15,701 results on '"GOAT"'
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2. Evaluation of alternative vaccination routes against paratuberculosis in goats.
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Criado, Miguel, Silva, Marta, Arteche-Villasol, Noive, Zapico, David, Elguezabal, Natalia, Molina, Elena, Espinosa, José, Ferreras, María del Carmen, Benavides, Julio, Pérez, Valentín, and Gutiérrez-Expósito, Daniel
- Abstract
Paratuberculosis is a chronic granulomatous enteritis, caused by Mycobacterium avium subspecies paratuberculosis (Map), that affects ruminants worldwide. Vaccination has been considered the most cost-effective method for the control of this disease in infected dairy herds. However, currently available vaccines do not provide complete protection and interfere with the diagnosis of both paratuberculosis and bovine tuberculosis, limiting its use. Because of that, efforts are being made for the development of new vaccines. The primary objective of this study was to evaluate the efficacy of two whole-cell inactivated experimental vaccines against paratuberculosis in goats, administered through the oral (OV) and intradermal (IDV) routes, and compare them with that of the commercial subcutaneous vaccine Gudair
® (SCV). Over an 11-month period, the effect of vaccination and a subsequent Map challenge on the specific peripheral immune responses and Map- DNA fecal shedding were recorded. At the end of the experiment, tissue bacterial load and lesion severity were assessed. The experimental vaccines did not induce specific humoral immune responses and only elicited mild and delayed cellular immune responses. Although the OV reduced lesion severity, neither this vaccine nor the IDV prototype was able to reduce fecal shedding or tissue bacterial load. Moreover, although the SCV did not confer sterile immunity, it outperformed both experimental vaccines in all these parameters. [ABSTRACT FROM AUTHOR]- Published
- 2024
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3. Molecular prevalence and genotype identification of Enterocytozoon bieneusi in cattle and goats from Zhejiang Province, China.
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Xin, Xianming, Sun, Lijie, Liu, Wei, Zhang, Jiayin, Ma, Shiyang, Fu, Xinyi, Zhao, Wei, and Yan, Baolong
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Introduction: Enterocytozoon bieneusi (E. bieneusi) is a widespread intracellular fungi that poses a significant zoonotic threat due to its infectivity toward both humans and animals. Methods: To evaluate the zoonotic transmission potential of this fungi, a molecular investigation was undertaken on E. bieneusi in cattle and goats reared across multiple cities in Zhejiang Province, China. A total of 651 fresh samples were collected, consisting of 265 cattle and 386 goats. The presence of E. bieneusi was determined by PCR amplification and sequencing analysis of the internal transcribed spacer (ITS) region of the small subunit ribosomal RNA (SSU rRNA) gene in all collected samples. Results: The results revealed that 17.1% (111/859) of the animals were afflicted with E. bieneusi , cattle having a prevalence of 14.0% (37/265) and goats displaying a higher rate of 19.2% (74/386). Seventeen E. bieneusi genotypes were identified, including 10 known, CHG5 (n = 30), CHG3 (n = 21), I (n = 14), J (n = 12), CHG2 (n = 11), COS-II (n = 8), D (n = 4), CHG19 (n = 2), ETMK5 (n = 1), and Henan III (n = 1), and seven novel, ZJG-I to ZJG-VI and ZJN-I (one each) genotypes. Discussion: These findings indicate widespread infection of E. bieneusi among the surveyed animals, thereby raising concerns about zoonotic genotypes that could pose potential threats to public health. Furthermore, the identification of novel genotypes of E. bieneusi offers valuable insights into the genetic diversity of this pathogen. [ABSTRACT FROM AUTHOR]
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- 2024
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4. The African introgression of Murciano Granadina goats has a Moroccan origin and displays remarkable levels of inter‐individual variability.
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Petretto, E., Luigi‐Sierra, M. G., Vacca, G. M., Martínez, A., Delgado, J. V., Fernández Álvarez, J., Castelló, A., Pazzola, M., Jordana, J., Dettori, M. L., and Amills, M.
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LIVESTOCK breeding , *LIVESTOCK breeds , *GOATS , *SUDANESE , *SAMPLE size (Statistics) - Abstract
There is evidence that Murciano Granadina (MG), the most important caprine dairy breed in Spain, has been introgressed by African goats, but the precise geographic origin of such introgression has not been identified yet. Moreover, an accurate estimate of the magnitude of this African introgression is lacking, since current estimates are based on small numbers of sampled individuals. The aim of our work was to tackle these two issues by genotyping 500 MG goats with the Goat SNP50 BeadChip and comparing their genotypes with those of reference populations from Spain (Bermeya), France (Saanen), Morocco (Barcha, Draa, Ghazalia, Noire de Atlas, Nord, Moroccan), Egypt (Barki, Oasis, Saidi), Algeria (Arabia, Makatia, M'Zabite, Kabyle), Tunisia (Tunisian native breeds) and Sudan (Desert, Nilotic, Taggar). The population of 500 MG goats was subdivided into 10 datasets of 50 individuals to ensure that sample sizes of the target (MG) and reference populations are balanced. Performance of an unsupervised ADMIXTURE analysis demonstrated that MG goats have a North African ancestry, with an average proportion of 4.4 ± 2.3%. Next, we did a supervised ADMIXTURE analysis that revealed that the Moroccan genetic component reaches a proportion of 4.01 ± 3.9% in MG goats, while the Algerian (0.001 ± 0.001%), Egyptian (0.2 ± 0.1%), Sudanese (0.1 ± 0.1%) and Tunisian (0.3 ± 0.4%) components are present in extremely small proportions. The historical circumstances of this introgression event are currently unknown, but several plausible scenarios are outlined. Moreover, our results show considerable inter‐individual heterogeneity regarding the magnitude of the Moroccan introgression of MG goats (0%– 12% depending on the MG data set under analysis). This result implies that reliable estimates about the introgression of autochthonous livestock by exotic breeds can only be obtained by extensively sampling target populations. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Effects of black soldier fly larvae (Hermetia illucens L.) as feed supplements on muscle nutrient composition, meat quality, and antioxidant capacity in Qianbei goat.
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Shengyong Lu, Siwaporn Paengkoum, Shengchang Chen, Yong Long, Xinran Niu, Sorasak Thongpea, Nittaya Taethaisong, Weerada Meethip, and Pramote Paengkoum
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HERMETIA illucens , *UNSATURATED fatty acids , *MEAT quality , *ONE-way analysis of variance , *OXIDANT status - Abstract
Objective: Black soldier fly (BSF) as an animal protein feed source is currently becoming a hot research topic. This study investigated the effects of the BSF as a protein feed source for goats on slaughter performance, muscle nutrient composition, amino acids, fatty acids, minerals, and antioxidant levels. Methods: Thirty Qianbei Ma goats (20.30±1.09 kg) were randomly divided into three groups: the control group (GRPC) supplemented with 10% full-fat soybean, treatment 1 (GRPU) supplemented with 10% untreated BSF, and treatment 2 (GRPT) supplemented with 10% heat-treated BSF. One-way analysis of variance among groups (with Fisher's least significant difference post hoc comparison) was used in this study. Results: The nutrients, amino acids, fatty acids, minerals, and antioxidants in muscle were analyzed. The results showed that there were no significant differences in the moisture, dry matter, crude protein, ash, amino acids, and mineral content of the muscles among the three feeding groups. The slaughter rate and carcass weight of the GRPU and GRPT groups were significantly lower (p<0.05). The overall meat quality of the GRPU and GRPT groups decreased (p<0.05). The individual unsaturated fatty acids and total unsaturated fatty acids in the GRPU group were higher (p<0.05) than those in the GRPC and GRPT groups. Both GRPU and GRPT decreased (p<0.05) the antioxidant capacity of the meat. Conclusion: Therefore, the heat-treated BSF had a better effect on meat quality compared to untreated BSF, but there were greater negative effects on the meat quality of GRPU and GRPT than GRPC. [ABSTRACT FROM AUTHOR]
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- 2024
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6. MOLIBDEN W ŻYWIENIU PRZEŻUWACZY.
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Mirowski, Adam
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Nutrition is one of the most important factors influencing health status and animal performance. Animal tissues contain low levels of mineral substances that play key roles in regulation of many biological processes. Nutritional deficiencies and excesses of mineral substances should be avoided. Excessive dietary intake of one element may cause deficiency of another element in the body. Molybdenum belongs to the substances that decrease copper bioavailability. Molybdenum supplementation reduces copper accumulation and decreases its concentration in the liver. The aim of this paper was to present the aspects connected with the importance of molybdenum in ruminant nutrition. [ABSTRACT FROM AUTHOR]
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- 2024
7. Assessment of feeding hydroponic fodder on growth and reproduction performance in Black Bengal goat.
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Rajak, Shailendra Kumar, Singh, Arvind Kumar, Singh, Abhishek Kumar, Rai, Asish, and Gangwar, Anshu
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This study aimed to evaluate the effects of hydroponic fodder on the growth and reproductive performance of Black Bengal goats in East Champaran, Bihar, during the years 2020-21 and 2021-22. A total of 24 goats were randomly assigned to four groups: Control (C) with extensive grazing, gram straw, concentrate feed, and green fodder; Treatment 1 (T1) receiving C + 300 g/day of wheat hydroponic fodder; Treatment 2 (T2) with 300 g/day of maize hydroponic fodder alone; and Treatment 3 (T3) receiving C + a combination of 150 g/day of wheat and 150 g/day of maize hydroponic fodder. Each group comprised 6 animals. The nutrient composition analysis revealed that wheat hydroponic fodder had significantly higher (P < 0.05) levels of dry matter, crude protein, and lignin, whereas maize contained higher nitrogen-free extract, crude fiber, ether extract, and ash. In terms of growth performance, T3 showed the highest body weight gain and daily weight gain, outperforming T2, T1, and the control group (P < 0.05). Though differences between T2 and T3 were not significant (P > 0.05) across both years, T3 consistently exhibited better performance. Reproductive performance indicators, such as age at puberty, age of first kidding, and kid mortality rates, were significantly lower (P < 0.05) in T3, T1, and the control group compared to T2. However, there were no significant differences (P > 0.05) between T2 and T3 in these parameters. T3 also showed the highest kidding size and birth weight of kids (P < 0.05), followed by T2, T1, and the control group. The cost of feed per kilogram of body weight gain was lowest in T3, followed by T2, T1, and the control. In conclusion, the combination of maize and wheat hydroponic fodder (T3) resulted in better growth and reproductive performance compared to feeding maize or wheat hydroponic fodder alone. Furthermore, this feeding strategy reduced the cost per kilogram of live weight gain, indicating its potential for enhancing the sustainability and profitability of goat farming systems in the region. [ABSTRACT FROM AUTHOR]
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- 2024
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8. 饲喂胆汁酸对亚急性瘤胃酸中毒山羊回肠黏膜形态、 菌群组成和 IFN-γ mRNA 相对表达量的影响.
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陈悦, 宋品, 侯曼曼, 杨笑然, 刘丽萍, and 倪迎冬
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Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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9. Molecular detection and genetic characterization of hemotropic mycoplasmas in goats and fleas from Thailand.
