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Copyright of Canadian Water Resources Journal / Revue Canadienne des Ressources Hydriques is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Herbicide flashback is an undesirable response that results in damage or mortality of non-target plants near chemically treated plants. In severe instances, crop trees can be injured resulting in financial loss or inability to capture management objectives. Land managers need to know the potential limitations of using triclopyr acid (50% solution in water) and what quantity can cause damage to non-target trees (trees not aimed at for control via herbicide). Three naturally regenerated (previously clearcut) bottomland hardwood sites, ranging from 14–20 years of age, received partial overstory deadening using Trycera® herbicide. Residual "leave" trees experienced indirect herbicide uptake resulting in some trees either experiencing mortality or partial mortality with top dieback/epicormic branching. An increased probability of damage occurred as tree diameter decreased and the number of treated stems around the non-target stems increased. American sycamore, green ash, and sweetgum were most susceptible to flashback. This study examines the unanticipated chemical effect of deadening adjacent stems surrounding residual leave trees in poorly drained soils. [ABSTRACT FROM AUTHOR]

The North American gall mite Aceria fraxiniflora was first recorded in Europe in southeast Hungary in 2017. Since then, it has shown a remarkably rapid spread on its host, the also North American green ash (Fraxinus pennsylvanica). By the beginning of 2023 it has been recorded in eight Central-Eastern European countries. In 2022 it was recorded on the other North American ash (Fraxinus Americana) in Zagreb (Croatia) and in Szarvas Arboretum (SE Hungary). Possible reasons and outcomes of this spread are discussed. [ABSTRACT FROM AUTHOR]

Green ash (Fraxinus pennsylvanica) is the most widely distributed ash tree in North America. Once common, it has experienced high mortality from the non‐native invasive emerald ash borer (EAB; Agrilus planipennis). A small percentage of native green ash trees that remain healthy in long‐infested areas, termed "lingering ash," display partial resistance to the insect, indicating that breeding and propagating populations with higher resistance to EAB may be possible. To assist in ash breeding, ecology and evolution studies, we report the first chromosome‐level assembly from the genus Fraxinus for F. pennsylvanica with over 99% of bases anchored to 23 haploid chromosomes, spanning 757 Mb in total, composed of 49.43% repetitive DNA, and containing 35,470 high‐confidence gene models assigned to 22,976 Asterid orthogroups. We also present results of range‐wide genetic variation studies, the identification of candidate genes for important traits including potential EAB‐resistance genes, and an investigation of comparative genome organization among Asterids based on this reference genome platform. Residual duplicated regions within the genome probably resulting from a recent whole genome duplication event in Oleaceae were visualized in relation to wild olive (Olea europaea var. sylvestris). We used our F. pennsylvanica chromosome assembly to construct reference‐guided assemblies of 27 previously sequenced Fraxinus taxa, including F. excelsior. Thus, we present a significant step forward in genomic resources for research and protection of Fraxinus species. [ABSTRACT FROM AUTHOR]

A greenhouse experiment was designed to determine the interactive effect of light, flooding, and competition on the growth and performance of Chinese tallow (Triadica sebifera [L.] Roxb.) and three tree species native to the southeastern United States: water tupelo (Nyssa aquatica L.), sugarberry (Celtis occidentalis L.), and green ash (Fraxinus pennsylvanica Marshall). The experiment used a factorial design that received two treatments: light (low irradiance or high irradiance) and flood (nonflooded and flooded) regimes. In the nonflooded and high irradiance treatment, changes in the growth (ground diameter, number of leaves, and total biomass) indicated that growth metrics of tallow were highest when growing with sugarberry and water tupelo but decreased when tallow was in competition with green ash. In contrast, competition with tallow reduced the height, net photosynthetic rate, stomatal conductance, and transpiration rate of water tupelo. The results showed that tallow had lower growth metrics when in competition with green ash at no apparent decrease in the growth of green ash except for growth rate. Our results suggest that tallow may be less competitive with certain native species and underplanting may be a possible opportunity for improving the success rates of native tree species establishment in areas prone to tallow invasion. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Forestry Society of Croatia / Sumarski List Hrvatskoga Sumarskoga Drustva is the property of Forestry Society of Croatia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Because invasive tree species are being suppressed all over the world, there is a lack of basic information needed for their use in the processing industry. One piece of important information for woodworking applications is the air-dry density, which is 653 kg/m3 in the case of tree of heaven (Ailanthus altissima), 536 kg/m3 for box elder (Acer negundo), and 702 kg/m3 for green ash (Fraxinus pennsylvanica). The order of the 3 species is the same for oven-dry and basic density. In terms of compression and bending, tree of heaven has higher values than green ash. Because the strength of the tree of heaven and the green ash are largely the same as the common ash (Fraxinus excelsior), it can be replaced by these tree species. The properties of box elder wood are significantly different from those of

Because invasive tree species are being suppressed all over the world, there is a lack of basic information needed for their use in the processing industry. One piece of important information for woodworking applications is the air-dry density, which is 653 kg/m3 in the case of tree of heaven (Ailanthus altissima), 536 kg/m3 for box elder (Acer negundo), and 702 kg/m3 for green ash (Fraxinus pennsylvanica). The order of the 3 species is the same for oven-dry and basic density. In terms of compression and bending, tree of heaven has higher values than green ash. Because the strength of the tree of heaven and the green ash are largely the same as the common ash (Fraxinus excelsior), it can be replaced by these tree species. The properties of box elder wood are significantly different from those of sycamore (Acer pseudoplatanus), so this tree species is not suitable for replacement. [ABSTRACT FROM AUTHOR]

Aculus epiphyllus (Nalepa, 1892) is reported from green ash in Europe for the first time. Ash rust mite has never been found on green ash, and we therefore re-describe and illustrate the female and male, and describe the nymph. The species was collected from the underside of the leaflets of the introduced ash species, Fraxinus pennsylvanica Marshall (Oleaceae) in Hungary. [ABSTRACT FROM AUTHOR]

