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The National Internal Areas Strategy has been active in Italy for some years. It aims at contrasting the depopulation of peripheral areas of our country and affirming a way of making public policies that takes into account the singular multiplicity of territorial situations that characterize it. It also represents a battle aimed at a more socially and territorially equitable development. This contribution proposes reflections starting from the case study of the Municipality of Palomonte (SA), developed within the second level Master in Sustainable Maintenance and Rehabilitation of the built environment of the University of Naples Federico II. Palomonte is an "intermediate" area, according to the classification proposed by the SNAI, which, with a demographic trend peculiar to the internal areas of Southern Italy, in recent years is affected by processes of depopulation and fragmentation of the territory. Through the combination of a place-based approach with the capability approach, and on the basis of a system vision between poles and internal areas, the research proposes short to medium term adaptive reuse scenarios, with the aim of triggering a reversal of the current demographic trend, attracting old and new inhabitants to invest in the area.

We propose the construction of LEGEND-1000, the ton-scale Large Enriched Germanium Experiment for Neutrinoless $\beta \beta$ Decay. This international experiment is designed to answer one of the highest priority questions in fundamental physics. It consists of 1000 kg of Ge detectors enriched to more than 90% in the $^{76}$Ge isotope operated in a liquid argon active shield at a deep underground laboratory. By combining the lowest background levels with the best energy resolution in the field, LEGEND-1000 will perform a quasi-background-free search and can make an unambiguous discovery of neutrinoless double-beta decay with just a handful of counts at the decay $Q$ value. The experiment is designed to probe this decay with a 99.7%-CL discovery sensitivity in the $^{76}$Ge half-life of $1.3\times10^{28}$ years, corresponding to an effective Majorana mass upper limit in the range of 9-21 meV, to cover the inverted-ordering neutrino mass scale with 10 yr of live time.

El volumen objeto de esta reseña viene avalado, entre otras cosas, por el Consejo Europeo de Investigación, quien otorgó su apoyo para la realización de esta edición. En él, se recopilan 23 relaciones mexicanas elaboradas entre 1579 y 1582, documentos todos ellos originales, en los que intervinieron escribientes indígenas, criollos y españoles. La precisión y el rigor en el aporte detallado de los intervinientes preside cada una de estas relaciones, que se agrupan según las diferentes áreas y las distintas autorías e intervinientes, ya que en el proceso de redacción de estos singulares documentos intervenían, según demuestra la investigadora, diversos ejecutores. Así, por ejemplo, para el área de Zimapán...

Communication (Communication avec actes dans un congrès); International audience; This study had two aims. The first was to improve the regeneration efficiency of Pelargonium leaf discs by adventitious budding. The second was to test the effect of gibberellic acid (GA 3) and paclobutrazol (PBZ) on callus formation and adventitious shoot regeneration in Pelargonium before using genetic transformation of this species for functional validation of genes involved in the process of GA regulation. GA 3 and paclobutrazol (an inhibitor of GA synthesis pathway) were added (together or separately) in the shoot regeneration media of two Pelargonium species, Pelargonium * hortorum 'Panache sud' ('P.sud') and Pelargonium * domesticum 'Autumn haze' (' P.dom'). In both cases, GA 3 applied alone, completely inhibited the bud regeneration. Moreover, the rate of callus formation decreased drastically when 5 M of GA 3 was applied to ' P. dom' explants. Similar result was obtained with 'P.sud' explants using 20 M GA 3. Paclobutrazol (0.3 M) applied at the same time as GA 3 (10 M) could partially restore regeneration process of ' P. dom'. For ' P. dom', the use of paclobutrazol alone increased callus formation and slightly improved the rate of regeneration. Moreover, initiated buds had a better appearance. For 'P. sud', which had an abundant callusing, paclobutrazol did not improve regeneration and led to hyperhydric shoots.

Suspension cell cultures of Helianthus annuus L. were previously established for the production of the most active component of vitamin E, alpha-tocopherol, by optimizing medium composition and culture conditions. In the present work, the possibility of enhancing alpha-tocopherol production by the addition of jasmonic acid to the culture medium was investigated both in sunflower and Arabidopsis cell cultures. A considerable increase (49% and 66%, respectively) of alpha-tocopherol production was obtained in both, after a 72-h treatment with 5 microM jasmonic acid. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

In the last years many studies on anthocyanins, the flavonoids responsible for the orange, red and blue colors in different plant organs, have revealed their antioxidant activity and a possible use as chemotherapeutics. The finding that tart cherries (Prunus cerasus L.) contain a great amount of anthocyanins, constituents that possess strong antioxidant and anti-inflammatory activities, have attracted much attention. Here, we report the preliminary results of the induction of anthocyanin biosynthesis in sour cherry callus cell cultures and the characterization of those callus cultures for cell growth and anthocyanin production. Callus cultures have been obtained from the leaf of in vitro grown shoots of two sour cherry cultivars, Amarena Mattarello (A.M.) and Stevnsbaer (S.). Several in vitro culture conditions have been tested to induce the anthocyanin biosynthesis using different media with reduced nitrogen and elevated sucrose contents in order to stimulate anthocyanin accumulation. No stimulatory affect from elevated sucrose concentration was observed, while reduced nitrogen and light seems to be the triggering factors. The pigment production is absent during the first days of growth at the light; afterwards, the pigment accumulation is stimulated by elicitors as light and nutritional factors, and reach a maximum nearly the 20th day of culture, during the exponential phase of growth. The in vitro pigment production system described in cherry callus cultures has given these preliminary but encouraging results, making the plant material very suitable and plastic to the in vitro manipulations.