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Con el texto que hoy se presenta, denominado PROCEDIMIENTOS, MANIOBRAS Y TÉCNICAS QUIRÚRGICAS EN URGENCIAS, se pretende hacer una contribución real a nivel teórico como herramienta de enseñanza – aprendizaje que discurren estrictamente sobre contenidos específicos que contribuyen al conocimiento y comprensión de diversas técnicas quirúrgicas, pruebas de diagnóstico y maniobras en la atención del parto en presentación pelviana. No se trata de un tratado sobre todas las técnicas quirúrgicas, por lo cual, es posible que existan facetas que se escapen o se queden cortas. La información podría ser interminable debido a la vastedad existente de la misma, por ello, se insta a los usuarios a continuar leyendo, estudiando, aprisionando información disponible en textos, investigaciones y artículos científicos, tesis de grado, etc., la cual les permita nutrir cada día más el conocimiento en tan importante área de la medicina y específicamente de la Cirugía General. Las técnicas quirúrgicas son los procedimientos de manipulación mecánica de las estructuras anatómicas con un fin médico, bien sea diagnóstico, terapéutico o pronóstico. Este libro está enfocado básicamente al conocimiento sobre algunos procedimientos diagnósticos, maniobra obstétrica y técnicas quirúrgicas, el cual está dirigido a estudiantes y profesionales de la salud.

Meloidogyne incognita, one of the most important root-knot nematodes of crops, is still mainly controlled with synthetic agrochemicals. Toward finding eco-friendly alternative controls such as bioc...

Antecedentes y Objetivos: Los micromicetos asexuales (MA) constituyen un grupo diverso y abundante de hongos en la naturaleza. Hasta la fecha, para el Estado de México no se habían realizado estudios sobre estos hongos. El objetivo de esta aportación es proporcionar información sobre la riqueza taxonómica de las especies de MA que habitan en hojas vivas senescentes y muertas de cinco especies de Quercus de un bosque templado en el municipio Huixquilucan, Estado de México, México.Métodos: Durante un año, quincenalmente se cortaron hojas de cinco especies diferentes de encinos vivas senescentes y se recolectaron hojas del suelo. Las muestras se incubaron en cámaras húmedas; la revisión microscópica se efectuó dos veces por semana. La determinación taxonómica se basó en la conidiogénesis y en las características morfométricas de los conidiomas. El material de referencia (preparaciones microscópicas), se depositó en el herbario del Instituto de Ecología, A.C. (XAL) en Xalapa, Veracruz, México. Los nuevos registros para la micobiota de México se ilustran y describen en detalle.Resultados clave: Se identificaron 30 especies de MA, 12 de ellas constituyen nuevos registros para el país y todas lo son para el Estado de México. En las hojas muertas del suelo hubo un mayor número de especies que en las hojas vivas senescentes. Ocho de los taxones identificados no habían sido recolectados desde que fueron descritos como especies nuevas para la ciencia. Trichothecium roseum, Pestalotiopsis maculans y Vermiculariopsiella immersa son micromicetos que están ampliamente distribuidos en las hojas de todas las especies de encinos estudiadas.Conclusiones: La riqueza de MA encontrada en el área de estudio evidencia que las hojas de los Quercus son sustratos que albergan una considerable riqueza fúngica aun desconocida en México. Lo anterior es una importante razón para la conservación de los bosques de Quercus y promover estudios sobre este grupo de micromicetos en nuestro país.

Background: Saprophytic micromycetes are recognized for their extraordinary ability to biosynthesize various metabolites with biological properties that can be modified when interacting with other organisms. Objective: To evaluate the antibacerial and antifungal activity of extracts obtained from dual interactions of Acremonium masseei CICY029 (Am), Beltraniella portoricensis MR42 (Bp), Cylindrium elongatum MR45 (Ce) y Stachybotrys sp. MR33 (Ssp) and to obtain the chemical profile of the most active interaction. Methodology: The ethyl acetate extracts of the four strains and their six dual interactions were evaluated against ten pathogens by the microdilution method to determine their antifungal and antibacterial capacity. The most active extracts were partitioned, and the minimum inhibitory concentration (MIC) o their fractions were determined. The chemical profile of the most active interaction was analyzed by gas chromatography coupled to mass spectrometry. Results: The highest mycelial growth inhibitory activity (MCI = 100%) was obtained from the fungal extracts of the single culture of Stachybotrys sp., and the dual Ce-Ssp interaction against Colletotrichum gloeosporioides, Mycosphaerella fijiensis at the concentration of 2000 µg/mL, as well as against Candida albicans and Xanthomonas campestris at 400 µg/mL. The acetonitrile fraction of Stachybotrys sp. showed high effectiveness against Alternaria tagetica, C. gloeosporioides, M. fijiensis and X. campestris (MIC ≤ 250 -1000 µg/mL). A mixture of fatty acids and three unidentified components not observed in the single cultures were detected by GC-MS in the Ce-Ssp extract. Implications: Biological interactions between two saprophytic fungi are a viable alternative for inducing the production of new metabolites with the potential to control pathogenic bacteria and fungi. Conclusions: The antifungal and antibacterial potential of saprophytic micromycetes can be improved in their dual interaction, particularly between C. elongatum MR45 and Stachybotrys sp. MR33, is a promising option for further investigation as potential natural antimicrobials.

