Search

Your search keyword '"Gallego-Ortega, David"' showing total 287 results
Start Over Author "Gallego-Ortega, David"
287 results on '"Gallego-Ortega, David"'

2. Decorin, an exercise-induced secretory protein, is associated with improved prognosis in breast cancer patients but does not mediate anti-tumorigenic tissue crosstalk in mice

Author

Hjorth, Marit, Egan, Casey L., Telles, Guilherme D., Pal, Martin, Gallego-Ortega, David, Fuller, Oliver K., McLennan, Emma D., Gillis, Ryan D., Oh, Tae Gyu, Muscat, George E.O., Tegegne, Surafel, Mah, Michael SM, Skhinas, Joanna, Estevez, Emma, Adams, Timothy E., McKay, Matthew J., Molloy, Mark, Watt, Kevin I., Qian, Hongwei, Gregorevic, Paul, Cox, Thomas R., Hojman, Pernille, Midtgaard, Julie, Christensen, Jesper F., Friedrichsen, Martin, Iozzo, Renato V., Sloan, Erica K., Drew, Brian G., Wojtaszewski, Jørgen F.P., Whitham, Martin, and Febbraio, Mark A.

Published
2024
Full Text
View/download PDF

3. Systemic alterations in neutrophils and their precursors in early-stage chronic obstructive pulmonary disease

Author

Kapellos, Theodore S., Baßler, Kevin, Fujii, Wataru, Nalkurthi, Christina, Schaar, Anna C., Bonaguro, Lorenzo, Pecht, Tal, Galvao, Izabela, Agrawal, Shobhit, Saglam, Adem, Dudkin, Erica, Frishberg, Amit, de Domenico, Elena, Horne, Arik, Donovan, Chantal, Kim, Richard Y., Gallego-Ortega, David, Gillett, Tessa E., Ansari, Meshal, Schulte-Schrepping, Jonas, Offermann, Nina, Antignano, Ignazio, Sivri, Burcu, Lu, Wenying, Eapen, Mathew S., van Uelft, Martina, Osei-Sarpong, Collins, van den Berge, Maarten, Donker, Hylke C., Groen, Harry J.M., Sohal, Sukhwinder S., Klein, Johanna, Schreiber, Tina, Feißt, Andreas, Yildirim, Ali Önder, Schiller, Herbert B., Nawijn, Martijn C., Becker, Matthias, Händler, Kristian, Beyer, Marc, Capasso, Melania, Ulas, Thomas, Hasenauer, Jan, Pizarro, Carmen, Theis, Fabian J., Hansbro, Philip M., Skowasch, Dirk, and Schultze, Joachim L.

Published
2023
Full Text
View/download PDF

5. Temporal profiling of the breast tumour microenvironment reveals collagen XII as a driver of metastasis

Author

Papanicolaou, Michael, Parker, Amelia L., Yam, Michelle, Filipe, Elysse C., Wu, Sunny Z., Chitty, Jessica L., Wyllie, Kaitlin, Tran, Emmi, Mok, Ellie, Nadalini, Audrey, Skhinas, Joanna N., Lucas, Morghan C., Herrmann, David, Nobis, Max, Pereira, Brooke A., Law, Andrew M. K., Castillo, Lesley, Murphy, Kendelle J., Zaratzian, Anaiis, Hastings, Jordan F., Croucher, David R., Lim, Elgene, Oliver, Brian G., Mora, Fatima Valdes, Parker, Benjamin L., Gallego-Ortega, David, Swarbrick, Alexander, O’Toole, Sandra, Timpson, Paul, and Cox, Thomas R.

