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Cornus iridoid glycosides (CIGs), including loganin and morroniside, are the main active components of Cornus officinalis. As one of the key enzymes in the biosynthesis of CIGs, geranyl pyrophosphate synthase (GPPS) catalyzes the formation of geranyl pyrophosphate, which is the direct precursor of CIGs. In this study, the C. officinalis geranyl pyrophosphate synthase (CoGPPS) sequence was cloned from C. officinalis and analyzed. The cDNA sequence of the CoGPPS gene was 915 bp (GenBank No. OR725699). Phylogenetic analysis showed that CoGPPS was closely related to the GPPS sequence of Actinidia chinensis and Camellia sinensis, but relatively distantly related to Paeonia lactiflora and Tripterygium wilfordii. Results from the quantitative real-time PCR showed the spatiotemporal expression pattern of CoGPPS; that is, CoGPPS was specifically expressed in the fruits. Subcellular localization assay proved that CoGPPS was specifically found in chloroplasts. Loganin and morroniside contents in the tissues were detected by high-performance liquid chromatography, and both compounds were found to be at higher levels in the fruits than in leaves. Thus, this study laid the foundation for further studies on the synthetic pathway of CIGs. [ABSTRACT FROM AUTHOR]

CROPOS, as the Croatian GNSS network, was modernized and upgraded to support the Galileo system in 2019. Two of CROPOS's services—VPPS (Network RTK service) and GPPS (post-processing service)—were assessed for the contribution of the Galileo system to their performance. A station used for field testing was previously examined and surveyed to determine the local horizon and to carry out a detailed mission planning. The whole day of observation was divided into several sessions, each with a different visibility of Galileo satellites. A special observation sequence was designed: VPPS (GPS-GLO-GAL), VPPS (GAL-only), and GPPS (GPS-GLO-GAL-BDS). All observations were taken on the same station with the same GNSS receiver, Trimble R12. Each static observation session was post-processed in Trimble Business Center (TBC) in two different ways: considering all available systems (GGGB) and considering GAL-only observations. A daily static solution based on all systems (GGGB) was considered as the reference for the accuracy assessment of all obtained solutions. The results obtained with VPPS (GPS-GLO-GAL) and VPPS (GAL-only) were analyzed and assessed; the results obtained with GAL-only have shown a slightly higher scatter. It was concluded that the inclusion of the Galileo system in CROPOS has contributed to the availability and reliability of solutions but not to their accuracy. By complying with the observation rules and taking redundant measurements, the accuracy of GAL-only results can be improved. [ABSTRACT FROM AUTHOR]

Antimicrobial Resistance (AMR) is a growing global health challenge that threatens to undo gains in human and animal health. Prevention and control of AMR requires functional antimicrobial stewardship (AMS) program, which is complex and often difficult to implement in low- and middle-income countries. We aimed to describe the processes of establishing and implementing an AMS program at Connaught Hospital in Sierra Leone. The project involved the setting up of an AMS program, capacity building and performing a global point prevalence survey (GPPS) at Sierra Leone's national referral hospital. Connaught Hospital established a multidisciplinary AMS subcommittee in 2021 to provide AMS services such as awareness campaigns, education and training and review of guidelines. We performed a GPPS on 175 patients, of whom more than half (98, 56.0%) were prescribed an antibiotic: 63 (69.2%) in the surgical wards and 53 (51.2%) in the medical wards. Ceftriaxone (60, 34.3%) and metronidazole (53, 30.3%) were the most common antibiotics prescribed to patients. In conclusion, it is feasible to establish and implement an AMS program in low-income countries, where most hospitalized patients were prescribed an antibiotic. [ABSTRACT FROM AUTHOR]

