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Submitted by G??bia Leite (gabya.leite@gmail.com) on 2016-07-11T14:08:17Z No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-14T12:22:58Z (GMT) No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-14T12:28:44Z (GMT) No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Made available in DSpace on 2016-07-14T12:28:44Z (GMT). No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Previous issue date: 2014-06-13 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior Galeno D.M.L. Effects of Eugenia punicifolia (Khunt ) DC related to metabolic syndrome in experimental models . Manaus, 2014. 133p. Thesis (Ph.D.) - Graduate Program Multi - Institutional Biotechnology, Federal University of Amazonas. The Eugenia punicifolia species is a plant popularly known as "pedra-ume-caa", widely distributed in the Amazon region. In the state of Amazonas, this species has been widely used in folk medicine to treat inflammatory and hyperglycemic disorders. However, studies involving other parameters found in the metabolic syndrome (MS) have not yet been elucidated. The MS is characterized by a complex disorder, represented by a number of cardiovascular risk factors involving central adiposity, hypertension, endothelial dysfunction and insulin resistance. Studies have shown that nutritional supplementation with plants that effectively address the pathogenic mechanisms of this syndrome represents an effective improvement for the problem. This project aim was to analyze biological activities in vitro and in vivo of the aqueous extract of the leaves of E. punicifolia (EEP), emphasizing the inhibitory activity digestive enzymes, angiotensin converting enzyme (ACE) inhibitor, antioxidants, hypolipemiant, cytotoxic and anti antihypertensive effects. Enzyme inhibition assays, as well as the determination of the ACE activity in Wistars rats serum were performed by the fluorometric method and the indirect tests of cell viability, antioxidants, determining the presence of flavonoids and phenolic compounds were performed by colorimetric assays. The evaluation of the EEP on blood pressure parameters was performed by using five experimental groups, which were randomly divided into: normotensive group (2R), the hypertensive group (2R1C), enalapril hypertensive group (ENAL), extract hypertensive group 150 mg/kg/day (EEP150) and extract hypertensive group 300 mg/kg/dia (EEP300). The effects of EEP in systolic pressure were acquired indirectly by tail plethysmography in conscious rats. Biochemical parameters were measured in rat???s plasma through appropriate kits. The study has shown, for the first time, that EEP exhibited scavenging activity on ABTS??+, DPPH??, and O2??- (IC50=10.5 ?? 1.2; 28.84 ?? 0.54 and 38.12 ?? 2.6 ??g/mL, respectively). The EEP also exhibited cellular antioxidant activity in vitro concentration-dependent manner, and did not show cell cytotoxic effects. Furthermore, the EEP inhibited ACE activity (70.8%) using the concentration of 0.3 mg/mL (IC50 of 24.3 ?? 0.7 mg/mL), and inhibited the activities of the enzymes ??-amylase (IC50 = 122.8 ?? 6.3 mg/mL), ??-glucosidase (IC50 = 2.9 ?? 0.1 mg/mL) and xanthine oxidase (IC50 = 23.5 ?? 2.6 mg/mL). Both the EEP150 (21.7%) and the EEP300 (16.8%) reduced systolic blood pressure. The hypotensive effect of EEP correlated with the reduction of plasma ACE activity and also reduced total cholesterol (48%), triglycerides (52%), VLDL (52%) and LDL (55%). In addition, the EEP wasn???t cause renal, hepatic or neurotoxic acute or chronic toxicity. The EEP showed antioxidants, hypotensive, hypolipidemic effects and digestive enzymes and ACE were inhibited. These activities are probably related to the presence of antioxidant such as phenols and flavonoids compounds present in the extract, which act by inhibiting key enzymes of carbohydrate metabolism