43 results on '"Gama, Florinda"'
Search Results
2. Isolation and Selection of Protein-Rich Mutants of Chlorella vulgaris by Fluorescence-Activated Cell Sorting with Enhanced Biostimulant Activity to Germinate Garden Cress Seeds.
- Author
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Trovão, Mafalda, Schüler, Lisa, Pedroso, Humberto, Reis, Ana, Santo, Gonçalo Espírito, Barros, Ana, Correia, Nádia, Ribeiro, Joana, Bombo, Gabriel, Gama, Florinda, Viana, Catarina, Costa, Monya M., Ferreira, Sara, Cardoso, Helena, Varela, João, Silva, Joana, Freitas, Filomena, and Pereira, Hugo
- Subjects
MUTANT proteins ,BIOLOGICAL products ,MUTAGENESIS ,BIOMASS ,AGRICULTURE - Abstract
Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutagenesis was performed with Chlorella vulgaris, and a selection of protein-rich mutants were sorted through fluorescence-activated cell sorting (FACS), resulting in the isolation of 17 protein-rich mutant strains with protein contents 19–34% higher than that of the wildtype (WT). Furthermore, mutant F4 displayed a 38%, 22% and 62% higher biomass productivity, growth rate and chlorophyll content, respectively. This mutant was then scaled up to a 7 L benchtop reactor to produce biomass and evaluate the biostimulant potential of this novel strain towards garden cress seeds. Compared to water (control), the germination index and the relative total growth increased by 7% and 19%, respectively, after the application of 0.1 g L
−1 of this bioproduct, which highlights its biostimulant potential. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
3. Chlorella vulgaris and Tetradesmus obliquus Protect Spinach (Spinacia oleracea L.) against Fusarium oxysporum.
- Author
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Viana, Catarina, Genevace, Méanne, Gama, Florinda, Coelho, Luísa, Pereira, Hugo, Varela, João, and Reis, Mário
- Subjects
SPINACH ,FUSARIUM oxysporum ,PHYTOPATHOGENIC fungi ,BIOLOGICAL pest control agents ,SCENEDESMUS obliquus ,FUNGAL growth ,CHLORELLA vulgaris ,DISEASE incidence - Abstract
Chlorella vulgaris and Tetradesmus obliquus were tested as biocontrol agents against the phytopathogenic fungus Fusarium oxysporum. This evaluation was conducted through in vitro and in vivo trials with spinach (Spinacia oleracea L.). The in vitro trials showed that C. vulgaris and T. obliquus were able to inhibit the phytopathogen, showing a similar inhibitory effect to that of the positive controls (Rovral, BASF
® and Biocontrol T34, Biocontrol Technologies® S.L.). C. vulgaris aqueous suspensions at 3.0 g L−1 led to a hyphal growth of 0.55 cm, each corresponding to a reduction of 63% of fungal growth. With T. obliquus, the hyphal growth was 0.53 cm when applied at a concentration of 0.75 g L−1 , having an inhibition of fungus growth of 64%. Thereafter, these results were validated in an in vivo trial on spinach using the same controls. The results revealed a lower severity and disease incidence and a reduction in the disease's AUDPC (area under the disease progress curve) when spinach was treated with the microalgae suspensions. Overall, these findings highlight the potential of C. vulgaris and T. obliquus suspensions as promising biocontrol agents against F. oxysporum in spinach when applied through irrigation. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
4. Brief characterisation of Fe chlorosis in chia ( Salvia hispanica L.) plants grown in nutrient solution
- Author
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Vieira, João, primary, Džidić-Uzelac, Luna, additional, Thais dos Santos, Izabela, additional, Patrício, Marco, additional, Gama, Florinda, additional, Saavedra, Teresa, additional, Correia, Pedro José, additional, and Pestana, Maribela, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Can Bicarbonate Enhance the Performance of Carob Seedlings Grown in Nutrient Solutions with Different Fe Concentrations?
- Author
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Gama, Florinda, Correia, Pedro José, Saavedra, Teresa, Dandlen, Susana, de Varennes, Amarilis, Nolasco, Gustavo, and Pestana, Maribela
- Published
- 2020
- Full Text
- View/download PDF
6. Nutritional Performance of Five Citrus Rootstocks under Different Fe Levels
- Author
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Pestana, Maribela, primary, García-Caparrós, Pedro, additional, Saavedra, Teresa, additional, Gama, Florinda, additional, Abadía, Javier, additional, Varennes, Amarilis de, additional, and Correia, Pedro José, additional
- Published
- 2023
- Full Text
- View/download PDF
7. Responses of tomato (Solanum lycopersicum L.) plants to iron deficiency in the root zone
- Author
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Jiménez María Rocío, Casanova Laura, Saavedra Teresa, Gama Florinda, Suárez María Paz, Correia Pedro José, and Pestana Maribela
- Subjects
chlorophyll ,ferric-chelate reductase (fcr) ,plant nutrition ,root lateralization ,stress physiology ,subapical root swelling ,Plant culture ,SB1-1110 - Abstract
Iron deficiency induces a yellowing in the aerial part of plants, known as iron chlorosis, and reduces the growth, yield, and quality of the fruits. Understanding plant response to iron deficiency is essential for agronomic management. This study decoded the temporal response of tomato plants (Solanum lycopersicum L.) to iron deficiency by quantifying different vegetative parameters. Subapical root swelling in the first 2.0 mm and several shoot and root growth parameters were measured in plants grown in a nutrient solution with and without Fe, on different dates designated as days after transplantation (DAT). Correlations between the total chlorophyll concentration in young leaves and 22 morphological and physiological parameters were also calculated. The plants grown in the absence of Fe had a higher number of secondary roots at 3 DAT, compared to control plants. On the same date, subapical root swelling was also observed, particularly at 1.5 and 2.0 mm from the root tip. Those plants also had a lower chlorophyll content in young leaves and a higher ferric-chelate reductase activity (FCR; EC 1.16.1.17) in the roots. At 9 DAT, the overall vegetative performance (plant height, fresh weight of stems and leaves) was negatively affected. At the end of the experiment (14 DAT), significant correlations were found between chlorophyll and the studied parameters. In conclusion, tomato plants experienced a cascade of responses to Fe deficiency throughout nine days: firstly, root lateralization increased; later, root swelling was observed, and a decrease in leaf chlorophyll content was registered associated with an increase in root FCR. At the end, the biomass of tomato plants decreased.
