1. Bacterial nanocellulose/calcium alginate hydrogel for the treatment of burns
- Author
-
Lauriene Luiza de Souza Munhoz, Luiz Carlos Guillens, Beatriz Candido Alves, Maria Gabriela Oliveira Ferreira do Nascimento, Andréia Bagliotti Meneguin, Fernanda Mansano Carbinatto, Gabriela Arruda, Hernane da Silva Barud, Andrea de Aro, Laura de Roch Casagrande, Paulo Cesar Lock Silveira, Thiago Antônio Moretti Andrade, Glaucia Maria Tech dos Santos, and Guilherme Ferreira Caetano
- Subjects
Alginates ,Anti-Inflammatory Agents ,Burns ,Cellulose ,Hydrogels ,Surgery ,RD1-811 - Abstract
ABSTRACT Purpose: Bacterial cellulose (BC) has shown high capacity for the treatment of wounds and burns, providing a moisty environment. Calcium alginate can be associated with BC to create gels that aid in wound debridement and contribute to appropriate wound healing. This study is aimed at characterizing and evaluating the use of bacterial cellulose/alginate gel in skin burns in rats. Methods: Cellulose and cellulose/alginate gels were compared regarding the capacity of liquid absorption, moisture, viscosity, and potential cytotoxicity. The 2nd degree burns were produced using an aluminum metal plate (2.0cm) at 120ºC for 20s on the back of rats. The animals were divided into non-treated, CMC(Carboxymethylcellulose), Cellulose(CMC with bacterial cellulose), and Cellulose/alginate(CMC with bacterial cellulose and alginate). The animals received topical treatment 3 times/week. Biochemical (MPO, NAG and oxidative stress), histomorphometry and immunohistochemical assays (IL-1β IL-10 and VEGF) were conducted on the 14th, 21st, 28th, and 35th days. Results: Cellulose/Alginate gel showed higher absorption capacity and viscosity compared to Cellulose gel, with no cytotoxic effects. Cellulose/alginate presented lower MPO values, a higher percentage of IL-10, with greater and balanced oxidative stress profile. Conclusions: The use of cellulose/alginate gel reduced neutrophils and macrophage activation and showed greater anti-inflammatory response, which can contribute to healing chronic wounds and burns.
- Published
- 2024
- Full Text
- View/download PDF