The authors describe and illustrate new genus, Anasporidesmiella, typified by Sporidesmiella angustobasilaris [≡ A. angustobasilaris, comb. nov.], and a new species, A. manifesta . Anasporidesmiella is characterized by macronematous mononematous brown conidiophores frequently reduced to monoblastic determinate conidiogenous cells and solitary cylindrical distoseptate brown conidia with truncated or rounded bases. Anasporidesmiella manifesta is distinguished by cylindrical 7–12-distoseptate reddish-brown conidia with rounded basal cells. Several illustrations of conidiogenous cells and conidia of the Sporidesmiella species complement the discussion of the new genus.

This article provides an outline of the classification of the kingdom Fungi (including fossil fungi. i.e. dispersed spores, mycelia, sporophores, mycorrhizas). We treat 19 phyla of fungi. These are Aphelidiomycota, Ascomycota, Basidiobolomycota, Basidiomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Entorrhizomycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota. The placement of all fungal genera is provided at the class-, order- and family-level. The described number of species per genus is also given. Notes are provided of taxa for which recent changes or disagreements have been presented. Fungus-like taxa that were traditionally treated as fungi are also incorporated in this outline (i.e. Eumycetozoa, Dictyosteliomycetes, Ceratiomyxomycetes and Myxomycetes). Four new taxa are introduced: Amblyosporida ord. nov. Neopereziida ord. nov. and Ovavesiculida ord. nov. in Rozellomycota, and Protosporangiaceae fam. nov. in Dictyosteliomycetes. Two different classifications (in outline section and in discussion) are provided for Glomeromycota and Leotiomycetes based on recent studies. The phylogenetic reconstruction of a four-gene dataset (18S and 28S rRNA, RPB1, RPB2) of 433 taxa is presented, including all currently described orders of fungi., Nalin N. Wijayawardene thanks Mushroom Research Foundation and National Science Foundation of China (No. NSFC 31950410558) for financially supporting this project. Kevin D. Hyde acknowledges the Foreign Experts Bureau of Yunnan Province, Foreign Talents Program (2018; grant no. YNZ2018002), Thailand Research grants entitled Biodiversity, phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans (grant no: RSA5980068), the future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants, Rhododendron species and Dracaena species (grant no: DBG6080013), Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion (grant no: RDG6130001). H.T. Lumbsch thanks support by the Grainger Bioinformatics Center. E. Malosso is grateful to CAPES for financial support (grant no. 88881.062172/2014-01). B.T. Goto, G.A. Silva and K. Jobim, L.C. Maia acknowledges CNPq (Brazilian Scientific Council, grants no. 465.420/2014-1, 307.129/2015-2 and 408011/2016-5) and CAPES for support. The study was partially supported by the National Science Centre, Poland, under Grants No. 2015/17/D/NZ8/00778 and 2017/25/B/NZ8/00473 to Julia Pawłowska. The research of Martin Kukwa received support from the National Science Centre (NCN) in Poland (project no 2015/17/B/NZ8/02441). Alan J.L. Phillips acknowledges the support from UID/MULTI/04046/2019 Research Unit grant from FCT, Portugal to BioISI. H. Zhang is financially supported by the National Natural Science Foundation of China (Project ID: NSF 31500017). S. Boonmee would like to thank the Thailand Research Fund (Project No. TRG6180001). Dong-Qin Dai and Li-Zhou Tang would like to thank the National Natural Science Foundation of China (No. NSFC 31760013, NSFC 31260087, NSFC 31460561), the Scientific Research Foundation of Yunnan Provincial Department of Education (2017ZZX186) and the Thousand Talents Plan, Youth Project of Yunnan Provinces for support. R. Phookamsak, M. Doilom, D. N. Wanasinghe, S.C. Karunarathna and J.C. Xu express sincere appreciations to Key Research Program of Frontier Sciences of the Chinese Academy of Sciences (grant no. QYZDY-SSW-SMC014) for research financial support. R. Phookamsak thanks the Yunnan Provincial Department of Human Resources and Social Security (grant no. Y836181261), Chiang Mai University and National Science Foundation of China (NSFC) project code 31850410489 for research financial support. S.C. Kaunarathna thanks CAS President’s International Fellowship Initiative (PIFI) for funding his postdoctoral research (No. 2018PC0006) and the National Science Foundation of China (NSFC) for funding this work under the project code 31851110759. S. Tibpromma would like to thank the International Postdoctoral Exchange Fellowship Program (number Y9180822S1), CAS President’s International Fellowship Initiative (PIFI) (number 2020PC0009), China Postdoctoral Science Foundation and the Yunnan Human Resources, and Social Security Department Foundation for funding her postdoctoral research. Yuri S. Tokarev, Elena S. Nassonova and Irma V. Issi are indebtful to Yuliya Y. Sokolova (Institute of Cytology RAS, St. Petersbug, Russia) and Anastasia V. Simakova (Tomsk State University, Tomsk, Russia) for kind permission of reproduction of electron microscopy images of Metchnikovella incurvata and Crepidulospora beklemishevi, respectively. Yuri S. Tokarev and Irma V. Issi thank Russian Foundation of Basic Research, grant number 17-04-00871 (taxonomy of Rozellomycota). Elena S. Nassonova thank Russian Foundation of Basic Research, grant number 18-04-01359 (early evolution of Microsporidia, phylogeny of Metchnikovellida). Adam Flakus and Pamela Rodriguez-Flakus are greatly indebted to all staff of the Herbario Nacional de Bolivia, Instituto de Ecología, Universidad Mayor de San Andrés, La Paz and the SERNAP (http://sernap.gob.bo), for their generous cooperation providing permits, assistance and facilities support for scientific studies. The research of AF and PRF were financially supported by the National Science Centre (NCN) in Poland (DEC-2013/11/D/NZ8/03274). Adam Flakus and Pamela Rodriguez-Flakus received additional support under statutory funds from the W. Szafer Institute of Botany, Polish Academy of Sciences, Krakow, Poland. The authors would like to thank Yunnan Innovation Platform for Development and Utilization of Symbiotic Fungi Resources for finance support. Li-Fang Zhang would like to thank grant-in-aid from Science and Technology Department of Yunnan Province (2018FD080) for finance support. Chun-Ying Deng thanks the Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China (2019HJ2096001006). Yingqian Kang would like to thank Guizhou Scientific Plan Project [(2019) 2873]; Excellent Youth Talent Training Project of Guizhou Province [(2017) 5639]; Guiyang Science and Technology Project [(2017) No. 5-19]; Talent Base Project of Guizhou Province, China [FCJD2018-22]; Research Fund of Education Bureau of Guizhou Province, China [(2018) 481]. D. N. Wanasinghe would like to thank the CAS President’s International Fellowship Initiative (PIFI) for funding his postdoctoral research (number 2019PC0008), the National Science Foundation of China and the Chinese Academy of Sciences for financial support under the following grants: 41761144055, 41771063 and Y4ZK111B01. Yuri K. Novozhilov and Oleg N. Shchepin acknowledge support from the Russian Foundation of Basic Research, project 18-04-01232 А. Ivana Kušan, Neven Matočec, Armin Mešić and Zdenko Tkalčec are grateful to Croatian Science Foundation for their financial support under the project grant HRZZ-IP-2018-01-1736 (ForFungiDNA). K. Tanaka would like to thank the Japan Society for the Promotion of Science (JSPS 19K06802)

