, different concentrations were added to the porous medium: 4.23 wt.%, 18.01 wt.%, and 30.66 wt.%. Then, the hydrate dissociation values were compared with those present in the literature. The porous medium was found to act as an inhibitor in the presence of carbon dioxide, while it did not alter methane hydrate, whose formation proceeded similarly to the ideal trend. The addition of CuSn12 promoted the process significantly. In particular, in concentrations of up to 18.01 wt.%, CO2 hydrate formed at milder conditions until it moved below the ideal equilibrium curve. For methane, the addition of 30.66 wt.% of powder significantly reduced the pressure required to form hydrate, but in every case, dissociation values remained below the ideal equilibrium curve.
- Published
- 2021
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42. The Effect of the Substrate on the Optic Performance of Retro-Reflective Coatings: An In-Lab Investigation
- Author
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Alessia Di Giuseppe, Marta Cardinali, Beatrice Castellani, Mirko Filipponi, Alberto Maria Gambelli, Lucio Postrioti, Andrea Nicolini, and Federico Rossi
- Subjects
Urban Heat Island ,retro-reflective coatings ,glass beads ,optic analysis ,angular reflectivity ,Technology - Abstract
Retro-reflectivity is a promising surface capability, which has attracted the interest of researchers for building applications in order to counteract Urban Heat Island (UHI) effects. This work aims at studying the impact of the substrate material on the optic performance of retro-reflective (RR) coatings. Three types of substrate materials were investigated: smooth pine wood panels, rough plywood panels, and smooth acetate sheets. The RR coating samples were made by firstly adding a high reflective white paint onto the substrate material and a homogeneous RR glass beads layer on the top. As a reference case, also diffusive samples, without RR beads, were developed. Samples have been tested through a spectrophotometric and an angular reflectivity analysis. Results show that, despite a lower global reflectance of the RR samples with respect to the diffusive ones, the glass beads coating provides a good retro-reflective capability to all the diffusive samples. Additionally, the roughest RR sample exhibited the highest RR capability of up to 16%, with respect to the other smoother samples. Future developments may involve the optimum design of RR coatings, in terms of their optic performance by varying the substrate materials and roughness, the glass beads density and dimension.
- Published
- 2021
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43. Observation of the Main Natural Parameters Influencing the Formation of Gas Hydrates
- Author
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Alberto Maria Gambelli, Umberta Tinivella, Rita Giovannetti, Beatrice Castellani, Michela Giustiniani, Andrea Rossi, Marco Zannotti, and Federico Rossi
- Subjects
water chemistry ,Antarctic sediment samples ,salinity ,gas hydrate ,Technology - Abstract
Chemical composition in seawater of marine sediments, as well as the physical properties and chemical composition of soils, influence the phase behavior of natural gas hydrate by disturbing the hydrogen bond network in the water-rich phase before hydrate formation. In this article, some marine sediments samples, collected in National Antarctic Museum in Trieste, were analyzed and properties such as pH, conductivity, salinity, and concentration of main elements of water present in the sediments are reported. The results, obtained by inductively coupled plasma-mass spectrometry (ICP-MS) and ion chromatography (IC) analysis, show that the more abundant cation is sodium and, present in smaller quantities, but not negligible, are calcium, potassium, and magnesium, while the more abundant anion is chloride and sulfate is also appreciable. These results were successively used to determine the thermodynamic parameters and the effect on salinity of water on hydrates’ formation. Then, hydrate formation was experimentally tested using a small-scale apparatus, in the presence of two different porous media: a pure silica sand and a silica-based natural sand, coming from the Mediterranean seafloor. The results proved how the presence of further compounds, rather than silicon, as well as the heterogeneous grainsize and porosity, made this sand a weak thermodynamic and a strong kinetic inhibitor for the hydrate formation process.
- Published
- 2021
- Full Text
- View/download PDF
44. Injection of CO2/N2 gaseous mixtures into gas hydrates to contemporary perform CH4 recovery and CO2 storage
- Author
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Filipponi Mirko, Gambelli Alberto Maria, Li Yan, Presciutti Andrea, Castellani Beatrice, Nicolini Andrea, Rossi Federico, and Cotana Franco
- Subjects
natural gas hydrates ,co2 capture ,flue-gas ,methane replacement ,Environmental sciences ,GE1-350 - Abstract
Carbon dioxide injection into natural gas hydrate reservoirs represents a promising opportunity to predispose a theoretically carbon neutral energy source. This technique allows to replace methane molecules with an equal number of carbon dioxide molecules and, consequently, to balance in advance emissions associated to methane utilization. While the direct CH4/CO2 replacement has been widely investigated, more data and scientific evidences are required to well define the feasibility of recovering methane by replacing it with CO2-based gaseous mixtures. In this sense, the most promising opportunity consists in flue-gas mixtures. In some cases, the presence of nitrogen was found capable to improve the overall efficiency, due to the direct competition between CH4 and N2 molecules to fill small cages characterizing hydrate structures. Moreover, these mixtures are extremely less-expensive than pure carbon dioxide. In this work, a binary CO2/N2 (50/50 vol%) gaseous mixture was used to recover methane contained into hydrate structures. Experiments were carried out in a small-scale experimental apparatus, designed to simulate a natural gas hydrate reservoir and to intervene on it with replacement techniques. Composition of gaseous mixtures present into hydrates and in the gaseous phase present immediately above, where defined via gas-chromatographic analyses. Finally, results were compared with data currently present in literature, in order to validate their consistency.
