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187 results on '"Gaboriaud C"'

7. Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding

Author

Valentini, A., Taberlet, P., Miaud, C., Civade, R., Herder, J., Thomsen, P.F., Bellemain, E., Besnard, A., Coissac, E., Boyer, F., Gaboriaud, C., Jean, P., Poulet, N., Roset, N., Copp, G.H., Geniez, P., Pont, D., Argillier, C., Baudoin, J.M., Peroux, T., Crivelli, A.J., Olivier, A., Acqueberge, M., Le Brun, M., Moller, P.R., Willerslev, E., Dejean, T., Centre d'Ingénierie Hydraulique [Savoie Technolac] (CIH-EDF), EDF (EDF), Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Hydrosystèmes et Bioprocédés (UR HBAN), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), RAVON, Ravon, Centre for GeoGenetics, Natural History Museum of Denmark, Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)-Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Office national de l'eau et des milieux aquatiques (ONEMA), Ministère de l'écologie, du développement durable et de l'énergie, Centre for Environment, Fisheries and Aquaculture Science [Lowestoft] (CEFAS), Risques, Ecosystèmes, Vulnérabilité, Environnement, Résilience (RECOVER), Station Biologique de la Tour du Valat, Agence Centre-Ouest, Ecosphère, Laboratoire National d’Hydraulique et Environnement (EDF R&D LNHE), EDF R&D (EDF R&D), EDF (EDF)-EDF (EDF), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)-Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Hydrosystèmes et bioprocédés (UR HBAN)

Subjects
LAC, aquatic fauna, Monitoring, ADN, Environmental DNA, FAUNE AQUATIQUE, INVENTAIRE FAUNISTIQUE, dna, Amphibian, ICHTYOLOGIE, Fish, Wildlife management, ichthyology, lakes, Detection probability, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, animal population
Abstract

[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE; International audience; Global biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are important for efficient and timely management. Recent advances in DNA sequencing have provided a new tool for species detection from DNA present in the environment. In this study, we tested whether an environmental DNA (eDNA) metabarcoding approach, using water samples, can be used for addressing significant questions in ecology and conservation. Two key aquatic vertebrate groups were targeted: amphibians and bony fish. The reliability of this method was cautiously validated in silico, invitro and insitu. When compared with traditional surveys or historical data, eDNA metabarcoding showed a much better detection probability overall. For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI=0.90-0.99) vs. 0.58 (CI=0.50-0.63) for traditional surveys. For fish, in 89% of the studied sites, the number of taxa detected using the eDNA metabarcoding approach was higher or identical to the number detected using traditional methods. We argue that the proposed DNA-based approach has the potential to become the next-generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems. see also the Perspective by Hoffmann, Schubert and Calvignac-Spencer.

Published
2015
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13. Deciphering complement mechanisms: The contributions of structural biology

Author

Arlaud, G.J., Barlow, P.N., Gaboriaud, C., Gros, P., Narayana, S.V.L., Kristal- en structuurchemie, and Dep Scheikunde

Abstract

Since the resolution of the first three-dimensional structure of a complement component in 1980, considerable efforts have been put into the investigation of this system through structural biology techniques, resulting in about a hundred structures deposited in the Protein Data Bank by the beginning of 2007. By revealing its mechanisms at the atomic level, these approaches significantly improve our understanding of complement, opening the way to the rational design of specific inhibitors. This review is co-authored by some of the researchers currently involved in the structural biology of complement and its purpose is to illustrate, through representative examples, how X-ray crystallography and NMR techniques help us decipher the many sophisticated mechanisms that underlie complement functions.

Published
2007

14. L-ficolin complexed to sulphates

Author

Laffly, E., primary, Lacroix, M., additional, Martin, L., additional, Vassal-Stermann, E., additional, Thielens, N., additional, and Gaboriaud, C., additional

Published
2014
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24. M-ficolin mutant Y271F

Author

Gout, E., primary, Garlatti, V., additional, Smith, D.F., additional, Lacroix, M., additional, Dumestre-Perard, C., additional, Lunardi, T., additional, Arlaud, G.J., additional, Gaboriaud, C., additional, and Thielens, N.M., additional

Published
2009
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