Natonodosa is introduced as new genus based on morphology and multigene phylogenetic data, and the monotypic genus Natonodosa, typified by N. speciosa, is characterized by mononematous conidiophores, polyblastic, nodose conidiogenous cells and unicellular, fusiform, hyaline conidia. The other freshly collected material was identified as Poroisariopsis inornata, a species that since its original description had not been reported; it is characterized by synnematous conidiophores with polytretic, cylindrical and uncinate conidiogenous cells with percurrent extensions, and distoseptate, obclavate, brown conidia. A lectotype and an epitype for the species are established and referred since the original specimen of this microfungus has not been found in any herbarium. Placement of both taxa in Xylariales was ascertained by BLASTn searches. Maximum likelihood analysis of the combined sequence data of the ribosomal rDNA (ITS and partial LSU region), as well as partial gene regions coding for the second largest subunit of RNA polymerase II (RPB2) and beta-tubulin (TUB2), was performed. In addition, a new name, Guarroa, is proposed for replacing the illegitimate genus name Phaeobotrys M. Calduch, Gene & Guarro. A dichotomous key to the new genus and morphologically similar taxa is provided.

Antecedentes y Objetivos: Los manglares proporcionan beneficios ecológicos y económicos. Además, albergan una alta diversidad de organismos dentro de los cuales están los hongos, que por su versatilidad proliferan tanto en restos vegetales como en la vegetación viviente en estos ecosistemas. No obstante su importancia, el conocimiento micológico en los manglares mexicanos es aún escaso. El objetivo central del presente trabajo es aportar información sobre la riqueza fúngica asociada a Rhizophora mangle, una de las especies arbóreas dominantes en los manglares del Caribe Mexicano.Métodos: Durante el año 2019 se recolectaron ejemplares fúngicos desarrollándose en ramas vivas y muertas, troncos caídos, raíces y hojarasca. Las colectas se efectuaron en manchones con poblaciones de R. mangle en la Reserva de la Biosfera Isla de Cozumel. La determinación taxonómica se llevó a cabo de acuerdo con las técnicas tradicionales en micología. Los ejemplares están depositados en el herbario de la Universidad Veracruzana (XALU) y en la colección de hongos de la Escuela Nacional de Ciencias Biológicas (ENCB).Resultados clave: Se determinarón 20 especies de hongos sobre diferentes estructuras de R. mangle. Se presentan 19 nuevos registros para la Isla de Cozumel, mientras que Arthonia conferta, Halorosellinia oceanica, Phyllosticta capitalensis, Pseudocamarosporium propinquum y Chaetosphaerella fusca son nuevos registros para México. La clase Arthoniomycetes presentó la mayor riqueza taxonómica. Los sustratos con mayor riqueza y abundancia fueron las cortezas de ramas. Conclusiones: La comunidad de hongos asociados a R. mangle esta dominada por ascomicetos. Los hábitos mejor representados son saprobios lignícolas y liquenizados epífitos.

