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The poultry industry is generating a significant amount of waste from chicken droppings that are abundant in microbes as well as macro- and micronutrients suitable for manure. It has the potential to improve the microbial activity and nutrient dynamics in the soil, ultimately improving soil fertility. The present study aimed to investigate the effect of chicken droppings manure (CDM) on the diversity of the soil microbiome in the free walking chicken's area located in Stefanidar, Rostov Region, Russia. The data obtained were compared with 16 s rRNA from control samples located not far from the chicken's free-walking area, but not in direct contact with the droppings. Effect of CDM on the physicochemical characteristics of the soil and changes in its microbial diversity were assessed by employing the metagenomic approaches and 16 s rRNA-based taxonomic assessment. The alpha and beta diversity indices revealed that the application of the CDM significantly improved the soil microbial diversity. The 16S taxonomical analysis confirmed Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Planctomycetes as abundant bacterial phylum. It also revealed the increase in the total number of the individual operational taxonomic unit (OTU) species, a qualitative indicator of the rich microbial community. The alpha diversity confirmed that the significant species richness of the soil is associated with the CDM treatment. The increased OTUs represent the qualitative indicator of a community that has been studied up to the depth of 5-20 cm of the CDM treatment range. These findings suggested that CDM-mediated microbial richness are believed to confer the cycling of carbon, nitrogen, and sulfur, along with key soil enzymes such as dehydrogenases and catalase carbohydrate-active enzymes. Hence, the application of CDM could improve soil fertility by nutrient cycling caused by changes in soil microbial dynamics, and it could also be a cost-effective sustainable means of improving soil health.

The nature of the mineral supply of certain biogeochemical provinces and their correlative interactions within biological organisms determine the degree of development of immunosuppressive states in animals. So we studied the level of minerals in the samples of hair and blood in cattle in the «mother-offspring» system and established the nature of the correlation of cobalt, zinc, iron and copper. It has been proven that low levels of cobalt (cows - 429.30 ± 4.05 nmol/l and calves - 430.80 ± 5.60 nmol / L) and zinc (cows - 7.21 ± 0.20 pmol/l and calves - 7.60 ± 0.15 pmol/l) against the threshold values of iron (cows - 17.90 ± 1.30 pmol/l and calves - 18.40 ± 1.80 pmol/l) and copper (cows - 13.90 ± 1.80 pmol/l and calves - 14.08 ± 1.05 pmol/l) in the blood caused hematopoietic function impairment and led to the development of mild hypochromic anemia (erythrocytes: cows - 6.01 ± 0.40x1012/l and calves - 6.36±0.50x1012/l) in animals in the «mother-offspring» system. In addition, metabolic disorders caused by nutritional deficiency of zinc contributed to a decrease in the synthesis of alkaline phosphatase (cows - 87.90 ± 5.10 U/l and calves - 126.80±4.02 U/l), exacerbating the disorder of erythropoietic function of red bone animal brain. A long-term imbalance of copper and zinc in the nutrient substrates of the studied animals was revealed, as evidenced by the results of a mineralogram of hairline samples (copper: cows - 13.910 ± 0.67 mg/kg and calves - 12.609 ± 0.70 mg/kg; zinc: cows - 57.960 ± 0.90 mg/kg and calves 60.315 ± 0.99 mg/kg), which ultimately led to a deficiency of zinc in the body, leading to the development of an immunosuppressive state (Ig M: cows - 0.99 ± 0.15 mg/ml and calves - 1.64 ± 0.05 mg/ml; Ig A: cows - 0.30 ± 0.05 mg/ml and calves - 2.05 ± 0.10 mg/ml).

The aim of the study was to study the effect of diets using the potentially probiotic drug Bacillus amyloliquefaciens KB54 as part of compound feed (the rate of administration is 0.1% of the total diet, the content of viable spores in the final product is 106CFU/g) in the diets of laying hens of the Dekalb cross. In the course of the study, it was established that the viability indicators of laying hens were at a high level and the safety of livestock in the studied groups during the period of the experiment was 100%; egg production in the experimental group was 96.7%, which is 2.3% more than in the control group of laying hens; chickens in the experimental group exceeded the control in growth intensity, which affected an increase in live weight by 12.5%, or 188.5 grams, which is 1.7 percentage points or 25.2 grams more than in the control group; feed costs for 10 eggs and 1 kg of egg mass in the experimental group were 1.43 and 2.36 kg, respectively, which is 11.7 and 14.5% lower than the control values. In this regard, we recommend using the probiotic preparation Bacillus amyloliquefaciens KB54 in the diets of laying hens, introduced into the compound feed in the amount of 0.1% of the total diet with the content of viable spores in the final product 106CFU/g.

