1. Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers
- Author
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Gaëlle Chopin Stukart-Parsons, Arnold I. Caplan, D. Dozortsev, Lygia da Veiga Pereira, Humberto F. Cerruti, Alexandre Kerkis, Silvia Maria Gomes Massironi, and Irina Kerkis
- Subjects
Histology ,medicine.medical_treatment ,Cell Culture Techniques ,Oct-4 ,Biology ,Dental pulp stem cells ,medicine ,Humans ,Tooth, Deciduous ,Child ,Dental Pulp ,Stem cell transplantation for articular cartilage repair ,DNA Primers ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,Amniotic stem cells ,Cell Differentiation ,Stem-cell therapy ,Immunohistochemistry ,Cell biology ,Clone Cells ,Culture Media ,Amniotic epithelial cells ,Child, Preschool ,Karyotyping ,embryonic structures ,Anatomy ,Stem cell ,Octamer Transcription Factor-3 ,Biomarkers ,Cell Division ,Adult stem cell - Abstract
We report the isolation of a population of immature dental pulp stem cells (IDPSC), which express embryonic stem cell markers Oct-4, Nanog, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 as well as several other mesenchymal stem cell markers during at least 25 passages while maintaining the normal karyotype and the rate of expansion characteristic of stem cells. The expression of these markers was maintained in subclones obtained from these cells. Moreover, in vitrothese cells can be induced to undergo uniform differentiation into smooth and skeletal muscles, neurons, cartilage, and bone under chemically defined culture conditions. After in vivo transplantation of these cells into immunocompromised mice, they showed dense engraftment in various tissues. The relative ease of recovery and the expression profiles of various markers justify further exploration of IDPSC for clinical therapy.
- Published
- 2006