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283 results on '"GUEDES-PINTO, H."'

7. Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed

Author

Guedes-Pinto H., Bastos E., Cravador A., and Azevedo J.

Subjects
sheep, land-races, polymorphism, genetic-variation, somatotropin, hormone-receptors, genes, dna, pcr, animal-breeding, portugal, acids, bovidae, breeds-animals, caprinae, cell-structure, chromosomes, domestic-animals, europe, hormones, livestock, mammals, nucleic-acids, nucleic-compounds, nucleus, organic-acids, pituitary-hormones, proteins, ruminants, southern-europe, taxa, useful-animals, western-europe, Biotechnology, TP248.13-248.65, Environmental sciences, GE1-350
Abstract

Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) ""Churra da Terra Quente"" was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from genomic DNA and PCR amplification of seven fragments: exon 1 of the alpha-lactalbumin gene; exons 10 and 11 of the alpha s1-casein gene; exon 7 of the beta-casein gene; exon 4 of the kappa-casein gene; exons 4 and 5 of the growth hormone gene and exon 6 of the growth hormone receptor gene. Polymorphisms were detected in five of the seven PCR products. Only kappa-casein and growth hormone receptor were monomorphic. Alpha-lactalbumin and alpha s1-casein exons showed three conformational patterns, beta-casein and growth hormone exon 4 showed two electrophoretic patterns and growth hormone exon 5 showed five conformational patterns. These data provide evidence that ""Churra da Terra Quente"" has a high genetic variability, which opens interesting prospects for future selection programs and also for preservation strategies. Also, our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability.

Published
2001

27. Molecular cloning of TaMATE2 homoeologues potentially related to aluminium tolerance in bread wheat (Triticum aestivum L.).

Author

Garcia‐Oliveira, A. L., Benito, C., Guedes‐Pinto, H., and Martins‐Lopes, P.

Subjects
BREAD, ALUMINUM, MOLECULAR cloning, PLANT chromosomes, PLANT shoots
Abstract

Abstract: Recently, members of the MATE family have been implicated in aluminium (Al) tolerance by facilitating citrate efflux in plants. The aim of the present work was to perform a molecular characterisation of the MATE2 gene in bread wheat. Here, we cloned a member of the MATE gene family in bread wheat and named it TaMATE2, which showed the typical secondary structure of MATE‐type transporters maintaining all the 12 transmembrane domains. Amplification in Chinese Spring nulli‐tetrasomic and ditelosomic lines revealed that TaMATE2 is located on the long arm of homoeologous group 1 chromosomes. The transcript expression of TaMATE2 homoeologues in two diverse bread wheat genotypes, Barbela 7/72/92 (Al‐tolerant) and Anahuac (Al‐sensitive), suggested that TaMATE2 is expressed in both root and shoot tissues of bread wheat, but mainly confined to root rather than shoot tissues. A time‐course analysis of TaMATE2 homoeologue transcript expression revealed the Al responsive expression of TaMATE2 in root apices of the Al‐tolerant genotype, Barbela 7/72/92. Considering the high similarity of TaMATE2 together with similar Al responsive expression pattern as of ScFRDL2 from rye and OsFRDL2 from rice, it is likely that TaMATE2 also encodes a citrate transporter. Furthermore, the TaMATE2‐D homoeologue appears to be near the previously reported locus (wPt0077) on chromosome 1D for Al tolerance. In conclusion, molecular cloning of TaMATE2 homoeologues, particularly TaMATE2‐D, provides a plausible candidate for Al tolerance in bread wheat that can be used for the development of more Al‐tolerant cultivars in this staple crop. [ABSTRACT FROM AUTHOR]

Published
2018
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34. Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)4, (GT)7 and (TA)10 chromosome patterns

Author

Bouilly, Karine, Chaves, R., Leitao, A., Abdellah Benabdelmouna, and Guedes Pinto, H.

