Search

Objetivou-se investigar a percepção dos beneficiários quilombolas, graduados/as e graduandos/as, da comunidade quilombola da Serra do Evaristo, Baturité, Ceará, sobre a política pública de acesso ao ensino superior. Estudo de caso descritivo-exploratório, qualiquantitativo, cumprindo as questões éticas exigidas. Os resultados encontrados indicam: satisfação com o curso escolhido; acolhimento pela comunidade acadêmica; aplicam os conhecimentos obtidos e as habilidades desenvolvidas na academia em suas atividades na comunidade; atribuem relevância ao ensino superior em termos de ascensão social, melhoria da renda e maior inserção no mercado de trabalho. Os sujeitos da pesquisa sugerem maiores ofertas de vagas para os quilombolas; nas produções científicas; nos grupos de pesquisa; nos projetos na área de saúde, formação na área ambiental e sobre o legado cultural afro-brasileiro e africano.

Este estudo teve como objetivo avaliar a qualidade microbiológica de amostras de queijo Minas frescal comercializados na feira livre do município de Pedro Leopoldo, Minas Gerais. Foram coletadas sete amostras de queijo Minas frescal que foram avaliadas quanto a contagem de bactérias do grupo coliformes a 45ºC (termotolerantes), Staphylococcus coagulase positiva e pesquisa de Salmonella spp. Em 43% das amostras foram encontradas contagens de bactérias do grupo coliformes a 45ºC acima dos valores permitidos pela legislação brasileira vigente. Todas as amostras analisadas apresentaram contagens de Staphylococcus coagulase positiva fora dos padrões legais. Nenhuma amostra apresentou contaminação por Salmonella spp. Observou-se que todas as amostras de queijo analisadas encontravam-se impróprias para o consumo, pois apresentaram contagens acima do limite permitido pela legislação sanitária para pelo menos um dos indicadores avaliados. Os queijos analisados são considerados potencialmente capazes de causar infecções e/ou intoxicações alimentares aos consumidores. O consumo de alimentos que apresentam qualidade microbiológica insatisfatória é um problema de saúde pública.

Consagrado como o primeiro colégio de ensino secundário da região de Feira de Santana, o ―Gymnasio Santanópolis‖ foi uma instituição escolar de referência desde sua fundação na década de 1930, até o ano de 1985.

BACKGROUND/AIMS: Pre-mRNA splicing is an essential step in eukaryotic gene expression regulation. Genes are composed of exons that remain in the mature mRNAs and intervening sequences named introns. Splicing is the removal of introns and ligation of exons in a mature transcript. Splice site or spliceosome component mutations can lead to different diseases, including neurodegenerative diseases and several cancer types. HuR is an RNA-binding protein that preferentially binds to U- and AU-rich elements, usually found at the 3' UTRs of some mRNAs. We previously observed HuR specifically associated with spliceosomes assembled on introns containing miR-18a and miR-19a. miR-18a and miR-19a are components of the intronic miR-17-92 cluster, along with other five miRNAs. This cluster has been reported to regulate proliferation, migration, and angiogenesis in cells. In this context, we reasoned HuR could be controlling the splicing and processing of these miRNAs, leading to altered cellular phenotypes. METHODS: We induced HuR overexpression in BCPAP and HEK-293T and analyzed the expression of miRNAs using qPCR, as well as the phenotypic effects in those cells. Cell counting to analyze cell growth was performed after trypan blue staining. Migration and invasion assays were performed using transwell filters and cells were counted after staining with crystal violet. We knocked down HuR using a specific siRNA and analyzed expression of miRNAs by qPCR, as well as cellular kinetics. RESULTS: Our results revealed HuR is associated with miR-19a in BCPAP and HEK-293T cells. Conversely, silencing HuR led to reduced miR-17-5p and miR-19a in BCPAP cells. Our data support that HuR stimulates the expression of miR-19, which is further processed and capable of finding its target sequence in a reporter plasmid. Cells overexpressing HuR showed increased cellular proliferation, migration, and invasion rates. Notably, under the presence of antimiR-19a, BCPAP-HuR cells showed reduced cell growth. Taken together, these results indicate the molecular alterations observed are associated with upregulation of miR-19a, leading to cellular processes involved in cancer development. CONCLUSION: Our findings propose a connection between HuR, miRNA biogenesis and cellular modifications. HuR stimulates miR-19a and miR-19b expression, which leads to up-regulation of cell proliferation, migration and invasion, promoting cancer development.