Published
2022
Full Text
View/download PDF

7. Homeostatic IL-13 in healthy skin directs dendritic cell differentiation to promote TH2 and inhibit TH17 cell polarization

Author

Mayer, Johannes U., Hilligan, Kerry L., Chandler, Jodie S., Eccles, David A., Old, Samuel I., Domingues, Rita G., Yang, Jianping, Webb, Greta R., Munoz-Erazo, Luis, Hyde, Evelyn J., Wakelin, Kirsty A., Tang, Shiau-Choot, Chappell, Sally C., von Daake, Sventja, Brombacher, Frank, Mackay, Charles R., Sher, Alan, Tussiwand, Roxane, Connor, Lisa M., Gallego-Ortega, David, Jankovic, Dragana, Le Gros, Graham, Hepworth, Matthew R., Lamiable, Olivier, and Ronchese, Franca

Published
2021
Full Text
View/download PDF

8. Author Correction: Homeostatic IL-13 in healthy skin directs dendritic cell differentiation to promote TH2 and inhibit TH17 cell polarization

Author

Mayer, Johannes U., Hilligan, Kerry L., Chandler, Jodie S., Eccles, David A., Old, Samuel I., Domingues, Rita G., Yang, Jianping, Webb, Greta R., Munoz-Erazo, Luis, Hyde, Evelyn J., Wakelin, Kirsty A., Tang, Shiau-Choot, Chappell, Sally C., von Daake, Sventja, Brombacher, Frank, Mackay, Charles R., Sher, Alan, Tussiwand, Roxane, Connor, Lisa M., Gallego-Ortega, David, Jankovic, Dragana, Le Gros, Graham, Hepworth, Matthew R., Lamiable, Olivier, and Ronchese, Franca

Published
2022
Full Text
View/download PDF

9. Stromal oncostatin M cytokine promotes breast cancer progression by reprogramming the tumor microenvironment

Author

Araujo, Angela M., Abaurrea, Andrea, Azcoaga, Peio, Lopez-Velazco, Joanna I., Manzano, Sara, Rodriguez, Javier, Rezola, Ricardo, Egia-Mendikute, Leire, Valdes-Mora, Fatima, Flores, Juana M., Jenkins, Liam, Pulido, Laura, Osorio-Querejeta, Inaki, Fernandez-Nogueira, Patricia, Ferrari, Nicola, Viera, Cristina, Martin-Martin, Natalia, Tzankov, Alexandar, Eppenberger-Castori, Serenella, Alvarez-Lopez, Isabel, Urruticoechea, Ander, Bragado, Paloma, Coleman, Nicholas, Palazon, Asis, Carracedo, Arkaitz, Gallego-Ortega, David, Calvo, Fernando, Isacke, Clare M., Caffarel, Maria M., and Lawrie, Charles H.

Subjects
Cytokines -- Genetic aspects -- Health aspects, Oncology, Experimental, Cell differentiation -- Research, Connective tissue cells -- Genetic aspects -- Health aspects, Breast cancer -- Genetic aspects -- Development and progression, Cancer -- Research, Health care industry
Abstract

The tumor microenvironment (TME) is reprogrammed by cancer cells and participates in all stages of tumor progression. The contribution of stromal cells to the reprogramming of the TME is not well understood. Here, we provide evidence of the role of the cytokine oncostatin M (OSM) as central node for multicellular interactions between immune and nonimmune stromal cells and the epithelial cancer cell compartment. OSM receptor (OSMR) deletion in a multistage breast cancer model halted tumor progression. We ascribed causality to the stromal function of the OSM axis by demonstrating reduced tumor burden of syngeneic tumors implanted in mice lacking OSMR. Single-cell and bioinformatic analysis of murine and human breast tumors revealed that OSM expression was restricted to myeloid cells, whereas OSMR was detected predominantly in fibroblasts and, to a lower extent, cancer cells. Myeloid-derived OSM reprogrammed fibroblasts to a more contractile and tumorigenic phenotype and elicited the secretion of VEGF and proinflammatory chemokines CXCL1 and CXCL16, leading to increased myeloid cell recruitment. Collectively, our data support the notion that the stromal OSM/OSMR axis reprograms the immune and nonimmune microenvironment and plays a key role in breast cancer progression., Introduction The tumor microenvironment (TME), composed of different cell types (e.g., fibroblasts, adipocytes, endothelial and infiltrating immune cells), harbors complex cell interactions that are often manipulated and hijacked by tumor [...]