Subjects
Chromosome identification, Mollusks, animal structures, Fluorescence in situ hybridization (FISH), Crassostrea gigas, fungi, food and beverages, Simple sequence repeats (SSRs)
Abstract

Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.

Published
2008

36. [Genetic basis of ankylosing spondylitis]

Author

Fp, Santos, Bastos E, Dario Ligeiro, Af, Mourão, Chaves R, Trindade H, Guedes-Pinto H, and Jc, Branco

Subjects
Major Histocompatibility Complex, HLA-B Antigens, Humans, Spondylitis, Ankylosing, HLA-B27 Antigen
Abstract

Ankylosing spondylitis (AS) is a common rheumatic condition, highly heritable. Much of the genetic contribution to the disease lies in the major histocompatibility complex (MHC). The association with the allele group HLA-B*27 has been described worldwide for 30 years. On the other hand, genome wide scans have provided some interesting results showing that other MHC and non-MHC genes could be implicated either in disease susceptibility and phenotypic manifestations. Different hypothesis for disease pathophysiology have been investigated which contribute for a better understanding of the genetic basis of AS. This review aims to summarize the status of the knowledge in this exciting area. New data may, in a near future, change the screening of patients and generate new insights for the emergence of novel therapies.

Published
2007

40. Cytogenetic characterization, frequency and distribution of rob t(1;29) in some Italian and Portuguese cattle breeds

Author

IANNUZZI, L., RANGEL-FIGUEIREDO, M.T., Di Meo, G.P., MOLTENI, L., Perucatti, A., DE GIOVANNI, A.M., Chaves, R., ADEGA, F., SUCCI, G., GUEDES-PINTO, H., Eggen, Andre, ProdInra, Migration, Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

41. Interspecific hybridization in oysters: Restriction enzyme digestion chromosome banding confirms Crassostrea angulata x Crassostrea gigas F1 hybrids

Author

Leitao, A, Chaves, R, Santos, S, Guedes Pinto, H, Boudry, Pierre, Leitao, A, Chaves, R, Santos, S, Guedes Pinto, H, and Boudry, Pierre

Abstract

The taxonomic status of the two commercially important cupped oysters, Crassostrea angulata, the Portuguese oyster (Lamarck, 1819) and Crassostrea gigas, the Japanese oyster (Thunberg, 1793) has long been in question. The recent observation of the hybridization between C. gigas and C. angulata and the production of fertile F1s led us to search for cytogenetic evidence of both parental genomes in the interspecific hybrids. The cytogenetic characterization of the hybrids was performed by the use of restriction endonuclease treatments. This technique has recently shown the potential for individual chromosome identification by banding in oysters. Chromosomes of C. gigas, C. angulata and their hybrids were treated with two different restriction enzymes (ApaI and HaeIII), stained with Giemsa, and examined for banding patterns. These chromosome markers allowed the parental haploid sets to be identified in the hybrids. The analysis of the banded karyotypes of the interspecific hybrids showed that for each chromosome pair, one of the homologues presented a banding pattern consistent with that of C. gigas and the other homologue presented a banding pattern consistent with that of C angulata. These cytogenetic results substantiate the reported interspecific hybridization between C. gigas and C. angulata. In view of these results and taking into account the present expansion of C. gigas aquaculture in southern Europe, the question of the need for preservation of pure C. angulata stocks should be raised as only a few populations remain in the south of Spain and Portugal. Recently, changes in the genetic composition of populations in southern Portugal have indeed been observed, showing that human activities have created contact zones between the two taxa while no natural sympatric zones exist in Europe. (c) 2007 Elsevier B.V. All rights reserved.

Published
2007
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44. Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas

Author

Bouilly, Karine, Leitao, Alexandra, Mccombie, Helen, Chaves, R., Guedes Pinto, H, Boudry, Pierre, Lapegue, Sylvie, Bouilly, Karine, Leitao, Alexandra, Mccombie, Helen, Chaves, R., Guedes Pinto, H, Boudry, Pierre, and Lapegue, Sylvie

Abstract

In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species

Published
2004

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