A produção corporativa das cidades brasileiras se dá sob condições de mercantilização do espaço urbano, associada à segregação socioespacial, especulação imobiliária e captura da dimensão coletiva e pública das cidades. Tal situação tem levado os moradores a acionarem estratégias para a defesa do direito à cidade, incluindo o sistema de justiça. O objetivo deste artigo é analisar a aplicabilidade e a eficácia de instrumentos jurídicos e urbanísticos na minimização dos impactos socioespaciais, tendo como estudo de caso o empreendimento Horto Bela Vista (HBV), no bairro do Cabula, na cidade de Salvador (BA). O estudo utiliza pesquisa bibliográfica e documental, particularmente sobre o processo constante no Ministério Público do Estado da Bahia. Como resultado, constatou-se que, apesar da importância das reparações e compensações conquistadas, os instrumentos acionados apresentam limites para sua efetividade, tensionados tanto na perspectiva do urbanismo corporativo quanto do direito à cidade. Corporate production of Brazilian cities takes place under commodification conditions of human space, associated with socio-spatial segregation, real estate speculation, and capture of the collective and public dimension of cities. In response, citizens have mobilized strategies to defend the right to the city, including the legal system. This paper sought to analyze the applicability and effectiveness of law and urban tools in minimizing socio-spatial impacts, using the Horto Bela Vista (HBV) project, at the Cabula neighborhood, Salvador, Bahia, as a case study. Data were collected by means of bibliographical and documental research, particularly on the case at the Public Prosecutor’s Office of the State of Bahia. Results show that, despite of the importance of the reparations and compensations achieved, the tools used present limited effectiveness, and are tensioned both from the perspective of corporate urbanism and the right to the city. La production corporative des villes brésiliennes se déroule dans des conditions de marchandisation de l’espace urbain, associées à la ségrégation socio-spatiale, à la spéculation immobilière et à la capture de la dimension collective et publique des villes. En réponse, les habitants ont mobilisé des stratégies pour défendre leur droit à la ville, y compris le système juridique. Cet article a cherché d’analyser l’applicabilité et l’efficacité du droit et des outils urbans pour minimiser les impacts socio-spatiaux, en utilisant le projet Horto Bela Vista (HBV), dans le quartier Cabula, à Salvador, Bahia, comme étude de cas. Les données ont été collectées par le biais des recherches bibliographiques et documentaires, notamment sur le procès auprès du ministère public de l’État de Bahia. Les résultats montrent que, malgré l’importance des compensations et des réparations obtenus, les outils utilisés présentent une efficacité limitée, et sont tendu aussi bien à partir de la perspective de l’urbanisme d’entreprise que de celle du droit à la ville.