- Published
- 2019
- Full Text
- View/download PDF
8. Silencing of FRO1 gene affects iron homeostasis and nutrient balance in tomato plants
- Author
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Gama, Florinda, primary, Saavedra, Teresa, additional, Dandlen, Susana, additional, García‐Caparrós, Pedro, additional, de Varennes, Amarilis, additional, Nolasco, Gustavo, additional, Correia, Pedro José, additional, and Pestana, Maribela, additional
- Published
- 2023
- Full Text
- View/download PDF
9. Nutritional Performance of Five Citrus Rootstocks under Different Fe Levels
- Author
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Foundation for Science and Technology, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Pestana, Maribela, García-Caparrós, Pedro, Saavedra, Teresa, Gama, Florinda, Abadía Bayona, Javier, Varennes, Amarilis de, Correia, Pedro José, Foundation for Science and Technology, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Pestana, Maribela, García-Caparrós, Pedro, Saavedra, Teresa, Gama, Florinda, Abadía Bayona, Javier, Varennes, Amarilis de, and Correia, Pedro José
- Abstract
Iron is an essential micronutrient for citrus, playing an important role in photosynthesis and yield. The aim of this paper was to evaluate the tolerance to Fe deficiency of five citrus rootstocks: sour orange (S), Carrizo citrange (C), Citrus macrophylla (M), Troyer citrange (T), and Volkamer lemon (V). Plants were grown for 5 weeks in nutrient solution that contained the following Fe concentrations (in µM): 0, 5, 10, 15, and 20. At the end of the experiment, biomass (dry weight—DW), leaf area, total leaf chlorophyll (CHL), and the activity of root chelate reductase (FCR) were recorded. Additionally, the mineral composition of roots (R) and shoots (S) was evaluated. Principal component analysis was used to study the relationships between all parameters and, subsequently, the relations between rootstocks. In the first component, N-S, P-S, Ca-S, Cu-S, Zn-S, Mn-S, Zn-R, and Mn-R concentrations were related to leaf CHL and FCR. Increases in leaf CHL, Mg-R, and DW (shoots and roots) were inversely related to Cu-R, which was shown in the second component. The values obtained were consistent for V10, C15, and C20, but in contrast for S0 and S5. In conclusion, micronutrient homeostasis in roots and shoots of all rootstocks were affected by Fe stress conditions. The Fe/Cu ratio was significantly related to CHL, which may be used to assist rootstock performance.
- Published
- 2023
10. Correction to: Can Bicarbonate Enhance the Performance of Carob Seedlings Grown in Nutrient Solutions with Different Fe Concentrations?
- Author
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Gama, Florinda, Correia, Pedro José, Saavedra, Teresa, Dandlen, Susana, de Varennes, Amarilis, Nolasco, Gustavo, and Pestana, Maribela
- Published
- 2020
- Full Text
- View/download PDF
11. Effects of foliar application of organic acids on strawberry plants
- Author
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Saavedra, Teresa, primary, Gama, Florinda, additional, Rodrigues, Maria A., additional, Abadía, Javier, additional, de Varennes, Amarilis, additional, Pestana, Maribela, additional, Da Silva, José Paulo, additional, and Correia, Pedro José, additional
- Published
- 2022
- Full Text
- View/download PDF
12. Algae as Food in Europe: An Overview of Species Diversity and Their Application
- Author
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Mendes, Madalena, primary, Navalho, Sofia, additional, Ferreira, Alice, additional, Paulino, Cristina, additional, Figueiredo, Daniel, additional, Silva, Daniel, additional, Gao, Fengzheng, additional, Gama, Florinda, additional, Bombo, Gabriel, additional, Jacinto, Rita, additional, Aveiro, Susana, additional, Schulze, Peter, additional, Gonçalves, Ana Teresa, additional, Pereira, Hugo, additional, Gouveia, Luisa, additional, Patarra, Rita, additional, Abreu, Maria Helena, additional, Silva, Joana, additional, Navalho, João, additional, Varela, João, additional, and Speranza, Lais, additional
- Published
- 2022
- Full Text
- View/download PDF
13. Algae as Food in Europe : An Overview of Species Diversity and Their Application†
- Author
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Mendes, Madalena Caria, Navalho, Sofia, Ferreira, Alice, Paulino, Cristina, Figueiredo, Daniel, Silva, Daniel, Gao, Fengzheng, Gama, Florinda, Bombo, Gabriel, Jacinto, Rita, Aveiro, Susana S., Schulze, Peter S.C., Gonçalves, Ana Teresa, Pereira, Hugo, Gouveia, Luisa, Abreu, Maria Helena, Silva, Joana L., Navalho, João, Varela, João C.S., Speranza, Lais Galileu, Mendes, Madalena Caria, Navalho, Sofia, Ferreira, Alice, Paulino, Cristina, Figueiredo, Daniel, Silva, Daniel, Gao, Fengzheng, Gama, Florinda, Bombo, Gabriel, Jacinto, Rita, Aveiro, Susana S., Schulze, Peter S.C., Gonçalves, Ana Teresa, Pereira, Hugo, Gouveia, Luisa, Abreu, Maria Helena, Silva, Joana L., Navalho, João, Varela, João C.S., and Speranza, Lais Galileu
- Abstract
Algae have been consumed for millennia in several parts of the world as food, food supplements, and additives, due to their unique organoleptic properties and nutritional and health benefits. Algae are sustainable sources of proteins, minerals, and fiber, with well-balanced essential amino acids, pigments, and fatty acids, among other relevant metabolites for human nutrition. This review covers the historical consumption of algae in Europe, developments in the current European market, challenges when introducing new species to the market, bottlenecks in production technology, consumer acceptance, and legislation. The current algae species that are consumed and commercialized in Europe were investigated, according to their status under the European Union (EU) Novel Food legislation, along with the market perspectives in terms of the current research and development initiatives, while evaluating the interest and potential in the European market. The regular consumption of more than 150 algae species was identified, of which only 20% are approved under the EU Novel Food legislation, which demonstrates that the current legislation is not broad enough and requires an urgent update. Finally, the potential of the European algae market growth was indicated by the analysis of the trends in research, technological advances, and market initiatives to promote algae commercialization and consumption.