Abstract Background There is evidence to suggest that the proportion of missing values is slightly higher in the older population resulting in lower completion rates of the EQ-5D. However, existing studies rarely provide a within-sample comparison of feasibility properties across age groups to quantify this difference. Hence, this study examines feasibility properties of the EQ-5D-3L and 5L in the general population and explores the impact of age on the completion of EQ-5D instruments. Methods We pool five waves from the English GP Patient Survey, where respondents self-report their health in either EQ-5D-3L or 5L. Descriptive analysis was undertaken to analyse the distribution and proportion of missing values and completion rates stratified by age and EQ-5D version; logistic regression models were specified to quantify the impact of age, gender and potential long-term conditions on the completion of each of the EQ-5D instruments. Results The total sample comprises ~ 4.36 million observations, of which 2.88 million respondents report their health in 5L and 1.47 million in 3L, respectively. Respondents over 64 years have slightly more missing values in each dimension than younger respondents. The highest share was observed for the oldest age group in the dimension anxiety/depression (3L 9.1% vs. 5L 7.6%), but was otherwise below 5%. Consequently, completion rates (observed and predicted) decreased with older age and at a higher rate after the age of 64; this was more pronounced for the 3L. Conclusion Evidence from our study suggests that both the EQ-5D-3L and 5L have good feasibility properties. In comparison to younger populations there appears to be a higher proportion of respondents with incomplete responses beyond the age of 64 years. Overall, the 5L version compares more favourably in terms of missing values, completion rates as well as with regard to the expected probability of an incomplete descriptive system.

The paucity of in-situ records, particularly in the glaciated mountainous region, is an obstacle in cryosphere ecology and environmental studies. Generally, available gauge station data is fragmented and covers valleys; thus, the use of gridded precipitation products (GPPs) is crucial in such complex terrains. However, these GPPs suffer from systematic biases and uncertainties owing to parameterization deficiencies. Therefore, the main goal of this research is to systematically evaluate the long-term performance and differences of the newly launched MSWEP in comparison to APHRO, CHIRPS, ERA-5, and PGMFD over the transboundary region of Indo-Pak (1981–2009) at spatial (whole to sub-basins) and temporal (daily to seasonal) scales. Findings reveal (1) overall, five GPPs produced well annual spatial precipitation variability with high magnitudes in the northwestern and low in the northeastern region. (2) The estimations from GPPs also divulged better correlation with in-situ observations (MSWEP = 0.86, APHRO = 0.76, ERA-5 = 0.81, CHIRPS = 0.57 and PGMFD = 0.68) at daily span. Better performance was observed during the monsoon compared to winter and pre-monsoon seasons. (3) Lately, estimates from MSWEP are more reliable for all the seasons, especially in the winter season, with the highest CC (0.90) and lowest relative bias (3.03%). (4) All GPPs (excluding ERA-5) overestimated light precipitation (0–1 mm/day) and underestimated moderate to heavy precipitation, in contrast to the ERA-5 that tended to underestimate the light but overestimate moderate (1–20 mm/day) and heavy precipitation (>20 mm/day) events. The CHIRPS was less accurate in detecting most of the precipitation events. The MSWEP product captured all precipitation intensities more accurately than other GPPs. The current research indicates considerable implications for product improvement and data users for choosing better alternative precipitation data at a local scale. [ABSTRACT FROM AUTHOR]

Background: There is evidence to suggest that the proportion of missing values is slightly higher in the older population resulting in lower completion rates of the EQ-5D. However, existing studies rarely provide a within-sample comparison of feasibility properties across age groups to quantify this difference. Hence, this study examines feasibility properties of the EQ-5D-3L and 5L in the general population and explores the impact of age on the completion of EQ-5D instruments. Methods: We pool five waves from the English GP Patient Survey, where respondents self-report their health in either EQ-5D-3L or 5L. Descriptive analysis was undertaken to analyse the distribution and proportion of missing values and completion rates stratified by age and EQ-5D version; logistic regression models were specified to quantify the impact of age, gender and potential long-term conditions on the completion of each of the EQ-5D instruments. Results: The total sample comprises ~ 4.36 million observations, of which 2.88 million respondents report their health in 5L and 1.47 million in 3L, respectively. Respondents over 64 years have slightly more missing values in each dimension than younger respondents. The highest share was observed for the oldest age group in the dimension anxiety/depression (3L 9.1% vs. 5L 7.6%), but was otherwise below 5%. Consequently, completion rates (observed and predicted) decreased with older age and at a higher rate after the age of 64; this was more pronounced for the 3L. Conclusion: Evidence from our study suggests that both the EQ-5D-3L and 5L have good feasibility properties. In comparison to younger populations there appears to be a higher proportion of respondents with incomplete responses beyond the age of 64 years. Overall, the 5L version compares more favourably in terms of missing values, completion rates as well as with regard to the expected probability of an incomplete descriptive system. [ABSTRACT FROM AUTHOR]