O splicing é um processo crítico para expressão gênica em eucariotos, pois os genes são transcritos como pré-mRNAs, os quais são compostos por exons (sequências que permanecem nos mRNAs maduros) e introns (sequências intermediárias). O processo de splicing envolve a excisão de introns e a ligação de exons, resultando em transcritos maduros (mRNA). Este processo é realizado por uma maquinaria complexa chamada spliceossomo, que é composta por cinco pequenos RNAs associados a proteínas (snRNAs) e mais de 100 proteínas, sendo que algumas podem associar-se transitoriamente a componentes de spliceossomo ou aos pré-mRNAs. As hnRNPs são proteínas que podem associar-se ao complexo spliceossomo, desempenhando um papel regulador nos locais de splicing,/i> e frequentemente envolvidas na mediação de splicing alternativo. No genoma humano, mais de 70% dos miRNAs estão localizados em introns, portanto, é possível que o processo de splicing seja importante para sua maturação. O aumento da expressão do cluster de miRNAs miR-17-92 já foi relacionado ao desenvolvimento de muitas patologias, como câncer de tireoide, câncer de pulmão e linfoma. Estudos anteriores mostraram que a hnRNP A1 é importante para a maturação de do miR-18a. Neste trabalho nós investigamos a potencial associação das proteínas hnRNP A1 e hnRNP G aos miRNAs do cluster miR-17-92. Os resultados mostraram que a hnRNP A1 está associada a outros membros do cluster miR-17-92, e que a superexpressão dessa proteína aumenta as capacidades proliferativa, migratória e invasiva em células de câncer papilífero de tireóide, BCPAP. Splicing is a critical process for gene expression in eukaryotes, as genes are transcribed as pre-mRNAs, which are composed of exons (sequences that remain in mature mRNAs) and introns (intermediate sequences). The splicing process involves intron excision and junction of exons, resulting in mature transcripts (mRNA). This process is performed by a complex machinery called spliceosome, which is composed by five snRNAs and more than 100 proteins, some of which may transiently associate with spliceosome components or pre-mRNAs. HnRNPs are proteins that can associate with the spliceosome complex, playing a regulatory role at splicing sites and often involved in alternative splicing mediation. In the human genome, over 70% of miRNAs are located in introns, therefore, it is possible that the splicing process is important for their maturation. Increased miR-17-92 cluster expression levels have been related to the development of many pathologies, such as thyroid cancer, lung cancer and lymphoma. Previous studies have shown that hnRNP A1 is important for miR-18a maturation. We investigated the potential association of hnRNP A1 and hnRNP G proteins with miRNAs from the miR-17-92 cluster. The results showed that hnRNP A1 is associated with other members of the miR-17-92 cluster, and that overexpression of this protein increases the proliferative, migratory and invasive capabilities of papillary thyroid cancer cells, BCPAP.

The role of tumour microenvironment in neoplasm initiation and malignant evolution has been increasingly recognized. However, the bone marrow mesenchymal stromal cell (BMMSC) contribution to disease progression remains poorly explored. We previously reported that the expression of serine protease inhibitor kunitz‐type2 (SPINT2/HAI‐2), an inhibitor of hepatocyte growth factor (HGF) activation, is significantly lower in BMMSC from myelodysplastic syndromes (MDS) patients compared to healthy donors (HD). Thus, to investigate whether this loss of expression was due to SPINT2/HAI‐2 methylation, BMMSC from MDS and de novo acute myeloid leukaemia (de novo AML) patients were treated with 5‐Azacitidine (Aza), a DNA methyltransferase inhibitor. In MDS‐ and de novo AML‐BMMSC, Aza treatment resulted in a pronounced SPINT2/HAI‐2 levels up‐regulation. Moreover, Aza treatment of HD‐BMMSC did not improve SPINT2/HAI‐2 levels. To understand the role of SPINT2/HAI‐2 down‐regulation in BMMSC physiology, SPINT2/HAI‐2 expression was inhibited by lentivirus. SPINT2 underexpression resulted in an increased production of HGF by HS‐5 stromal cells and improved survival of CD34+ de novo AML cells. We also observed an increased adhesion of de novo AML hematopoietic cells to SPINT2/HAI‐2 silenced cells. Interestingly, BMMSC isolated from MDS and de novo AML patients had increased expression of the integrins CD49b, CD49d, and CD49e. Thus, SPINT2/HAI‐2 may contribute to functional and morphological abnormalities of the microenvironment niche and to stem/progenitor cancer cell progression. Hence, down‐regulation in SPINT2/HAI‐2 gene expression, due to methylation in MDS‐BMMSC and de novo AML‐BMMSC, provides novel insights into the pathogenic role of the leukemic bone marrow microenvironment.

O calcio e um mineral proveniente principalmente do grupo de leite e derivados de extrema importância para o desenvolvimento adequado da crianca. Diversos estudos observaram uma diminuicao no consumo desses alimentos devido a alteracoes no padrao alimentar da populacao, o que pode levar a um crescimento inadequado, problemas nos dentes e nos ossos, e alguns estudos recentes tem evidenciado tambem associacao desta reducao com a ocorrencia da obesidade. O presente estudo visa avaliar o consumo de leite e derivados e o estado nutricional de criancas do municipio de Barretos, Sao Paulo. Foi realizado um estudo transversal com 81 criancas de 5 a 10 anos, e o consumo alimentar foi avaliado utilizando um recordatorio de 24 horas (R24h) e foram feitas afericoes antropometricas. A media de consumo de calcio entre as criancas foi de 771,72 mg, estando inadequado em 70,4% da amostra. O consumo de porcoes de leites e derivados foi inadequado em 76,5% das criancas havendo diferenca significativa entre os sexos, meninos apresentaram um maior consumo (p= 0,021). Foi observada uma relacao positiva entre o consumo de calorias totais e o consumo dos leites e derivados (p=0,011). Acerca da classificacao do estado nutricional, 32,1% das criancas apresentaram sobrepeso/obesidade. Nao foi encontrada associacao significativa entre o consumo de leite e derivados e calcio e estado nutricional das criancas.