- Published
- 2022
14. Nannochloropsis Oceanica Biomass Enriched by Electrocoagulation Harvesting with Promising Agricultural Applications
- Author
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Figueiredo, Daniel, primary, Ferreira, Alice, additional, Gama, Florinda, additional, and Gouveia, Luisa, additional
- Published
- 2022
- Full Text
- View/download PDF
15. Nutritional and Functional Evaluation of Inula crithmoides and Mesembryanthemum nodiflorum Grown in Different Salinities for Human Consumption
- Author
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Lima, Alexandre R., primary, Gama, Florinda, additional, Castañeda-Loaiza, Viana, additional, Costa, Camila, additional, Schüler, Lisa M., additional, Santos, Tamára, additional, Salazar, Miguel, additional, Nunes, Carla, additional, Cruz, Rui M. S., additional, Varela, João, additional, and Barreira, Luísa, additional
- Published
- 2021
- Full Text
- View/download PDF
16. Influence of cultivation salinity in the nutritional composition, antioxidant capacity and microbial quality of Salicornia ramosissima commercially produced in soilless systems
- Author
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Lima, Alexandre R., primary, Castañeda-Loaiza, Viana, additional, Salazar, Miguel, additional, Nunes, Carla, additional, Quintas, Célia, additional, Gama, Florinda, additional, Pestana, Maribela, additional, Correia, Pedro J., additional, Santos, Tamára, additional, Varela, João, additional, and Barreira, Luísa, additional
- Published
- 2020
- Full Text
- View/download PDF
17. Wild vs cultivated halophytes: Nutritional and functional differences
- Author
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Castañeda-Loaiza, Viana, primary, Oliveira, Marta, additional, Santos, Tamára, additional, Schüler, Lisa, additional, Lima, Alexandre R., additional, Gama, Florinda, additional, Salazar, Miguel, additional, Neng, Nuno R., additional, Nogueira, J.M.F., additional, Varela, João, additional, and Barreira, Luísa, additional
- Published
- 2020
- Full Text
- View/download PDF
18. A novel plant extract as a biostimulant to recover strawberry plants from iron chlorosis
- Author
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Saavedra, Teresa, primary, Gama, Florinda, additional, Correia, Pedro José, additional, Da Silva, José Paulo, additional, Miguel, Maria Graça, additional, de Varennes, Amarilis, additional, and Pestana, Maribela, additional
- Published
- 2020
- Full Text
- View/download PDF
19. Correction to: Can Bicarbonate Enhance the Performance of Carob Seedlings Grown in Nutrient Solutions with Different Fe Concentrations?
- Author
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Gama, Florinda, primary, Correia, Pedro José, additional, Saavedra, Teresa, additional, Dandlen, Susana, additional, de Varennes, Amarilis, additional, Nolasco, Gustavo, additional, and Pestana, Maribela, additional
- Published
- 2019
- Full Text
- View/download PDF
20. Can Bicarbonate Enhance the Performance of Carob Seedlings Grown in Nutrient Solutions with Different Fe Concentrations?
- Author
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Gama, Florinda, primary, Correia, Pedro José, additional, Saavedra, Teresa, additional, Dandlen, Susana, additional, de Varennes, Amarilis, additional, Nolasco, Gustavo, additional, and Pestana, Maribela, additional
- Published
- 2019
- Full Text
- View/download PDF
21. Responses of tomato (Solanum lycopersicum L.) plants to iron deficiency in the root zone
- Author
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Universidad de Sevilla. Departamento de Ciencias Agroforestales, Jiménez González, María Rocío, Casanova Lerma, Laura, Saavedra, Teresa, Gama, Florinda, Suárez García, María Paz, Correia, Pedro José, Pestana, Maribela, Universidad de Sevilla. Departamento de Ciencias Agroforestales, Jiménez González, María Rocío, Casanova Lerma, Laura, Saavedra, Teresa, Gama, Florinda, Suárez García, María Paz, Correia, Pedro José, and Pestana, Maribela
- Abstract
Iron deficiency induces a yellowing in the aerial part of plants, known as iron chlorosis, and reduces the growth, yield, and quality of the fruits. Understanding plant response to iron deficiency is essential for agronomic management. This study decoded the temporal response of tomato plants (Solanum lycopersicum L.) to iron deficiency by quantifying different vegetative parameters. Subapical root swelling in the first 2.0 mm and several shoot and root growth parameters were measured in plants grown in a nutrient solution with and without Fe, on different dates designated as days after transplantation (DAT). Correlations between the total chlorophyll concentration in young leaves and 22 morphological and physiological parameters were also calculated. The plants grown in the absence of Fe had a higher number of secondary roots at 3 DAT, compared to control plants. On the same date, subapical root swelling was also observed, particularly at 1.5 and 2.0 mm from the root tip. Those plants also had a lower chlorophyll content in young leaves and a higher ferric-chelate reductase activity (FCR; EC 1.16.1.17) in the roots. At 9 DAT, the overall vegetative performance (plant height, fresh weight of stems and leaves) was negatively affected. At the end of the experiment (14 DAT), significant correlations were found between chlorophyll and the studied parameters. In conclusion, tomato plants experienced a cascade of responses to Fe deficiency throughout nine days: firstly, root lateralization increased; later, root swelling was observed, and a decrease in leaf chlorophyll content was registered associated with an increase in root FCR. At the end, the biomass of tomato plants decreased.