Antecedentes y Objetivos: El género Mycoporum de la clase Dothideomycetes del phylum Ascomycota se caracteriza por un ascostroma con lóculos dispersos, agregados sin pared propia, ascas bitunicadas con un pedicelo corto y ascoporas elipsoidales, fusiformes a clavadas, con el hemisferio superior más ancho, y un numero variable de septos. El objetivo de este estudio es reportar a Mycoporum buckii por primera vez en México. Métodos: Los especímenes fueron recolectados sobre corteza de Rhizophora mangle en la Reserva de la Biosfera Isla de Cozumel, México, durante el año 2019, y fueron depositados en el herbario ENCB del Instituto Politécnico Nacional, México. El material se revisó en seco y se describieron sus características macro y micomorfologicas. Estos fueron identificados con claves y literatura espcializada. Resultados clave: Se presenta una descripción de Mycoporum buckii para México. Esta especie se caracteriza por un ascostroma multilocular, sésil, ascas clavadas y ascosporas elipsoidales con un septo transversal. Conclusiones: Mycoporum buckii se encontró creciendo sobre Rhizophora mangle y produce esporomas en temporada seca y húmeda en la Reserva de la Biosfera Isla de Cozumel, Quintana Roo, México. Palabras clave: Mar Caribe, Rhizophora mangle, taxonomía.

"Background and Aims: The genus Mycoporum of the class Dothideomycetes of the phylum Ascomycota is characterised by ascostromata with scattered, aggregated locules without a wall of their own, bitunicate asci with a short pedicel, as well as ellipsoidal, fusiform to clavate ascospores with broader upper hemispores, and a variable number of septa. The objective of this study is to report Mycoporum buckii for the first time in Mexico. Methods: The specimens were collected on Rhizophora mangle bark in the Cozumel Island Biosphere Reserve, Quintana Roo, Mexico, in 2019, and were deposited in the herbarium ENCB of the Instituto Politécnico Nacional, Mexico. The material was reviewed in dry state, and macro- and micromorphological characters were described. Specimens were identified with specialised keys and literature. Key results: The description of Mycoporum buckii of Mexico is presented. This species is characterised by multilocular, sessile ascostromata, clavate asci, and ellipsoidal ascospores with one transversal septum. Conclusions: Mycoporum buckii grows on Rhizophora mangle and produces its sporomas during the rainy and dry seasons in the Cozumel Island Biosphere Reserve, Quintana Roo, Mexico."

Micromycetes from unexplored sources represent an opportunity to discover novel natural products to control insect pests. With this aim, a strain of Acremonium masseei CICY026 isolated from a tropical sinkhole was identified, cultured on fermented rice, and its ethyl acetate extract (EAE) was evaluated against three serious phytophagous insects (Bemisia tabaci, Myzus persicae, and Rhopalosiphum padi). DNA from A. masseei CICY026 was used to confirm its identity. EAE caused settling inhibition (SI) of M. persicae and R. padi (67.5% and 75.3%, respectively). Bioassay-guided fractionation of the active EAE led to the isolation of a novel metabolite, named hexahydroacremonintriol (1), and of acremonin A glucoside (2). The structures of 1 and 2 were determined using IR, one- and two-dimensional NMR, HRMS, and confirmed by theoretical data. The aphid M. persicae was noticeably sensitive to 1 and 2 (SI: 55.6% and 67.2%, respectively), whereas R. padi was only slightly affected by 1 (SI: 59%). This new knowledge about mycobiota from these special sinkhole ecosystems will inform the development of new biorational pesticides.

Micromycetes from unexplored sources represent an opportunity to discover novel natural products to control insect pests. With this aim, a strain of Acremonium masseei CICY026 isolated from a tropical sinkhole was identified, cultured on fermented rice, and its ethyl acetate extract (EAE) was evaluated against three serious phytophagous insects (Bemisia tabaci, Myzus persicae, and Rhopalosiphum padi). DNA from A. masseei CICY026 was used to confirm its identity. EAE caused settling inhibition (SI) of M. persicae and R. padi (67.5% and 75.3%, respectively). Bioassay-guided fractionation of the active EAE led to the isolation of a novel metabolite, named hexahydroacremonintriol (1), and of acremonin A glucoside (2). The structures of 1 and 2 were determined using IR, one- and two-dimensional NMR, HRMS, and confirmed by theoretical data. The aphid M. persicae was noticeably sensitive to 1 and 2 (SI: 55.6% and 67.2%, respectively), whereas R. padi was only slightly affected by 1 (SI: 59%). This new knowledge about mycobiota from these special sinkhole ecosystems will inform the development of new biorational pesticides.