and angiotensin converting enzyme. These findings may support further clinical studies to further evaluate the potential of EEP on the SM. Galeno, D.M.L. Efeitos da Eugenia punicifolia (Khunt) DC em modelos experimentais relacionados ?? S??ndrome Metab??lica. Manaus, 2014.133p. Tese (doutorado)- Programa de P??s-graduac??o Multi-Institucional em Biotecnologia da Universidade Federal do Amazonas A esp??cie Eugenia punicifolia ?? uma planta popularmente conhecida como "pedra-ume-ca?????, distribu??da amplamente na regi??o amaz??nica. No estado do Amazonas, essa esp??cie tem sido bastante utilizada na medicina popular para tratar dist??rbios hiperglic??micos e inflamat??rios. No entanto, estudos envolvendo outros par??metros encontrados na s??ndrome metab??lica (SM) ainda n??o foram elucidados. A SM se caracteriza por um transtorno complexo, representado por um conjunto de fatores de risco cardiovascular que envolve deposi????o central de gordura, hipertens??o arterial, disfun????o endotelial e resist??ncia ?? insulina. Estudos t??m evidenciado que a suplementa????o nutricional com plantas que atuem efetivamente com os mecanismos patog??nicos desta s??ndrome representa um potencial eficaz para o problema. Este projeto teve como objetivo analisar atividades biol??gicas in vitro e in vivo do extrato aquoso das folhas da E. punicifolia (EEP), enfatizando a atividade inibit??ria de enzimas digest??rias, enzima conversora de angiotensina (ECA), efeitos antioxidantes, hipolipemiantes, citot??xicos e anti-hipertensivos. Os ensaios de inibi????o enzim??tica, assim como a determina????o da atividade da ECA no soro de ratos wistars foram realizados utilizando um m??todo indireto fluorim??trico e os testes de viabilidade celular, antioxidantes, determina????o da presen??a de compostos fen??licos e flavonoides por meio de ensaios colorim??tricos. A avalia????o do EEP sobre os par??metros press??ricos foi realizada mediante a utiliza????o de cinco grupos experimentais, os quais foram divididos randomicamente em: Grupo normotenso (2R), Grupo hipertenso (2R1C), Grupo hipertenso enalapril (ENAL), Grupo hipertenso extrato 150 mg/kg/dia (EEP150) e Grupo hipertenso extrato 300 mg/kg/dia (EEP300). Os efeitos do EEP na press??o sist??lica foram adquiridos de forma indireta por plestimografia de cauda em ratos conscientes. Os par??metros bioqu??micos foram avaliados no plasma dos ratos por meio de kits apropriados. Nesse estudo foi mostrado, pela primeira vez, que o EEP apresentou atividade sequestradora de radicais livres nos testes de ABTS??+, DPPH?? e O2??- (CI50=10,5 ?? 1,2; 28,84 ?? 0,54; e 38,12 ?? 2,6 ??g/mL, respectivamente). O EEP tamb??m exibiu atividade antioxidante celular in vitro de maneira concentra????o-dependente, al??m de n??o evidenciar efeitos citot??xicos celulares. Ademais, o EEP inibiu 70,8% a atividade da ECA na concentra????o de 0,3 mg/mL (CI50 de 24 ?? 0,7 ??g/mL), inibiu as atividades das enzimas ??-amilase (CI50=122,8 ?? 6,3 ??g/mL), ??-glucosidase (CI50= 2,9 ?? 0,1 ??g/mL) e xantina oxidase (CI50= 23,5 ?? 2,6 ??g/mL). O EEP150 reduziu a press??o sist??lica em 21,7% e o EEP300 em 16,8%. O efeito hipotensor do EEP se correlacionou com a redu????o da atividade da ECA no plasma e tamb??m diminuiu o colesterol total (48%), triglicer??deos (52%), VLDL (52%) e LDL (55%). Al??m disso, o EEP n??o causou toxicidade cr??nica e nem aguda renal, hep??tica ou neurot??xica. O EEP mostrou atividades antioxidantes, hipotensores, hipolipemiantes e inibiu as enzimas digest??rias e a ECA. Essas atividades provavelmente estejam relacionadas ?? presen??a de compostos antioxidantes, como fen??is e flavonoides presentes no extrato, que atuam inibindo enzimas chaves do metabolismo dos carboidratos e a enzima conversora de angiotensina. Estes achados podem fundamentar estudos cl??nicos posteriores para melhor avaliar o potencial do EEP sobre a SM.