- Published
- 2019
22. Movimento e sinalização do ferro em plantas com estratégia I
- Author
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Gama, Florinda Maria Martins, Correia, Maribela Pestana, and Nolasco, Gustavo
- Subjects
Quelato de ferro redutase ,VIGS (Virus induced gene silencing) ,Ciências Naturais::Ciências da Terra e do Ambiente [Domínio/Área Científica] ,Deficiência de ferro ,Solo calcário ,Nutrientes - Abstract
A deficiência de Fe provocou decréscimos no teor de clorofila total, acréscimos na atividade da QFR, alterações morfológicas a nível radicular, diminuição de absorção e translocação de Fe nos diferentes órgãos. No morangueiro o decréscimo do Fe incidiu nas raízes, na coroa e nas flores. No Poncirus o teor de Fe diminuiu nas raízes. Como alternativa à absorção de Fe ocorreu uma acumulação dos metais Zn, Mn e Cu especialmente nas raízes e folhas novas. No morangueiro os compostos orgânicos acumularam nas folhas velhas e jovens e diminuíram nas raízes. O pulso de Fe fornecido na solução nutritiva a plantas cloróticas de morangueiro levou à recuperação da deficiência em 12 dias. A partição de Fe foi direcionada para a coroa e para as flores, associado a acumulação de ácidos sucínico e cítrico ao nível radicular e permitiu maior absorção de Fe. Foram estudadas as alterações no mecanismo de resposta em plantas com o gene FRO1 silenciado. Em plantas silenciadas a deficiência de Fe levou à redução da expressão do gene, decréscimo da atividade QFR e diminuição na síntese de clorofila. Em Nicotiana o padrão de distribuição do Fe entre folhas velhas e jovens foi alterado e não foi possível recuperar as plantas silenciadas, as plantas não conseguiram retomar o metabolismo na síntese de clorofila nem ativar a enzima QFR. Foi estudado o comportamento de porta-enxertos de Poncirus com a estratégia redutora e as adaptações fisiológica ativas quando transplantadas para solo calcário. Estas plantas apresentaram desempenho semelhante em relação às plantas que cresceram numa primeira fase sem limitação de Fe indicando que os mecanismos de adaptação e o aumento nutricional induzidos pela deficiência de Fe podem ser utilizados como uma ferramenta de defesa que contribuirá para as plantas tolerarem melhor as diferentes restrições do solo em condições de campo. Fe deficiency caused decreases in total chlorophyll, increases in ferric chelate reductase activity, morphological alterations at the root level and decreases in Fe absorption and translocation in different organs. In strawberry plants, Fe content decreased in the roots, the crown and the flowers. In Poncirus, the Fe content decreased in the roots. As an alternative to Fe absorption, an accumulation of Zn, Mn and Cu metals occurred especially in roots and young leaves. In strawberry organic compounds accumulated in mature and young leaves and lowered in the roots. The Fe pulse added to the nutrient solution of chlorotic strawberry plants led to Fe deficiency recovery after 12 days. Fe partitioning was directed to the crown and flowers, associated with the accumulation of succinic and citric acids at the root level, which allowed higher Fe absorption. Changes to the response mechanism in plants with the FRO1 gene silenced were studied. In silenced plants, Fe deficiency led to reduced gene expression, decreased FCR activity and decreased chlorophyll synthesis. In Nicotiana, the pattern of Fe distribution between mature and young leaves was altered and silenced plants were unable to recover after Fe resupply, the plants were unable to resume chlorophyll synthesis nor activate the ferric chelate reductase enzyme. The behaviour of Poncirus rootstocks with the reduction-based strategy induced was analysed when transplanted to calcareous soil. These plants presented similar performance in regard to plants that grew in a first stage with Fe sufficient conditions indicating that physiological and morphological adaptations and the nutritional increase induced by Fe deficiency can be used as a defence tool that may contribute to more tolerant plants to different soil environments under field conditions.
- Published
- 2017
23. Changes in nutritional homeostasis ofPoncirus trifoliataandCeratonia siliquaas a response to different iron levels in nutrient solution
- Author
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José Correia, Pedro, primary, de Varennes, Amarilis, additional, Gama, Florinda, additional, Saavedra, Teresa, additional, and Pestana, Maribela, additional
- Published
- 2018
- Full Text
- View/download PDF
24. Biologically active compounds available in Ceratonia siliqua L. grown in contrasting soils under Mediterranean climate
- Author
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Correia, Pedro José, primary, Saavedra, Teresa, additional, Gama, Florinda, additional, da Graça Miguel, Maria, additional, de Varennes, Amarilis, additional, and Pestana, Maribela, additional
- Published
- 2018
- Full Text
- View/download PDF
25. Silencing of the FRO1 gene and its effects on iron partition in Nicotiana benthamiana
- Author
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Gama, Florinda, primary, Saavedra, Teresa, additional, Dandlen, Susana, additional, de Varennes, Amarilis, additional, Correia, Pedro J., additional, Pestana, Maribela, additional, and Nolasco, Gustavo, additional
- Published
- 2017
- Full Text
- View/download PDF
26. Partição da biomassa, composição mineral e variação de ácidos orgânicos em morangueiros deficientes em ferro
- Author
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Saavedra, Teresa, Gama, Florinda, Silva, José Paulo da, Miguel, Maria da Graça, de Varennes, Amarilis, Abadía Bayona, Anunciación, Correia, Pedro José, and Pestana, Maribela
- Subjects
education - Published
- 2014
27. Correction to: Can Bicarbonate Enhance the Performance of Carob Seedlings Grown in Nutrient Solutions with Different Fe Concentrations?
- Author
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Gama, Florinda, Correia, Pedro José, Saavedra, Teresa, Dandlen, Susana, de Varennes, Amarilis, Nolasco, Gustavo, and Pestana, Maribela
- Abstract
Readers should note that there was an error in this article as originally published.
- Published
- 2024
- Full Text
- View/download PDF
28. Iron mobility in chlorotic leaves of strawberry plants grown in nutrient solution
- Author
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Gama, Florinda, Correia, Pedro José, Silva, José Paulo da, Saavedra, Teresa, El-Jendoubi, Hamdi, Abadía Bayona, Anunciación, de Varennes, Amarilis, and Pestana, Maribela
- Subjects
education - Published
- 2014
29. Organic acids and nutrient profile in response to iron deficiency and resupply
- Author
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Gama, Florinda, Saavedra, Teresa, Silva, José Paulo da, Miguel, Maria da Graça, de Varennes, Amarilis, Abadía Bayona, Anunciación, Correia, Pedro José, and Pestana, Maribela
- Subjects
education - Published
- 2014
30. The memory of iron stress in strawberry plants
- Author
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Gama, Florinda, primary, Saavedra, Teresa, additional, da Silva, José Paulo, additional, Miguel, Maria Graça, additional, de Varennes, Amarilis, additional, Correia, Pedro José, additional, and Pestana, Maribela, additional
- Published
- 2016
- Full Text
- View/download PDF
31. Changes in nutritional homeostasis of Poncirus trifoliata and Ceratonia siliqua as a response to different iron levels in nutrient solution.