Antimicrobial Resistance (AMR) is a growing global health challenge that threatens to undo gains in human and animal health. Prevention and control of AMR requires functional antimicrobial stewardship (AMS) program, which is complex and often difficult to implement in low- and middle-income countries. We aimed to describe the processes of establishing and implementing an AMS program at Connaught Hospital in Sierra Leone. The project involved the setting up of an AMS program, capacity building and performing a global point prevalence survey (GPPS) at Sierra Leone’s national referral hospital. Connaught Hospital established a multidisciplinary AMS subcommittee in 2021 to provide AMS services such as awareness campaigns, education and training and review of guidelines. We performed a GPPS on 175 patients, of whom more than half (98, 56.0%) were prescribed an antibiotic: 63 (69.2%) in the surgical wards and 53 (51.2%) in the medical wards. Ceftriaxone (60, 34.3%) and metronidazole (53, 30.3%) were the most common antibiotics prescribed to patients. In conclusion, it is feasible to establish and implement an AMS program in low-income countries, where most hospitalized patients were prescribed an antibiotic.

The essential oil of Pelargonium graveolens (rose-scented geranium), an important aromatic plant, comprising mainly mono- and sesqui-terpenes, has applications in food and cosmetic industries. This study reports the characterization of isoprenyl disphosphate synthases (IDSs) involved in P. graveolens terpene biosynthesis. The six identified PgIDSs belonged to different classes of IDSs, comprising homomeric geranyl diphosphate synthases (GPPSs; PgGPPS1 and PgGPPS2), the large subunit of heteromeric GPPS or geranylgeranyl diphosphate synthases (GGPPSs; PgGGPPS), the small subunit of heteromeric GPPS (PgGPPS.SSUI and PgGPPS.SSUII), and farnesyl diphosphate synthases (FPPS; PgFPPS).All IDSs exhibited maximal expression in glandular trichomes (GTs), the site of aroma formation, and their expression except PgGPPS.SSUII was induced upon treatment with MeJA. Functional characterization of recombinant proteins revealed that PgGPPS1, PgGGPPS and PgFPPS were active enzymes producing GPP, GGPP/GPP, and FPP respectively, whereas both PgGPPS.SSUs and PgGPPS2 were inactive. Co-expression of PgGGPPS (that exhibited bifunctional G(G)PPS activity) with PgGPPS.SSUs in bacterial expression system showed lack of interaction between the two proteins, however, PgGGPPS interacted with a phylogenetically distant Antirrhinum majus GPPS.SSU. Further, transient expression of AmGPPS.SSU in P. graveolens leaf led to a significant increase in monoterpene levels. These findings provide insight into the types of IDSs and their role in providing precursors for different terpenoid components of P. graveolens essential oil. • Six IDSs that are highly expressed in glandular trichomes of P. graveolens were functionally characterized. • Results revealed geranium has a homomeric GPPS, a bifunctional G(G)PPS, and a FPPS involved in terpenoid precursor formation. • Enzyme kinetics indicated that both homomeric and bifunctional G(G)PPS provide precursors for monoterpene biosynthesis. [ABSTRACT FROM AUTHOR]

Geranyl diphosphate synthase (GPPS) is a plastid localized enzyme that catalyzes the biosynthesis of Geranyl diphosphate (GPP), which is a universal precursor of monoterpenes. Wintersweet (Chimonanthus praecox L.), a famous deciduous flowering shrub with a strong floral scent character, could have GPPS-like homologs that are involved in monoterpenes biosynthesis, but it remains unclear. In the present study, five full-length GPPS and geranylgeranyl diphosphate synthases (GGPPS) genes were identified in the wintersweet transcriptome database. The isolated cDNAs showed high protein sequence similarity with the other plants GPPS and GGPPS. The phylogenetic analysis further classified these cDNAs into four distinct clades, representing heterodimeric GPPS small subunits (SSU1 and SSU2), homodimeric GPPS, and GGPPS. Analysis of temporal expression revealed that all genes have the highest transcript level at the full-open flower stage. From tissue-specific expression analysis, CpGPPS.SSU1 and CpGGPPS1 were predominantly expressed in petal and flower, whereas CpGPPS.SSU2, GPPS, and GGPPS2 showed a constitutive expression. Additionally, the subcellular localization assay identified the chloroplast localization of SSUs and GGPPSs proteins, and the yeast two-hybrid assay showed that both CpGPPS.SSU1 and CpGPPS.SSU2 can interact with the GGPPS proteins. Taken together, these preliminary results suggest that the heterodimeric GPPS can regulate floral scent biosynthesis in wintersweet flower.