We report a 6-year study of an irruptive invasion of emerald ash borer (EAB) into a 36-year-old comparison of 60 green ash (Fraxinus pennsylvanica Marsh.) and 5 white ash (F. americana L.) populations. As the infestation progressed, annual measures of EAB injury (density of adult emergence holes on the trunk, crown condition, and mortality) were significantly influenced by genetic effects (population and in some instances family within population), site quality (field blocks), and neighborhood (contagion effects over distances of 5–13 m). At the last measurement, 99% of green ash trees and 87% of white ash were dead, and most of the remaining few trees had badly deteriorating crowns. Although final destruction was nearly complete, the rapidity with which trees were injured and killed was moderated by genetic and site effects and influenced by proximity to infested trees. These facts suggest that some genotypes, especially on favorable sites, will disproportionately survive under future equilibrium conditions with lower densities of ash and EAB in the landscape. [ABSTRACT FROM AUTHOR]

Woody plant composition, trees per ha, and basal area, are provided for both units of the W.L. Giles Bur Oak Preserve, in the Black Belt Prairie region of Oktibbeha County, Mississippi. A total of 1,273 individual trees were marked, measured, and identified between the two units of the preserve. The southern unit contained 1,115 trees from 31 species and was dominated both numerically and in basal area by sugarberry (Celtis laevigata) and green ash (Fraxinus pennsylvanica). The northern unit contained 158 trees from 18 species and was dominated by sugarberry, eastern redcedar (Juniperus virginiana), and bur oak (Quercus macrocarpa) respectively in terms of both abundance and basal area. [ABSTRACT FROM AUTHOR]

Highlights • Develop and evaluate stemflow sensing system using open-source methods. • Open-source technology enables high-temporal-resolution environmental monitoring. • New sensing system applied to analyse stemflow generation by one tree during a single storm. Abstract This study develops and evaluates a sensing system capable of measuring stemflow at high temporal resolutions. Leveraging affordable hobbyist grade electronics has allowed for the development of a system which is low-cost, easy to reproduce and adaptable. Eschewing classic stemflow measurement techniques, the system demonstrated herein utilizes a sensing payload which includes a wetness sensor and ultrasonic rangefinder. Combined, these sensors are capable of determining precise stemflow initiation and cessation times as well as capturing high temporal resolution stemflow volume measurements at 10 s intervals. A case study focusing on stemflow data collected by the sensing system from an isolated green ash (Fraxinus pennsylvanica Marshall) during a May 2016 rainfall event in Kamloops, British Columbia is used to evaluate the performance of the sensing system, demonstrating the accuracy of the collected data and the potential research questions that can be addressed by large scale deployment of the sensor system. [ABSTRACT FROM AUTHOR]

Key message: The genetic linkage map for green ash (Fraxinus pennsylvanica) contains 1201 DNA markers in 23 linkage groups spanning 2008.87cM. The green ash map shows stronger synteny with coffee than tomato.Abstract: Green ash (Fraxinus pennsylvanica) is an outcrossing, diploid (2n = 46) hardwood tree species, native to North America. Native ash species in North America are being threatened by the rapid spread of the emerald ash borer (EAB, Agrilus planipennis), an invasive pest from Asia. Green ash, the most widely distributed ash species, is severely affected by EAB infestation, yet few genomic resources for genetic studies and improvement of green ash are available. In this study, a total of 5712 high quality single nucleotide polymorphisms (SNPs) were discovered using a minimum allele frequency of 1% across the entire genome through genotyping-by-sequencing. We also screened hundreds of genomic- and EST-based microsatellite markers (SSRs) from previous de novo assemblies (Staton et al., PLoS ONE 10:e0145031, 2015; Lane et al., BMC Genom 17:702, 2016). A first genetic linkage map of green ash was constructed from 90 individuals in a full-sib family, combining 2719 SNP and 84 SSR segregating markers among the parental maps. The consensus SNP and SSR map contains a total of 1201 markers in 23 linkage groups spanning 2008.87 cM, at an average inter-marker distance of 1.67 cM with a minimum logarithm of odds of 6 and maximum recombination fraction of 0.40. Comparisons of the organization the green ash map with the genomes of asterid species coffee and tomato, and genomes of the rosid species poplar and peach, showed areas of conserved gene order, with overall synteny strongest with coffee. [ABSTRACT FROM AUTHOR]

Emerald ash borer (EAB) (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae), an invasive phloem-feeding buprestid, has killed hundreds of millions of ash (Fraxinus spp.) trees in the United States and two Canadian provinces. We evaluated EAB persistence in post-invasion sites and compared EAB adult captures and larval densities in 24 forested sites across an east-west gradient in southern Michigan representing the Core (post-invasion), Crest (high EAB populations), and Cusp (recently infested areas) of the EAB invasion wave. Condition of green ash (Fraxinus pennsylvanica Marsh) trees were recorded in fixed radius plots and linear transects in each site. Ash mortality was highest in Core sites in the southeast, moderate in Crest sites in central southern Michigan, and low in Cusp sites in the southwest. Traps and trap trees in Crest sites accounted for 75 and 60% of all EAB beetles captured in 2010 and 2011, respectively. Populations of EAB were present in all Core sites and traps in these sites captured 13% of all beetles each year. Beetle captures and larval densities at Cusp sites roughly doubled between 2010 and 2011, reflecting the increasing EAB populations. Sticky bands on girdled trees captured the highest density of EAB beetles per m² of area, while baited double-decker traps had the highest detection rates and captured the most beetles. Larval densities were higher on girdled ash than on similar ungirdled trees and small planted trees. Woodpecker predation and a native larval parasitoid were present in all three invasion regions but had minor effects on ash survival and EAB densities. [ABSTRACT FROM AUTHOR]

The goal of this paper is to apply dendroclimatological methods to the analysis of two commonly planted shelterbelt tree species, Fraxinus pennsylvanica (green ash), and Picea glauca (white spruce), to assess their current relationship with climate and determine how their growth may be affected by climate change in the moisture limited region of southeastern Saskatchewan. Spring precipitation and more importantly spring drought, as represented by the standardized precipitation evapotranspiration index (SPEI), were found to be the most important factors controlling the growth of green ash and white spruce in southeastern Saskatchewan. Furthermore, a breakdown in the radial growth-climate relationship was observed in individuals planted far from their typical native ranges, a potential indication of climate induced stress. Considering these findings, and projections of future climate, it is suggested that conditions beyond the northern limit of the artificial green ash range, and into the boreal forest, may become more suitable for green ash growth, while the southern limit of the artificial white spruce range is expected to recede northward. This information can help guide the management of shelterbelt systems in the Canadian Prairies to ensure they provide maximum practical and ecological benefits for now and into the future. [ABSTRACT FROM AUTHOR]