The comorbidity of babesiosis and dirofilariasis in dogs is an important clinical problem, despite the significant achievements of recent years in understanding the pathogenesis of this mixed invasion. It has been established that the leading pathogenetic component in the development of the cytolytic syndrome with this comorbidity in dogs is oxidative stress resulting from the mismatch of the prooxidant and antioxidant resources of the cell under the influence of Babesia canis parasitism. On the basis of morphological, biochemical and ultrasonographic studies, a direct correlation was found between oxidative stress and hepatoprivial syndrome in dogs with comorbidity of babesiosis and dirofilariasis. Hepatoprivial syndrome was accompanied by the development of hypochromic anaemia, leukocytosis, hyperproteinemia, hypoglycemia, a disorder of pigment metabolism and an increase in the catalytic activity of serum enzymes, which indicated a violation of the metabolic activity of the liver and damage to its parenchyma. The activation of lipid peroxidation processes in the hepatocytes contributed to an increase in the catalytic activity of the blood serum enzymes in sick animals, and to a decrease in the antioxidant defence of sick dogs, due to a relative decrease in the level of vitamin A in the blood. Thus, the comorbidity of babesiosis and dirofilariasis in dogs enhances the oxidative syndrome that underlies the pathogenetic mechanisms of this mixed invasion, thereby increasing the degree of involvement in the pathological process of the liver, which is manifested by hepatoprivial syndrome. Keywords: oxidative stress, hepatoprivial syndrome, dirofilariasis, babesiosis, mixtinvasion, dogs

The fulfillment of a number of the most important physiological functions of the skin is directly related to skin lipids. This article presents the results of studying the lipid-containing and lipid-synthesizing structures of the young Poltava Meat Breed boars’ skin. Morphofunctional epidermis features, sebaceous glands and adipose tissue cells of the hypodermis and their relationship with other skin structures were studied. The boars of Poltava Meat Breed, upon reaching the live weight of 100 kg, have certain specific features of skin structure, which plays an important role for developing efficient methods of selectioning, breeding and keeping the animals, which would raise the functional abilities of highly productive animals and help obtaining high-quality production from them.

The distribution of feed containing probiotics is one of the most attractive trends in modern animal husbandry for consumers. The introduction of a live component makes the feed not only more effective, but also reduces the content of toxic components, making animal products more environmentally friendly. However, the introduction of viable cells of beneficial microorganisms into the feed significantly increases the requirements for the level of feed design, which in turn forces the development of new, unusual algorithms for such design for zootechnics. First of all, the development of feed additives with the inclusion of probiotic strains should be carried out only taking into account the specific application (type of animal of agricultural significance) and the extent to which the studied microorganisms and the substrate for microbial fermentation are suitable for this animal. The most important issue in this regard is the interaction of the living component with the inanimate one. At a minimum, substances that are part of probiotic feeds and feed additives should not inhibit the growth of target microorganisms. The ideal option is the ability to stimulate their growth or prebiotic activity.

The aim of the study was to develop an algorithm for compounding feed mixtures with the inclusion of probiotic strains. The authors have developed the basic principles underlying the modeling of feed additive formulations. However, the introduction of viable cells of beneficial microorganisms (probiotics) into the feed significantly increases the requirements for the level of design of feed products. Thus, the article describes the basic principles of the algorithm for designing feed products for poultry. A technological scheme for combining the components of the supplement and probiotic drugs has been developed. In accordance with the developed algorithm, the effect of such an active component of the feed additive as bentonite on the growth and survival of the target probiotic strains was tested. Feed mixtures with the inclusion of the probiotic strains under study were tested in the experiment on broiler chickens. The result of the study was positive results of the productivity of the raised poultry in the experimental groups.