Published
2022
Full Text
View/download PDF

10. MASTL overexpression promotes chromosome instability and metastasis in breast cancer

Author

Rogers, Samuel, McCloy, Rachael A., Parker, Benjamin L., Gallego-Ortega, David, Law, Andrew M. K., Chin, Venessa T., Conway, James R. W., Fey, Dirk, Millar, Ewan K. A., O’Toole, Sandra, Deng, Niantao, Swarbrick, Alexander, Chastain, Paul D., Cesare, Anthony J., Timpson, Paul, Caldon, C. Elizabeth, Croucher, David R., James, David E., Watkins, D. Neil, and Burgess, Andrew

Published
2018
Full Text
View/download PDF

12. Data from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

13. Supplementary Table 1 from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

14. Suppl video 4 from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

15. Suppl video 3 from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

16. Supplementary Data from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

17. Suppl video 1 from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

18. Suppl video 2 from Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, primary, Lin, Francis, primary, Jakovija, Arnolda, primary, Youlten, Scott E., primary, Counoupas, Claudio, primary, Gunzer, Matthias, primary, Bald, Tobias, primary, Woodruff, Trent M., primary, Triccas, James A., primary, Goldstein, Leonard D., primary, Gallego-Ortega, David, primary, Grey, Shane T., primary, and Chtanova, Tatyana, primary

Published
2023
Full Text
View/download PDF

20. Data from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

21. Supplementary Figure 1 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

22. Supplementary Figure 5 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

23. Supplementary Figure 2 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

24. Supplementary Figure 3 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

25. Supplementary Materials and Methods from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

26. Supplementary Figure Legends from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

27. Supplementary Figure 6 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

28. Supplementary Figure 4 from BCL-2 Hypermethylation Is a Potential Biomarker of Sensitivity to Antimitotic Chemotherapy in Endocrine-Resistant Breast Cancer

Author

Stone, Andrew, primary, Cowley, Mark J., primary, Valdes-Mora, Fatima, primary, McCloy, Rachael A., primary, Sergio, C. Marcelo, primary, Gallego-Ortega, David, primary, Caldon, C. Elizabeth, primary, Ormandy, Christopher J., primary, Biankin, Andrew V., primary, Gee, Julia M.W., primary, Nicholson, Robert I., primary, Print, Cristin G., primary, Clark, Susan J., primary, and Musgrove, Elizabeth A., primary

Published
2023
Full Text
View/download PDF

29. Data from Involvement of Lyn and the Atypical Kinase SgK269/PEAK1 in a Basal Breast Cancer Signaling Pathway

Author

Croucher, David R., primary, Hochgräfe, Falko, primary, Zhang, Luxi, primary, Liu, Ling, primary, Lyons, Ruth J., primary, Rickwood, Danny, primary, Tactacan, Carole M., primary, Browne, Brigid C., primary, Ali, Naveid, primary, Chan, Howard, primary, Shearer, Robert, primary, Gallego-Ortega, David, primary, Saunders, Darren N., primary, Swarbrick, Alexander, primary, and Daly, Roger J., primary

Published
2023
Full Text
View/download PDF

30. Supplementary Figures from Involvement of Lyn and the Atypical Kinase SgK269/PEAK1 in a Basal Breast Cancer Signaling Pathway

Author

Croucher, David R., primary, Hochgräfe, Falko, primary, Zhang, Luxi, primary, Liu, Ling, primary, Lyons, Ruth J., primary, Rickwood, Danny, primary, Tactacan, Carole M., primary, Browne, Brigid C., primary, Ali, Naveid, primary, Chan, Howard, primary, Shearer, Robert, primary, Gallego-Ortega, David, primary, Saunders, Darren N., primary, Swarbrick, Alexander, primary, and Daly, Roger J., primary