The effects of the interaction between arbuscular mycorrhizal and phosphate-solubilizing (P-solubilizing) fungi on phosphorous availability, acid phosphatase activity, and the growth and development of coffee plants (Coffea arabica L.) var. garnica were evaluated. The experiment was performed under controlled conditions and was based on a randomized factorial design with two factors. Coffee plants were inoculated with a consortium of arbuscular mycorrhizal fungi (CAMF), two strains of P-solubilizing fungi (PSF) (Aspergillus niger [An] and Penicillium brevicompactum [Pb]), the possible combinations of the latter fungi, and an uninoculated control. After 8 months, the results demonstrated the effectiveness of mycorrhizal and P-solubilizing fungal inoculations in increasing available soil phosphorous. The greatest concentration of available soil phosphorous was detected in the consortium of P-solubilizing fungi (CPSF) treatment at 3.8 mg/kg. The total foliar phosphorous concentration of plants was higher in the CAMF, An + CAMF, CPSF + CAMF, Pb + CAMF, and CPSF treatments in comparison to the control treatment. The growth of coffee plants was also favored by the consortium treatments (P-solubilizing fungi and arbuscular mycorrhizal fungi). The acid phosphatase activity in the rhizosphere significantly increased under the CPSF treatment and also increased in the roots of coffee plants under the An, An + CAMF, and CPSF + CAMF treatments. Given the importance of fungal groups for processes of phosphorous transformation and absorption in coffee plants, it is imperative to continue the search for native fungal strains with high potential for use as biofertilizers.

To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone. Our study demonstrated that co-culture with soil fungi might be a promising method for improving laccase production in F. floccosa. Although the enhancement of laccase activity was a function of P. commune inoculation time, two laccase isoenzymes produced by F. floccosa remained unchanged when strains were co-cultured. These data are compatible with the potential of F. floccosa in agricultural applications in soil, whose enzyme machinery could be activated by soil fungi such as P. commune., This study was supported by Spanish projects AGL2008-00572/AGR and Prolipapel II S-2009AMB-1480 as well as the Agencia Nacional de Promoción Científíca y Tecnológica (PICT 2015-1620 to Saparrat, M.).

Fungal diversity in the state of Veracruz, Mexico, is a source of enzymes with biotechnological value. The objective of this study was to isolate and evaluate qualitatively (in Petri dishes) and quantitatively (in liquid and solid fermentation) the ligninolytic enzyme activities of native macromycetes. Seventy isolates were made, and 41 showed laccase activity in the qualitative assessment. The best strains from the qualitative assessment were Trametes maxima SM9, Pycnoporus sanguineus ACT1 and Daedalea elegans PM7. These strains were chosen to evaluate the laccase and manganese peroxidase (MnP) activities in Bose, Koroljova and Sivakumar liquid culture media and for agroindustrial waste in wheat straw, sugar cane bagasse and pine sawdust. The highest laccase and MnP activities were obtained in Sivakumar culture medium. Trametes maxima showed the highest values for laccase (9121.8 U/ mg of protein) and MnP (477.9 U/mg of protein) activities, followed by P. sanguineus ACT1 (laccase = 5422.2 U/mg of protein and MnP = 41.0 U/mg of protein) and D. elegans PM7 (laccase = 1784.2 U/mg of protein and MnP = 40.0 U/mg of protein). In regards to the solid state fermentation, P. sanguineus ACT1 produced the highest laccase activity on wheat straw (1409.0 U/mg dry weight) and sugar cane bagasse (1404.8 U/mg dry weigh). Meanwhile, MnP activities were higher in sugar cane bagasse for P. sanguineus ACT1 and T. maxima SM9 with 619.0 and 519.6 U/mg dry weight, respectively. Trametes maxima SM9 and P. sanguineus ACT1 are considered good candidates for laccase and MnP production.

En la actualidad, es altamente necesario encontrar nuevosy mas seguros productos agroquimicos. En este sentido, los micromicetosson una fuente importante de productos naturales los cuales puedenser usados en el control de enfermedades vegetales. Con el objetivo decontribuir a la busqueda de productos con aplicaciones antimicrobianas,un total de 49 cepas fungicas fueron aisladas de dos cenotes de lapeninsula de Yucatan. Estas cepas se cultivaron en arroz fermentado, seobtuvieron sus respectivos extractos organicos macerados en acetato deetilo (EAE) y metanol (EM) y se evaluaron contra cinco fitopatogenosde importancia agricola. Estos patogenos incluyeron a los hongos Alternariachrysanthemi, Colletotrichum gloeosporioides, Mycosphaerella fijiensisy las bacterias Erwinia carotovora y Xanthomonas campestris, utilizandoel bioensayo en microdilucion. El 69% de los extractos fungicos evaluadosmostraron actividad antifungica y/o antibacteriana contra al menosuno de los patogenos evaluados a concentraciones de 2000 y 200 μg/mL, respectivamente. Las cepas de Penicillium sp. OSE-61, Fusariumsp. OH2-30, Hypocrea lixii OSN-37 y Rhizoctonia solani OSE-73mostraron actividad contra al menos tres de los fitopatogenos en estudio.Los extractos de EAE mas promisorios fueron particionados obteniendotres fracciones de baja (A), media (B) polaridad y un precipitado (C),los cuales fueron evaluados contra los patogenos estudiados. La fraccionA de Fusarium KS-15 fue la mas efectiva presentando la mas baja ConcentracionMinima Inhibitoria (MIC ≤25 μg/mL) y efecto bactericidacontra X. campestris. La fraccion B de Penicillium sp. OSE-61 contraA. chrysanthemi fue relativamente mayor (MIC ≤500 μg/mL); mientrasla fraccion B de H. lixii OSN-37 mostro efecto antifungico contra C.gloeosporioides y M. fijiensis (MIC = 1000 μg/mL). Esta investigacioncontribuye a enriquecer el conocimiento sobre la actividad biologica dehongos anamorficos nativos de los cenotes de Yucatan y su potencial usoen aplicaciones biotecnologicas en la agricultura.