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Ghauri, Muhammad Shair Zaman, Soomro, Samiullah, Novianto, Dimas, Arnuphapprasert, Apinya, and Kaewthamasorn, Morakot
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Arthropod vectors play a crucial role in the transmission of hemotropic mycoplasmas, small bacteria that infect red blood cells in a wide range of animals and humans globally, leading to intravascular infections. Traditional Giemsa-stained thin blood smears, used for diagnosing hemotropic mycoplasmas through microscopic examination, have low sensitivity and are effective only when bacteremia levels are high. This study aimed to employ molecular methods to detect and genetically characterize hemotropic mycoplasmas in goats as well as investigate the potential role of fleas as vectors. Blood and flea samples were collected concurrently from goats on 16 farms across seven provinces in Thailand from January 2017 to October 2023. The 16 S rRNA, 23 S rRNA, and rnpB genes of hemoplasmas were amplified and sequenced. All fleas were identified morphologically and molecularly through DNA barcoding of the cytochrome oxidase I gene. A total of 78 out of 500 goats (15.6%), three pooled flea samples (3/6, 50%), and one individual flea (1/49, 2.04%) tested positive for hemoplasmas and all fleas were identified as Ctenocephalides orientis. BLASTN searches utilizing the three genetic markers revealed that the hemoplasmas detected in this study showed 97.81–100% similarity to Mycoplasma ovis and Candidatus Mycoplasma haemovis, which have been previously reported in sheep, goats, and humans, suggesting their zoonotic potential. The sequences were grouped into 28 unique nucleotide sequence types (ntSTs) based on minor variations in the 16 S rRNA gene. Hemotropic mycoplasma infection was significantly associated with farm locations and seasonality of sample collection (p < 0.0001), indicating that farm management practices or environmental conditions may play a critical role in the epidemiology of these infections. This study represents the first report of hemotropic mycoplasmas in goats in Thailand, confirms their presence in fleas, and provides valuable insights for farm management, such as guiding the rational use of insecticides and antibiotics. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Characterization and typification of small-scale goat production systems in the highlands of southeast Peru.
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Palomino Guerrera, Walter, Godoy Padilla, David, Huaripaucar Huancahuari, Joseen, Sessarego Dávila, Emmanuel, Trillo Zárate, Fritz, and Cruz Luis, Juancarlos
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GOAT breeds ,SUSTAINABLE agriculture ,CROP residues ,GOAT farming ,SURPLUS commodities ,GOAT milk - Abstract
Goat breeding in Peru is one of the main activities of smallholders. Goats are distributed in different agroecological zones and regions of the country, developing under heterogeneity of production systems, making it difficult to understand goat breeders' socioeconomic, technological, and productive situations. This study aimed to characterize and typify the goat production systems in the highlands of southeast Peru. A survey was conducted with 91 goat farmers from five districts of Ayacucho, Peru, using a structured and individualized questionnaire administered on their farms. The socio-economic, productive, and commercial characteristics of the goat production systems were recorded. A multiple correspondence analysis (MCA) and hierarchical classification analysis (HCA) were performed to establish a typology of the smallholders. The results reveal that the breeding system is extensive, where there is no breeding program, with natural pastures and crop stubble being the source of food for the herds. Only slightly more than half (54%) carry out a deworming program. Farmers were categorized into three different groups, corresponding to three different farming systems: Group 1 farmers raised goats solely for home consumption; Group 2 breeders raised goats for both consumption and marketing of surplus products (cheese, milk, and meat), and Group 3 farmed focused on producing cheese and goat kids and selling to local markets. The study provides valuable insights that will help design effective breeding strategies to develop sustainable goat farming in the region, considering different production systems and their respective socio-economic and trade dynamics. This classification will be essential for tailoring development programs to the specific needs of each group, promoting better use of resources, improving productivity, and enhancing the livelihoods of smallholder goat producers in Peru. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Seroprevalence and associated risk factors of peste des petits ruminants in sheep and goats in three districts of the Central Oromia Region, Ethiopia.
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Wendimu, Tilahun Guday, Dinbiso, Tegegn Dilbato, Lobago, Demeke Sibhatu, and Marami, Lencho Megersa
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PESTE des petits ruminants ,DISEASE risk factors ,ENZYME-linked immunosorbent assay ,RISK perception ,ANIMAL health - Abstract
Background: Peste des petits ruminants (PPR) is a viral disease that affects domestic and wild small ruminants and camels in Africa, the Middle East, and Asia. Following the successful eradication of rinderpest, the World Organization for Animal Health (WOAH) and the Food and Agriculture Organization (FAO) have undertaken to eradicate PPR by 2030. Regular surveillance and monitoring of the disease in various regions of Ethiopia are crucial to achieving this goal. This study aimed to estimate the prevalence of PPR, assess community awareness of PPR infection, and examine the associated risk factors of the disease in selected districts of the Central Oromia Region, Ethiopia. Method: The study collected 384 serum samples from 73 flocks containing 217 sheep and 167 goats using a multi-stage sampling technique. A competitive enzyme-linked immunosorbent assay (c-ELISA) was used to detect antibodies against the PPR virus. Additionally, a pre-tested questionnaire was used to gather information on community awareness and potential risk factors for PPRV infection in the study area. Results: The study found that the overall prevalence of PPR in flocks was 71.2% [95% confidence interval (CI): 59.4%−81.2%]. The prevalence of PPR at the animal level was 50% (95% CI: 44.9%−55.1%), with sheep having a prevalence of 54.4% (95% CI: 47.0%−60.6%) and goats having a prevalence of 44.3% (95% CI: 36.6%−52.2%). The study also found that districts, flock size, and agroecology were independent predictors of PPRV seropositivity in sheep, whereas districts, origin, and mixed species were independent predictors of PPRV seropositivity in goats. Conclusion: The study revealed a high prevalence of PPR in sheep and goats in the study area. To prevent the spread of the disease, the study suggests quarantining animals before introducing them to districts, regular PPR vaccination, and isolation and molecular characterization of the PPR virus circulating in the study area. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Efficacy of a polyherbal formula for controlling lice infestation in chickens and goats in backyard farming system in Romania.
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Coroian, Mircea, Hociotă, Cristinel-Cornel, Varga, Erzsébet, Fülöp, Ibolya, Fazakas, Mihaly, and Györke, Adriana
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PEDICULOSIS ,APHIDS ,HIGH performance liquid chromatography ,DOMESTIC animals ,ORGANIC farming - Abstract
Introduction: Lice infestation negatively impacts the welfare and productivity of domestic animals. This study aimed to evaluate the efficacy of a commercial polyherbal formula (PHF) containing Asarum europaeum , Lavandula angustifolia , Artemisia absinthium , and Tanacetum vulgare in managing lice infestations in chickens and goats within a backyard farming system in Romania. Methods: The PHF was analyzed using high-performance liquid chromatography (HPLC) to determine its phytochemical composition. Chickens and goats with confirmed lice infestations were treated with the PHF by spraying it on the entire body. Treatments were administered three times at 14-day intervals for goats and at both 14- and 7-day intervals for chickens. Lice infestations were assessed in experimental groups before each treatment by counting the collected lice specimens. Results: HPLC analysis identified key compounds in PHF: kaempferol (336.25 mg/ml), quercetin (88.38 mg/ml), epicatechin (43.98 mg/ml), and catechin (9.43 mg/ml). The PHF effectively controlled chewing lice in both chickens and goats, achieving a mortality rate of 76–98% in chickens and 96% in goats. However, the PHF was not effective against sucking lice in goats. A significant reduction in lice infestation (75% in chickens) was observed when the PHF was applied at 7-day intervals. No adverse reactions were noted in treated animals. Discussion: The polyherbal formula demonstrated promising efficacy against chewing lice in chickens and goats, supporting its potential use as an alternative to chemical treatments, especially in organic farming settings. The findings suggest that this PHF could be a viable option for managing lice infestations in domestic animals, particularly where organic practices are prioritized. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Establishment and preliminary application of PCR-RFLP genotyping method for Giardia duodenalis in goats.
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Mu, Xuanru, Guo, Jianchao, Wang, Hongcai, Li, Yilong, Yuan, Kaijian, Xu, Hui, Zeng, Wenjing, Li, Qiaoyu, Yu, Xingang, and Hong, Yang
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MIXED infections , *NUCLEIC acids , *SEQUENCE alignment , *SMALL intestine , *SEQUENCE analysis - Abstract
Background: Giardia duodenalis (G. duodenalis) is a globally distributed zoonotic protozoan that parasitizes the small intestines of humans and various mammals, such as goats and sheep. The objective of this study was to establish a convenient, accurate, and specific method based on restriction fragment length polymorphism (RFLP) for genotyping assemblages A, B and E of G. duodenalis in goats. The β-giardin gene was amplified using primer pairs bgF1, bgR1, bgF2 and bgR2 by nested PCR. The PCR products were digested with the restriction enzymes Hinf I and Bgl I. The established PCR-RFLP method was used to detect and analyze the genetic subtypes of G. duodenalis in 130 fecal samples from goats and compared simultaneously with microscopic examination and nucleic acid sequencing for G. duodenalis. Results: Genetic sequencing confirmed that the PCR-RFLP method accurately distinguished G. duodenalis assemblages A, B and E, as well as different combinations of mixed infections of these three assemblages. Among the 130 samples tested by PCR-RFLP, a total of 26 samples (20.00%) tested positive for G. duodenalis, a higher sensitivity than microscopic examination at 13.85% (18/130). Sequence alignment analysis revealed that among the 26 PCR-positive samples, two were identified as assemblage AI, while the remaining 24 were identified as assemblage E or E12. Conclusions: This study established an accurate, efficient and rapid PCR-RFLP genotyping method using the bg sequence of G. duodenalis, enabling accurate identification and effective differentiation of goat-derived G. duodenalis assemblages without requiring sequencing. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Assessment of Babesia ovis pathogenicity in goats: implications for transmission dynamics and host resistant.
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Ozubek, Sezayi, Ulucesme, Mehmet Can, Suarez, Carlos E., Bastos, Reginaldo G., and Aktas, Munir
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HEALTH of sheep ,INFECTIOUS disease transmission ,GOATS ,SYMPTOMS ,RHIPICEPHALUS ,BABESIA - Abstract
Babesia ovis , commonly associated with ovine babesiosis, poses a significant threat to sheep health, often resulting in severe clinical manifestations and high mortality rates. However, the impact of B. ovis on goats has remained uncertain, prompting us to investigate its pathogenicity in caprine hosts. Experimental infections using B. ovis -infected blood inoculation and infected tick infestation, were conducted on spleen-intact (n=5) and splenectomized (n=5) goats. The experimental infection was performed using fresh blood obtained from a B. ovis -infected splenectomized sheep. One spleen-intact sheep served as a control for the experimental infection with B. ovis -infected Rhipicephalus bursa ticks. While all experimentally infected sheep (#501, #575) displayed severe clinical symptoms and high parasitemia, goats exhibited resistance, showing no significant clinical manifestations or sustained parasitemia. Notably, B. ovis was detected in two spleen-intact goats via nested PCR, prompting further investigation into their role as reservoirs for tick-borne transmission. These goats were then infested with Babesia spp.-free R. bursa larvae (0.1 gr) and adults (50 females and 50 males) for transstadial and transovarial transmission experiments respectively. Results indicated that chronically B. ovis -infected spleen-intact goats are not significant sources for maintaining the tick-borne transmission cycle of the parasite. These findings highlight the differential susceptibility of goats to B. ovis infection compared to sheep and their limited role as reservoirs for parasite transmission. Understanding the role of goats in B. ovis transmission and their resistance mechanisms can inform effective control measures and reduce economic losses in affected regions. Further research into caprine babesiosis and host immunological responses is essential to fully elucidate their possible role as reservoirs of the parasite, and underlying mechanisms of host susceptibility and parasite pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Changes in the growth performance, serum biochemistry, rumen fermentation, rumen microbiota community, and intestinal development in weaned goats during rumen-protected methionine treatment.