Decomposition of allochthonous organic matter that enters shaded headwater streams during a short autumn leaf fall period provides much of the energy the streams receive throughout the year. As such, alterations of riparian communities, including those resulting from invasive species, should have a significant impact on these energy inputs and potentially alter microbial communities and the behavior of shredders that process leaf litter. We compared consumption of leaves of an abundant native species (green ash Fraxinus pennsylvanica) and nonnative species (common buckthorn Rhamnus cathartica) by the northern spring amphipod Gammarus pseudolimnaeus, with and without periods of stream conditioning. Amphipods consumed a very small proportion of the unconditioned leaves of common buckthorn and green ash and demonstrated no significant preference for the unconditioned leaves of either species. When leaves were stream-conditioned, there was a significant interaction between the effects of leaf species and days of stream conditioning on both the mass and area of leaf disks consumed by the amphipods. Overall there was a greater consumption of buckthorn leaves compared to ash, with peak consumption occurring during a narrower range of conditioning times for buckthorn. Common buckthorn's higher overall consumption level is expected to provide rapid and short-lived input of energy into the stream, in marked contrast to the slower and more sustained input from native green ash. These results suggest there may be significant changes in the organic matter dynamics of stream ecosystems throughout the Midwest as common buckthorn continues its expansion and green ash declines with the invasion of emerald ash borers ( Agrilus planipennis). [ABSTRACT FROM AUTHOR]

Emerald ash borer (EAB) (Agrilus planipennis), first identified near Detroit, Michigan, U.S., in 2002, has killed millions of ash trees (Fraxinus spp.) in 28 states and two Canadian provinces to date. Trunk injections of insecticide products containing emamectin benzoate (EB) (e.g., TREE-äge®) are often used to protect ash trees in landscapes from EAB, but wounds and potential injury resulting from injections are a concern. Researchers examined 507 injection sites on 61 trees and recorded evidence of secondary wounding (e.g., external bark cracks, internal xylem necrosis and pathogen infection). Researchers assessed 233 injection sites on 22 green ash and 24 white ash trees macro-injected with a low or a medium-high rate of EB in 2008 only, or in both 2008 and 2009. Only 12 of 233 injection sites (5%) were associated with external bark cracks and there was no evidence of pathogen infection. On 39 of the 46 trees (85%), new xylem was growing over injection sites. Researchers assessed 274 injection sites on 15 green ash trees injected annually with EB from 2008 to 2013 or injected in 2008 and again in 2011. Bark cracks were associated with four injection sites on three trees, but no evidence of injury was found on the other 12 trees. All 15 trees had new xylem laid over injection sites. Confocal laser scanning and polarizing digital microscopy were used to assess the integrity of discolored xylem tissue removed from the immediate area surrounding 140 injection sites on 61 trees. Researchers found no evidence of decay associated with discoloration. [ABSTRACT FROM AUTHOR]

A recent invader to North America, emerald ash borer Agrilus planipennis Fairmaire ( Buprestidae), is expanding its western range into new habitats. We examined how site factors affect the emergence and damage caused by this beetle on the western edge of its contiguous population in eastern Wisconsin, U.S.A. We characterized forest structure and quantified signs and symptoms of A. planipennis from 2010 to 2013., We evaluated the potential for establishment of three host-specific parasitic Hymenoptera, released as biological control agents of A. planipennis. During June to September 2011, we conducted releases of 1500 Oobius agrili Zhang and Huang ( Encyrtidae), 1900 Spathius agrili Yang ( Braconidae) and 2700 Tetrastichus planipennisi Yang ( Eulophidae) at the same location., Signs and symptoms of A. planipennis increased in abundance during the present study. Emergence of A. planipennis was greater in larger diameter trees and in subplots with more ash trees. More epicormic shoots were on the upper halves of trees in subplots with a higher percentage of ash. Fraxinus nigra exhibited a higher incidence of ruptured bark than Fraxinus pennsylvanica., In January 2013, we felled four infested ash trees and censused insect emergence from the entire trees. We recovered 441 A. planipennis, 41 T. planipennisi and no other released parasitoids., These results suggest that, of these three parasitoids, T. planipennisi has the highest likelihood of contributing to biological control in Wisconsin, although ash mortality will proceed rapidly and likely supersede the effects of the parasitoids. The best opportunity for introduced parasitoids to benefit Wisconsin may arise in post-outbreak, recovering forests. [ABSTRACT FROM AUTHOR]

We investigated growth, water relations, and water use of bare root (BR) and balled-burlapped (BB) Patmore green ash following transplanting into an irrigated landscape setting in a high desert climate. Treatments were green ash harvested as larger (40 mm caliper) BB and BR trees, and smaller BR stock (25 mm caliper). During establishment, we measured canopy growth for 3 years, and for 2 years plant water relations [predawn water potential and midday stomatal conductance (gS)] and water depletion within the root zone. All treatments expressed varying degrees of isohydric responses to root loss by reducing gS that maintained water potential nearly constant, but least so for the smaller BR trees. gS was greater than that of all larger trees, meaning that for the same cost in water potential as the larger trees, BR-Small benefitted from more open stomates and presumably greater carbon gain. Greater initial conductance apparently translated into more growth. Year 1, BR-Small trees had the least total leaf area, but by Year 3 total leaf area was not different among all treatments. Also during Year 1, the ratio of water use to local reference evapotranspiration [plant factor (PF)] was 0.36 for large BR trees vs. 0.56 for BB trees, similar to the recommended PF of 0.5 for trees in dry climates. These results suggest smaller BR trees are a cheaper alternative for high desert landscapes while reaching nearly equivalent growth to BB trees after 3 years. Achieving high growth of BR trees would need careful scheduling of irrigation amount and frequency based on leaf area, root zone size, and local reference evapotranspiration. [ABSTRACT FROM AUTHOR]