- Author
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José Correia, Pedro, de Varennes, Amarilis, Gama, Florinda, Saavedra, Teresa, and Pestana, Maribela
- Subjects
IRON deficiency diseases ,CAROB ,CITRUS ,CHLOROSIS (Plants) ,TRACE elements in plant nutrition ,IRON chelates ,PLANT assimilation ,PLANTS - Abstract
Iron (Fe) deficiency is a nutritional disorder in plants. Poncirus trifoliata is susceptible to Fe deficiency, but symptoms of Fe deficiency are rare in Ceratonia siliqua, a slow-growing species. Specimens of the two species were grown in nutrient solutions containing three Fe concentrations: without Fe (0 µM), 1 µM Fe, and either 10 µM Fe (for Ceratonia) or 40 µM Fe (for P. trifoliata). Growth, the degree of chlorosis, the plant mineral composition, and the activity of the root ferric chelate-reductase (FCR) were assessed. Ceratonia plants exposed to 1 µM Fe were efficient at using Fe in the synthesis of chlorophyll. The activity of FCR was enhanced in the total absence of Fe. In Poncirus a low activity of the FCR was observed in plants with no Fe. The balance between micronutrients in the Ceratonia roots was not affected with 1 µM Fe compared with the higher Fe concentration treatments. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
32. A caracterização e correção da deficiência de ferro em plantas de morangueiro: novas abordagens
- Author
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Pestana, Maribela, Gama, Florinda, Saavedra, Teresa, Pinto, João Castro, Abadia, Anunciación, Varennes, A., and Correia, Pedro José
- Subjects
SPAD ,food and beverages ,iron chlorosis ,strawberry ,ferric chelate redutase - Abstract
Iron (Fe) is abundant in soils and although it is required in small amounts by plants the incidence of iron chlorosis (Fe deficiency) is very common in a number of crops and requires massive soil application of Fe-chelates to correct it. In this work, we present the most important results obtained in several experiments conducted with strawberry to study the physiological and biochemical response mechanisms to Fe deficiency, and the assessment of novel alternatives to control this nutritional disorder. In all experiments, conducted in hydroponic systems, symptoms were induced by withdrawing Fe from the solution and the results were compared to a control treatment grown with Fe. The degree of chlorosis and symptoms recovery was estimated using SPAD values. The activity of iron chelate reductase, the enzyme responsible for Fe reduction in roots, was determined in root apices by colorimetric quantification of the BPDS complex. The Fe concentration in leaves and roots was quantified by atomic absorption spectrophotometry after treatments at 450 ºC and acid digestion of the ashes obtained. Strawberry plants that grew always without Fe, presented Fe chlorosis and morphological external root modifications associated with increases of the activity of the Fe-reductase enzyme. The recovery of chlorotic plants was achieved by application of Fe sulphate either to leaves or to the nutrient solution. In plants recovered by using Fe in the solution, the enzyme maintained a large activity, suggesting a strategy to increase plant Fe pools. As an alternative to synthetic Fe chelates, we also tested a foliar application of a plant extract obtained from fresh grass clippings (national patent PT/103584-2009 of UALG, and international patent PCT/PT2007/000041-2008, UALG and ADPFertilizantes), which was effective in chlorosis recovery after three applications. The results are discussed in order to highlight the practical implications of these responses under a perspective of optimization of crop Fe fertilization
- Published
- 2012
33. Aplicação da deteção remota à avaliação nutricional de fruteiras
- Author
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Gama, Florinda, Luis, Joaquim, and Correia, Maribela Pestana
- Subjects
Deteção remota ,Clonose férrica ,Citrinos ,Índice de vegetação - Abstract
Dissertação de mest., Geomática (Análise de Sistemas Ambientais), Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2012 Devido à necessidade de encontrar técnicas mais rápidas e eficazes de diagnóstico de deficiências nutricionais em campo, torna-se importante explorar a potencialidade do uso de imagens de satélite com método de monitorização da clorose férrica em citrinos na região do Algarve, através do processamento de imagens determinando os índices de vegetação mais adequados ao diagnóstico e se possível ainda antes do aparecimento de sintomas para que as correções desta deficiência sejam feitas atempadamente. Para tal selecionaram-se dois pomares de citrinos da cultivar Newhall e utilizou-se um total de 152 árvores para a calibração do modelo e um terceiro pomar para a validação do modelo da cultivar Valencia late, localizados no município de Tavira, Portugal. O modelo foi elaborado utilizando medições de clorofila pelo método não destrutivo através da utilização do aparelho SPAD-502 e posterior conversão destes valores em clorofila total através de uma curva de calibração. Colheram-se entre 20 a 30 folhas sãs por árvore e realizaram-se as leituras de clorofila numa amostragem de 15 folhas por árvore. Adquiriu-se uma imagem de satélite/sensor multiespectral RapidEye com uma área de 625 km2, uma resolução espacial de 5 m de pixel e uma resolução espectral de 5 bandas (em nm): 440-510, 520-590, 630-685, 690-730 e 760-850 respetivamente para: azul, verde, vermelho, red edge e infravermelho próximo. De modo a avaliar a potencialidade na utilização desta imagem para um diagnóstico precoce de clorose férrica foram analisados índices de vegetação como: o NDVI, o NDVIr, o SPADI, o SPADIr, o MCARI, o TCARI e o OSAVI, determinados por combinações aritméticas de duas ou mais bandas. Verificou-se que o índice SPADIr foi o modelo mais apropriado na estimação dos conteúdos em clorofila total nas folhas de laranjeira desta cultivar e que possui capacidade de diagnosticar cloroses em campo que foi comprovado através da validação.