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Wang, Youli, Liu, Wei, Li, An, Qubi, Wuqie, Gong, Chengsi, Li, Xuening, Xing, Jiani, Shi, Haitao, Li, Yanyan, Wang, Yong, and Lin, Yaqiu
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SULFATE-reducing bacteria ,BLOOD urea nitrogen ,MICROBIAL communities ,SMALL intestine ,BUTYRIC acid - Abstract
Rumen-protected methionine (RPM) such as coated methionine (CM) and 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester (HMBi) was usually used in dairy cows, but how RPM affects meat goats remains unclear. In this study, thirty weaned male Jianzhou Da'er goats were randomly assigned to one of three treatments: fed basal diet or basal diet supplemented with 0.12% CM or 0.22% HMBi, with the aim of examining their impact on growth performance, serum biochemistry, rumen fermentation, rumen microbiota, and intestinal development in meat goats. The findings indicate that HMBi supplementation led to an increase in body weight, feed intake, and feed-to-gain ratio, whereas CM only resulted in an increase in feed intake (all p < 0.05). Both CM and HMBi resulted in an increase in serum total cholesterol (TC), blood urea nitrogen (BUN), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), albeit with a decrease in serum triglycerides (TG) and β -hydroxybutyric acid (BHB, all p < 0.05). Both CM and HMBi supplementation decreased the rumen butyric acid concentration (both p < 0.05). The 16S rRNA sequencing showed that HMBi supplementation significantly increased the total abundance of Bacteroidetes and Firmicutes. Both CM and HMBi supplements increased the abundance of Rikenella and Proteiniphilum but decreased the abundance of Eisenbergiella , Enterocloster , Massilioclostridium , Eubacterium , Angelakisella , Blastopirellula , Christensenella, and Pseudoruminococcus. CM supplementation specifically increased the abundance of Desulfobulbus , Sodaliphilus, and Coprococcus while decreasing the prevalence of Anaerocella , Mogibacterium, and Collinsella. The supplementation of HMBi significantly enhanced the abundance of Paraprevotella , Bacilliculturomica , Lachnoclostridium , Dysosmobacter , Barnesiella , and Paludibacter , while decreasing the abundance of Butyrivibrio and Pirellula. Moreover, the administration of both CM and HMBi supplementation resulted in an increase in the ammonia-producing and sulfate-reducing bacteria, whereas a decrease was observed in the ammonia-oxidating, health-associated, and disease-associated bacteria. Correlational analysis revealed that TG and BHB had a positive correlation with disease-associated and ammonia-oxidating bacteria, whereas they had a negative correlation with ammonia-producing bacteria. The serum BUN, ALP, and AST were positively correlated with ammonia-producing bacteria but were negatively correlated with ammonia-oxidating bacteria. Furthermore, both CM and HMBi supplementation improve the development of the small intestine, with HMBi having a better effect. In summary, this study indicates that both CM and HMBi supplementation improve lipid metabolism, nitrogen utilization, and intestinal development. The growth promotion effect of HMBi supplementation may be attributed to the increased abundance of volatile fatty acid-producing and nitrogen-utilizing bacteria and improved intestinal development. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Investigation of the heat shock protein 70 activity in intestine cells of goats with coccidiosis.
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Hesapçıoğlu, Mehmet and Avcı, Hamdi
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Coccidiosis is one of the most common infectious diseases in goat farming. The disease causes major economic loss in the world. In this study, we aimed to investigate the activity of heat shock protein 70 in intestine cells of goats with coccidiosis. We used total of twenty-seven goats for this purpose. Gross findings were diarrhoea, cachexia, and dehydration. In the microscopical examination, we observed proliferative enteritis with
Eimeria . parasites. Immunohistochemical examinations revealed moderate to severe Hsp70 immunoreactivity in intestines. Considering Hsp70 is a stress protein with anti-apoptotic and immune regulatory features, Hsp70 immunoreactivity attributed to the stress caused by infection and anti-apoptotic activity of the protein along with immune regulatory effects of Hsp70. [ABSTRACT FROM AUTHOR]- Published
- 2024
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17. Identification of Goat Supernumerary Teat Phenotype Using Wide-Genomic Copy Number Variants.
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Xu, Lu, Zhang, Weiyi, Zhang, Haoyuan, Yang, Xiuqin, Ceccobelli, Simone, Zhao, Yongju, and E, Guangxin
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DNA copy number variations , *SEROTONIN receptors , *RNA-binding proteins , *MAMMARY glands , *BREAST cancer , *IMMUNOGLOBULIN receptors - Abstract
Simple Summary: Supernumerary teats (SNTs) refer to developmental anomalies that occur during the embryonic period and are commonly found around the mammary line. We performed a genome-wide selective sweep analysis on 37 goats using copy number variants, and identified multiple genes involved in embryonic mammary gland development and the biological process of breast cancer. Our findings can be used as a reference for the further investigation of SNT phenotypic candidate genes. Supernumerary teats (SNTs) or nipples often emerge around the mammary line. This study performed a genome-wide selective sweep analysis (GWS) at the copy number variant (CNV) level using two selected signal calculation methods (VST and FST) to identify candidate genes associated with SNTs in goats. A total of 12,310 CNVs were identified from 37 animals and 123 CNVs, with the top 1% VST values including 84 candidate genes (CDGs). Of these CDGs, minichromosome maintenance complex component 3, ectodysplasin A receptor associated via death domain, and cullin 5 demonstrated functions closely related to mammary gland development. In addition, 123 CNVs with the top 1% FST values were annotated to 97 CDGs. 5-Hydroxytryptamine receptor 2A, CCAAT/enhancer-binding protein alpha, and the polymeric immunoglobulin receptor affect colostrum secretion through multiple signaling pathways. Two genes, namely, RNA-binding motif protein 46 and β-1,3-galactosyltransferase 5, showed a close relation to mammary gland development. Six CNVs were identified and annotated to five genes by intersecting the top 1% of candidate CNVs with both parameters. These genes include LOC102185621, LOC102190481, and UDP-glucose pyrophosphorylase 2, which potentially affect the occurrence of BC through multiple biological processes, such as cell detoxification, glycogen synthesis, and phospholipid metabolism. In conclusion, we discovered numerous genes related to mammary development and breast cancer (BC) through a GWS, which suggests the mechanism of SNTs in goats and a certain association between mammary cancer and SNTs. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Heat Stress Impairs Endometrial Function During Implantation by Regulating Autophagy in Hainan Black Goat.
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Li, Xiaoping, Sun, Yanyu, Min, Yi, Wang, Xinyu, Yang, Diqi, and Peng, Hui
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ANIMAL health , *EMBRYO implantation , *PROTEIN overexpression , *TIGHT junctions , *FACTORIAL experiment designs , *ENDOMETRIUM - Abstract
Simple Summary: Heat stress (HS) negatively affects the health and productivity of domestic animals, particularly influencing embryo implantation rates. This study aims to understand the effects of heat stress on endometrial function in Hainan black goats during the peri-implantation period. We collected uterine tissue samples from both control and heat-stressed goats and conducted experiments under two temperature conditions: normal (37 °C) and heat stress (42 °C), along with two pharmacological treatments—chloroquine (CQ) and rapamycin (RAPA). Our results indicate that heat stress initially suppresses autophagy activity, which increases with prolonged exposure. Modulating autophagy through these treatments altered the expression of endometrial receptivity markers. Notably, the overexpression of a specific protein partially reversed the downregulation caused by heat stress. Additionally, a tight-junction protein was degraded under certain conditions but accumulated when cells were treated with CQ. These findings suggest that autophagy plays a protective role in maintaining endometrial function during heat stress, offering insights that may help improve reproductive outcomes in goats facing heat challenges Heat stress (HS) is a significant factor that adversely affects the health, welfare, and productivity of domestic animals, particularly impacting embryo implantation rates. However, the effects of HS on endometrial function during the peri-implantation period in Hainan black goats remain unclear. This study explores the influence of HS on the endometrium of these goats. We collected uterine tissue samples from both control and heat-stressed goats and performed in vitro experiments using a 2 × 2 factorial design. This design included two temperature conditions (37 °C as the control and 42 °C to simulate heat stress) and two pharmacological treatments: chloroquine (CQ), an autophagy inhibitor, and rapamycin (RAPA), an autophagy activator. Our results showed that heat stress initially suppresses autophagy activity, which is subsequently enhanced with prolonged exposure. The pharmacologic modulation of autophagy, through activation or inhibition, resulted in corresponding upregulation or downregulation of the endometrial epithelial cells' (EECs) receptivity markers. The overexpression of ATG7 partially reversed the HS-induced downregulation of these markers. Additionally, TJP1, a tight-junction marker, was degraded under the pharmacologic and genetic activation of autophagy in HS conditions but accumulated more in the EECs pre-treated with CQ. These findings suggest that autophagy plays a protective role in maintaining endometrial function under HS conditions in Hainan black goats. This study offers valuable insights into the role of autophagy in endometrial receptivity and proposes a potential strategy to mitigate the adverse effects of HS on goat reproduction. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Comparative study on slaughter performance, meat quality, and rumen microbiota of Hainan Dong goat and its hybrid with Nubian goat.
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Shi, Huiyu, Zhang, Naifeng, Tu, Yan, Yun, Yanhong, Diao, Qiyu, and Ma, Tao
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EYE muscles ,MEAT quality ,HOT weather conditions ,GOAT meat ,MICROBIAL communities - Abstract
Introduction: The Hainan Dong goat (DG) is a local meat breed widely raised in Hainan, China because of its good adaptability to local hot and humid weather. However, the growth rate of these DG is much slower than that of commercialised breeds improved in European countries, resulting in poor carcase characteristics, including smaller slaughter weight and carcase weight, which have become increasingly prominent. In recent decades, Nubian goats have been continuously imported into China to improve the production performance of local breeds. Methods: In this study, the effects of breed on growth performance, carcase and meat quality, and ruminal microbiota were analysed in 40 goats, including 20 DGs and 20 hybrid F3 offspring generated by crossing the DG and Nubian hybrids (NH). All the goats were averagely aged 90 days and weighed at 11 ± 1.34 kg. They were assigned to two treatments, with three replicates per treatment. The two groups were fed the same diet for 90 days before slaughter. Results: The results showed that the average daily gain, F/G ratio, slaughter weight, and carcase weight of the NH group were higher than those of the DG group (p < 0.05). However, tube circumference, meat-to-bone ratio, and eye muscle pH were lower in the NH group than in the DG group (p < 0.05). The NH group showed a smaller fibre crosssectional area and fibre diameter, but a larger fibre density than the DG group (p < 0.05). Bacteroidetes and Firmicutes were the most dominant phyla in the two groups; however, the two breeds had different ruminal microbial communities. Discussion: In the present study, the differences in growth performance between two groups of goats under the same feeding environment and feed conditions were compared. The correlation between feed sources and rumen flora has been demonstrated, and the results of this study show that the same diet has similar effects on rumen microorganisms, which in turn have related effects on growth and production performance. Conclusion: In summary, hybrids can improve the growth and slaughter performance of local breeds, which may be related to changes in the rumen microorganisms. This study revealed that crossbreeding of Nubian goats with Dong goats has the potential to be used in a wide range of applications owing to its effectiveness in increasing production efficiency. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Familial Congenital Hyperplastic and Colloidal Goitre in a Beetle Goat.