Published
2023
Full Text
View/download PDF

32. Pleiotrophin drives a prometastatic immune niche in breast cancer

Author

Ganguly, Debolina, primary, Schmidt, Marcel O., additional, Coleman, Morgan, additional, Ngo, Tuong-Vi Cindy, additional, Sorrelle, Noah, additional, Dominguez, Adrian T.A., additional, Murimwa, Gilbert Z., additional, Toombs, Jason E., additional, Lewis, Cheryl, additional, Fang, Yisheng V., additional, Valdes-Mora, Fatima, additional, Gallego-Ortega, David, additional, Wellstein, Anton, additional, and Brekken, Rolf A., additional

Published
2023
Full Text
View/download PDF

33. Neutrophil Conversion to a Tumor-Killing Phenotype Underpins Effective Microbial Therapy

Author

Yam, Andrew O., primary, Bailey, Jacqueline, additional, Lin, Francis, additional, Jakovija, Arnolda, additional, Youlten, Scott E., additional, Counoupas, Claudio, additional, Gunzer, Matthias, additional, Bald, Tobias, additional, Woodruff, Trent M., additional, Triccas, James A., additional, Goldstein, Leonard D., additional, Gallego-Ortega, David, additional, Grey, Shane T., additional, and Chtanova, Tatyana, additional

Published
2023
Full Text
View/download PDF

35. Increased SARS-CoV-2 Infection, Protease, and Inflammatory Responses in Chronic Obstructive Pulmonary Disease Primary Bronchial Epithelial Cells Defined with Single-Cell RNA Sequencing

Author

Johansen, Matt D., primary, Mahbub, Rashad M., additional, Idrees, Sobia, additional, Nguyen, Duc H., additional, Miemczyk, Stefan, additional, Pathinayake, Prabuddha, additional, Nichol, Kristy, additional, Hansbro, Nicole G., additional, Gearing, Linden J., additional, Hertzog, Paul J., additional, Gallego-Ortega, David, additional, Britton, Warwick J., additional, Saunders, Bernadette M., additional, Wark, Peter A., additional, Faiz, Alen, additional, and Hansbro, Philip M., additional

Published
2022
Full Text
View/download PDF

37. Stromal oncostatin M cytokine promotes breast cancer progression by reprogramming the tumor microenvironment

Author

Bioquímica y biología molecular, Biokimika eta biologia molekularra, Araujo, Angela M., Abaurrea, Andrea, Azcoaga, Peio, López Velazco, Joanna I., Manzano, Sara, Rodríguez Martínez, Javier, Rezola, Ricardo, Egia Mendikute, Leire, Valdés Mora, Fátima, Flores, Juana M., Jenkins, Liam, Pulido, Laura, Osorio Querejeta, Iñaki, Fernández Nogueira, Patricia, Ferrari, Nicola, Viera, Cristina, Martín Martín, Natalia, Tzankov, Alexandar, Eppenberger Castori, Serenella, Álvarez López, Isabel, Urruticoechea, Ander, Bragado, Paloma, Coleman, Nicholas, Palazón, Asís, Carracedo Pérez, Arkaitz, Gallego Ortega, David, Calvo González, Fernando, Isacke, Clare M., Caffarel, María M., Lawrie, Charles, Bioquímica y biología molecular, Biokimika eta biologia molekularra, Araujo, Angela M., Abaurrea, Andrea, Azcoaga, Peio, López Velazco, Joanna I., Manzano, Sara, Rodríguez Martínez, Javier, Rezola, Ricardo, Egia Mendikute, Leire, Valdés Mora, Fátima, Flores, Juana M., Jenkins, Liam, Pulido, Laura, Osorio Querejeta, Iñaki, Fernández Nogueira, Patricia, Ferrari, Nicola, Viera, Cristina, Martín Martín, Natalia, Tzankov, Alexandar, Eppenberger Castori, Serenella, Álvarez López, Isabel, Urruticoechea, Ander, Bragado, Paloma, Coleman, Nicholas, Palazón, Asís, Carracedo Pérez, Arkaitz, Gallego Ortega, David, Calvo González, Fernando, Isacke, Clare M., Caffarel, María M., and Lawrie, Charles