Background: To develop a set of transcriptome sequences to support research on environmental stress responses in green ash (Fraxinus pennsylvanica), we undertook deep RNA sequencing of green ash tissues under various stress treatments. The treatments, including emerald ash borer (EAB) feeding, heat, drought, cold and ozone, were selected to mimic the increasing threats of climate change and invasive pests faced by green ash across its native habitat. Results: We report the generation and assembly of RNA sequences from 55 green ash samples into 107,611 putative unique transcripts (PUTs). 52,899 open reading frames were identified. Functional annotation of the PUTs by comparison to the Uniprot protein database identified matches for 63 % of transcripts and for 98 % of transcripts with ORFs. Further functional annotation identified conserved protein domains and assigned gene ontology terms to the PUTs. Examination of transcript expression across different RNA libraries revealed that expression patterns clustered based on tissues regardless of stress treatment. The transcripts from stress treatments were further examined to identify differential expression. Tens to hundreds of differentially expressed PUTs were identified for each stress treatment. A set of 109 PUTs were found to be consistently up or down regulated across three or more different stress treatments, representing basal stress response candidate genes in green ash. In addition, 1956 simple sequence repeats were identified in the PUTs, of which we identified 465 high quality DNA markers and designed flanking PCR primers. Conclusions: North American native ash trees have suffered extensive mortality due to EAB infestation, creating a need to breed or select for resistant green ash genotypes. Stress from climate change is an additional concern for longevity of native ash populations. The use of genomics could accelerate management efforts. The green ash transcriptome we have developed provides important sequence information, genetic markers and stress-response candidate genes. [ABSTRACT FROM AUTHOR]

This study was carried out in Hungary, in an old unmanaged riparian poplar-willow forest during the breeding seasons of 2014 and 2015. The occurrence of two invasive tree species, the green ash and boxelder, is significant in the study area, which influences negatively the populations of native riparian tree species in Central Europe. We studied Great-spotted Woodpecker nest sites in the presence of these invasive species. Throughout the study period, eight and twelve nesting cavity trees were mapped. Trees were recorded in 20-20 circular plots of 0.05 ha both for each mapped nest trees and random plots as well. Species, diameter at breast height and condition were recorded for each tree. Composition and diversity of nest site and random plots were compared. Distributions and preferences were calculated for nest tree use. Most of the recorded trees were invasive. Nest site plots had more native trees compared to random plots. Nest site showed higher diversity in terms of all three variables. Decayed and dead willow and white poplar hybrid trees were preferred for nesting. Diameter at breast height of nest trees was between 30-90 cm. Studies about cavity excavators in transformed habitats have high importance for nature conservation of riparian forests. [ABSTRACT FROM AUTHOR]

Transcription factors play a key role to enable plants to cope with abiotic stresses. DREB2 regulates the expression of several stress-inducible genes and constitutes major hubs in the water stress signalling webs. We cloned and characterized a novel gene encoding the Fp DREB2A transcription factor from Fraxinus pennsylvanica, and a yeast activity assay confirmed its DRE binding and transcription activation. Overexpression of Fp DREB2A in R. pseudoacacia showed enhanced resistance to drought stress. The transgenic plant survival rate was significantly higher than that of WT in soil drying and re-watering treatments. Transgenic lines showed a dramatic change in root architecture, and horizontal and vertical roots were found in transgenic plants compared to WT. The vertical roots penetrated in the field soil to more than 60 cm deep, while horizontal roots expanded within the top 20-30 cm of the soil. A physiological test demonstrated that chlorophyll contents were more gradually reduced and that soluble sugars and proline levels elevated more sharply but malondialdehyde level stayed the same ( P < 0.05). Plant hormone levels of abscisic acid and IAA were higher than that of WT, while gibberellins and zeatin riboside were found to be lower. The root transcriptomes were sequenced and annotated into 2011 differential expression genes ( DEGs). The DEGs were categorized in 149 pathways and were found to be involved in plant hormone signalling, transcription factors, stimulus responses, phenylalanine, carbohydrate and other metabolic pathways. The modified pathways in plant hormone signalling are thought to be the main cause of greater horizontal and vertical root development, in particular. [ABSTRACT FROM AUTHOR]

In settled areas, electrical line safety is maintained by pruning encroaching trees. Identifying key predictors of branch elongation growth rate following pruning would assist in developing predictive models and optimizing pruning cycles. However, measuring branches in trees near electrical lines is complex and challenging. This paper describes an innovative approach using a handheld laser rangefinder to safely and accurately estimate growth from the ground. In-tree and ground-based laser measurements were highly correlated. This was followed by testing for correlations between branch growth response over a number of years after pruning and many biotic and abiotic factors for Fraxinus pennsylvanica, Acer platanoides, and Acer saccharinum, in the city of Montréal, Canada. In a sample of 59 trees, A. saccharinum had the greatest branch growth, followed by F. pennsylvanica, and finally A. platanoides. Branch growth increased following pruning and subsequently strongly declined, with A. platanoides declining the fastest. Branch inclination angle was positively correlated with growth rate for two species, but not for A. saccharinum. Among the types of pruning used, directional pruning techniques resulted in the least branch regrowth rate. Tree diameter was weakly related to branch growth rates. These results suggest that while growth conditions for street trees may be perceived as homogenous, there is substantial variation in branch growth response. This variation may be related to pruning history, or unmeasured abiotic or biotic variables. Estimating pruning cycle duration is a complex task and further work is needed to develop a predictive model for more accurate estimation of return times. [ABSTRACT FROM AUTHOR]

Moser Park is a property in Allen County, Indiana, managed by New Haven Parks and Recreation. We conducted a plant survey within the forested section of the park, identifying and quantifying abundance of understory, midstory, and overstory plants. We calculated species richness and Shannon's index for all three strata and tested for relationships with environmental variables (i.e., canopy cover, soil compaction, litter depth, and light availability). The majority of understory species are typically associated with disturbed habitats, with very little resemblance to natural, pre-settlement habitat (i.e., low coefficient of conservation values). Understory cover was dominated by Rubus allegheniensis. Midstory stratum was less diverse than the understory and was dominated by an abundance of Fraxinus pennsylvanica, which was also an important species in the overstory. The forest at Moser Park matched the Hackberry-American Elm-Green Ash forest type. There was a lack of both Quercus and many Acer species within Moser Park that are common in the region. Because of the distinct soil types, and disturbance history, a relatively uncommon forest type for the region has developed in Moser Park that differs from the majority of forested land surrounding it. [ABSTRACT FROM AUTHOR]