Our research in the field of developing the formula of feed mixtures with the inclusion of probiotic strains is relevant and in demand. This is due to the current consumer trends on the environmental friendliness and organic nature of the livestock and poultry products produced. The authors have developed the basic principles underlying the modeling of feed additive formulations. However, the introduction of viable cells of beneficial microorganisms (probiotics) into the feed significantly increases the requirements for the level of design of feed products. Thus, the article describes the basic principles of the algorithm for designing feed products for poultry. A technological scheme for combining the components of the supplement and probiotic preparations immediately before feeding to poultry has been developed. According to the developed algorithm, the effect of such an active component of the feed additive as bentonite on the growth and survival of the target probiotic strains was tested. As a result of the conducted research, an algorithm for obtaining new feed additives for poultry is presented. The parameters of feeding of repair young animals and laying hens have been developed. The composition of feed additives with the inclusion of the probiotic strains under study was developed and studied.

The greatest success in improving the breeding and productive qualities of Hereford cattle can be achieved through the use of high-value breeding bulls. The role of producers at the modern stages of beef cattle breeding has increased dramatically, so the evaluation of bulls by the quality of offspring has become an important breeding event to improve and create highly productive herds of beef cattle. At the same time, the use of bulls that are predisposed to various diseases or the deterioration of their offspring can cause irreparable negative consequences for beef cattle breeding. In the course of research work in the conditions of the farm, studies were conducted to assess the quality of bulls-producers of the Hereford breed of cattle. Evaluation of Hereford bulls by the quality of offspring in farm conditions will increase the efficiency of herd reproduction technology in beef cattle breeding, allow rational use of bulls, get a high yield of calves (90-95%), organize the accuracy of accounting for the origin of young animals, increase the level of breeding work to improve the productive, breeding qualities of animals and create highly productive herds of Hereford cattle in a shorter time.

The study was conducted to identify the early use of young animals (gobies and heifers) in cattle for breeding purposes. The condition under which the research was carried out was intensive and traditional rearing of young cattle. In the experimental groups, feeding was intense, and in the control groups – according to the accepted technology in the farm. Young growth was carried out until the age of 15 months. Under such different growing conditions, we have established the features of the formation of reproductive organs in gobies and heifers. The good development of the genitals of the heifers of the experimental group, and especially the ovaries, made it possible to carry out their fruitful insemination in the period from 13 to 14 months of age. For this, two bulls were selected from the experimental group at the age of 12-13 months, which, by manual mating, inseminated 20 heifers for a month. During a rectal examination, they turned out to be all pregnant and in due time, without complications, became hotels, gave normal viable offspring. Calving calves aged 22-23 months during 1 lactation they were in no way inferior in milk production to heifers inseminated at the age of 24-28 months and calving at 33-37 months.

The greatest success in improving the breeding and productive qualities of Hereford cattle can be achieved through the use of high-value breeding bulls. The role of producers at the modern stages of beef cattle breeding has increased dramatically, so the evaluation of bulls by the quality of offspring has become an important breeding event to improve and create highly productive herds of beef cattle. At the same time, the use of bulls that are predisposed to various diseases or the deterioration of their offspring can cause irreparable negative consequences for beef cattle breeding. In the course of research work in the conditions of the farm, studies were conducted to assess the quality of bulls-producers of the Hereford breed of cattle. Evaluation of Hereford bulls by the quality of offspring in farm conditions will increase the efficiency of herd reproduction technology in beef cattle breeding, allow rational use of bulls, get a high yield of calves (90-95%), organize the accuracy of accounting for the origin of young animals, increase the level of breeding work to improve the productive, breeding qualities of animals and create highly productive herds of Hereford cattle in a shorter time.

Studies have established the effect of ekobentokorm (TU 9283-199-10514645-13-2013) on the metabolism of cows and the natural resistance of young black-and-white breed. The physiological state of cows was assessed by morphological and biochemical studies of blood, in which changes in the body’s metabolic processes were tracked in dynamics according to the following indicators: the number of red blood cells and hemoglobin level, total protein and protein fractions, glucose, urea, aminotransferases (AST, ALT), total calcium and inorganic phosphorus, trace elements (Mg, Zn, Fe, Cu) and carotene. The indicators characterizing the natural resistance of young animals were studied: red blood cells, white blood cells, hemoglobin, color index, total protein and protein fractions, phagocytic activity of neutrophil granulocytes, bactericidal and lysozyme activity of blood serum, the content and concentration of T-and B-lymphocytes, the level of circulating immune complexes (CIC). As a result of the conducted research, it was found that ekobentokorm has high biological activity, improves morphological and biochemical parameters of cows blood, and also increases milk productivity. It has a pronounced stimulating effect on the immunocompetent systems of the body of young animals, as a result of which the level of non-specific protection of the animal increases.