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Ravindran R., Bassessar, Varun, Syal, Priyanka, and Punia, Sunil
- Abstract
An investigation was conducted at the Veterinary Clinical Complex, College of Veterinary Science, COVS, GADVASU Rampura Phul, Punjab, India during May, 2023 to study the pathomorphological alterations of Hyperplastic and Colloidal Goiter in a beetal. Two new born kids were considered that included one dead and other with history of no suckling, lassitude, not able to rise its head and swelling in cranioventral neck region. Post mortem examination of dead kid showed markedly swollen thyroid gland and samples collected from them were subjected to routine H&E examination. Histopathological examination revealed extensive hyperplasia of the thyroid follicles along with follicles filled with varying amount of colloid suggesting hyperplastic and colloidal goitre. Areas of Hyperplastic follicles showed cuboidal to tall columnar follicular epithelial cells with highly vacoular cytoplasm whereas follicles with colloidal deposition showed flattened epithelium. Hemato-biochemical examination of samples collected from live kid showed moderate anaemia with decreased leucocyte count. There was macrocytic hypochromic anaemia. T4 was significantly lower and there was significant rise in TSH levels when compared to reference values. The kid born co-twin with the dead kid survived after treatment and showed significant improvement in clinical condition after 5 days of treatment. Based on the gross and histopathological examination the cause of death was confirmed to be congenital hyperplastic and colloid goitre which is usually a non-inflammatory and non-neoplastic enlargement of the thyroid caused due to iodine deficiency is most common in new-born animals in iodine-deficient areas. [ABSTRACT FROM AUTHOR]
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- 2024
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21. Impact of a natural rubber-based scratcher as an environmental enrichment on the scratching behavior, cortisol level, and semen quality of stable male goats.
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Kimsakulvech, Sakdichod, Sriphavatsarakom, Prarom, Thaikoed, Sunsaneeya, Oupala, Waraluk, Punkong, Chainarong, Prompiram, Phirom, Saingkaew, Somchai, Hayakijkosol, Orachun, and Tuempong Wongtawan
- Abstract
Background and Aim: Goats are valuable livestock because they can generate meat and milk for human consumption. Goat husbandry is becoming more intensive due to the growing demand for goat products, which may impact animal welfare and natural behavior. This study aimed to investigate the impact of natural rubber (para rubber)-based scratchers as an environmental enrichment on scratching behavior, cortisol levels, and semen quality in stable bucks (male goats/goats). Materials and Methods: Nine male goats were used in this study. Scratching behavior and cortisol levels were used as welfare indicators, whereas semen quality was evaluated as an indicator of reproductive potential. These indicators were analyzed before and after scratcher installation. Results: After installing the scratchers, the goats showed a significant increase in scratching behavior and a notable decrease in cortisol levels (p < 0.001). Notably, the goats exhibited a marked preference for scratching against the scratcher (p < 0.001) compared to the stable. They significantly preferred using their heads for scratching (p < 0.001) instead of other body parts. In addition, goats preferred to scratch on the softest rubber scratchers at specific installation locations (p < 0.001). Although there was a slight improvement in semen quality, there was no statistically significant difference (p > 0.05). Conclusion: A natural rubber-based scratcher can increase scratching behavior and reduce cortisol levels, indicating its potential to improve the welfare of farm goats. Selecting an appropriate hardness and preferred location is essential to ensure that the scratcher effectively encourages animals to use it. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Determination of Species‐Specific Differences in Intracranial Volume of Tuj Sheep and Hair Goats Using Stereology and Computed Tomography Methods.
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Koçyiğit, Ali, Kanik, Betül, Demircioğlu, İsmail, and Demiraslan, Yasin
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COMPUTED tomography , *SHEEP , *ANIMAL species , *STEREOLOGY , *GOATS - Abstract
The intracranial cavity contains vitally important organs. The brain, cerebellum, meninges and the vessels that supply these organs are located in the intracranial cavity. Therefore, it is important to learn about the intracranial cavity and to study it. However, there is limited information about the intracranial cavity in the veterinary field. The aim of this study was to determine the differences between the intracranial cavities of different species of animals by using stereology and tomography methods, volume calculations and morphometric measurements. In addition, the compatibility of the methods used with each other was investigated. In the study, six male adult goats and six male adult sheep were used. In this study, the intracranial cavities of sheep and goats were calculated by using Cavalieri's principle and 3D modelling using tomography sections. Morphometric measurements were taken over the intracranial space, and index calculations were made. In 3D models using computed tomography, the intracranial volume was 153.31 ± 24.06 cm3 in goats and 128.07 ± 7.93 cm3 in sheep. In the calculation using Cavalieri's principle, it was determined to be 152.73 ± 22.73 cm3 in goats and 126.15 ± 8.38 cm3 in sheep. As a result of the study, the MWCC (maximum width of the cranial cavity) parameter was found to be statistically significant between species (p < 0.05). The two methods used in Bland‐Altman analysis were found to be within the limits of agreement, and the methods can be alternative to each other. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Effects of Different Technological Forms of the Perilla frutescens in the Diet on Ruminal Fermentation, Milk and Plasma Fatty Acid Composition, Ruminal Biohydrogenation and Milk Quality in Dairy Goats.
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Beyzi, Selma Büyükkılıç, Ülger, İsmail, Kaya, Emrah, Ocak, Buğra, and Konca, Yusuf
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PERILLA frutescens , *MILKFAT , *UNSATURATED fatty acids , *MILK quality , *GOATS , *CHOLESTEROL content of food , *GOAT milk - Abstract
Background: Fatty acids can be protected by changing their structure or form against microbial activity, and the different forms of fatty acids can modulate the ruminal biohydrogenation rate and contribute to the desired fatty acid profile in milk fat. Objectives: The study investigated the effects of perilla (Perilla frutescens) dietary supplementation in the diet in different technological forms (seed, oil and formaldehyde‐treated oil) on milk, plasma and ruminal fatty acid composition, and milk quality in lactating goats. Methods: The four dietary treatments consisted of (1) no supplementation, basal diet (CON); (2) perilla supplementation as seed at 44.7 g/kg (consisting of 20 g/kg oil (PS)); (3) perilla supplementation as oil at 20 g/kg (PO); (4) perilla supplementation as formaldehyde treated oil at 20 g/kg (protected perilla oil [PPO]). The experiment was implemented in a double 4 × 4 Latin square trial design, and sampling was carried out for 7 days after 21 days of adaptation. Results: Performance parameters were not affected by P. frutescens supplementation to the diet. PO decreased milk fat, whereas PPO increased milk fat. Milk cholesterol was not affected by P. frutescens dietary supplementation. Perilla oil supplementation in different forms to the diet did not affect ruminal pH, VFA and methane production. Perilla oil supplementation in different forms to the diet did not also affect the concentration of blood serum glucose, cholesterol and non‐esterified fatty acids. Perilla supplementation to the diet increased the milk conjugated linoleic acid (CLA), C18:3n‐3, C22:5n‐3, C20:5n‐3, C22:6n‐3 and polyunsaturated fatty acid (PUFA) concentrations, and PPO group showed the greatest values. Ruminal palmitic (C16:0) acid was decreased, and in perilla groups, stearic acid (C18:0) concentration had the lowest, and ruminal c‐9, t‐11 CLA concentration had the highest value in PPO. Conclusions: It has been found that the most effective form of perilla oil in increasing milk quality is that with formaldehyde treatment (protected form). Perilla oil, which is a rich source of omega 3 in the diet, can be used to increase milk quality in goats without adversely affecting performance, ruminal fermentation and blood parameters. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Morphologic Examination of Ossicula Auditus in Different Ruminants.
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Demircioğlu, İsmail and Aksünger Karaavci, Funda
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EAR ossicles , *MIDDLE ear , *COMPARATIVE anatomy , *GAZELLES , *RUMINANTS - Abstract
Background: The ossicula auditus is the structure that transmits vibrations from the tympanic cavity to the auris interna. This assembly is formed by three ossicles: the malleus, incus and stapes. In addition, the bone that exists as os lenticulare in humans exists as processus lenticulare in most mammals. Objective: To our knowledge, no in‐depth study has been published on the ossicula auditus of gazelles. This study aims to provide a comparative study of the morphology of the auditory ossicles in sheep, goats and gazelles. Methods: Ossicula auditus from six adult Awassi sheep, six hair goats and six gazelles was examined and photographed under a stereomicroscope. Results and conclusion: It was determined that the ossicula auditus of gazelle, hair goat and Awassi sheep consisted of malleus, incus and stapes. In the species examined, it was observed that the articular surface on the caput mallei, the malleus protrusions and the distal part of the manubrium mallei differed between species. It was determined that the incus bone consists of a body and two crus. As an important morphological difference, the proc. lenticulare was found to be on the crus breve in all three species. It was determined that the stapes bone was quadrangular in the species examined, especially in goats, but this quadrangular shape, which is specific to ruminants, was not fully formed in other species. As a result, it was determined that the ossicula auditus of gazelle, hair goat and Awassi sheep showed morphologically different characteristics. [ABSTRACT FROM AUTHOR]
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- 2024
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25. Neuromedin S regulates goat ovarian granulosa cell proliferation and steroidogenesis via endoplasmic reticulum Ca2+‐YAP1‐ATF4‐c‐Jun pathway.
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Sun, Xuan, Xia, Rongxin, Wang, Yifei, Wang, Feng, Liu, Zhipeng, Xue, Gang, and Zhang, Guomin
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STEROIDOGENIC acute regulatory protein , *TRANSCRIPTION factors , *GRANULOSA cells , *ENDOPLASMIC reticulum , *CYTOCHROME P-450 , *ESTROGEN receptors , *STEROID receptors - Abstract
Neuromedin S (NMS) plays key roles in reproductive regulation, while its function and mechanism in follicular development remain unclear. The current study aims to investigate the specific role and mechanisms of NMS and its receptors in regulating the proliferation and steroidogenesis of ovarian granulosa cells (GCs). Phenotypically, a certain concentration of NMS addition promoted the proliferation and estrogen production of goat GCs, accompanied by an increase in the G1/S cell population and upregulation of the expression levels of cyclin D1, cyclin dependent kinase 6, steroidogenic acute regulatory protein, cytochrome P450, family 11, subfamily A, polypeptide 1, 3beta‐hydroxysteroid dehydrogenase, and cytochrome P450, family 11, subfamily A, polypeptide 1, while the effects of NMS treatment were effectively hindered by knockdown of neuromedin U receptor type 2 (NMUR2). Mechanistically, activation of NMUR2 with NMS maintained endoplasmic reticulum (ER) calcium (Ca2+) homeostasis by triggering the PLCG1‐IP3R pathway, which helped preserve ER morphology, sustained an appropriate level of endoplasmic reticulum unfolded protein response (UPRer), and suppressed the nuclear translocation of activating transcription factor 4. Moreover, NMS maintained intracellular Ca2+ homeostasis to activate the calmodulin 1‐large tumor suppressor kinase 1 pathway, ultimately orchestrating the regulation of goat GC proliferation and estrogen production through the Yes1 associated transcriptional regulator‐ATF4‐c‐Jun pathway. Crucially, the effects of NMS were mitigated by concurrent knockdown of the NMUR2 gene. Collectively, these data suggest that activation of NMUR2 by NMS enhances cell proliferation and estrogen production in goat GCs through modulating the ER and intracellular Ca2+ homeostasis, leading to activation of the YAP1‐ATF4‐c‐Jun pathway. These findings offer valuable insights into the regulatory mechanisms involved in follicular growth and development, providing a novel perspective for future research. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Pharmacokinetics of nalbuphine administered intravenously and subcutaneously in goats (Capra aegagrus hircus).