Invasive insects and pathogens have had major impacts on many forest tree species in North America that often affect forest structure and composition. Despite these effects, variation exists in the likelihood that some native species may persist following outbreaks and establishment of exotic insects and pathogens. Emerald ash borer ( EAB; Agrilus planipennis) has killed millions of trees near its introduction point in southeastern Michigan, and several recent studies in the area have predicted functional elimination of green ash ( Fraxinus pennsylvanica) from the landscape. Intensive management in yet unaffected stands that results from such predictions, however, demands data that examine the potential for persistence of the host species in the presence of the invasive. This study examined the potential for persistence of green ash in the presence of EAB by measuring surviving trees, regeneration, and seed rain characteristics in 17 small, near-pure stands of green ash in five consecutive growing seasons. Live trees experienced 58% mortality due to EAB, significantly less than that reported for ash in mixed stands. Approximately 20% of surviving trees exhibited signs of EAB, confirming that EAB mortality has slowed significantly but is ongoing. Sprouting was the dominant mode of ash regeneration in every year, and 27% of large sprouts produced seeds during a mast year in 2011. Advanced regeneration and new seedling establishment resulted in a sizable level of ash regeneration over the five years of the study even when sapling and seedling mortality was taken into account. Seed production was reduced considerably following EAB-caused mortality, but there was no evidence that seed dispersal limited seedling recruitment. These results suggest that the seed-producing ability of small trees and basal sprouts, as well as continued low-level mortality that will retain reduced host density, may allow green ash to persist in the presence of EAB. Although green ash populations and individual trees are unlikely to ever resemble the stature of those prior to EAB, their presence will continue to be an important component of forests on the landscape. [ABSTRACT FROM AUTHOR]

Riparian forested wetlands improve downstream water quality by trapping suspended sediment from adjacent waterways. Waters that transport sediment and nutrients into adjacent wetlands also create wet and dry hydrologic periods and thus, have the potential to impact site productivity. In this study, we used a dendrogeomorphic technique with green ash ( Fraxinus pennsylvanica Marsh.) to estimate sediment accretion for two time periods (1881–2012 and 1987–2012) along a natural levee (35 m from river) and backswamp (75 m from river) and identified the influence of hydrology and climate on radial growth in green ash and water tupelo ( Nyssa aquatica L.) along the Tensaw River in southwestern Alabama. We detected significantly higher sediment accretion rates for the 1987–2012 time period along the natural levee ( p = 0.00; 1.6 cm yr −1 ) and backswamp ( p = 0.03; 1.2 cm yr −1 ) than for the 1881 to 2012 period (0.4 and 0.5 cm yr −1 ). Using previously measured (2010) soil bulk density for the site, estimated mass of sediment trapped per unit area ranged from 55–135 Mg ha −1 yr −1 for the 1987–2012 period and 17–61 Mg ha −1 yr −1 for the 1881–2012 period. We identified positive, significant correlations between green ash radial growth and the number of days the backswamp was flooded (1.4 m stage), days the Tensaw River was at bankfull (2.1 m), and average daily river stage during the overall growing season (April to August) and for the month of April. Green ash radial growth also illustrated a positive, significant response to April precipitation totals demonstrating the overall role of moisture availability just prior to the onset of xylem formation. Green ash trees along the natural levee and backswamp were more responsive to hydrology and climate than water tupelo trees located further in the backswamp, illustrating the potential resistance of water tupelo to perturbations. This study illustrates the important role forested wetlands play in improving water quality through quantification of sediment accretion rates and the potential impact that introduced disturbances (i.e., forest harvest-related disturbances) can have on ecosystem services. [ABSTRACT FROM AUTHOR]

Emerald ash borer (Agrilus planipennis Fairmaire) is an invasive primary pest of North American ash (Fraxinus spp.) trees. Blue ash (F. quadrangulata) is less susceptible to emerald ash borer infestations in the forest than other species of North American ash. Whereas other studies have examined adult host preferences, we compared the capacity of emerald ash borer larvae reared from emerald ash borer eggs in the field and in the laboratory to survive and grow in blue ash and the more susceptible green ash (F. pennsylvanica). Emerald ash borer larval survivorship was the same on both ash species. Mortality due to wound periderm formation was only observed in living field grown trees, but was low (<4%) in both green and blue ash. No difference in larval mortality in the absence of natural enemies suggests that both green and blue ash can support the development of emerald ash borer. Larvae reared from eggs on blue ash were smaller than on green ash growing in the field and also in bolts that were infested under laboratory conditions. In a laboratory study, parasitism rates of confined Tetrastichus planipennisi were similar on emerald ash borer larvae reared in blue and green ash bolts, as were fitness measures of the parasitoid including brood size, sex ratio, and adult female size. Thus, we postulate that emerald ash borer larvae infesting blue ash could support populations of T. planipennisi and serve as a potential reservoir for this introduced natural enemy after most of the other native ash trees have been killed. [ABSTRACT FROM AUTHOR]

The emerald ash borer (EAB; Agrilus planipennis Fairmaire) is a bark and wood boring beetle native to east Asia that was first discovered in North America in 2002. Since then, entire stands of highly susceptible green ash ( Fraxinus pennsylvanica Marshall) have been killed within a few years of infestation. We have identified a small number of mature green ash trees which have been attacked by EAB, yet survived the peak EAB infestation that resulted in mortality of the rest of the ash cohort. Adult landing and feeding preference bioassays, leaf volatile quantification and EAB egg bioassay experiments were used to characterize potential differences in responses of these select 'lingering' green ash trees relative to known EAB susceptible controls. Three selections were identified as being significantly less preferred for adult feeding, but no specific leaf volatile profile was associated with this reduced preference. Egg bioassays identified two ash selections that had significant differences in larval survival and development; one having a higher number of larvae killed by apparent host tree defenses and the other having lower larval weight. Correlation and validation of the bioassay results in replicated plantings to assess EAB resistance in the field is still necessary. However, the differences between lingering ash selections and susceptible controls measured by these bioassays indicate that more than one mechanism is responsible for the increased resistance to EAB that resulted in these selections surviving longer than their counterparts. Efforts to further increase ash resistance to EAB through use of these selections in a breeding program are underway. [ABSTRACT FROM AUTHOR]