Abstract

[EN] The tumor microenvironment (TME) is reprogrammed by cancer cells and participates in all stages of tumor progression. The contribution of stromal cells to the reprogramming of the TME is not well understood. Here, we provide evidence of the role of the cytokine oncostatin M (OSM) as central node for multicellular interactions between immune and nonimmune stromal cells and the epithelial cancer cell compartment. OSM receptor (OSMR) deletion in a multistage breast cancer model halted tumor progression. We ascribed causality to the stromal function of the OSM axis by demonstrating reduced tumor burden of syngeneic tumors implanted in mice lacking OSMR. Single-cell and bioinformatic analysis of murine and human breast tumors revealed that OSM expression was restricted to myeloid cells, whereas OSMR was detected predominantly in fibroblasts and, to a lower extent, cancer cells. Myeloid-derived OSM reprogrammed fibroblasts to a more contractile and tumorigenic phenotype and elicited the secretion of VEGF and proinflammatory chemokines CXCL1 and CXCL16, leading to increased myeloid cell recruitment. Collectively, our data support the notion that the stromal OSM/OSMR axis reprograms the immune and nonimmune microenvironment and plays a key role in breast cancer progression.

Published
2022

38. Stromal oncostatin M cytokine promotes breast cancer progression by reprogramming the tumor microenvironment

Author

Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Eusko Jaurlaritza, Asociación Española Contra el Cáncer, European Commission, European Research Council, Ministerio de Economía y Competitividad (España), Fundación la Caixa, Cancer Council NSW (Australia), BBVA, Araujo, Angela M., Abaurrea, Andrea, Azcoaga, Peio, López-Velazco, Joanna I., Manzano, Sara, Rodríguez, Javier, Rezola, Ricardo, Egia-Mendikute, Leire, Valdés-Mora, Fátima, Flores, Juana M., Jenkins, Liam, Pulido, Laura, Osorio-Querejeta, Iñaki, Fernández-Nogueira, Patricia, Ferrari, Nicola, Viera, Cristina, Martín-Martín, Natalia, Tzankov, Alexandar, Eppenberger-Castori, Serenella, Álvarez-López, Isabel, Urruticoechea, Ander, Bragado, Paloma, Coleman, Nicholas, Palazón, Asís, Carracedo, Arkaitz, Gallego-Ortega, David, Calvo, Fernando, Isacke, Clare M., Caffarel, María M., Lawrie, Charles H., Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Eusko Jaurlaritza, Asociación Española Contra el Cáncer, European Commission, European Research Council, Ministerio de Economía y Competitividad (España), Fundación la Caixa, Cancer Council NSW (Australia), BBVA, Araujo, Angela M., Abaurrea, Andrea, Azcoaga, Peio, López-Velazco, Joanna I., Manzano, Sara, Rodríguez, Javier, Rezola, Ricardo, Egia-Mendikute, Leire, Valdés-Mora, Fátima, Flores, Juana M., Jenkins, Liam, Pulido, Laura, Osorio-Querejeta, Iñaki, Fernández-Nogueira, Patricia, Ferrari, Nicola, Viera, Cristina, Martín-Martín, Natalia, Tzankov, Alexandar, Eppenberger-Castori, Serenella, Álvarez-López, Isabel, Urruticoechea, Ander, Bragado, Paloma, Coleman, Nicholas, Palazón, Asís, Carracedo, Arkaitz, Gallego-Ortega, David, Calvo, Fernando, Isacke, Clare M., Caffarel, María M., and Lawrie, Charles H.