Made available in DSpace on 2015-04-11T13:38:30Z (GMT). No. of bitstreams: 1 Capa da Dissertacao Georgia.pdf: 241136 bytes, checksum: 26172cab25063b953eff0455c1729780 (MD5) Previous issue date: 2013-05-21 Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Lipid disorders are related to the development of cardiovascular and cerebrovascular diseases and therefore is considered an important risk factor that can be treated by drugs or even with changes in eating habits and physical. The result is a tucum?? regional physico-chemical features important for the treatment of dyslipidemia, such as substantial concentration of fibers. Thus, the present study aims to conduct in vivo study in rats untrained and trained on the use of tucum?? the Amazons in the treatment of diet-induced dyslipidemia. Was performed for both the induction of dyslipidemia followed by treatment with a base ration tucum?? with or without swimming. We used 40 male rats weighing about 350 grams. These animals were divided into 4 groups of 10 animals forming the Sedentary Control Group (SCG) the exercised control group (GCE), the Group Treatment Sedentary (GTS) and Treatment Group exercised (GTE). It is the body mass gain, feed intake, plasma concentrations of cholesterol, triglycerides, HDL-C, LDL-C, VLDL, total protein, and the hormones insulin and leptin. The GCE group showed a tendency to higher food consumption and the groups that consumed the flesh of the fruit tucum?? showed a trend to a higher body mass gain. The descriptive analysis showed that the groups that consumed the diet increased the fruit tucum?? showed a trend to a higher body mass gain. The GCE has a tendency to higher plasma cholesterol, as well as the GTE GTS and have higher absolute values for triglycerides and VLDL-C HDL lipids and LDL-C were shown to be elevated in the group that consumed tucum??. Through research it can be seen that the fruit tucum?? (Meyer Astrocatyum aculeatum) hypolipidemic has no effect, since the animals had elevated concentrations of lipid As dislipidemias est??o relacionadas ao desenvolvimento de doen??as cardiovasculares e cerebrovasculares e, portanto, ?? considerado um fator de risco importante que pode ser tratado atrav??s de f??rmacos ou mesmo com mudan??as nos h??bitos alimentares e f??sicos. O tucum?? ?? um fruto regional que apresenta caracter??sticas f??sico-qu??micas importantes para o tratamento das dislipidemias, como a concentra????o consider??vel de fibras. Diante disso, o presente estudo tem como objetivo realizar o estudo experimental in vivo em ratos Wistar sedent??rios e exercitados sobre o consumo de tucum?? do amazonas no tratamento da dislipidemia induzida por dieta. Para tanto foi realizado a indu????o da dislipidemia seguido do tratamento com ra????o a base de tucum?? associado ou n??o a nata????o. Foram utilizados 40 ratos machos pesando em m??dia 350 gramas. Esses animais foram divididos em 4 grupos com 10 animais formando o Grupo Controle Sedent??rio (GCS) o Grupo Controle Exercitado (GCE), o Grupo Tratamento Sedent??rio (GTS) e o Grupo Tratamento Exercitado (GTE). Verificou-se o ganho de massa corporal, consumo de ra????o, a concentra????o plasm??tica de colesterol, triglicer??deos, HDL-c, LDL-c, VLDL, prote??nas totais, al??m dos horm??nios insulina e leptina. O grupo GCE apresentou tend??ncia ao maior consumo alimentar e os grupos que consumiram a polpa do fruto tucum?? apresentaram uma tend??ncia ao maior ganho de massa corporal. A an??lise descritiva demonstrou que os grupos que consumiram a dieta acrescida do fruto tucum?? apresentaram uma tend??ncia ao maior ganho de massa corporal. O grupo GCE apresenta uma propens??o a maior concentra????o plasm??tica do colesterol, assim como os GTS e GTE apresentam maiores valores absolutos para os triglicer??deos e para o VLDL Os lip??dios HDL-c e LDL-c mostraram-se elevados nos grupos que consumiram tucum??. Atrav??s da pesquisa realizada pode-se observar que o fruto tucum?? (Astrocatyum aculeatum Meyer) n??o apresenta efeito hipolipid??mico, visto que os animais apresentaram concentra????es elevadas dos lip??deos