We used tree-ring analysis to examine the radial growth response of a common, moderately flood-tolerant species ( Fraxinus pennsylvanica Marsh.) to hydrologic and climatic variability for >40 years before and after hydrologic modifications affecting two forest stands in the Mississippi River Alluvial Valley (USA): a stand without levees below dams and a stand within a ring levee. At the stand without levees below dams, spring flood stages decreased and overall growth increased after dam construction, which we attribute to a reduction in flood stress. At the stand within a ring levee, growth responded to the elimination of overbank flooding by shifting from being positively correlated with river stage to not being correlated with river stage. In general, growth in swales was positively correlated with river stage and Palmer Drought Severity Index (an index of soil moisture) for longer periods than flats. Growth decreased after levee construction, but swales were less impacted than flats likely because of differences in elevation and soils provided higher soil moisture. Results of this study indicate that broadscale hydrologic processes differ in their effects on the flood regime, and the effects on growth of moderately flood-tolerant species such as F. pennsylvanica can be mediated by local-scale factors such as topographic position, which affects soil moisture. [ABSTRACT FROM AUTHOR]

Protection of green ash trees (Fraxinus pennsylvanica Marshall) from the emerald ash borer (EAB), Agrilus planipennis Fairmaire, by soil applications of neonicotinoids (imidacloprid, clothianidin, and dinotefuran) was tested at five locations between 2005 and 2013. Application rate and spring versus fall application dates were evaluated in tests with neighborhood street trees and in one plantation of 65 ash trees. Insecticide treatments of ash trees at all five sites were initiated as the leading edge of the EAB invasion began to kill the first ash trees at each location. Trees were treated and evaluated at each site for 4 to 7 yr. Spring applications of imidacloprid were more efficacious than fall applications. Application rates of 0.8 g a.i./cm dbh or greater per year gave a higher level of protection and were more consistent than rates of 0.56 g a.i./cm dbh per year or less. The number of years between the first observation of canopy loss due to EAB and death of most of the control trees varied from three to seven years among test sites, depending on how many non-treated ash trees were nearby. [ABSTRACT FROM AUTHOR]

We compared the incidence of infestation by emerald ash borer (EAB) and lilac borer on white fringetree to that of its Asian congener, Chinese fringetree, Chionanthus retusus, and a North American relative, devilwood, Osmanthus americanus. We also conducted laboratory bioassays to determine the suitability of these hosts for EAB larvae. At Spring Grove Cemetery and Arboretum in Cincinnati, Ohio, 9 of 28 white fringetrees examined were infested by EAB. Most of the white fringetrees had lilac borer infestation, and most of the trees infested by EAB also had lilac borer infestation. None of the 11 Chinese fringetrees examined were infested by either EAB or lilac borer. Each of the five devilwood individuals examined was infested by lilac borer, but not EAB. At The Morton Arboretum in Lisle, Illinois, 7 of 16 white fringetrees examined were infested by EAB, while none of the seven Chinese fringetrees examined were infested by either insect. A 40-d bioassay confirmed that white fringetree was an acceptable host, producing fourth-instar larvae that were smaller than those produced on a highly susceptible cultivar of green ash, Fraxinus pennsylvanica. No larvae survived on Chinese fringetree, and neonates were largely incapable of feeding on it. Two larvae survived on devilwood, reaching the second instar and excavating extensive galleries. Future work should be aimed at biotic and abiotic factors influencing the susceptibility of white fringetree, as well as further examination of close relatives for their vulnerability to EAB. [ABSTRACT FROM AUTHOR]

The Government of Canada's farm assistance programs have affected >80 % of Canada's agricultural land base. One important program in the Prairie Provinces was the prairie shelterbelt program (PSP). A significant aspect of the PSP was shelterbelt tree planting to protect farmyard infrastructure and reduce soil erosion. The main goal of this paper was to map historical shelterbelt establishment, total expected shelterbelt length, and total expected number of six common planted shelterbelt species: caragana ( Caragana arborescens), green ash ( Fraxinus pennsylvanica), Manitoba maple ( Acer negundo), Scots pine ( Pinus sylvestris), white spruce ( Picea glauca Monch), and hybrid poplar ( Populus spp.). A clustering approach was designed to group all agricultural ecodistricts (106 total) into clusters (31 total) based on their similarity in 42 variables within five soil zones of Saskatchewan. Correlations between trees ordered through the PSP and observed shelterbelt length (across 2.1 Mha cumulative study area) were used for shelterbelt probability mapping. Mapping accuracy of planted shelterbelts was 48-86 %. Total shelterbelt length (of any species) ranged from 322 to 45,231 km for (in descending order) dark brown > brown > black > dark gray > gray soil zones. Novel decadal time-lapse maps and species-specific shelterbelt maps were produced to capture the progression of shelterbelt establishment for the first time at a province-wide scale which gave a new perspective, in map format, of the expansive impact of the living legacy of the PSP. Shelterbelt data gaps and high priority clusters of agricultural land in Saskatchewan were identified for future shelterbelt research. [ABSTRACT FROM AUTHOR]

Quantifying carbon in agroforestry trees requires biomass equations that capture the growth differences (e.g., tree specific gravity and architecture) created in the more open canopies of agroforestry plantings compared with those generally encountered in forests. Whereas forest-derived equations are available, equations for open-grown trees are not. Data from destructively sampled open-grown trees in the Northern Great Plains were used to examine the uncertainties in the use of forest-derived equations for open-grown trees. Three species, representative of major morphological types of agroforestry trees, were studied: green ash, Austrian pine, and eastern redcedar. Forest-derived equations provided good estimates of trunk biomass at lower diameter ranges but, as diameter increased, resulted in overestimation up to 40% for individual trees. Across the full diameter ranges, individual tree branch biomass was underestimated by 29-82%, depending on species and equation source (regional or nonregional). Although open-grown trunk and branch biomass curves diverged down and up, respectively, from their forest-derived counterparts, those for the whole tree tended to converge, albeit significantly above the forest-derived curves. Whole-tree biomass for individual trees was underestimated by at least 18%. To correct the biases, we studied the adjustment factor of forest- to open-grown tree biomass. It shows a power function with diameter. On a whole-tree basis, it was evaluated as a constant (1.2) independent of species and diameters. Application of this constant factor adjusted the biomass underestimation of three-species-mixed plantation by forest-derived equations from 21 to 4.6%, providing a cost-efficient approach to use forest-derived equations for open-grown trees in agriculture land. [ABSTRACT FROM AUTHOR]