Abstract

The tumor microenvironment (TME) is reprogrammed by cancer cells and participates in all stages of tumor progression. The contribution of stromal cells to the reprogramming of the TME is not well understood. Here, we provide evidence of the role of the cytokine oncostatin M (OSM) as central node for multicellular interactions between immune and nonimmune stromal cells and the epithelial cancer cell compartment. OSM receptor (OSMR) deletion in a multistage breast cancer model halted tumor progression. We ascribed causality to the stromal function of the OSM axis by demonstrating reduced tumor burden of syngeneic tumors implanted in mice lacking OSMR. Single-cell and bioinformatic analysis of murine and human breast tumors revealed that OSM expression was restricted to myeloid cells, whereas OSMR was detected predominantly in fibroblasts and, to a lower extent, cancer cells. Myeloid-derived OSM reprogrammed fibroblasts to a more contractile and tumorigenic phenotype and elicited the secretion of VEGF and proinflammatory chemokines CXCL1 and CXCL16, leading to increased myeloid cell recruitment. Collectively, our data support the notion that the stromal OSM/OSMR axis reprograms the immune and nonimmune microenvironment and plays a key role in breast cancer progression.

Published
2022

39. ALTEN: A High‐Fidelity Primary Tissue‐Engineering Platform to Assess Cellular Responses Ex Vivo

Author

Law, Andrew M. K., primary, Chen, Jiamin, additional, Colino‐Sanguino, Yolanda, additional, Fuente, Laura Rodriguez de la, additional, Fang, Guocheng, additional, Grimes, Susan M., additional, Lu, Hongxu, additional, Huang, Robert J., additional, Boyle, Sarah T., additional, Venhuizen, Jeron, additional, Castillo, Lesley, additional, Tavakoli, Javad, additional, Skhinas, Joanna N., additional, Millar, Ewan K. A., additional, Beretov, Julia, additional, Rossello, Fernando J., additional, Tipper, Joanne L., additional, Ormandy, Christopher J., additional, Samuel, Michael S., additional, Cox, Thomas R., additional, Martelotto, Luciano, additional, Jin, Dayong, additional, Valdes‐Mora, Fatima, additional, Ji, Hanlee P., additional, and Gallego‐Ortega, David, additional

Published
2022
Full Text
View/download PDF

40. Pleiotrophin drives a pro-metastatic immune niche within the breast tumor microenvironment

Author

Ganguly, Debolina, primary, Schmidt, Marcel O, additional, Coleman, Morgan, additional, Ngo, Tuong-Vi, additional, Sorrelle, Noah, additional, Dominguez, Adrian TA., additional, Toombs, Jason, additional, Lewis, Cheryl, additional, Fang, Yisheng, additional, Mora, Fa Valdes, additional, Gallego-Ortega, David, additional, Wellstein, Anton, additional, and Brekken, Rolf A, additional

Published
2022
Full Text
View/download PDF

41. Inflammatory blood neutrophils in COPD stem from activated bone marrow progenitors

Author

Kapellos, Theodore, primary, Bassler, Kevin, additional, Fujii, Wataru, additional, Pecht, Tal, additional, Bonaguro, Lorenzo, additional, Galvao, Izabela, additional, Saglam, Adem, additional, Dudkin, Erica, additional, Frishberg, Amit, additional, De Domenico, Elena, additional, Horne, Arik, additional, Donovan, Chantal, additional, Kim, Richard Y., additional, Gallego-Ortega, David, additional, Becker, Matthias, additional, Händler, Kristian, additional, Ulas, Thomas, additional, Hasenauer, Jan, additional, Pizarro, Carmen, additional, Hansbro, Phil M., additional, Skowasch, Dirk, additional, and Schultze, Joachim L., additional

Published
2022
Full Text
View/download PDF

42. Additional file 7 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 7: Fig. S5. Effect of PD-L1 and MT Oas2 on primary tumor initiation and expansion in parous mice. Examples of tumor growth curves of individual mice. The 2nd/3rd and 4th mammary glands were palpated twice weekly and tumor growth was estimated by measurement of the major and minor axis of individual tumors using calipers. Y axis is placed at the day of tumor detection on the x axis as day 0. Black diamonds show a treatment with IP IgG or PD-L1 antibodies and red diamonds indicate parity. WT = wild type, MT = MT Oas2 MT/MT = homozygous MT Oas2.