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Garcia, Jessica D., Smith, Joe S., Minich, David, Hopson, Makenna, Rahn, Rebecca, Hampton, Chiara, Graves, Meggan, Bussières, Geneviève, Mulon, Pierre‐Yves, Ebner, Lisa S., and Cox, Sherry
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REVERSE phase liquid chromatography , *GOATS , *NALBUPHINE , *PHARMACOKINETICS , *PAIN management - Abstract
The purpose of this study was to evaluate the pharmacokinetics (PK) of intravenously (IV) and subcutaneously (SC) administered nalbuphine in domestic goats. Nalbuphine hydrochloride was administered at 0.8 mg/kg for both IV and SC routes in six goats with a minimum of 10‐day washout period between sample collection phases. Eighteen plasma samples were collected over a 36‐hour period, analyzed using reverse phase high‐performance liquid chromatography (HPLC). Plasma data were analyzed using compartmental and noncompartmental approaches. Following IV nalbuphine administration, elimination half‐life, area under the plasma concentration time curve from time 0 to infinity (AUC0 − ∞), concentration at time zero (C0), and total body clearance were 120.4 ± 39.1 (min−1 ± SD), 17311.01 ± 7227.32 (min·ng·mL−1 ± SD), 675.6 ± 337.13 (ng·mL−1 ± SD), and 44.5 ± 13.8 (mL·min−1·kg−1 ± SD), respectively. After SC nalbuphine administration, elimination half‐life, area under the plasma concentration time curve from time 0 to infinity (AUC0 − ∞), and maximum plasma drug concentration were 129 ± 52.9 (min−1 ± SD), 20826.5 ± 14376.2 (min·ng·mL−1), and 368.03 ± 503.78 (ng·mL−1). Calculated bioavailability for the SC route was 138 ± 126 (% ± SD). Nalbuphine in goats is characterized by rapid elimination and high subcutaneous bioavailability and may be a safe analgesic opioid option in goats in the future. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Effect of D,L methionine and ammonium chloride on urine acidification, urinary fractional excretion of calcium, and blood bicarbonate in clinically healthy goats.
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Neal, Casey E., Grissett, Gretchen P., Fleming, Sherrill, Wills, Robert W., and Woolums, Amelia R.
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AMMONIUM chloride , *TREATMENT effectiveness , *LEAST squares , *URINARY calculi , *EXCRETION - Abstract
BACKGROUND: Urinary acidification with ammonium chloride (AC) for urolith dissolution is a common treatment for goats with urolithiasis. Studies have reported increased fractional excretion of calcium (FECa) following AC administration, which could increase calcium-based urolithiasis. D,L methionine (MET) may result in similar acidification with less calcium excretion. OBJECTIVE To compare the effects of orally administered MET and AC on urine and blood pH, FECa, and blood HCO3- concentrations in male goats. METHODS: Prospective, randomized, crossover study. 12 healthy, 5-to-6-month-old Boer-cross wethers were administered 200 mg/kg of AC or MET orally for 14 days with a 7-day washout period between trials. Venous blood and urine samples were collected every 2 days. The effects of treatment and treatment day on urine and blood pH, HCO3-, and FECa were assessed using linear mixed models. RESULTS: Ammonium chloride and MET lowered least squares means (LSM) urine pH on day 6 (LSM, 7.49; 95% CI, 6.44 to 8.54), 8 (LSM, 7.78; 95% CI, 6.73 to 8.83), and 10 (LSM, 7.53; 95% CI, 6.49 to 8.58) when compared to day 0 (LSM, 8.23; 95% CI, 7.18 to 9.28). Some goats' urine indicated acidification (pH < 7.0) in the first phase of the trial; however, for the entire trial, a significant treatment effect was not detected on urine pH, blood pH, blood HCO3- or log10 FECa. CLINICAL RELEVANCE: Ammonium chloride and MET acidified urine of some goats. Dietary cation-anion difference should be considered when treating healthy goats to acidify their urine. [ABSTRACT FROM AUTHOR]
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- 2024
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28. Prevalence and risk factors of subclinical mastitis in goats in western Algeria.
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Belabdi, I., Amine Bekara, M. El, Djebbar, A., Sebaihia, M., Issad, N. Ait, and Mimoune, N.
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DISEASE risk factors ,MASTITIS ,GOATS ,MILK quality ,MILK yield - Abstract
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- 2024
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29. Effects of drinking saline water on carcass traits and meat quality of growing and mature Blackhead Ogaden sheep and Somali goats.
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Abera, Fitsum, Urge, Mengistu, Yirga, Hirut, and Yousuf, Yishak
- Abstract
Water salinity has a significant impact on water quality, posing challenges for livestock production and productivity, particularly in arid regions where climate change affects freshwater availability. This study aimed to determine the effect of drinking saline water on the carcass and meat quality traits of sheep and goats in Ethiopia. A total of 100 males with an average initial body weight of growing (18.17 ± 0.51) and mature (22.22 ± 0.52 kg) Blackhead Ogaden sheep and growing (17.99 ± 0.50) and mature (21.99 ± 0.54) kg) Somali goats were used. The design of the experiment was a three-way factorial RCBD with three-factor combinations (5 treatment levels, 2 species, and 2 age groups).Water treatments were natural water (Lake Basaka water (control), low saline water (L-SW), moderate saline water (M-SW), high saline water (H-SW), and very high saline water (VH-SW); that is, NaCl was added to natural water at concentrations of 7.95, 11.93, 15.90, and 19.88 g TDS/L, respectively. The finding showed that increasing salinity levels in drinking water reduced slaughter body weight (SBW), carcass weight (CW), dressing percentage (DP), rib eye area (RAE), total edible components (TEC), and increased total non-edible components (TNEC) (P < 0.05). Similarly, sheep and mature animals had higher (P < 0.001) SBW, CW, DP, RAE, and TEC than goats and growing animals. Sensory evaluation, shear force, and proximate analysis were affected by water salinity, species, and age groups (P < 0.05). Overall, the study revealed that consuming saline water above 11 g TDS/L affected carcass traits and meat quality in Somali goats and Blackhead Ogaden sheep. [ABSTRACT FROM AUTHOR]
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- 2024
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30. Genomic profiling of selective sweeps through haplotype differentiation unravelled genes associated with production and reproduction traits in Indian goat breeds.
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Thomas, Marykutty, G., Radhika, V., R. Thirupathy, and V., Aravindakshan T.
- Abstract
A comprehensive genomic scan of selective sweeps was conducted in autochthonous Attappady Black and improved dual-purpose Malabari goat breeds in south India. High-throughput single nucleotide polymorphism (SNP) marker data, obtained through Illumina goat SNP50 BeadChip genotyping of 48 goats (24 each of Attappady Black and Malabari goats), were utilized for the analysis. Selection signature analysis, employing hapFLK analysis based on haplotype differentiation, identified seven significant sweep regions (p < 0.005). Notably, one of these regions encompassed the genomic area housing the casein cluster and quantitative trait loci associated with milk production on chromosome 6. Gene ontology enrichment analysis of 166 putative selective genes associated with these sweep regions revealed 13 significantly over-represented Panther pathways (p ≤ 0.05), including the TGF-beta signalling pathway and GNRHR pathway. The selective sweeps detected in this study contributed significantly to the phenotypic divergence observed between Attappady Black and Malabari goats in south India. [ABSTRACT FROM AUTHOR]
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- 2024
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31. Colonization profiles of gut microbiota in goat kids from neonatal to weaning period.
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Yuqin Wu, Dunying Hou, Siyuan Zhan, Linjie Wang, Jiaxue Cao, Jiazhong Guo, Li Li, Hongping Zhang, Lili Niu, and Tao Zhong
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COLONIZATION (Ecology) ,AMINO acid metabolism ,GUT microbiome ,ANIMAL weaning ,BACTERIAL colonies - Abstract
Understanding the colonization and change patterns of gut microbiota is pivotal for comprehending host health. As a newly cultured breed, the studies on the gut microbiota of Tianfu goats remain limited. This study aimed to address this gap by analyzing the microbial composition and colonization patterns of fecal samples collected from goat kids from birth to weaning. Fecal samples were collected on days 0, 7, 14, 21, 28, 35, 42, 49, 53, 55, 57, and 64, and the changes and colonization patterns of microorganisms were analyzed through high-throughput 16S rRNA sequencing. The results showed that the abundance of fecal microbiota in goat kids gradually increased over time, followed by a decrease after weaning and stabilization, with reduced individual differences. The colonization of fecal microorganisms mainly presented three different stages: days 0–14, days 21–49, and days 53–64. During the suckling period, the relative abundance of Proteobacteria (72.34%) was the highest, followed by Firmicutes (21.66%). From 21 days old, the microbiota in goat kids gradually to be diverse, with Lachnospiraceae and Ruminococcaceae being dominant. During post-weaning, Ruminococcaceae (30.98–33.34%) was becoming prominence which helpful for cellulose decomposition. LEfSe analyzed three important time points (d0 vs. d7, d7 vs. d14, d49 vs. d53, LDA score > 4 and p < 0.05), 53 microbial communities with stage differences were identified. Functional prediction using PICRUSt revealed that differential microbial communities are mainly related to carbohydrate and amino acid metabolism pathways. Overall, this study addresses the intricate relationship between ages, diets, and microbiota compositions in Tianfu goat kids, and also offering insights into microorganisms-host interactions. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Analysis of the zoonotic tick-borne encephalitis virus (TBEV) in raw milk and dairy products in mountain pastures of the Lombardy region, Italy.
- Author
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Scarazzato, Annalisa, Righi, Francesco, Sommariva, Marco Pietro, Bertoletti, Irene, Sala, Giovanni, Paterlini, Franco, Daminelli, Paolo, Finazzi, Guido, Losio, Marina-Nadia, and Pavoni, Enrico
- Subjects
TICK-borne encephalitis viruses ,TICK-borne encephalitis ,GOAT milk ,GOATS ,CENTRAL nervous system ,RAW milk - Abstract
Over the last few decades, tick-borne encephalitis (TBE) has become a growing public health problem in Europe. The tick-borne encephalitis virus (TBEV) is a zoonotic virus that affects the central nervous system (CNS). TBEV has been detected in 27 European countries, and the rise in TBE cases is mainly due to environmental and ecological factors, and factors that increase the risk of human exposure to infected ticks. The infection via the alimentary route is the second most common means of TBEV transmission to humans. Raw milk from infected goats, sheep, or cows has been identified as a source of human food-borne infections. This study aims to gather new information on the prevalence of tick-borne encephalitis virus (TBEV) in raw goat's and cow's milk and related raw products in the Lombard Alps (Italy). This is important due to the close proximity of Lombardy to the Triveneto region, where TBE is endemic, and southern Switzerland, where numerous TBEVpositive mammals have been found. Throughout 2023, a passive monitoring plan was implemented on samples delivered for TBEV analyses from the Alpine pastures. In total, 248 specimens including raw milk, raw milk cheese, and butter were tested. This is the first monitoring of food at risk of TBEV transmission in a non-endemic region with evidence of TBEV circulation. Despite testing a wide range of dairy products, no sample tested positive for RNA-TBEV by real-time RT-PCR. Preliminary results suggest that raw milk and raw dairy products do not pose a significant risk of TBEV transmission to humans in the territory of Lombardy. [ABSTRACT FROM AUTHOR]
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- 2024
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33. The Goat Cytotoxic T Lymphocyte-Associated Antigen-4 Gene: mRNA Expression and Association Analysis of Insertion/Deletion Variants with the Risk of Brucellosis.