- Published
- 2021
- Full Text
- View/download PDF
45. Application of a completely organic and bio-degradable sugar-based insulating coating to vine shoots, to prevent late frost damages
- Author
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Di Giuseppe Alessia, Gambelli Alberto Maria, Palliotti Alberto, Nicolini Andrea, and Rossi Federico
- Subjects
Environmental sciences ,GE1-350 - Abstract
An innovative method against late frosts in vineyard has been developed at CIRIAF, University of Perugia. It consists of an organic coating made by cotton candy and straw to prevent the vine shoots freezing due to late frosts. This technique has already tested at lab-scale with very encouraging results. This paper aims to experiment the effect of the organic coating applied on young shoots of grapevine during artificially controlled frost events. A vine shoot covered by the organic coating was positioned in the climatic chamber and a thermocouple was rolled up to the vine shoot to control its temperature. Also, a vine shoot rolled up to the thermocouple and without organic coating was positioned inside the climatic chamber, used as a reference case. Results show that the temperature gap, gained with the adoption of the insulating layer was approximately in the range 0.2 – 3.2 °C, but the organic coating was not enough to maintain temperature above the ice-point able to prevent the vine shoot freezing in lab. Even thought, the experimental application of the present technique allowed to detect some crucial challenges which need to be solved before to test this technology in real situations.
- Published
- 2021
- Full Text
- View/download PDF
46. Olive Mill Wastes: A Source of Bioactive Molecules for Plant Growth and Protection against Pathogens
- Author
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Fabio Sciubba, Laura Chronopoulou, Daniele Pizzichini, Vincenzo Lionetti, Claudia Fontana, Rita Aromolo, Silvia Socciarelli, Loretta Gambelli, Barbara Bartolacci, Enrico Finotti, Anna Benedetti, Alfredo Miccheli, Ulderico Neri, Cleofe Palocci, and Daniela Bellincampi
- Subjects
Olea europaea L. ,olive mill wastes ,plant growth ,plant nutrition ,plant protection ,phenols ,Biology (General) ,QH301-705.5 - Abstract
Olive oil production generates high amounts of liquid and solid wastes. For a long time, such complex matrices were considered only as an environmental issue, due to their polluting properties. On the other hand, olive mill wastes (OMWs) exert a positive effect on plant growth when applied to soil due to the high content of organic matter and mineral nutrients. Moreover, OMWs also exhibit antimicrobial activity and protective properties against plant pathogens possibly due to the presence of bioactive molecules including phenols and polysaccharides. This review covers the recent advances made in the identification, isolation, and characterization of OMW-derived bioactive molecules able to influence important plant processes such as plant growth and defend against pathogens. Such studies are relevant from different points of view. First, basic research in plant biology may benefit from the isolation and characterization of new biomolecules to be potentially applied in crop growth and protection against diseases. Moreover, the valorization of waste materials is necessary for the development of a circular economy, which is foreseen to drive the future development of a more sustainable agriculture.
- Published
- 2020
- Full Text
- View/download PDF
47. Autologous platelet-rich plasma (PRP) in chronic penile lichen sclerosus: the impact on tissue repair and patient quality of life
- Author
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Casabona, Francesco, Gambelli, Ilaria, Casabona, Federica, Santi, Pierluigi, Santori, Gregorio, and Baldelli, Ilaria
- Published
- 2017
- Full Text
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48. A Building Energy Management System Based on an Equivalent Electric Circuit Model
- Author
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Giovanni Bianco, Stefano Bracco, Federico Delfino, Lorenzo Gambelli, Michela Robba, and Mansueto Rossi
- Subjects
building energy management system ,simulation ,optimization ,equivalent electric circuit ,Technology - Abstract
In recent decades, many EU and national regulations have been issued in order to increase the energy efficiency in different sectors and, consequently, to reduce environmental pollution. In the building sector, energy efficiency interventions are usually based on the use of innovative insulated materials and on the installation of cogeneration and tri-generation units, as well as solar technologies. New and retrofitted buildings are more and more commonly being called “smart buildings”, since they are characterized by the installation of electric and thermal power generation units, energy storage systems, and flexible loads; the presence of such technologies determines the necessity of installing Building Energy Management Systems (BEMSs), which are used to optimally manage their operation. The present paper proposes a BEMS for a smart building, equipped with plants based on renewables (photovoltaics, solar thermal panels, and geothermal heat pump), where the heating and cooling demand are satisfied by a Heating, Ventilation and Air Conditioning System (HVAC) fed by a geothermal heat pump. The developed BEMS is composed of two different modules: an optimization tool used to optimally manage the HVAC plant, in order to guarantee a desired level of comfort inside rooms, and a simulation tool, based on an equivalent electric circuit model and used to evaluate the thermal dynamic behavior of the building. The paper describes the two modules and shows the main results of the validation phase that has been conducted on a real test-case represented by the Smart Energy Building (SEB) located at the Savona Campus of the University of Genoa, Italy.
