Curto Sánchez, Elena, Mateus-Medina, Éder Fredy, Crespo Lessmann, Astrid, Osuna-Gómez, Rubén, Ujaldón-Miró, Cristina, García-Moral, Alba, Galván-Blasco, Paula, Soto-Retes, Lorena, Ramos-Barbón, David, Plaza, Vicente, Universitat Autònoma de Barcelona, Institut Català de la Salut, [Curto E, Mateus-Medina ÉF, Crespo-Lessmann A] Asthma Unit, Respiratory and Allergy Department, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Department of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain. [Osuna-Gómez R] Inflammatory Diseases Unit, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [Ujaldón-Miró C] Cellular Immunotherapy and Gene Therapy Group (GITG), Oncology, Hematology and Transplantation Laboratory, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [García-Moral A] Servei d’Al·lèrgia Pediàtrica, Unitat de Pneumologia Pediàtrica i Fibrosi Quística, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Galván-Blasco P] Servei d’Al·lergologia, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
Allergic asthma; Eosinophil subsets; Induced sputum Asma alérgica; Subconjuntos de eosinófilos; Esputo inducido Asma al·lèrgica; Subconjunts d'eosinòfils; Esput induït Two subsets of eosinophils have been described: resident eosinophils with homeostatic functions (rEOS) in healthy subjects and in patients with nonallergic eosinophilic asthma, and inflammatory eosinophils (iEOS) in blood and lung samples from patients with allergic asthma. We explored if it would be possible to identify different subsets of eosinophils using flow cytometry and the gating strategy applied to induced sputum. We conducted an observational cross-sectional single-center study of 62 patients with persistent allergic asthma. Inflammatory cells from induced sputum samples were counted by light microscopy and flow cytometry, and cytokine levels in the supernatant were determined. Two subsets of eosinophils were defined that we call E1 (CD66b-high and CD15-high) and E2 (CD66b-low and CD15-low). Of the 62 patients, 24 were eosinophilic, 18 mixed, 10 paucigranulocytic, and 10 neutrophilic. E1 predominated over E2 in the eosinophilic and mixed patients (20.86% vs. 6.27% and 14.42% vs. 4.31%, respectively), while E1 and E2 were similar for neutrophilic and paucigranulocytic patients. E1 correlated with IL-5, fractional exhaled nitric oxide, and blood eosinophils. While eosinophil subsets have been identified for asthma in blood, we have shown that they can also be identified in induced sputum. This research was supported by the Spanish Allergy and Clinical Immunology Society (SEAIC) by means of a grant awarded in the call of 2017 (reference 17/06) and a BRN—Fundació Pla i Armengol grant in the call of 2018. The funds provided contributed to the acquisition of the material necessary to carry out the study, but the collaborating entities had no role in the analysis or interpretation of the results.