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Wang, Congliang, Liu, Xiaoyu, Ren, Zhaofei, Du, Xiaomin, Li, Na, Song, Xiaoyue, Wu, Weiwei, Qu, Lei, Zhu, Haijing, and Hua, Jinlian
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GENE expression , *HAPLOTYPES , *BRUCELLOSIS , *BRUCELLA , *GENE frequency , *GOAT breeds - Abstract
The cytotoxic T lymphocyte-associated antigen-4 (CTLA4) gene, a member of the immunoglobulin superfamily, is crucial for maintaining immune homeostasis and preventing autoimmune diseases. Studies have shown that polymorphisms in the CTLA4 gene are linked to an increased risk of brucellosis in humans, but its association with brucellosis in goats remains unexplored. In this study, the tissue expression profile of CTLA4 in goats was investigated, and the correlation between InDel polymorphisms in the CTLA4 gene and susceptibility to brucellosis in goats was examined. The findings reveal the widespread expression of CTLA4 in goat tissues, particularly in the spleen and testes. The tested goat populations presented genotypes insertion/insertion (II), insertion/deletion (ID), and deletion/deletion (DD) at both the P1 and P2 loci, and an association analysis revealed significant differences in the distribution of genotypes and allele frequencies at the P1 and P2 loci of the CTLA4 gene between the Brucella goat case and the control groups (p < 0.05). Specifically, compared with the II genotype, the P1 and P2 loci were significantly associated with an elevated risk of brucellosis development in goats under both the codominant (ID/II) and dominant (ID + DD/II) models (P1, p = 0.042, p = 0.016; P2, p = 0.011, p = 0.014). Additionally, haplotype analysis indicated that haplotypes IP1DP2, DP1IP2, and DP1DP2 were significantly associated with an increased risk of brucellosis in goats compared to the reference haplotype IP1IP2 (p = 0.029, p = 0.012, p = 0.034). Importantly, the Lipopolysaccharide (LPS) stimulation of peripheral blood monocytes and/or macrophages from goats with the II, ID, and DD genotypes resulted in increased CTLA4 expression levels in the II genotype, leading to a robust LPS-induced inflammatory response. Through bioinformatic analysis, the observed effect of the InDel locus on Brucella pathogenesis risk in goats could be attributed to the differential binding of the transcription factors nuclear factor kappaB (NF-κB) and CCAAT/enhancer-binding protein α (C/EBPα). These findings offer potential insights for breeding strategies against brucellosis. [ABSTRACT FROM AUTHOR]
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- 2024
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34. Effects of nano-berberine and berberine loaded on green synthesized selenium nanoparticles on cryopreservation and in vitro fertilization of goat sperm.
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Piri, Mehrangiz, Mahdavi, Amir Hossein, Hajian, Mehdi, Nasr-Esfahani, Mohammad Hossein, Soltani, Leila, and Vash, Nima Tanhaei
- Abstract
After cryopreservation, reactive oxygen species (ROS) can damage sperm. Antioxidants are the primary defense against oxidative damage. Berberine is a bioactive alkaloid found in Berberis vulgaris, Curcuma longa, and Ergon grape, and is a potent antioxidant. Due to the negative effects of free radicals in oxidative stress processes, antioxidant chemicals are required to protect sperm. However, berberine has low bioavailability, making it less effective. Loading techniques on nanoparticles and nanotechnology can help overcome this limitation. Selenium nanoparticles were synthesized with barberry extract, and berberine was loaded on them. Berberine nanoparticles were then synthesized using anti-solvent precipitation with a syringe pump technique. The synthesis of nanoparticles was confirmed by EDX, UV-visible, FE-SEM, Zeta-Potential, and FTIR tests. In this experiment, we aim to investigate the impact of nano-berberine and berberine loaded on Se-NPs on goat sperm parameters after freeze-thawing. We assessed the generation of reactive oxygen species (ROS), in vitro fertility, and the subsequent embryo development of zygote with treated sperm after determining the optimal concentration of various chemicals on sperm parameters. The study found that all treatments had significant differences from the control group in terms of motility, viability, DNA and membrane integrity, ROS level, lipid peroxidation, in vitro fertility ability, and the capacity to develop inseminated oocytes (p < 0.05). The most significant outcomes were observed with berberine loaded on Se-NPs and the combination of selenium nanoparticles with berberine nanoparticles. [ABSTRACT FROM AUTHOR]
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- 2024
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35. The goat pan-genome reveals patterns of gene loss during domestication.
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Liu, Jiaxin, Shi, Yilong, Mo, Dongxin, Luo, Lingyun, Xu, Songsong, and Lv, Fenghua
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GOAT breeds , *GERMPLASM conservation , *GENETIC variation , *PAN-genome , *GOATS - Abstract
Background: Unveiling genetic diversity features and understanding the genetic mechanisms of diverse goat phenotypes are pivotal in facilitating the preservation and utilization of these genetic resources. However, the total genetic diversity within a species can't be captured by the reference genome of a single individual. The pan-genome is a collection of all the DNA sequences that occur in a species, and it is expected to capture the total genomic diversity of the specific species. Results: We constructed a goat pan-genome using map-to-pan assemble based on 813 individuals, including 723 domestic goats and 90 samples from their wild relatives, which presented a broad regional and global representation. In total, 146 Mb sequences and 974 genes were identified as absent from the reference genome (ARS1.2; GCF_001704415.2). We identified 3,190 novel single nucleotide polymorphisms (SNPs) using the pan-genome analysis. These novel SNPs could properly reveal the population structure of domestic goats and their wild relatives. Presence/absence variation (PAV) analysis revealed gene loss and intense negative selection during domestication and improvement. Conclusions: Our research highlights the importance of the goat pan-genome in capturing the missing genetic variations. It reveals the changes in genomic architecture during goat domestication and improvement, such as gene loss. This improves our understanding of the evolutionary and breeding history of goats. [ABSTRACT FROM AUTHOR]
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- 2024
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36. Environmental contamination of arsenic: pathway analysis through water-soil-feed-livestock in Nadia District (India) and potential human health risk.
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Yadav, Sushil Kumar, Dutta, Tapas Kumar, Chatterjee, Anupam, Dutta, Sneha, Mohammad, Asif, and Das, Arun Kumar
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FEED analysis ,GOATS ,GOAT milk ,WELLS ,CONCENTRATE feeds ,PLANT cell walls ,ERECTOR spinae muscles - Abstract
This study investigated arsenic (As) concentrations in diverse environmental components and their potential impact on the health risks faced by residents of the arsenic (As)-contaminated Nadia district in West Bengal, India. A random selection of 182 cattle and 255 goats from 40 livestock farmers in the district revealed that both animals and humans were naturally exposed to elevated arsenic levels through contaminated drinking water, foods, grasses, concentrate feeds, various fodder tree leaves, and other food/feed resources. The mean As concentration in roughages (483.18 µg/kg DM) was significantly higher (p < 0.001) than in tree leaves (391.53 µg/kg DM), and concentrate feed/ingredients (186.66 µg/kg DM). Pond water exhibited higher arsenic levels (106.11 µg/L) compared to shallow tube well water (47.96 µg/L) and deep tube well water/tap water (10.64 µg/L and 10.04 µg/L, respectively). The mean arsenic concentration in soils DM of fodder fields, crop fields, and grassland was 10.25, 10.58, and 10.20 mg/kg, respectively. It was observed that protein-rich feeds had lower levels of arsenic accumulation (p < 0.048), while fiber-rich feeds containing more cellulose, hemicellulose, and lignin had higher arsenic levels (p < 0.017). Goats consumed 73.46% more arsenic per kg body weight compared to dairy cows. Although chronic and sub-chronic arsenic exposure in the district did not typically manifest symptoms or visible signs in ruminant animals, concentrations in the hair and feces of both cattle and goats exceeded normal values. Cattle feces had significantly higher arsenic (410.43 µg/kg DM) levels (p < 0.001) than goat feces (227.00 µg/kg DM), and arsenic concentration in cattle hair (1917.74 µg/kg DM) was also significantly greater (p < 0.001) than goat hair (1435.74 µg/kg DM). Arsenic levels in milk samples from both species were below 10 µg/kg. Liver (356.02 µg/kg DM) and kidney (317.22 µg/kg DM) contained significantly higher (p < 0.001) levels of arsenic compared to muscle (204.23 µg/kg DM), and bone (161.98 µg/kg DM) in local meat-type adult male goats. The skin accumulated the highest amount of arsenic (576.24 µg/kg DM) among the non-edible parts of the goat carcass. The cumulative cancer risk value for adults was 4.96 × 10
−3 , exceeding the threshold value (1 × 10−6 ). This suggests a significant risk of cancer development for the population in arsenic-affected areas. Non-cancer risks (hazard indexes) were estimated at 11.01 for adults. Our observations revealed that the highest bioaccumulation of arsenic occurred in the hair of cows, and goats in the examined localities. The biotransformation factor (BTF) for hair was much higher compared to other excreted samples from both species. The calculated BTF followed the order: hair > feces > milk for cows and goats. Livestock farmers in Nadia district are advised to carefully select feed resources, prioritizing those high in crude protein and low in neutral detergent fiber, and they should provide drinking water from deep aquifers to ensure the safety of milk and meat for human consumption. [ABSTRACT FROM AUTHOR]- Published
- 2024
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37. The Molecular Detection and Therapeutic Management of Pathogenic Theileria luwenshuni Infection in a Goat: A Case Report.
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Arif, Syed Abdul, Lahkar, Deepa, Gogoi, Sophia Makdoh, Changkija, Bendangla, Kakati, Parikshit, Buragohain, Lukumoni, Pathak, Mamta, and Das, Tinku
- Abstract
A 2-year-old female Assam Hill goat was presented with a clinical history of anorexia, fever, mild anemia, rough body coat, dehydration, tachycardia, dyspnea and swelling of palpable lymph nodes. Hematology revealed low hemoglobin, packed cell volume, red blood cell and thrombocyte count. Biochemical analysis showed increased serum concentration of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and urea in comparison to the normal reference range. Microscopic examination showed intra-erythrocytic forms of Theileria species. Molecular and phylogenetic analysis of partial 18S rRNA gene sequence confirmed Theileria luwenshuni infection. The goat was treated with buparvaquone and oxytetracycline and recovered uneventfully. A three-month follow-up showed no recurrence. This study reveals the presence of T. luwenshuni in Assam, India and it should be considered in differential diagnosis and as one of the important pathogens of clinically sick goats. The present case report provides a rational approach to diagnosis and treatment for a goat infected with pathogenic T. luwenshuni in Assam, India. To our knowledge, the present communication describes about the first successful therapeutic management of pathogenic T. luwenshuni infection in a goat supported with molecular evidence from Assam, a north-eastern state of India. [ABSTRACT FROM AUTHOR]
- Published
- 2024
38. Frequency of Shiga Toxin Associated Genes in Escherichia coli Isolated from Salivary Abomasum Disease in Kid Goats.
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Chegeni, Saba Almasi, Esmaeili, Hossein, and Lotfalizadeh, Peyman
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ESCHERICHIA coli , *ANIMAL herds , *GOAT diseases , *ACUTE diseases , *TOXINS - Abstract
Background: Salivary abomasum disease (SAD) is a devastating disease causing significant mortality in Iranian goat and sheep herds. Understanding the causative agents is essential for developing effective preventive measures. This study investigated the potential role of Shiga toxin-producing Escherichia coli (STEC) in SAD pathogenesis. Methods: We isolated E. coli from kid goats aged 3-30 days experiencing a sudden, acute illness characterized by gait imbalance, and death within 48 hours during the kidding season. Following isolation, we employed multiplex PCR to identify the presence of Shiga toxin genes (Stx1 and Stx2) associated with virulence in STEC strains. Results: E. coli was isolated from 30 (75%) out of 40 animals. Notably, 7 (23.3%) isolates harbored the Stx2 gene, while only one isolate (3.3%) possessed the Stx1 gene. Conclusion: These findings suggest a potential role for STEC, particularly strains carrying the Stx2 gene, in the development of SAD and multiple abomasal hemorrhages, in kid goats. The presence of Shiga toxin genes in a significant proportion of E. coli isolates highlights the importance of further research to elucidate their contribution to SAD pathogenesis and inform the development of targeted interventions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
39. Milk Production, Milk Composition, Milk Fatty Acid Profile and Nutrient Digestibility of Mehsana Goats Fed Sunflower (Helianthus annus) Oil.