Background: Cloud forest is one of the most threatened ecosystems in the world and more than half of its original area has been transformed for other land uses. Consequently, macro- and microorganisms of this ecosystem have likely undergone changes. Objective: To analyze the diversity of saprobic filamentous soil cultured fungi in four cloud forest fragments and evaluate the similarity in species composition among fragments. Methods: To detect and isolate soil microfungi, the soil particle filtration technique was used. Emerging colonies were transferred to a culture medium for subsequent quantification and identification. For each species, permanent and semi-permanent slide preparations were made, and taxonomic identification was performed based on morphological features. Results and conclusions: From 20 soil samples and 2000 inoculated particles, 233 species were distinguished. The most common species were Myrmecridium schulzeri, Pestalotiopsis maculans, Trichoderma atroviride, Virgaria nigra, Trichoderma cremeum, Fusarium solani, Cordana terrestris, Aspergillus fischeri, and Penicillium olsoni. The most abundant species were Talaromyces flavus and Pestalotiopsis maculans. All the forest fragments had a high richness, diversity, and evenness in addition to a high turnover and high number of exclusive species.

Knowledge of the relationships and thus the classification of fungi, has developed rapidly with increasingly widespread use of molecular techniques, over the past 10-15 years, and continues to accelerate. Several genera have been found to be polyphyletic, and their generic concepts have subsequently been emended. New names have thus been introduced for species which are phylogenetically distinct from the type species of particular genera. The ending of the separate naming of morphs of the same species in 2011, has also caused changes in fungal generic names. In order to facilitate access to all important changes, it was desirable to compile these in a single document. The present article provides a list of generic names of Ascomycota (approximately 6500 accepted names published to the end of 2016), including those which are lichen-forming. Notes and summaries of the changes since the last edition of ‘Ainsworth & Bisby’s Dictionary of the Fungi’ in 2008 are provided. The notes include the number of accepted species, classification, type species (with location of the type material), culture availability, life-styles, distribution, and selected publications that have appeared since 2008. This work is intended to provide the foundation for updating the ascomycete component of the “Without prejudice list of generic names of Fungi” published in 2013, which will be developed into a list of protected generic names. This will be subjected to the XIXth International Botanical Congress in Shenzhen in July 2017 agreeing to a modification in the rules relating to protected lists, and scrutiny by procedures determined by the Nomenclature Committee for Fungi (NCF). The previously invalidly published generic names Barriopsis, Collophora (as Collophorina), Cryomyces, Dematiopleospora, Heterospora (as Heterosporicola), Lithophila, Palmomyces (as Palmaria) and Saxomyces are validated, as are two previously invalid family names, Bartaliniaceae and Wiesneriomycetaceae. Four species of Lalaria, which were invalidly published are transferred to Taphrina and validated as new combinations. Catenomycopsis Tibell & Constant. is reduced under Chaenothecopsis Vain., while Dichomera Cooke is reduced under Botryosphaeria Ces. & De Not. (Art. 59)., Dayarathne would like to thank the Thailand Research Fund (TRF) grant no RSA5980068 entitled Biodiversity, phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans, National Research Council of Thailand (NRCT) entitled Diseases of mangrove trees and maintenance of good forestry practice (Grant number: 60201000201) and Mae Fah Luang University grant “Biodiversity, phylogeny and role of fungal endophytes of Pandanaceae” (Grant number: 592010200112). Hugo Madrid was funded by Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT), Chile, project no. 11140562.” Rafael F. Castañeda-Ruiz is grateful to the Organización Superior de Dirección Empresarial, Grupo Agrícola, (OSDE) from the Cuban Ministry of Agriculture and “Programa de Salud Animal y Vegetal”, project P131LH003033. Dong Qin Dai would like to thank the Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and plants on Yun-Gui Plateau for the support. Ka-Lai Pang thanks Ministry of Science and Technology, Taiwan for financial support (105-2621-B-019 -002-). Guo Zhu Zhao was funded by the National Natural Science Foundation of China (No. 31570019). Mingkwan Doilom acknowledges the Royal Golden Jubilee Ph.D. Program (PHD./0072/2553 in 4.S.M.F./53/A.2. K. Tanaka would like to thank the Japan Society for the Promotion of Science (JSPS; 26291084 and 16K07474). Walter P. Pfliegler was supported through the ÚNKP--16--4--IV New National Excellence Program of the Hungarian Ministry of Human Capacities. Samantha C. Karunarathna thanks Yunnan Provincial Department of Human Resources and Social Security funded postdoctoral project (number 179122) for supporting his postdoctoral research study. The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP#0089. KC Rajeshkumar thanks SERB, DST, Government of India for providing financial support under the project YSS/2015/001590 and Dr. K. M. Paknikar, Director, ARI for providing the facility. Mats Wedin thanks the Swedish Research Council, grants VR 621-2012-3990 and VR 2016-03589. Alan JL Phillips acknowledges the support from Biosystems and Integrative Sciences Institute (BioISI, FCT/UID/ Multi/04046/2013). L. Selbmann, L. Zucconi and S. Onofri thank the Italian National Program for Antarctic Researches (PNRA) for the financial support. The Italian National Antarctic Museum (MNA) is acknowledged for supporting the Mycological Section and the Culture Collection of Fungi from Extreme Environments (CCFEE).