The brittle crack initiation from a circular hole in an infinite slab under uniaxial remote tensile load is investigated. The analysis consists of two parts. The former is focused on the difference between symmetric and asymmetric crack propagation. Different criteria in the framework of the Theory of Critical Distances are implemented, and the potentiality of coupled Finite Fracture Mechanics (FFM) approaches is highlighted from a theoretical point of view. The latter presents the experimental results obtained by carrying out ad-hoc tensile tests on polymethyl-methacrylate (PMMA) and general-purpose polystyrene (GPPS) notched samples and the related FFM investigation. It is shown that FFM predictions are in very good agreement with the experimental results for both tested materials. [ABSTRACT FROM AUTHOR]

Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT-specific R2R3-MYB gene, MsMYB, from comparative RNA-Seq data of spearmint and functionally characterized it. Analysis of MsMYB-RNAi transgenic lines showed increased levels of monoterpenes, and MsMYB-overexpressing lines exhibited decreased levels of monoterpenes. These results suggest that MsMYB is a novel negative regulator of monoterpene biosynthesis. Ectopic expression of MsMYB, in sweet basil and tobacco, perturbed sesquiterpene- and diterpene-derived metabolite production. In addition, we found that MsMYB binds to cis-elements of MsGPPS.LSU and suppresses its expression. Phylogenetic analysis placed MsMYB in subgroup 7 of R2R3-MYBs whose members govern phenylpropanoid pathway and are regulated by miR858. Analysis of transgenic lines showed that MsMYB is more specific to terpene biosynthesis as it did not affect metabolites derived from phenylpropanoid pathway. Further, our results indicate that MsMYB is probably not regulated by miR858, like other members of subgroup 7. [ABSTRACT FROM AUTHOR]

• Fragility curve of masonry building due to induced earthquake by GPP activity. • Developing new concept called Comfortability curve as measure of resident's comfort. • Finding suitable IM of fragility curve of masonry building under GPP-induced event. • Risk assessment of masonry building under GPP earthquakes considering human comfort. This research is an attempt to estimate the seismic risk of geothermal power plants (GPPs) operation on the masonry buildings located in the vicinity of these infrastructures through a probabilistic framework. In this regard, a typical masonry building in Germany is considered. In the first part, the fragility and comfortability curves of this building are established. For this purpose, a set of 30 ground motions recorded due to GPPs activity are used for performing elaborated three-dimensional nonlinear incremental dynamic analysis to construct the fragility and comfortability curves. In the second part of the research work, by using the Monte-Carlo simulation technique and adopting the hazard model capable of simulating both seismological characteristics and ground motion intensities, the required number of probable hazard scenarios are generated for different ranges of GPPs injection flow rates. Afterward, the seismic risk imposed on the masonry building is evaluated by considering the developed fragility and comfortability curves. The outcomes highlight that the risk of facing structural damage is not significant; however, the disturbance of buildings' residents is highly probable and can lead to considerable seismic risk. In addition, in the close vicinity of the earthquake epicenter (less than 5 km), the estimated risk increases tangibly in comparison with the larger distances (more than 15 km). [ABSTRACT FROM AUTHOR]

Brittle failure of key-hole notches is investigated experimentally and theoretically under mixed mode I/II loading with negative mode I contributions. The key-hole notched Brazilian disk specimen made of the general-purpose Polystyrene is utilized for conducting the fracture experiments. To predict the experimental results, the key-hole notch maximum tangential stress and key-hole notch mean stress criteria are utilized. It is shown that both criteria could provide good predictions to the experimental results of the fracture load. Moreover, the key-hole notch mean stress criterion with the experimentally determined critical distance is shown to be the most accurate failure criterion. [ABSTRACT FROM AUTHOR]