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Raut, A. S., Patil, S. S., Pawar, M. M., Patel, M. P., Gupta, J. P., and Modi, C. P.
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SHORT-chain fatty acids , *UNSATURATED fatty acids , *SUNFLOWER seed oil , *GOAT milk , *MILKFAT - Abstract
A study was conducted during the month of December, 2020 to January, 2021 at Livestock Research Station, Kamdhenu University, Sardarkrushinagar, Gujarat (385506), India to evaluate the effect of dietary sunflower oil supplementation on milk yield, milk fatty acid profile and nutrient digestibility of Mehsana goats. Eighteen lactating Mehsana goats were randomly divided into three treatment groups, viz. Basal diet (control), Basal diet+25 g animal-1 day-1 of sunflower oil and Basal diet+50 g animal-1 day-1 of sunflower oil for a period of 60 days. No significant differences (p>0.05) were found in milk yield in terms of kg d-1, 4% fat corrected milk and energy corrected milk among the treatment groups. The milk constituents and their yields were not affected due to dietary inclusion of sunflower oil. Sunflower oil feeding at the dose of 50 g d-1 increased (p<0.05) concentration of milk short chain fatty acids and polyunsaturated fatty acids in the milk of goats. The intake and digestibility of nitrogen free extract was increased in sunflower oil supplemented groups. The intake and digestibility of dry matter, organic matter, crude protein, ether extract and crude fibre were similar (p>0.05) among the treatment groups. it may be concluded that supplementation of sunflower oil at the dose rate of 25 and 50 g d-1 did not affect feed intake, milk yield, milk constituents and nutrient digestibility in lactating Mehsana goats. However, dietary 50 g d-1 of sunflower oil significantly increased concentration of milk short chain fatty acids and polyunsaturated fatty acids in goats. [ABSTRACT FROM AUTHOR]
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- 2024
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40. Evaluation of Placentome Morphology in the Last Two Periods of Pregnancy in Hair Goats (Capra aegagrus hircus).
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İşbilir, Fatma, Kandil, Banu, İşbilir, İhsan, Koca, Davut, and Güzel, Barış Can
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GOATS , *FETAL development , *MORPHOMETRICS , *NEOVASCULARIZATION , *MORPHOLOGY - Abstract
This study was carried out on pregnant uteruses obtained from healthy Hair goats (Capra aegagrus hircus). A total of thirteen pregnant uteruses, six second and seven third trimesters, were used. Morphometrically, placentome numbers, lengths, widths and depths were measured. Randomly selected placentomes from the second and third trimesters were stained with the triple staining method. Caruncular and cotyledonary areas, capillary numbers and areas were measured using Qupath v0.5.0 software. The Minitab (version 21.4.1) was used for statistical analysis. While no statistical difference was observed in the number of placentomes between trimesters (p > 0.05), placentome length, width and depth were higher in the third trimester compared to the second trimester (p < 0.001). No difference was observed in the number of caruncular and cotyledonary capillaries in the second trimester. In the second trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.05). Both caruncular and cotyledonary capillary area parameters were higher in the third trimester than in the second trimester (p < 0.001). In the third trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.001). The number of caruncular capillaries and cotyledonary capillaries was statistically significantly higher in the third trimester compared to the second trimester. In addition, the number of cotyledonary capillaries was higher than that of caruncular capillaries in the third trimester (p < 0.001). A positive and significant correlation was found between the day of pregnancy and the number of placentomes in the second trimester (p < 0.05). No correlation was observed between the day of pregnancy and the number of placentomes in the third trimester. Vascular area density showed a faster development in foetal tissue than in maternal tissue. Placentome size and angiogenesis increased with the progression of pregnancy. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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41. Low Testosterone Concentration Improves Colonisation and Viability in the Co‐Cultured Goat Spermatogonial Stem Cell With Sertoli Cells.
- Author
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Salimi, Hossein, Rahimi Feyli, Peyman, Yari, Kheirollah, Wong, Alexei, and Moghaddam, Ali Asghar
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- *
SERTOLI cells , *GERM cells , *GOAT milk , *TRANSGENIC animals , *STEM cells - Abstract
Spermatogonial stem cells (SSCs) maintain spermatogenesis through self‐renewal and differentiation. The proliferation of SSCs in culture systems can provide a valuable source of germ cells. Several studies have investigated new reproductive technologies, including the production of transgenic animals and recombinant proteins secreted from milk in goats. While studies in other species exist, research on goat SSC culture remains limited. We investigated the impact of different testosterone concentrations on the survival and colonisation of cocultured goat SSCs with Sertoli cells. Cells were isolated from immature goats using two‐step enzymatic digestion and enriched by differential exclusion method. DMEM/F12 culture medium containing 1% antibiotic and 5% FBS, supplemented with GDNF (20 ng/mL), EGF, bFGF and LIF (10 ng/mL), was used with different testosterone concentrations (0, 60, 120 and 240 μg/mL) and cultured for 10 days. SC subpopulations were confirmed using PGP9.5 immunocytochemistry, and the expression of germ cell markers (ID‐4, UCHL‐1, THY‐1, β1‐integrin, BCL6B, VASA, PLZF and OCT‐4) was evaluated through RT‐PCR. Alkaline phosphatase activity provided additional SSC presence. The survival rate of SSCs after isolation and the number and area of colonies on Days 4, 7 and 10 were measured using an inverted microscope. The presence of PGP 9.5 antigens and germ cell markers (ID‐4, UCHL‐1, THY‐1, β1‐integrin, BCL6B, VASA, PLZF and OCT‐4) was confirmed by immunocytochemistry and RT‐PCR, respectively. According to the results, the group with 60 μg/mL testosterone had the highest number and area of colonies. The number of colonies in the 60 μg/mL testosterone group was significantly higher than the control group (p < 0.05), but no significant difference was observed compared to other groups (p ≥ 0.05). This study suggests that a low testosterone concentration (60 μg/mL) is optimal for goat SSC colonisation and viability in coculture with Sertoli cells, potentially leading to advancements in goat reproductive technologies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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42. Supplementation of Plant‐Based Freezing Extender With Cysteamine Preserves Quality Parameters and Fertility Potential of Buck Sperm During Cryopreservation Process.
- Author
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Mokhtari, Mahdi, Khodaei‐Motlagh, Mahdi, Yahyaei, Mohammad, and Masoudi, Reza
- Subjects
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SEMEN analysis , *FERTILITY , *FROZEN semen , *SPERMATOZOA , *SEMEN , *MORPHOLOGY - Abstract
Sperm cryopreservation in small ruminant is an efficient strategy to distribute spermatozoa for reproductive programmes, but this process reduces the fertility potential of frozen–thawed spermatozoa. The aim of the current research was to evaluate the impact of different concentrations of cysteamine (CYS) in soybean lecithin (SL)‐based medium on postthawed buck semen quality and fertility potential. Semen samples were collected from five bucks, twice a week, then diluted in the SL‐based extender containing different concentrations of CYS as follows: extender containing 0 mM (control, C0), 1 mM (C1), 2 mM (C2), 4 mM (C4) and 8 mM (C8) CYS. Motility characteristics, membrane integrity, abnormal morphology, mitochondrial activity, acrosome integrity, viability, apoptotic‐like changes, lipid peroxidation, DNA fragmentation, ROS concentration, pregnancy rate and kidding rate were evaluated after freeze–thaw process. In results, C1 resulted in greater (p ≤ 0.05) total motility, progressive motility, average path velocity, membrane integrity, mitochondrial activity, acrosome integrity, viability, pregnancy rate and kidding rate compared to the other groups. Furthermore, supplementation of freezing medium with 1 mM of CYS presented lower (p ≤ 0.05) apoptotic‐like changes, lipid peroxidation, DNA fragmentation and ROS concentration compared to the other groups. On the other hand, C8 presented the least (p ≤ 0.05) total motility, progressive motility, average path velocity, membrane integrity, mitochondrial activity, acrosome integrity and viability as well as the highest (p ≤ 0.05) apoptotic‐like changes, lipid peroxidation, DNA fragmentation and ROS concentration compared to the other groups. Therefore, supplementation of freezing medium with 1 mM CYS could be a helpful strategy to protect buck's spermatozoa quality and fertility potential during cryopreservation process. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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43. Multiomic meta‐analysis suggests a correlation between steroid hormone‐related genes and litter size in goats.
- Author
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Liu, Cheng‐Li, Mou, Hui‐Long, Na, Ri‐Su, Wang, Xiao, Hu, Peng‐Fei, Ceccobelli, Simone, Huang, Yong‐Fu, and E, Guang‐Xin
- Subjects
- *
STEROID synthesis , *GENE expression , *GOAT breeds , *GENE regulatory networks , *PEARSON correlation (Statistics) , *OVARIAN follicle - Abstract
Litter size is a key indicator of production performance in livestock. However, its genetic basis in goats remains poorly understood. In this work, a genome‐wide selection sweep analysis (GWSA) on 100 published goat genomes with different litter rates was performed for the first time to identify candidate genes related to kidding rate. This analysis was combined with the public RNA‐sequencing data of ovary tissues (follicular phase) from high‐ and low‐yielding goats. A total of 2278 genes were identified by GWSA. Most of these genes were enriched in signaling pathways related to ovarian follicle development and hormone secretion. Moreover, 208 differentially expressed genes between groups were obtained from the ovaries of goats with different litter sizes. These genes were substantially enriched in the cholesterol and steroid synthesis signaling pathways. Meanwhile, the weighted gene co‐expression network was used to perform modular analysis of differentially expressed genes. The results showed that seven modules were reconstructed, of which one module showed a very strong correlation with litter size (r = −0.51 and p‐value <0.001). There were 51 genes in this module, and 39 hub genes were screened by Pearson's correlation coefficient between core genes > 0.4, correlation coefficient between module members > 0.80 and intra‐module connectivity ≥5. Finally, based on the results of GWSA and hub gene Venn analysis, seven key genes (ACSS2, HECW2, KDR, LHCGR, NAMPT, PTGFR and TFPI) were found to be associated with steroid synthesis and follicle growth development. This work contributes to understanding of the genetic basis of goat litter size and provides theoretical support for goat molecular breeding. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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44. ADAR1 Promotes Myogenic Proliferation and Differentiation of Goat Skeletal Muscle Satellite Cells.