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- 2012
34. Fe deficiency induction in Poncirus trifoliata rootstock growing in nutrient solution changes its performance after transplant to soil
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Gama, Florinda, primary, Saavedra, Teresa, additional, Díaz, Isabel, additional, Campillo, María del Carmen, additional, de Varennes, Amarilis, additional, Duarte, Amílcar, additional, Pestana, Maribela, additional, and Correia, Pedro José, additional
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- 2015
- Full Text
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35. Evaluation of Fe deficiency effects on strawberry fruit quality
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Gama, Florinda, Saavedra, T., Miguel, Maria Graça, and Dandlen, S. A.
- Abstract
The effects of Fe deficiency on the antioxidant properties of strawberry juice was carried out with a day-neutral cultivar 'Selva'. Bare root transplants (without leaves) with approximately 18 cm, were transferred to Hoagland's nutrient solution with (Fe2.5) and without Fe (Fe0), using Fe-EDDHMA as the Fe source: 0 and 2.5 5 μM Fe. Plants were grown in 20 L containers in a glasshouse for 6 weeks (from April 27 to June 5) under natural light and air temperature ≤ 25°C. Twelve transplants were used per treatment, distributed in a complete randomized design. Plants grown in absence of Fe revealed chlorotic symptoms approximately after three weeks, based on SPAD values measured in young leaves (
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- 2010
36. Changes in the concentration of organic acids in roots and leaves of carob-tree under Fe depletion
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Correia, Pedro José, Gama, Florinda, Saavedra, Teresa, Miguel, Maria da Graça, Silva, José Paulo da, Abadía Bayona, Anunciación, Varennes, Amarilis de, Pestana, Maribela, Correia, Pedro José, Gama, Florinda, Saavedra, Teresa, Miguel, Maria da Graça, Silva, José Paulo da, Abadía Bayona, Anunciación, Varennes, Amarilis de, and Pestana, Maribela
- Abstract
Several fruit trees are able to cope with iron (Fe) deficiency when grown in calcareous soils in the Mediterranean region, although information regarding well adapted slow-growing species is scarce, and the mechanisms activated by these species are not described in the literature. A crucial issue related to tolerance is the need to transport Fe over relatively long distances inside the plant. To evaluate the possible role of organic acids in the movement of Fe in tolerant plants, we studied the concentration of low molecular weight organic acids in several organs of 1-year old carob plants grown for 55 days in nutrient solutions without Fe (0 µM Fe) or with 1 µM Fe and 10 µM Fe. Roots, stems and leaves were harvested, and the biomass, Fe and organic acid contents quantified. Total leaf chlorophyll (Chl) was evaluated in young leaves over the experimental period and the activity of root ferric chelate-reductase (FC-R; EC 1.16.1.17) was determined after 35 days, when deficiency symptoms appeared. Iron chlorosis was observed only at the end of the experiment in plants grown in the absence of Fe, and these plants had a smaller DW of leaves and also significant greater activity of root FC-R. Iron deficiency (Fe0 and Fe1 treatments) induced significant changes in the concentrations of succinic, malic, citric and fumaric acids, which increased in roots, or in basal, middle and apical leaves. There were significant correlations between most organic acids (with the exceptions of 2-oxoglutaric and tartaric acids) and leaf Chl. Analysis of each type of leaf showed that more succinic and malic acids were present in young chlorotic leaves while the reverse was true for quinic acid. These changes in organic acids followed a root-to-foliage pathway that was similar in all leaf types and particularly evident in young chlorotic leaves. We hypothesised that it was associated with Fe transport from roots to aboveground tissues, as there were significant differences in Fe contents betw
- Published
- 2014
37. Changes in the concentration of organic acids in roots and leaves of carob-tree under Fe deficiency
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Correia, Pedro José, primary, Gama, Florinda, additional, Saavedra, Teresa, additional, Miguel, Maria Graça, additional, Paulo Da Silva, José, additional, Abadía, Anunciación, additional, de Varennes, Amarilis, additional, and Pestana, Maribela, additional
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- 2014
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38. ROOT FERRIC CHELATE REDUCTASE IS REGULATED BY IRON AND COPPER IN STRAWBERRY PLANTS
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Pestana, Maribela, primary, Correia, Pedro José, additional, Saavedra, Teresa, additional, Gama, Florinda, additional, Dandlen, Susana, additional, Nolasco, Gustavo, additional, and Varennes, Amarilis de, additional
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- 2013
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39. Development and recovery of iron deficiency by iron resupply to roots or leaves of strawberry plants
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Pestana, Maribela, primary, Correia, Pedro José, additional, Saavedra, Teresa, additional, Gama, Florinda, additional, Abadía, Anunciación, additional, and de Varennes, Amarilis, additional
- Published
- 2012
- Full Text
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40. Strawberry recovers from iron chlorosis after foliar application of a grass‐clipping extract
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Pestana, Maribela, primary, Domingos, Irina, additional, Gama, Florinda, additional, Dandlen, Susana, additional, Miguel, Maria Graça, additional, Castro Pinto, João, additional, de Varennes, Amarilis, additional, and Correia, Pedro José, additional
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- 2011
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41. Brief characterisation of Fe chlorosis in chia (<italic>Salvia hispanica</italic> L.) plants grown in nutrient solution.