To guide conservators in their decisions in active conservation of polystyrene materials, seven adhesives were tested before and after light ageing. The material was investigated by assessment of working properties, appearance, colour measurement, tensile testing, hardness measurement, assessment of break type, scanning electron microscope imaging, and assessment of reversibility. Based on a survey among conservators, the adhesives included were acrylates (Paraloid® B-72 in acetone: ethanol, or only ethanol, Paraloid® B-67 in isopropanol, Primal® AC 35, Acrifix® 116), epoxies (HXTAL®-NYL-1, Araldite® 2020) and one cyanoacrylate (Loctite® Super Attack Precision). Adhesives were tested on extruded sheets of transparent, general purpose polystyrene applied on joined edges and as an open layer. Damage to the plastic could be seen for Acrifix® 116 and Loctite® Super Attack Precision. The average break force sensitivity values indicate that the cyanoacrylate was weakened while Acrifix® 116 was strengthened after ageing. In general, the cyanoacrylate was the strongest and Paraloid® B-67 the weakest. Most adhesives showed yellowing after ageing apart from Acrifix® 116 and HXTAL®-NYL-1. The Paraloids, Primal® AC 35, and the epoxies were possible to remove from the plastic. [ABSTRACT FROM AUTHOR]

For a cracked solid under mixed Mode-I/II loading, the present investigation shows that six energy-based driving forces act respectively on the six subintervals around a crack tip, which controls the underlying fracture configurations, including complex crack branching. Using the geometrical modelling that describes multiple cracks initiation from a crack tip and a mixity parameter φ n , subinterval division for crack tip boundary to maximized the energy release rate has been proposed. This is an efficient way to reveal theoretically energy-based driving forces acting on the local boundaries around a crack tip, and to show that the number of the subintervals is no greater than six. Three windows are suggested, within which the crack multiple-branching is more likely to be triggered. Compact tension shear (CTS) specimens made from general-purpose Polystyrenes (GPPS) under mixed Mode-I/II loading for a given φ n are considered to investigate fracture behaviors. The typical experimental findings are consistent with some typical fracture behaviors predicted by the present modelling. The current theoretical and experimental investigations could be helpful to refresh the understanding of crack branching under quasi-static or dynamic mixed Mode-I/II loading. [ABSTRACT FROM AUTHOR]

Geranyl diphosphate synthase (GPPS) is a plastid localized enzyme that catalyzes the biosynthesis of Geranyl diphosphate (GPP), which is a universal precursor of monoterpenes. Wintersweet (Chimonanthus praecox L.), a famous deciduous flowering shrub with a strong floral scent character, could have GPPS-like homologs that are involved in monoterpenes biosynthesis, but it remains unclear. In the present study, five full-length GPPS and geranylgeranyl diphosphate synthases (GGPPS) genes were identified in the wintersweet transcriptome database. The isolated cDNAs showed high protein sequence similarity with the other plants GPPS and GGPPS. The phylogenetic analysis further classified these cDNAs into four distinct clades, representing heterodimeric GPPS small subunits (SSU1 and SSU2), homodimeric GPPS, and GGPPS. Analysis of temporal expression revealed that all genes have the highest transcript level at the full-open flower stage. From tissue-specific expression analysis, CpGPPS.SSU1 and CpGGPPS1 were predominantly expressed in petal and flower, whereas CpGPPS.SSU2, GPPS, and GGPPS2 showed a constitutive expression. Additionally, the subcellular localization assay identified the chloroplast localization of SSUs and GGPPSs proteins, and the yeast two-hybrid assay showed that both CpGPPS.SSU1 and CpGPPS.SSU2 can interact with the GGPPS proteins. Taken together, these preliminary results suggest that the heterodimeric GPPS can regulate floral scent biosynthesis in wintersweet flower. [ABSTRACT FROM AUTHOR]

Residual styrene present in polystyrene food packaging may migrate into food at low levels. To assure safe use, safe exposure levels are derived for consumers potentially exposed via food using No/Low Adverse Effect Levels from animal and human studies and assessment factors proposed by European organisations (EFSA, ECHA, ECETOC). Ototoxicity and developmental toxicity in rats and human ototoxicity and effects on colour discrimination have been identified as the most relevant toxicological properties for styrene health assessments. Safe exposure levels derived from animal studies with assessment factors of EFSA and ECHA were expectedly much lower than those using the ECETOC approach. Comparable safe exposure levels were obtained from human data with all sets of assessment factors while ototoxicity in rats led to major differences. The safe exposure levels finally selected based on criteria of science and health protection converged to the range of 90-120 mg/person/d. Assuming a consumption of 1 kg food/d for an adult, this translates to 90 mg styrene migration into 1 kg food as safe for consumers. This assessment supports a health based Specific Migration Limit of 90 ppm, a value somewhat higher than the current overall migration limit of 60 ppm in the European Union., (Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.)