A multivariate statistical model was developed to classify seral stages and to monitor succession within the green ash (Fraxinus pennsylvanica Marshall)chokecherry (Prunus virginiana L.)--western snowberry (Symphoricarpos occidentalis Hook.) ecological type on the northern Great Plains. Two key variables, green ash basal area (ft²/acre) and Prunus species (% canopy cover) (Prunus species = chokecherry + American plum (P. americana Marshall)), provide all the information required for the model to classify seral stages and to be used to monitor trends within the ecological type. Te model quantitatively identified four seral stages (early to late succession), all significantly different (P < 0.001), with an overall accuracy of seral stage assignment of 95%. It also showed that succession is not linear, but goes through multiple states among the four seral stages. These four defined seral stages provide resource managers with options to quantitatively evaluate and manage resources to meet objectives and monitoring plans. Te model provides coefficients for application and input to qualitative state and transition models and is simple to use, reliable, repeatable, accurate and cost effective. [ABSTRACT FROM AUTHOR]

The USA is experiencing a prolific invasion of the wood-boring emerald ash borer, Agrilus planipennis. Native to Asia, this beetle completes its life cycle on ash trees and results in nearly complete mortality of all infested trees. In the present study, we examined the levels of genetic diversity and differentiation among eight populations of Fraxinus pennsylvanica (green ash) using five polymorphic microsatellite loci. Genetic information was used to design guidelines for the establishment of a seed collection sampling strategy to conserve the genetic diversity of ash trees. We found high levels of genetic diversity, as indicated by the allelic richness, both across the populations (16.4 ± 5.18 alleles per locus) and within them (8.03 ± 1.21 alleles per locus). The expected and observed heterozygosity was also high (0.805 ± 0.38 and 0.908 ± 0.04, respectively), and there was moderate genetic differentiation among the populations (FST= 0.083) with members of these eight populations grouped into three distinct clusters. We examined the relationship between the number of individuals sampled and the number of alleles captured in a random sample taken from a population of 10,000 individuals. Only sample sizes of 100 individuals captured most of the alleles (average = 78.74 alleles), but only seven of 50 samples effectively captured all the 82 alleles. Smaller samples did not capture all alleles. A probabilistic model was used to determine an optimal sampling strategy, and it was concluded that a collection of 200 seeds from each of five mother trees would have the highest likelihood of capturing all alleles in a population. [ABSTRACT FROM AUTHOR]

Emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), is a devastating buprestid beetle introduced to North America from Asia. Asian ash trees exhibit resistance to EAB, likely due to a shared co-evolutionary history. Resistance to one pest, however, does not necessarily confer resistance to others. Should Asian ash be highly susceptible to North American herbivores, the utility of such species for hybridization, breeding programs, and establishment in managed landscapes could be compromised. Common urban problems, such as drought stress, can increase borer attack and survival and may further complicate the search for resistant plant material. The objectives of this study were to examine the relative susceptibility of green (Fraxinus pennsylvanica) and Manchurian (F. mandshurica) ash to EAB and indigenous borers and whether that susceptibility changed with drought stress. In a common garden experiment, EAB occurred more frequently and reached higher abundances in green than Manchurian ash. The frequency and abundance of bark beetles (Curculionidae), North American native clearwing borers (Sesiidae), and longhorn beetles (Cerambycidae) were similar in the two ash species. Generation time of EAB was uniformly one year and did not depend on ash species or water stress. Although borers increased as expected in stressed trees, the relative susceptibility of green and Manchurian ash to borers did not change. The findings suggest Manchurian ash may be resistant to several classes of borers, regardless of insect geographic origin, although these conclusions should be viewed with some caution until the results can be verified in larger trees. [ABSTRACT FROM AUTHOR]

Assessing emerald ash borer ( Agrilus planipennis Fairmaire) impacts in North American forests is essential for projecting future species composition of stands invaded by this phloem-feeding pest. We surveyed all species of overstory trees and regeneration in 2010 and 2011 in 24 forested sites with a major component of green ash ( Fraxinus pennsylvanica), representing the Core, Crest, and Cusp of the A. planipennis invasion wave across southern Michigan. By 2011, an average of 78.6% ± 0.10%, 44.8% ± 0.11%, and 19.8% ± 0.07% of overstory ash trees representing 87%, 57%, and 14% of the total ash basal area had been killed in Core, Crest, and Cusp sites, respectively. Green ash seedlings, saplings, and recruits were abundant in all sites, but newly germinated ash seedlings were absent in Core sites and scarce in Crest sites. Canopy gaps resulting from current ash decline and mortality increased available photosynthetically active radiation (PAR) and sapling growth in Crest sites, but PAR was low in Core and Cusp sites. Lateral ingrowth of non-ash overstory trees has largely filled canopy gaps in Core sites, and there was little evidence of green ash recruitment into the overstory. Green ash appears unlikely to persist as a dominant species in forests invaded by A. planipennis. [ABSTRACT FROM AUTHOR]

Spathius galinae Belokobylskij & Strazanac is a recently described parasitoid of the emerald ash borer, Agrilus planipennis Fairmaire, in the Russian Far East, and is currently being considered for biocontrol introduction in the United States. Using A. planipennis larvae reared with freshly cut ash (Fraxinus spp.) sticks, we investigated the biology, life cycle, and rearing of S. galinae in the laboratory under normal rearing conditions (25 ± 1°C, 65 ± 10% relative humidity, and a photoperiod of 16:8 [L:D] h). Our study showed that S. galinae took approximately 1 mo (29 d) to complete a single generation (from egg to adult) under the laboratory rearing conditions. After eclosion from eggs, larvae of S. galinae molted four times to reach the fifth instar, which then spun cocoons for pupation and development to adults. Adult female wasps had a median survival time of 7 wk with fecundity peaking 3 wk after emergence when reared in groups (of five females and five males) and 2 wk in single pairs. Throughout the life span, a single female S. galinae produced a mean (±SE) of 31 (±3.0) progeny when reared in groups, and a mean (±SE) of 47 (±5.3) progeny when reared in single pairs. Results from our study also showed that S. galinae could be effectively reared with A. planipennis larvae reared in both green (Fraxinus pennsylvanica Marshall) and tropical [Fraxinus uhdei (Wenzig) Lingelsh] ash sticks. However, the abortion (unemergence) rate of S. galinae progeny was much higher (20%) when reared with host larvae in green ash sticks than that (2.1%) in tropical ash sticks. [ABSTRACT FROM AUTHOR]