Os fatores de risco para o desenvolvimento da hipertensao arterial incluem obesidade, tabagismo, sedentarismo e historico familiar. Esta ocorre principalmente devido ao aumento da atividade simpatica do sistema nervoso autonomico. Desta forma, o objetivo deste estudo foi avaliar as respostas hemodinâmicas e autonomicas em filhos de pais hipertensos apos uma sessao de treinamento da musculatura inspiratoria (TMI) ou Sham (ficticia) frente a um teste de estresse mental. Foram selecionados 14 jovens com idade entre 18 e 30 anos, submetidos a uma sessao de 15min de TMI em intensidade moderada (30% PImax) e a sessao Sham, de 15min sem resistencia ao fluxo respiratorio. Para a prescricao da intensidade de treinamento foi realizada a avaliacao da pressao inspiratoria maxima (PImax) pelo manovacuometro. Para a analise da modulacao autonomica foi realizado o registro batimento a batimento por fotoplestimografia antes, durante e apos a aplicacao do teste de estresse mental. Em relacao a modulacao autonomica, o balanco simpato-vagal nao apresentou diferenca apos a sessao Sham vs TMI. O mesmo foi observado em relacao as bandas de baixa (BF) e alta frequencia, que representam a modulacao simpatica e parassimpatica, respectivamente. Os dados sugerem que houve aumento na variabilidade da pressao arterial sistolica (PAS) e no BF-PAS durante o teste de estresse mental, no entanto, uma unica sessao de TMI nao foi suficiente para melhorar a modulacao autonomica dos filhos de hipertensos.

Esta pesquisa tem o objetivo de propor a construção de um glossário bilíngue (inglêsportuguês) de colocações especializadas com a palavra acorde, relacionadas à área de Harmonia Musical. Ela se justifica pela falta de estudos terminológicos relacionados às colocações especializadas do campo da Teoria Musical. Os alicerces teóricos que sustentam a pesquisa são: os Estudos da Tradução Baseados em corpus, que vincula a metodologia da Linguística de Corpus (LC) aos Estudos Descritivos da Tradução; a Teoria Comunicativa da Terminologia (TCT) que orienta a análise terminológica e a elaboração de glossários especializados; e a Fraseologia, que fornece respaldo à análise das colocações. Para este estudo foi construído um corpus comparável de textos escritos em Inglês (EUA) e Português (BR), com publicações ou reedições a partir de 1990, abrangendo os seguintes gêneros: instrucionais (livros-textos, apostilas) e acadêmico-científicos (artigos científicos, dissertações e teses). Os materiais, coletados de meio eletrônico e impresso, provém de sites de revistas especializadas, periódicos, congressos, conservatórios e faculdades de Música. A análise descritiva do corpus de estudo conta com a ajuda do software Wordsmith Tools (Scott, 2016) que gera listas de palavras-chave, colocados, clusters e concordâncias. A partir da análise das listas e comparação entre contextos nas duas línguas, foi elaborado um glossário, cujos verbetes estão organizados em ordem alfabética, com exemplos de uso retirados do corpus. O produto final tem, como público alvo, tradutores e músicos. This research aims to propose the construction of a bilingual English-Portuguese glossary of specialized collocations related to the term chord from the area of Musical Harmony. The reason for this study is the lack of terminological researches about specialized collocations from Musical Theory. This research is based on the following theoretical foundations: Corpus-based Translation Studies that links the methodology of Corpus Linguistics (CL) to Descriptive Translation Studies; The Communicative Theory of Terminology (TCT) which guides the terminological analysis and the elaboration of specialized glossaries; and Phraseology, which gives support to the analysis of collocations. A comparable corpus of texts written in English (USA) and Portuguese (BR) was compiled for this study. The texts published from 1990 are subdivided into two text genres: instructional texts (textbooks, study manuals) and scientific research (scientific articles, dissertations and theses). These materials, collected from electronic and print media, were obtained from the websites of specialized magazines, periodicals, conferences, conservatories and music colleges. The descriptive analysis of the study corpus was accomplished from the data provided by the software Wordsmith Tools (SCOTT, 2016) that generates wordlists, keywords, clusters and concordances. From the analysis of the lists and the comparison between contexts in both languages, we elaborated a glossary, whose entries are organized alphabetically, with examples of usage extracted from the corpus. The final product is intended to assist translators and musicians.

