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Objective: To construct a secretory eukaryotic expression vector of DSG2 fused with the Fc region of the human IgG, to validate its expression in 293T cells, and to purify the secretory protein with biological activity. Methods: The DSG2 extracellular domain fragment gene (DSG2ex), was amplified by PCR, and was inserted into the eukaryotic expression plasmid pCMV3-IgG1 to construct the recombinant eukaryotic expression plasmid-pCMV3-DSG2ex-IgG1. The successfully constructed eukaryotic expression plasmid was transfected into 293T cells to express and secrete DSG2 extracellular domain protein. The targeted protein was purified from the cell culture supernatant by Protein A affinity chromatography and confirmed by Western Blotting and ELISA. Results: The pCMV3-DSG2ex-IgG1 eukaryotic expression plasmid was successfully constructed. The highest protein expression level was obtained with 293T cells after 96 h of transfection. The relative molecular mass of the purified product was between 100 and 130 kDa estimated by SDS-PAGE, which was consistent with the expectation. The yield of the purified protein reached 0.8 mg/mL with a purity over 90%. The purified DSG2 extracellular domain protein with IgG1 tag was recognized by IgG monoclonal antibodies by Western blotting. Moreover, the ELISA results showed that the prepared DSG2 extracellular domain protein had significant binding activity to human type 55 adenovirus Fiber Knob protein (HAdV-55).Conclusion: A simple and efficient method for eukaryotic expression and purification of human soluble DSG2 extracellular domain protein was successfully established, and biologically active DSG2 extracellular domain protein was purified, which laid the foundation for the later study of its protein function and anti-adenovirus drugs. [ABSTRACT FROM AUTHOR]

A new Brønsted acid-catalyzed oxo-cyclization of propargyl alcohols with azlactones to synthesize C 2 -azlactonized 2 H -chromenes has been established that uses 1,1'-binaphthyl-2,2'-diyl hydrogen phosphate (BiNPO 4 H) as the catalyst and gives excellent diastereoselectivities (≥19:1 dr) in most cases. This protocol has a high compatibility with various substituents of substrates, offering a catalytic and useful entry to the fabrication of the synthetically important C 2 -functionalized 2 H -chromene scaffold.

Purpose: To analyze and compare the difference of condylar position between Angle Class I and Class II malocclusion patients using cone-beam CT (CBCT)., Methods: Thirty Class I patients, 30 Class II division 1 patients and 30 Class II division 2 patients were selected in this study. Each patient underwent CBCT. The images in the oblique position perpendicular to the condyloid process were reconstructed by Examvision software. The joint space was measured by Exam Vision software. The data were processed with SPSS17.0 software package., Results: The upper joint space was larger in Class II, the posterior joint space was smaller in Class II patients; and in Class II division 1 patients, both of the upper and anterior joint spaces were larger than in Class II division 2 patients，the differences were significant (P<0.05). The length of condyle was longer in Class I patients than in Class II patients., Conclusions: The condylar position in Class II division 2 patients was lower and further backward. The length of condyle is shortest in Class II division 2 patients.

Purpose: To clarify whether trigeminal somatosensory function will be changed after orthodontic treatment as well as after orthognathic surgery., Methods: A series of 20 patients with a mean age of 20.9±1.74 years were recruited. Thermal quantitative sensory tests were conducted before orthodontic treatment (T0), before orthognathic surgery (T1), 2 months after orthognathic surgery (T2), and 6 months after orthognathic surgery (T3). The cold detection threshold (CDT), cold pain threshold (CPT), warm detection threshold (WDT) and heat pain threshold (HPT) were determined. SPSS 16.0 software package was used for statistical analysis., Results: There were significant differences with lower threshold (higher sensitivity) in V2L (P=0.006) and V2R (P=0.017) for WDT at T1 compared to T0. A significantly higher threshold (lower sensitivity) was detected for CDT in V2L (P=0.028) at T1 compared to T0. A significantly higher threshold (lower sensitivity) was detected for HPT in V2R (P=0.028) and V3R (P=0.017) at T1 compared to T0. There were significant differences with higher threshold (lower sensitivity) V3L (P=0.024) for WDT at T3 compared to T0. A significantly lower threshold (higher sensitivity) was detected for CPT in V2R (P=0.036), V3L (P=0.012) and V3R (P=0.044) at T3 compared to T0. There were significant differences with higher threshold (lower sensitivity) in V2L (P=0.009), V2R (P=0.034), V3L (P=0.032) and V3R (P=0.001) for HPT at T3 compared to T0.Numbness was reported most frequently at T2 (93.33%), but this symptom tended to decrease with time (T3-20%).Fisher's exact test showed that there was no significant difference at T2(P=0.303) and T3(P=0.530) between participates who underwent genioplasty or not., Conclusions: The results indicated that thermal somatosensory function was altered after initial orthodontic treatment and orthognathic surgery. However, partial recovery of thermal sensory function can be expected in the majority of patients.