- Published
- 2020
- Full Text
- View/download PDF
49. Experimental analysis of the CO2/CH4 Replacement Efficiency due to Sodium Chloride Presence in Natural Gas Hydrates Reservoirs
- Author
-
Gambelli Alberto Maria, Castellani Beatrice, Filipponi Mirko, Nicolini Andrea, and Rossi Federico
- Subjects
natural gas hydrate ,co2/ch4 exchange ,chemical inhibitors injection ,sodium chloride effect ,Environmental sciences ,GE1-350 - Abstract
Nowadays natural gas hydrates represent a promising opportunity for counteracting several crucial issues of the 21th century. They are a valid answer to the continuously increasing energy demand, moved by the global population growth; moreover, considering their conformation and the possibility of using them for carbon dioxide permanently storage, gas hydrates may become a carbon neutral energy source, where for each methane molecule recovered, another carbon dioxide molecule is entrapped in solid form. Considering that the combustion of one methane molecule for energy production leads to the formation of one CO2 molecule, the hydrates exploitation can be considered a clean process in terms of impact on the climate change. This work shows how the presence of sodium chloride affects the CO2/CH4 replacement process into a gas hydrates reservoir. Replacement experimental results carried out in pure demineralised water were compared with the same values performed in a mixture of water and salt, having a concentration of 37 g/l. Some parameters of interest were discussed, such us methane hydrates formed before the replacement process, total amount of hydrates (composed by both species) reached at the end of the whole process, CO2 moles that formed hydrate, quantity of hydrate present before the replacement process which were actually involved in the CO2/CH4 exchange and carbon dioxide amount which led to the formation of new hydrates structures.
- Published
- 2020
- Full Text
- View/download PDF
50. Comparative Genomics of Candidatus Methylomirabilis Species and Description of Ca. Methylomirabilis Lanthanidiphila
- Author
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Wouter Versantvoort, Simon Guerrero-Cruz, Daan R. Speth, Jeroen Frank, Lavinia Gambelli, Geert Cremers, Theo van Alen, Mike S. M. Jetten, Boran Kartal, Huub J. M. Op den Camp, and Joachim Reimann
- Subjects
methylomirabilis ,anaerobic methane oxidation ,NC10 ,nitrite ,methylotrophy ,Microbiology ,QR1-502 - Abstract
Methane is a potent greenhouse gas, which can be converted by microorganism at the expense of oxygen, nitrate, nitrite, metal-oxides or sulfate. The bacterium ‘Candidatus Methylomirabilis oxyfera,’ a member of the NC10 phylum, is capable of nitrite-dependent anaerobic methane oxidation. Prolonged enrichment of ‘Ca. M. oxyfera’ with cerium added as trace element and without nitrate resulted in the shift of the dominant species. Here, we present a high quality draft genome of the new species ‘Candidatus Methylomirabilis lanthanidiphila’ and use comparative genomics to analyze its metabolic potential in both nitrogen and carbon cycling. To distinguish between gene content specific for the ‘Ca. Methylomirabilis’ genus and the NC10 phylum, the genome of a distantly related NC10 phylum member, CSP1-5, an aerobic methylotroph, is included in the analysis. All genes for the conversion of nitrite to N2 identified in ‘Ca. M. oxyfera’ are conserved in ‘Ca. M. lanthanidiphila,’ including the two putative genes for NO dismutase. In addition both species have several heme-copper oxidases potentially involved in NO and O2 respiration. For the oxidation of methane ‘Ca. Methylomirabilis’ species encode a membrane bound methane monooxygenase. CSP1-5 can act as a methylotroph, but lacks the ability to activate methane. In contrast to ‘Ca. M. oxyfera,’ which harbors three methanol dehydrogenases (MDH), both CSP1-5 and ‘Ca. M. lanthanidiphila’ only encode a lanthanide-dependent XoxF-type MDH, once more underlining the importance of rare earth elements for methylotrophic bacteria. The pathways for the subsequent oxidation of formaldehyde to carbon dioxide and for the Calvin–Benson–Bassham cycle are conserved in all species. Furthermore, CSP1-5 can only interconvert nitrate and nitrite, but lacks subsequent nitrite or NO reductases. Thus, it appears that although the conversion of methanol to carbon dioxide is present in several NC10 phylum bacteria, the coupling of nitrite reduction to the oxidation of methane is a trait so far unique to the genus ‘Ca. Methylomirabilis.’
- Published
- 2018
- Full Text
- View/download PDF
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