Background: The idea of studying bone marrow outside its native environment is attractive and ideal. Due to the many functions of extracellular matrix (ECM), currently there is an interest in creating an environment that mimics the ECM present in the tissue, similar to the microenvironment in vivo. Molds replacing the ECM (scaffolds) have a porous structure and may assist the tissue regeneration by forming a suitable environment for adhesion, migration, proliferation and cellular differentiation. The appropriate ECM is a key factor as ECM proteins are site-specific and provide protein 'footprints' of previous resident cells. Because ECM proteins are among the most conserved proteins, the removal of xenogenic/allogenic cellular contents via decellularization could theoretically produce an essentially minimally immunogenic scaffold with a native intact structure for new tissue regeneration. Thus, the search for a scaffold that could be used to assess the behavior of cells and their interactions with the ECM in vitro/in vivo, and has different niches in its composition is highly desirable. Aims: In recent years, a large number of molecular and cytogenetic abnormalities have been identified in AML, MDS and multiple myeloma, many of these defects can serve as markers for diagnosis/prognosis or as therapeutic targets. However, there are still many unknown molecular factors involved in genetic abnormalities or signaling pathways that contribute to the pathogenesis of the disease. Another very important aspect of these diseases is that they all are related to the mutual interaction of neoplastic cells and the microenvironment of bone marrow. In the absence of an ideal model or even the difficulty in reproduce a native environment, we proposed the characterization of a natural scaffold, from bovine bone marrow, which can be used as a study model, previously patented by our laboratory. Materials and Methods: Bone marrow was decellularized by one or more incubations in an enzymatic digestion solution and polar solvent extractions, comprising an extracellular matrix with well-preserved 3D structure. Scaffolds were analyzed after the decelularization process for potential changes in structure (TEM, SEM, Histological staining, and immunohistochemistry for collagen III, IV, fibronectin) and mechanical properties. To verify if the scaffold would hold and support cell survival and extracellular matrix production, an in vitro study was performed using CD34+ (non-stromal) and HS-5 (stromal) cells. Cell-seeded decellularized scaffolds were cultured for 7-14 days and analyzed for Histological staining. Results: Histology sections (H&E staining), TEM and SEM demonstrated the structure and ultrastructure of the processed matrix and confirmed both cellular extraction and preservation of the macroscopic 3-D architecture of the collagen fibers, blood vessels, and preservation of an organized matrix. Also, the decellularized scaffold was quite comparable to the native tissue in terms of its mechanical properties. Immunohistochemistry of the scaffold showed that the main components of the ECM were preserved. The in vitro experiments of both stromal cells (HS-5) and non-stromal cells (CD34+) demonstrated that they were able to adhere and in the HS-5 case also produce ECM during 7-14 days of culture. In both cases, an increase in cell number was observed and CD34+ overtime formed cluster and with 14 days of culture the cluster formation increased in size. Conclusions: The results demonstrated that the decellularization process was efficient in keeping a 3-D structure and mechanical properties with a well-organized-preserved ECM. In vitro experiments showed that both CD34+ and HS-5 were able to proliferate and adhere in specific sites of the scaffold, suggesting that they were able to recognize their native environment. HS-5 produced ECM indicating that the scaffold worked as an optimal microenvironment. In conclusion, the scaffold could be used as a model, which has the potential to mimic the native microenvironment to enable research/studies of factors that are involved in self-renewal and maintenance of neoplastic cells in bone marrow. Also, this model could be very useful for pharmacological testing of bone marrow in vitro. Disclosures No relevant conflicts of interest to declare.