Published
2022
Full Text
View/download PDF

43. Additional file 8 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 8: Fig. S6. Effect of PD-L1 and MT Oas2 on primary tumor initiation and expansion in parous mice. Two-way Kaplan–Meier survival analysis for the indicated periods and endpoints, and for the indicated genotypes and treatments (PD-L1 or IgG IP), of mouse cohorts. P values and hazard ratios (HR) calculated by the log-rank test using GraphPad Prism. n = 8 WT PD-L1, 8 WT IgG, 14 MT PD-L1, 15 MT IgG.

Published
2022
Full Text
View/download PDF

44. Additional file 6 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 6: Fig. S4. Flow cytometry gating strategy for monocytes and neutrophils known as myeloid-derived suppressor cells. Series of gates used to quantify monocytes and neutrophils using flow cytometry.

Published
2022
Full Text
View/download PDF

45. Additional file 5 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 5: Fig. S3. Effects of MT Oas2 on T cells in parous mice. A screening of various T-cell parameters was undertaken in mammary tumors, lymph nodes, spleen and thymus. Error bars are standard error of the mean and p values calculated by Student’s t test (all non-significant). Axes represent % of total cells passing the previous gate. Genotypes, WT/WT homozygous wildtype Oas2, MT/MT homozygous mutant Oas2.

Published
2022
Full Text
View/download PDF

46. Additional file 1 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 1: Table S1. Antibodies, concentration and antigen retrieval conditions for immunohistochemistry. All reagents were from Leica BOND or DAKO for automated or manual IHC (as specified). Visualization of antigen–antibody complexes was performed using the DAB + liquid substrate chromogen system (K3467).

Published
2022
Full Text
View/download PDF

47. Additional file 3 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 3: Fig. S1. Effect of MT Oas2 on primary tumor expansion in parous mice. Examples of tumor growth curves of individual mice. The left- and right-hand side 2nd/3rd and 4th mammary glands were palpated twice weekly and tumor growth was estimated by measurement of the major and minor axis of each gland using calipers. Y axis is placed at parity indicated on the x axis as day 0. Numbers show the total number of glands with tumors detected, the number that showed a period of no growth, and the number that showed regression at or following parity. W, wild type; M, MT; W/W, homozygous wild type; M/M, homozygous MT.

Published
2022
Full Text
View/download PDF

48. Additional file 4 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Subjects
reproductive and urinary physiology
Abstract

Additional file 4: Fig. S2. Effect of MT Oas2 on primary tumor initiation and expansion in nulliparous and parous mice. Two-way Kaplan–Meier survival analysis for the indicated periods and endpoints, and for the indicated genotypes, WT wildtype Oas2, MT mutant Oas2, and parity status of mouse cohorts (20 per group). P values and hazard ratios (HR) calculated by the log-rank test using GraphPad Prism. n = 19 MT parous, 21 WT parous, 20 MT nulliparous and 19 WT nulliparous.)

Published
2022
Full Text
View/download PDF

49. Additional file 2 of Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases

Author

Ho, Wing-Hong Jonathan, Law, Andrew M. K., Masle-Farquhar, Etienne, Castillo, Lesley E., Mawson, Amanda, O’Bryan, Moira K., Goodnow, Christopher C., Gallego-Ortega, David, Oakes, Samantha R., and Ormandy, Christopher J.

Abstract

Additional file 2: Table S2. Antibodies used for flow cytometry. Panel name, antibody name by antigen recognized and conjugated fluorochrome, the antibody supplier and catalogue number and final diluted concentration for each is shown.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results