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Vieira, João, Džidić-Uzelac, Luna, Thais dos Santos, Izabela, Patrício, Marco, Gama, Florinda, Saavedra, Teresa, Correia, Pedro José, and Pestana, Maribela
- Abstract
Chia (
Salvia hispanica L.) plant is a well-known plant due to the nutraceutical value of its seeds. The aim of this preliminary study was to assess the response of Chia plants to Fe deficiency. Chia plants were grown for 12 days in Hoagland’s nutrient solutions without Fe (Fe0) and with Fe (Fe10-10 µM Fe). Biomass parameters and root ferric chelate-reductase activity (FC-R; EC 1.16.1.17) were determined at the end of the experiment. Chlorophyll estimations (expressed as SPAD readings) decreased progressively, showing the typical symptoms of iron chlorosis. In addition, iron-deficient chia plants exhibit smaller biomass (number of leaves, root, and shoot growth reduction) compared to control plants. These plants also showed morphological changes in roots. Furthermore, root FC-R activity was significantly lower in Fe0 plants. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
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42. Study of the biopesticide and biostimulant activity of microalgae in horticultural species
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Viana, Catarina, Reis, Mário, and Gama, Florinda Maria Martins
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Bioestimulantes ,Microalgas ,Agricultura sustentável ,Biopesticidas ,Ciências Sociais::Outras Ciências Sociais [Domínio/Área Científica] ,Agentes fitopatogénicos ,Fitofármacos - Abstract
As algas microscópicas e as cianobactérias fazem parte de um diverso grupo de organismos unicelulares designados por microalgas. Este grupo é composto por organismos fotossintéticos, responsáveis por 60 % da produção da primária da Terra e podem ser encontrados em diferentes habitats, na sua maioria aquáticos. Devido à sua capacidade de captação de CO2 atmosférico, à disponibilidade de água e a luz solar, produzem grandes quantidades de biomassa e com isso apresentam uma maior atividade fotossintética que as plantas. O seu crescimento heterotrófico, a sua natureza versátil e o seu rápido desenvolvimento, têm vindo a chamar à atenção e a ser utilizados em indústrias diversificadas, como a farmacêutica, cosmética, dietética, biocombustíveis, de entre outras. Para além destas aplicações , as microalgas estão a ser alvo de estudos tendo em conta a sua relação com o processo de mineralização, na circulação de matéria orgânica e inorgânica, a sua rica composição em macro- e micronutrientes e a sua capacidade de produção de compostos bioativos, como polissacarídeos, fitohormonas e até mesmo compostos fenólicos, o que os levou a serem vistos como possíveis candidatos a substitutos dos produtos químicos e sintéticos, nomeadamente dos pesticidas e fertilizantes. A crescente preocupação com a necessidade de aumentar tanto a produtividade como a qualidade e combater as doenças que afetam a culturas, fez com que os produtores recorressem a produtos que lhes garantam um maior crescimento das suas culturas, nomeadamente os fertilizantes e os pesticidas. Como consequência, houve um uso abusivo de sustâncias que, em grande quantidade, são prejudiciais tanto para o ambiente como para a saúde humana. Essas consequências podem ser evitadas se se recorrer ao uso de fertilizantes orgânicos, sendo também referenciados como excelentes alternativas para a reciclagem de nutrientes e o aumento de carbono (C) no solo e mitigando as alterações climáticas. Para além destas vantagens, os compostos produzidos pelas microalgas também contribuem para um solo saudável e por acréscimo, uma microbiota benéfica. O agravamento do efeito estufa na Terra e as alterações climáticas provocadas, levou a União Europeia a formular um acordo em 2019 (European Green Deal), com o principal objetivo de tornar a Europa um continente com um impacto ambiental neutro até 2050. Para tal, é fundamental reduzir a emissão de gases em 55% até 2030 em comparação com 1990. Paralelamente, a aplicação da Diretiva n.º 2009/128/CE, do Parlamento Europeu e do Conselho, de 21 de outubro, através da Lei n.º 26/2013 de 11 de abril, reduziu significativamente a quantidade de substâncias ativas homologadas. Estas restrições abriram portas para a introdução de uma agricultura mais sustentável. Bioestimulantes e biopesticidas têm sido estudados como possíveis alternativas aos fertilizantes sintéticos e pesticidas, devido à sua já reportada eficácia. Estes compostos biológicos são seguros para o ambiente e ricos em nutrientes. A biomassa das microalgas contém inúmeros aminoácidos promotores do crescimento das plantas, bem como fitohormonas que aumentam a produção, estimulando o desenvolvimento das raízes e dos ramos. Para além disso, já foi reportado que o uso da biomassa destes microrganismos reduziu o desenvolvimento de diversos fungos fitopatogénicos. Este trabalho visou o estudo do potencial biopesticida das microalgas Scenedesmus obliquus e Chlorella vulgaris, através da avaliação in vitro e in vivo da sua ação inibitória de fungos fitopatogénicos e do seu efeito no desenvolvimento de plantas hortícolas. Para avaliar o seu efeito inibidor do crescimento de fungos e oomicetas, foram preparadas suspensões aquosas com biomassa das duas microalgas em diferentes concentrações, que numa primeira fase foram testadas in vitro contra Fusarium oxysporum, Botrytis cinerea, Alternaria alternata, Sclerotium rolfsii, Clarireedia spp., Colletotrichum gloeosporioides e Phytophthora cinnamomi. Estes agentes fitopatogénicos são os responsáveis pelo aparecimento de doenças nas plantas cultivadas, ameaçando a produção de alimentos e a segurança alimentar das populações. Numa segunda fase, foram conduzidos testes in vivo, onde se inocularam substratos hortícolas em vaso com micélios ativos de Fusarium oxysporum e Sclerotium rolfsii onde se plantou espinafre e alface, respetivamente. Para além destes fungos, outras plantas de espinafre e alface foram pulverizadas com uma suspensão de Botrytis cinerea. Este modo de aplicação das suspensões foi escolhido tendo em conta o tipo de fungo: no caso de F. oxysporum e S. rolfsii que são fungos do solo, o modo de aplicação das suspensões de microalgas foi por rega; no caso de B. cinerea, devido a ser um fungo da parte aérea da planta, o modo de aplicação selecionado foi a pulverização direta na parte aérea da planta. Estas aplicações decorreram semanalmente, durante o período de cultivo, de modo a avaliar o desenvolvimento da doença quando em contacto com as suspensões. Neste trabalho, avaliou-se também o potencial bioestimulante das suspensões de microalgas em ensaios in vitro e in vivo, verificando in vitro, o efeito na germinação e no desenvolvimento da radícula, e in vivo avaliando o desenvolvimento da planta, através de medições biométricas no final dos ensaios. Apesar de