- Author
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Zhao, Zihao, Xiao, Miao, Xu, Xiaoli, Song, Meijun, Dai, Dinghui, Zhan, Siyuan, Cao, Jiaxue, Guo, Jiazhong, Zhong, Tao, Wang, Linjie, Li, Li, and Zhang, Hongping
- Subjects
- *
ADENOSINE deaminase , *SATELLITE cells , *MUSCLE growth , *ANIMAL culture , *DOMESTIC animals , *PSOAS muscles , *SKELETAL muscle - Abstract
As one of the most important economic traits for domestic animal husbandry, skeletal muscle is regulated by an intricate molecular network. Adenosine deaminase acting on RNA (ADAR1) involves various physiological processes and diseases, such as innate immunity and the development of lung adenocarcinoma, breast cancer, gastric cancer, etc. However, its role in skeletal muscle growth requires further clarification. Here, we explored the functions of ADAR1 in the myogenic process of goat skeletal muscle satellite cells (MuSCs). The ADAR1 transcripts were noticeably enriched in goat visceral tissues compared to skeletal muscle. Additionally, its levels in slow oxidative muscles like the psoas major and minor muscles were higher than in the fast oxidative glycolytic and fast glycolytic muscles. Among the two common isoforms from ADAR1, p110 is more abundant than p150. Moreover, overexpressing ADAR1 enhanced the proliferation and myogenic differentiation of MuSCs. The mRNA-seq performed on MuSCs' knockdown of ADAR1 obtained 146 differentially expressed genes (DEGs), 87 upregulated and 59 downregulated. These DEGs were concentrated in muscle development and process pathways, such as the MAPK and cAMP signaling pathways. Furthermore, many DEGs as the key nodes defined by protein–protein interaction networks (PPI), including STAT3, MYH3/8, TGFβ2, and ACTN4, were closely related to the myogenic process. Finally, RNA immunoprecipitation combined with qPCR (RIP-qPCR) showed that ADAR1 binds to PAX7 and MyoD mRNA. This study indicates that ADAR1 promotes the myogenic development of goat MuSCs, which provides a useful scientific reference for further exploring the ADAR1-related regulatory networks underlying mammal skeletal muscle growth. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
45. Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes.
- Author
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Ferrer‐Roda, Mònica, Catalán, Jaime, Izquierdo, Dolors, Oliveira, Maria Emilia Franco, and Paramio, Maria‐Teresa
- Subjects
- *
LIFE sciences , *ACROSOME reaction , *SPRING , *INTRACELLULAR calcium , *AUTUMN , *SPERMATOZOA , *FROZEN semen , *SEMEN - Abstract
Goat production is affected by reproductive seasonality. In vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM‐199 with hormones, EGF and cysteamine; fertilized and cultured in BO‐IVF and BO‐IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3 h after culture in capacitating (BO‐IVF, CAP) and non‐capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12.0% vs. 2.1%, respectively; p <.05). After CAP, acrosome reaction and intracellular calcium were higher (p <.05) in spring than autumn sperm. No differences were found in other sperm parameters. In conclusion, seasonal variations in the IVEP of prepubertal goats could be linked to differences in sperm ability to undergo in vitro capacitation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
46. Parasite diversity among domestic goats of tropical monsoon climatic zone in India.
- Author
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Dittrich, Sonja Pia, Adithya, Sasi, Ajith, Y., Athira, N., Athira, K. S., Safeer, M. Saifudeen, Preena, P., Aishwarya, A., Athira, K., Nisha, A. R., Devi, Gopinath, Mäder, Mia, Beena, V., and Jacob, Siju Susan
- Abstract
Among different climatic zones in India, the tropical monsoon region comprises a diverse ecosystem characterized by the endemic nature of several parasites including certain emerging and re-emerging vector-borne pathogens of humans, whereas a systematic investigation of the occurrence of different parasites among domestic goats in this area is not yet explored. The goal of the present study is to explore the parasite diversity focusing on molecular identification of vector-borne hemoparasites and its health impacts on domestic goats reared in the tropical monsoon climate zone of Kerala, India. Among 227 goats presented to the Teaching Veterinary Clinical Complex (TVCC) in the monsoon months of 2023, thirty animals were recruited for the study. The animals were screened for the presence of different hemoparasites (Anaplasma spp., Theileria spp., and Babesia spp.), ectoparasites (ticks, lice, and fleas), and gastrointestinal (GI) parasites (hookworms, threadworms, tapeworms, whipworms, and coccidia). The isolated hemoparasites were further characterized by sequencing and phylogenetic analysis. The correlation studies to elucidate the association between the occurrence of different parasites and clinical manifestations (hyperthermia, pallor of mucous membrane, circulatory failure, respiratory signs, neurological instability, and GI signs), blood picture (anemia, leukopenia, thrombocytopenia), demographics (sex and age), and treatment history (hemoparasitic therapy, ectoparasiticidal application, and prophylactic deworming) were conducted. The co-infection status of these parasites was also evaluated. A substantial portion of the goats in the study group was found to be affected by vector-borne hemoparasitic diseases and their arthropod vectors or GI parasites or both. This can be attributed to the constantly warm and humid climate of the region, which is favorable for the survival and growth of different life cycle stages of these parasites and vectors. A strategic parasitic disease surveillance-cum-control program is the need of the hour for ensuring climate resilience and profitable goat farming in the region. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. Molecular characterization of Peste des petits ruminants virus (PPRV) in sheep and goats and risk factors associated with it in selected districts of Khyber Pakhtunkhwa-Pakistan.
- Author
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Awaz, Saira, Maqsood, Iram, Rahman, Hanif Ur, Ali, Muhammad Ijaz, Khan, Baitullah, Muhammad, Gul, Shah, Imtiaz Ali, Azam, Asima, Hidayat, Ayesha, and Nizam, Almas Faryal
- Abstract
Background: Peste des Petits Ruminants (PPR) is an economically significant transboundary viral disease of sheep and goats caused by the PPRV virus, affecting annual losses of 1.45–2.10 billion US dollars globally. We designed the current study to evaluate the positive cases, molecular characterization, phylogenetic analysis, and risk factors correlated with the disease in various districts of Khyber Pakhtunkhwa, Pakistan, with the aim of contributing to these strategies. Methods and results: A total of 384 samples from three selected districts, i.e., Peshawar, Charsadda and Chitral (n = 128 each), were collected, and the virus was investigated by using the sandwich ELISA, while the N gene of the virus was used as a target for molecular detection via RT-PCR. The confirmed samples were then sequenced, and phylogenetic analysis was performed. According to our findings, the highest positive cases was found in district Peshawar (50.87%), followed by Charsadda and Chitral (24.56%), respectively, while risk factor analysis showed that certain categories, such as species, sex, and age less than two years, have higher risk (P < 0.05) in contrast to their respective categories. Furthermore, sequencing and phylogenetic analysis of representative samples showed that the PPRV strains in the current study clustered in lineage IV, which is circulating in the small ruminant population of Asia, the Middle East, and African countries. Comparative residue analysis highlighted the mutation by representing 242 variable sites out of 371 locations. Conclusions: PPRV has foremost importance in Pakistan because the virus was detected in a considerable number of samples, and most of which were sourced from subsidiary areas where veterinary services are not prioritized. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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48. Unconventional Ingredients from the Industrial Oilseed By-Products in Dairy Goat Feeding: Effects on the Nutritional Quality of Milk and on Human Health.
- Author
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Tristan Asensi, Marta, Pagliai, Giuditta, Napoletano, Antonia, Lotti, Sofia, Dinu, Monica, Mannelli, Federica, Invernizzi, Guido, Sofi, Francesco, Colombini, Barbara, and Buccioni, Arianna
- Abstract
Oilseed by-products (Cynara cardunculus and Camelina sativa) (CCCS) are rich in bioactive compounds. This study aimed to evaluate the health effects of consuming yogurt made from goat milk fed with CCCS industrial residues in adults. In this randomized, crossover clinical trial, 20 healthy adults (14F; 37.7 ± 14.2 years) consumed either yogurt made from goat milk fed with CCCS or regular goat yogurt (C) daily for 1 month in each phase. Anthropometric parameters and blood samples were collected at the beginning and end of each phase. CCCS yogurt consumption resulted in a significant fat mass reduction (−1.8% and −1.1 kg) and fat-free mass increase (+1.5% and +0.9 kg). Regarding blood parameters, a non-significant decrease in triglycerides, total cholesterol, and LDL cholesterol was observed, particularly after the CCCS intervention. Moreover, creatinine levels exhibited an opposite trend (p = 0.023) after CCCS, decreasing in subjects aged ≤30 years (−0.03 mg/dL) and increasing in older subjects (+0.05 mg/dL). Regarding inflammatory parameters, a non-significant trend in increased IL-1ra levels was observed especially after CCCS yogurt consumption compared to the C yogurt (+56.9 vs. +19.1 pg/mL, respectively). The use of unconventional feed derived from oilseed by-products for dairy goat feeding may have potential possible beneficial effects on human health. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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49. Recombinant 3AB3 nonstructural protein-based indirect ELISA for detection of foot-and-mouth disease virus infection-elicited antibodies in goat.
- Author
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Rout, M., Pandey, L. K., Prusty, B. R., Samanta, R., Mohapatra, J. K., and Singh, R. P.
- Abstract
Foot-and-mouth disease (FMD) is one of the most important animal diseases of economic significance globally. It is a highly infectious and contagious disease of cloven-hoofed animals including sheep and goat. For sero-diagnosis of FMD, recombinant antigen-based assays are considered as alternatives to conventional approaches such as the liquid phase blocking ELISA (LPBE). The early interventions towards control measures cannot be implemented unless the disease gets promptly diagnosed. It is relatively difficult to clinically diagnose FMD in goat due to the usual milder form or unapparent nature of symptoms. Under such situations where clinical samples are not available, demonstration of infection-specific FMD virus (FMDV) antibodies in serum sample may help identifying the animals exposed to the virus in retrospect. Antibody to 3AB nonstructural protein (NSP) has been considered to be the most reliable indicator for FMD diagnosis. The current study extended the earlier designed recombinant 3AB3 protein-based indirect ELISA originally validated on bovine serum samples to testing serum samples of goat. The performance of the indirect ELISA was validated using internationally accepted PrioCHECK
® FMDV NS kit. The overall diagnostic sensitivity (DSn) of the indirect ELISA was estimated to be 95.52% (619/648), while the diagnostic specificity (DSp) on naïve and vaccinated animals varied at 98.06% (557/568) and 94.15% (435/462), respectively. In India, where FMD is prevalent and the goat population is so high, this 'in-house' optimized assay can be considered to be an adjunct in sero-epidemiological investigation of FMD in goat. [ABSTRACT FROM AUTHOR]- Published
- 2024
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50. A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
- Author
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Ortega J, Agulló-Ros I, Roy A, Moreno I, Gómez-Buendía A, Romero B, Ferreras-Colino E, de Juan L, Domínguez M, Domínguez L, Risalde M.A, and Bezos J
- Subjects
Tuberculosis ,goat ,immunisation ,P22 protein immunocomplex ,diagnosis ,lesions ,Veterinary medicine ,SF600-1100 - Abstract
Caprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC), contributing to tuberculosis (TB) transmission among animals and humans. The P22 protein immunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacity and is used for TB diagnosis. This study assessed the immunogenicity and protective efficacy of P22PI in two groups of goats: 24 naïve goats (12 immunised, 12 controls) from a TB-free herd, and 24 infected goats (12 immunised, 12 controls), referred to as pre-infected animals, from a M. bovis-infected herd. Both were exposed for 5 months to M. bovis-naturally infected goats. Reactors to single and comparative intradermal tuberculin (SIT and SCIT, respectively) tests and interferon-gamma release assay (IGRA) significantly increased (p
- Published
- 2024
- Full Text
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