We compared the abundance, species richness and diversity of saprobic filamentous microfungi in the forest and in coffee plantation systems (with different biophysical structures of the vegetation and agricultural management) and evaluated the degree of similarity in species composition among these sites. Soil washing was used to isolate the saprobic filamentous microfungi. Emerging colonies were quantified and transferred to tubes with culture medium and then mounted on semi-permanent slides for identification under the microscope. From 90 samples and 4,500 inoculated soils particles, 415 species were distinguished. The genera Trichoderma, Penicillium, Fusarium, Chaetomium and Humicola were the most frequent in all study sites. The transformation of the tropical montane cloud forest in coffee plantation had no significant effect on the abundance, species richness and diversity of the saprobic filamentous microfungi. Effects of the biophysical structures of the vegetation and the agricultural management of the sites were only detected at the level of dominant genera (Fusarium, Trichoderma and Penicillium) and on the evenness. The low degree of similarity among the six sites suggests the existence of a high exchange of species.

La riqueza fúngica de las áreas protegidas del estado de Tabasco ha sido pobremente estudiada. En su mayoría, las especies saprobias microscópicas aún se desconocen. La presente contribución tiene como objetivo documentar la composición de hongos anamorfos que proliferan en los restos vegetales de la selva del Parque Estatal “Agua Blanca”. En total se presentan 40 taxones, entre los cuales se incluyen 15 que no se conocían para México y 25 para el estado de Tabasco. De todos los registros nuevos a nivel nacional, junto con su descripción morfológica, se anexan ilustraciones, información sobre la distribución geográfica conocida y observaciones sobre sus caracteres de interés taxonómico.

Two new species in the genera Lobatopedis and Minimelanolocus were found during an investigation of anamorphic fungi from plant debris collected in a cloud forest in Veracruz State, Mexico. Both are described and illustrated. Lobatopedis kirkii sp. nov. is characterized by brown, macronematous conidiophores, monoblastic conidiogenous cells, and brown, cylindrical, narrowly obclavate to sub-acicular conidia which differ from those of other species of Lobatopedis in shape. Minimelanolocus limpidus sp. nov. is characterized by macronematous dark brown conidiophores, polyblastic conidiogenous cells with slightly melanized scars, and obclavate, (3–)6-septate, pale golden brown conidia. A comparative table and a key to Lobatopedis species are also provided. Key words : hyphomycetes, microfungi, nature conservation, taxonomy. Introduction Cloud forests (CF) are among the most diverse biomes in the world. In Mexico, this kind of forest, although covering less than 1% of the land surface, contains 10–12% of the country’s flowering plant species (Rzedowski 1996). Because of the climatological characteristics, all kinds of fungi grow in the wide variety of habitats provided by these forests. Unfortunately, CF are severely threatened not only in Mexico but also worldwide. Urgent exploration of the few remnants of such forests is therefore needed. As part of a long-term study of anamorphic fungi from cloud forests, multiple

Two microfungi collected from plant debris in cloud forests during a survey of the fungi associated with leaf litter in Veracruz are described and illustrated. Digitella rigidophora gen. et sp. nov. is distinguished by polytretic conidiogenous cells having a successive sympodial but rectilinear or geniculated proliferation and with discoid to papillate- perforate thickened conidiogenous loci and digitate solitary dark brown to brown conidia. Redbia inflata sp. nov. is characterized by alternate, curved to coiled, slightly echinulate branches that possess a distinct ampulla at the ends and by coarsely verruculose, subhyaline, 0-1-septate conidia.