já existirem diversos estudos sobre o efeito estimulante das microalgas, os estudos sobre o seu efeito inibitório continuam a ser escassos. Quando em contacto com as suspensões, foi identificada a supressão do crescimento dos fungos F. oxysporum, S. rolfsii, B. cinerea e C. gloeosporioides e do oomiceto P. cinnamomi, indicando uma aplicação promissora das microalgas. Nos ensaios in vivo, foi comprovado que as microalgas afetam o desenvolvimento das doenças nas plantas, mostrando resultados promissores quanto à aplicação das microalgas tanto para a sua proteção como para o seu crescimento. Assim sendo, o uso de microalgas eucarióticas revelou-se promissor, possibilitando a redução do uso de fungicidas sintéticos, limitando o impacto ecológico da agricultura. A sua composição demonstrou ser uma mais-valia para o desenvolvimento das plantas quando aplicadas nos testes do potencial bioestimulantes. O seu conteúdo em fitohormonas e aminoácidos são uma fonte nutritiva para as plantas promovendo o seu crescimento, sendo de esperar que melhorem o desenvolvimento das plantas e enriqueçam o meio onde são aplicadas. Microscopic algae and cyanobacteria are part of a diverse group composed of single-celled organisms called microalgae. This group is composed of photosynthetic organisms, responsible for 60% of the earth's primary production, and can be found in different habitats, mostly aquatic. Due to their atmospheric CO2 capture capacity, water availability and sunlight, they are producers of large amounts of biomass and thus present higher photosynthetic activity than plants. Their rich composition in macro- and micronutrients and their ability to produce bioactive compounds such as polysaccharides, phytohormones and even phenolic compounds, led them to be seen as possible candidates for substitutes for chemicals and synthetics, namely pesticides and fertilizers. The growing concern about the need to increase both productivity and good quality and combat diseases that will impact crops has caused producers to use compounds that would ensure accelerated growth of their crops, fertilizers and pesticides. Therefore, there has been an abusive use of substances that in large numbers are harmful to both the environment and human health. This work aims to study the biopesticide potential of the microalgae Scenedesmus obliquus and Chlorella vulgaris, through in vitro and in vivo evaluation, the inhibiting action of phytopathogenic fungi and their effect on the development of vegetables. The inhibiting effects of these microalgae were tested on phytopathogenic fungi: Fusarium oxysporum, Botrytis cinerea, Alternaria alternata, Sclerotium rolfsii, Clarireedia spp., Colletotrichum gloeosporioides and oomycete: Phytophthora cinnamomi. These agents are responsible for numerous diseases in agriculture, causing several losses. This work describes the promising inhibiting effect of suspensions on the development of Fusarium oxysporum, Botrytis cinerea, Sclerotium rolfsii, Colletotrichum gloeosporioides and Phytophthora cinnamomi. The same effect was observed during the in vivo trials, when applied microalgae suspensions in plants inoculated with F. oxysporum, S. rolfsii and B. cinerea. And the biostimulant effect on agricultural plants was also shown in this study.
- Published
- 2022
43. Estudo da salinidade no desenvolvimento de duas espécies halófitas (Suaeda maritima e Inula crithmoides)
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Ricardo, Ana Patricia Caetanito, Correia, P. J., and Gama, Florinda
- Subjects
Inula crithmoides ,Halófitas ,Produção intensiva ,Suaeda maritima ,Salinidade ,Ciências Naturais::Outras Ciências Naturais [Domínio/Área Científica] - Abstract
Este trabalho teve como objetivo testar diferentes níveis de salinidade no desenvolvimento de duas espécies de plantas halófitas (Suaeda maritima e Inula crithmoides), de forma a identificar qual o valor de salinidade da solução nutritiva onde estas espécies apresentam um melhor comportamento agronómico. Visto que a informação sobre as técnicas de produção intensiva, destas espécies, em sistemas comerciais é muito escassa, é importante estudar as potencialidades e otimização de fatores de produção em ambientes controlados. Suaeda maritima e Inula crithmoides são plantas halófitas dicotiledóneas, suculentas, que crescem naturalmente em zonas costeiras sob influência da água do mar, incluindo nos sapais da Ria Formosa. Ao longo do ensaio as plantas foram sujeitas a seis níveis de salinidade, nomeadamente (em dS m-1): 5, 13, 21, 29, 37 e 45. Estes valores foram estabelecidos incorporando cloreto de sódio (NaCl) na solução nutritiva. No sentido de se avaliar a tolerância das plantas ao “stress” de salinidade, foram feitas análises de vários parâmetros, tais como: a produtividade total e comercial, qualidade das plantas, biomassa e percentagem de humidade das amostras comerciais. Adicionalmente foi também analisado a qualidade nutricional, onde se identificou a composição mineral e se quantificaram vitaminas presentes da espécie Inula crithmoides. Os níveis de salinidade que apresentaram melhores resultados, ou seja, maior produtividade, sem alteração das suas qualidades físicas, químicas e nutricionais, para cada espécie estudada, foram 5 dSm-1 para Inula crithmoides, e 13 dSm-1 para Suaeda maritima. The aim of this work was to test different salinity levels in the development of two halophyte plant species (Suaeda maritima and Inula crithmoides), in order to identify the salinity value of the nutrient solution where these species show the best agronomic behavior. The information about intensive production techniques of these species in commercial systems is very scarce, so it is important to study the potential and optimization of the production factors in controlled environments. Suaeda maritima and Inula crithmoides are succulent dicotyledonous halophyte plants that grow naturally in coastal areas under the influence of seawater, including in the Ria Formosa marshes. During the experiment, the plants were subjected to six levels of salinity at (dS m-1): 5, 13, 21, 29, 37 and 45. These values were established by the incorporation of sodium chloride (NaCl) in the nutrient solution. To evaluate plant tolerance to salinity “stress”, several parameters were analyzed, such as total and marketable productivity, plant quality, biomass and moisture content of the marketable samples. Additionally, the nutritional quality was also analyzed, such as the mineral composition and the quantification of some vitamins present in Inula crithmoides species. The salinity levels that presented the best results, that is, the highest productivities, without alteration of their physical, chemical and nutritional qualities, for each studied species, were 5 dSm-1 for Inula crithmoides, and 13 dSm-1 for Suaeda maritima.
- Published
- 2019
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