Forty-two Populus spp. clones, Eucalyptus benthamii, and seven tree species native to North Carolina were evaluated for survival and height growth through the establishment phase at two municipal wastewater application sites. Groundwater was monitored at each site to determine if establishment of the species trials resulted in exceedances of nutrient mitigation requirements. At the Gibson Wastewater Treatment Facility, 26 Populus clones had 100 % survival, with mean height growths ranging between 152 to 260 cm, and basal diameters ranging between 11.4 and 28.8 mm. Green ash, planted in 2011 and 2012, had high survivorship (>95 %) with first year mean height growth of 30 ± 28 cm (2012) and second year mean height growth of 101 ± 52 cm (2011). Basal diameter for green ash was 33.3 ± 12.6 mm. E. benthamii had moderate survivorship (>77 %) and first year mean height growth of 47 ± 27 cm. At the Jacksonville Wastewater Treatment Facility, green ash and bald cypress had high survivorship (>96 %), first year mean height growths of 14 ± 25 cm and 27 ± 16 cm, and basal diameters of 13.1 ± 3.9 mm and 11.6 ± 4.8 mm, respectively. Survivorship for 12 Populus clones ranged from 50 and 94 % with mean first year height growths between 58 to 121 cm, and basal diameters between 6.8 and 12.5 mm. E. benthamii had low survivorship (43 %) with mean first year height growths of 17 ± 17 cm and basal diameters of 12.0 ± 7.7 mm. Groundwater concentrations of NO + NO and N-NH remained below regulatory requirements at both sites with one exceedance in February 2012 in Jacksonville, NC. The results show that some Populus clones are excellent candidates for woody biomass production on municipal wastewater application fields. Native green ash and bald cypress are also good candidates, but these trees may require longer rotations than Populus to achieve similar biomass yields. [ABSTRACT FROM AUTHOR]

The present study explores the use of micromorphological leaf epidermal characters of Ficus benjamina (evergreen) and Fraxinus pennsylvanica (deciduous) as indicators of pollution in the Metropolitan Area of Buenos Aires. Mature leaves of both species were sampled along an urban-periurban gradient, with a rural area as control for epidermal characters. Parameters examined from abaxial and upper epidermis were: (1) length and width of stomata, (2) number of stomata per area unit, (3) length and width of epidermal cells, (4) number of epidermal cells per area unit. Data were analysed with basic statistical and multivariate techniques. Principal Component Analysis (PCA) clearly grouped foliar samples according to three zones: urban, periurban and rural areas with different pollution values. No correlations were observed between epidermal character variations and climate variables, air pollutants or C, N, P and metals concentration in soil. Only one positive correlation (p = 0.006) was found between stomata length in leaves from Ficus and iron concentration in soil. [ABSTRACT FROM AUTHOR]

Management strategies to control invasive species need information about dispersal distances to predict establishment potential. Fraxinus pennsylvanica is a North American anemochorous tree species that is invasive in many Central European floodplain forests. To predict seed-dispersal potential, the stochastic model WaldStat was used, which enables different options for directionality (isotropic and anisotropic) to be simulated. In this article, we (1) show empirical results of fructification and seed dispersal for this tree species. The model predicts approximately 250,000 seeds for one F. pennsylvanica tree. These results were used to (2) calculate species-specific dispersal distances and effects of wind direction. To consider the influence of wind on dispersal potential of the tree species, long-distance dispersal (LDD [95th percentile dispersal distance]) was calculated. Mean dispersal distances varied between 47 and 66 m. LDD values modelled along the main wind direction ranged from 60 to 150 m. Seed production, dispersal distance, and direction data were (3) incorporated into theoretical management scenarios for forest ecosystems. Finally (4), we discuss management options and the practical relevance of model scenarios in relation to the accuracy of spatial dispersal predictions. Further analyses should be focused on possible, well-adapted management concepts at stand level that could restrict the potential spread of invasive species. [ABSTRACT FROM AUTHOR]

Highlights: [•] Floating ability facilitates water dispersal. [•] Hydrochorous seed dispersal is more effective than wind dispersal. [•] Storage in water induced germination rate. [ABSTRACT FROM AUTHOR]

Shelterbelts have played an important role in prairie agriculture since the late 1800s; however, little is known about how these shelterbelts may be affected by climate change. The objective of this study was to determine if shelterbelt species, which are heavily influenced by human activity, express a common radial-growth signal within and between trees. The study focused on the annual tree-ring growth of the nine most common shelterbelt species of the Canadian Prairies: Salix acutifolia (Acute willow), Caragana arborescens (caragana, or Siberian pea shrub), Picea pungens (Colorado spruce), Fraxinus pennsylvanica (green ash), Populus sp. (hybrid poplar), Acer negundo (Manitoba maple), Pinus sylvestris (Scots pine), Ulmus pumila (Siberian elm) and Picea glauca (white spruce). Tree core samples were collected near Saskatoon, Saskatchewan using traditional dendrochronological methods. The standardized growth of each species was compared with historical homogenized climate data in order to determine the key monthly climate variables impacting each species. Prior to this analysis, little was known about the suitability of six of these nine species for dendrochronological purposes. It was found that all species crossdate at a significant level, and that the three most significantly correlated climate factors are able to account for up to 37 % of the annual variation in tree-ring growth. The findings of this study suggest that all nine species are suitable, to varying degrees, for future dendrochronological research in the Canadian Prairies as well as having implications for shelterbelt systems elsewhere in the world. The top four species based on four ranking criteria (interseries correlation, mean sensitivity, climate explanatory power, and commonality) were white spruce, acute willow, caragana, and Manitoba maple, and initial results suggest that all species have the potential to be investigated in greater depth. [ABSTRACT FROM AUTHOR]

Green capping is one of the popular methods to re-vegetate abandoned ash ponds of coal based thermal power plants thereby lowering the risk of contamination to the surrounding environment. It has innumerable advantages such as prevention of dust emission, checking soil erosion, stabilizing the surface areas of ash, preventing potential ground water contamination, and finally, adding native vegetation cover, which is very vital in the long term. During the early nineties and later, various reclamation projects were carried out on fly ash dumps, but until date, there have not been any initiatives to assess the alterations in physicochemical and biological properties of fly ash resulting from implementation of these reclamation projects. In the present study, three abandoned ash ponds, located in India, that were reclaimed during 1998–2003 are investigated. Marked alterations in nutritional status, microbial population, and microbial activities have been observed in reclaimed ash ponds. [ABSTRACT FROM AUTHOR]