In Mexico, the study of the composition and changes of the sporulating microfungi on decaying fallen leaves in rainforest ecosystems has not been elucidated. In this study, we evaluated the species richness, diversity, abundance and similarity of the sporulating microfungi inhabiting the leaf litter of Rinorea guatemalensis, a dominant evergreen tree in family Violaceae (Malphigiales) from the rainforest of the "Agua Blanca" park in the state of Tabasco. The study was done over a period of 210 days. In parallel we analyzed the loss of leaf biomass. The litter bag method was used. Fungal fruiting structures were detected and quantified using moist chambers. A total of 38 taxa were detected. Two main phases were detected in the biomass loss and in the changes in the microfungi community; the primary phase was characterized by the highest biomass loss and corresponded with the highest diversity, specific richness, as well the highest values of frequency of occurrence for the sporulating microfungi. In the secondary phase the biomass loss and diversity remain with little apparent changes and with a tendency of decrease gradually with increasing foliar decomposition. In the later stages of decomposition, the similarity in the microfungal community increased. The frequency and periodicity of occurrence values indicated that the sporulating microfungi community was structured mainly by rare and sporadic species. Cylindrocladium scoparium, Microthyrium sp., Volutella ciliata and Volutella minima were considered as residents throughout the study. fungal diversity / leaf litter / leaf decomposition / Mexico / tropical microfungi

El hemangioma es una neoplasia vascular benigna frecuente en la primera infancia con predilección en las mujeres, su localización es frecuente en la región cervicofacial en un 60% de los casos. Esta neoplasia representa un reto para el profesional de la salud puesto que la mayor complicación en estos pacientes son los problemas hemorrágicos u obstructivos a nivel de vías áreas El caso clínico que se presenta es un paciente masculino de 30 años con diagnóstico de hemangioma gigante derivado de músculo orbicular de labio tratado con cirugía parcial e interferon sin respuesta o contribución real, por lo cual, el paciente actualmente cursa con un aumento de volumen importante que causa deformidad en hemicara izquierda, rebasando línea media mandibular con las subsecuentes alteraciones bucales condicionadas y agravadas por la misma patología, lo cual repercute en su salud sistémica.The hemangioma is a benign vascular neoplasm frequently seen in early childhood, usually in females. Its location is common in the cervicofacial region in 60% of cases. This tumour represents a challenge for the health professional, as the major complications in these patients are bleeding disorders or obstructive airways. The clinical case presented is a 30 year old male patient with a diagnosis of a giant haemangioma arising from the orbicular muscle of the lip, treated with partial surgery and interferon, with no response or no real contribution. The volume of the haemangioma is currently increasing, causing a deformity on the left side of the face that exceeds the mandibular midline with subsequent oral alterations conditioned and aggravated by the disease itself, which is affecting his systemic health.

Arbuscular mycorrhizal fungi (AMF) are essential for growth of coffee plants in acidic and phosphate deficient soils in Central and South America. We investigated the species richness of Glomeromycotean fungi in 9–13 years old coffee plantations in Colombia and Mexico. Fungal species richness was related to physical and chemical soil factors, soil aggregate stability, and farm management practices. Using morphological spore characteristics 85 AMF species were found. AMF species similarity within the plantations was 62–73 %, and the most regularly AMF species found in both countries were: Acaulospora mellea, Acaulospora spinosa, Ambispora fennica, Diversispora aurantia, Dominikia aurea, Glomus brohultii, Rhizoglomus clarum and Rhizoglomus intraradices. In both countries species richness was differently influenced by agronomic management intensity and soil parameter. In Colombia, where soils were more fertile with higher organic matter and soil pH than in Mexico, soil parameter explained the variations in species diversity, while in Mexico, agronomic management intensities explained the variations in species richness among sites. Soil aggregate stability slightly explains the AMF species richness in Colombia and Mexico. It can be concluded that the Glomeromycotean species richness is very high in older coffee plantations in Colombia and Mexico, and that agronomic inputs have positive effects on species richness in very acidic soils with lower organic matter contents, while in more fertile soils like in Colombia, soil parameter define more the AMF richness.

This investigation was undertaken to determine the atrazine degradation by fungal enzyme extracts (FEEs) in a clay-loam soil microcosm contaminated at field application rate (5 μg g(-1)) and to study the influence of different soil microcosm conditions, including the effect of soil sterilization, water holding capacity, soil pH and type of FEEs used in atrazine degradation through a 2(4) factorial experimental design. The Trametes maxima-Paecilomyces carneus co-culture extract contained more laccase activity and hydrogen peroxide (H2O2) content (laccase = 18956.0 U mg protein(-1), H2O2 = 6.2 mg L(-1)) than the T. maxima monoculture extract (laccase = 12866.7 U mg protein(-1), H2O2 = 4.0 mg L(-1)). Both extracts were able to degrade atrazine at 100%; however, the T. maxima monoculture extract (0.32 h) achieved a lower half-degradation time than its co-culture with P. carneus (1.2 h). The FEE type (p = 0.03) and soil pH (p = 0.01) significantly affected atrazine degradation. The best degradation rate was achieved by the T. maxima monoculture extract in an acid soil (pH = 4.86). This study demonstrated that both the monoculture extracts of the native strain T. maxima and its co-culture with P. carneus can efficiently and quickly degrade atrazine in clay-loam soils.