In view of the potential global human factors threats, NBIC convergence in manufacturing systems requires new, sustainable approaches to HR management. The proposed methodology is based on the systems paradigm of Janos Kornai, which is used in the framework of BigData technologies, artificial intelligence, and blockchain. The problem of control, optimization and evolution of the human factor in NBIC-production cyber-physical systems is solved by means of the developed human-oriented concept of HR-resources management, the development of which occurs in a spiral, involving exit from the system to form new meanings and concepts. Each turn of the spiral is a self-organizing system. For the current stage of development, a cyber-physical system of interaction “Man” - “Machine” is proposed, based on global and double iterative connections, parallel analysis, scenario forecasting, machine learning, cognitive component and collective decision making. The developed human-oriented concept of HR-resources management allows you to control, optimize and develop human production activities, ensure iterative self-development and self-improvement of the system, guarantee the stability and safety of the system, maximize the percentage of return on human resources and reduce the level of opportunistic human behavior.

Studies of the structure of the interaction of signs with the help of factor analysis is based on the idea of the complex nature of the phenomenon being studied, expressed in the interdependence of individual signs, determined by “internal” hidden causes, forming the specifics of a property, quality. We used the factor analysis method to study the producers’ assessments of the quality of the offspring and to test their bullsons for their own productivity of the Kalmyk and Hereford cattle breeds. The analysis included material from the work of pedigree reproducers and plants in the Kalmyk and Hereford breeds of the Rostov Region. The total number of animals included in the treatment was 96 manufacturing bulls and 272 sons bulls. As a result of the studies, the proposed breeding indices on one basis are quite suitable for the preliminary selection of bullcalves in commodity farms or in pedigree farmers for breeding beef cattle. In order to get a final assessment, we compiled new breeding indices, including two (live weight at 8 and 15 months, live weight at 15 months and average daily gain from 8 to 15 months), as well as three characteristics (live weight in 8, 15 months and average daily gain). These signs are objective, easy to account. According to the estimates obtained for individual bull-sons, it is possible by summing up the values of new factors to identify the bulls’ assessment of the quality of the offspring. Analysis of assessment materials is characterized by high reliability (P > 0.999).

The paper reveals the current problems of production systems, their lack of readiness to function in the conditions of application and development of NBIC technologies. The methodological basis of the research consists of fundamental works and modern scientific and practical developments of national and foreign scientists on the problems of creation and functioning of production, technological and socio-economic systems in the conditions of development using NBIC technologies. The research was carried out using such general scientific and special methods as: historical-dialectical and abstract-logical; comparative-analytical and system analysis; synthesis of scientific approaches. The authors prove the need to create a new system paradigm. The new paradigm of production systems that realize the potential of NBIC technologies is based on a concept that finds its concrete expression, firstly, in the initial or original concepts, and secondly, in the system of basic principles, laws, or sufficiently plausible hypotheses. It is these elements that make up the conceptual core of the paradigm, and efforts should be directed to their search and justification when forming a new paradigm. The paper analyzes the main methods and tools for implementing a new approach to the design of production systems.

Clinical and immunological parallels in inflammatory periodontal diseases are considered taking into account some features of the functioning of general and local structures of the immune system in periodontitis of varying severity, chronic generalized periodontitis, aggressive periodontitis. In the analysis of immunopathogenesis of inflammatory periodontal diseases, an essential role is given to an imbalance in the immune and cytokine system.

Assessment of the effectiveness of treatment and recovery of reproductive functions of cows with fatty liver decease using a drug based on a healthy liver hydrolysate (the enzyme structures of the protein after acid treatment and centrifugation remain in the sediment of the tissue substrate, and their released amino acids become constituent components of the over-sediment solution of the hydrolysate). Two groups of animals of 15 heads each were formed: first-calf heifers entered the first group on the first day after calving, liver pathology was indicated by an increase in its borders during percussion, and animals with clinical signs of deep fatty liver disease were included in the second group a month after calving. The dose was 40 ml daily (20 ml intramuscularly and 20 ml subcutaneously), the course of treatment was 5 days. After the 1st stage, the remaining 25 cows of both groups were considered as one experimental group of the 2nd stage, which was periodically monitored until the next calving and insemination; the control was 25 animals not subjected to hepatoprotective therapy. Cows that underwent even one treatment procedure were better adapted to the newborn period and subsequent insemination. They have faster involution of the uterus and the resumption of the sexual cycle (P