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Objective To establish a rapid method for the analysis of bucinnazine in blood by UPLC-MS/MS and to apply the method to the practical case. Methods After the internal standard was added to blood, the protein was precipitated with 900 μL mixed solution （Vacetonitrile∶Vwater=8∶2）. After vortex and centrifugation, the protein was measured through 0.22 μm filter membrane. The separation was performed on C18 chromatography column, with acetonitrile and 5 mmol/L ammonium acetate containing 0.1% formic acid aqueous as mobile phase gradient elution at the flow rate of 0.4 mL/min. Multiple reaction monitoring scan was performed in electrospray positive ion mode, quantitative measurement was performed by internal standard method, and methodological verification was carried out. Results The linear relationship of bucinnazine in blood was good in the range of 0.5-200 μg/L, the correlation coefficient （r） was 0.999 7, the limit of detection was 0.1 μg/L, the limit of quantitation was 0.5 μg/L, and the recovery was 78.3%-83.8% at 1, 10 and 100 μg/L mass concentration levels. The matrix effect was 69.4%-73.8%, the intra-day precision was 1.9%-2.8%, and the inter-day precision was 2.8%-3.2%, the accuracy was 3.1%-3.5%. The stability test results of 1 and 100 μg/L mass concentrations at -25 ℃ showed that the accuracy （bias） of 10 d was less than 4.5%. Conclusion This method has the advantages of simple pre-treatment process, fast sample processing speed, high sensitivity of instrument analysis, good stability of content determination and reliable identification results, and can meet the needs of case identification.

Hypertriglyceridemic waist (HW), hypertriglyceridemic waist-to-height ratio (HWHtR), and waist-to-hip ratio (WHR) have been shown to be indicators of cardiometabolic risk factors. However, it is not clear which indicator is more suitable for children and adolescents. We aimed to investigate the relationship between HW, HWHtR, WHR, and cardiovascular risk factors clustering to determine the best screening tools for cardiometabolic risk in children and adolescents. This was a national cross-sectional study. Anthropometric and biochemical variables were assessed in approximately 70,000 participants aged 6–18 years from seven provinces in China. Demographics, physical activity, dietary intake, and family history of chronic diseases were obtained through questionnaires. ANOVA, χ2 and logistic regression analysis was conducted. A significant sex difference was observed for HWHtR and WHR, but not for HW phenotype. The risk of cardiometabolic health risk factor clustering with HW phenotype or the HWHtR phenotype was significantly higher than that with the non-HW or non-HWHtR phenotypes among children and adolescents (HW: OR = 12.22, 95% CI: 9.54-15.67; HWHtR: OR = 9.70, 95% CI: 6.93-13.58). Compared with the HW and HWHtR phenotypes, the association between risk of cardiometabolic health risk factors (CHRF) clustering and high WHR was much weaker and not significant (WHR: OR = 1.14, 95% CI: 0.97-1.34). Compared with HWHtR and WHR, the HW phenotype is a more convenient indicator with higher applicability to screen children and adolescents for cardiovascular risk factors. [ABSTRACT FROM AUTHOR]

Objective To investigate the computed tomography (CT) and clinical characteristics of patients with extranodal natural killer/T-cell lymphoma, nasal type ( ENKTL) as well as the reason of misdiagnosis. Method The CT and clinical findings of 25 patients with nasal type extranodal NK/T-cell lymphoma were retrospectively reviewed, and the reasons of misdiagnosis were analyzed to avoid it. Results Of the 25 patients, 9 of 15 patients who had a sinus CT were misdiagnosed for sinusitis before admitted to our hospital. According to the extent of tumors, 16 patients showed localized type, the localized lesions were found in the anterior nasal cavity in 14 cases and in posterior part in 2 cases. On the basis of its properties, CT showed that soft tissue density tumor filled the nasal cavity, and spread along the nasal mucosa, enhanced CT showed mild to moderate heterogeneous enhancement. For localized types, bone mass showed no obvious change; for diffuse type, bone mass destruction can be showed along the middle of nasal cavity. For localized type patients, the lesions showed homogeneous density lesion in 5 cases and inhomogeneous density in 9 cases, only 2 cases showed bone change. For diffuse type patients, the lesions showed homogeneous density lesion in 5 cases and heterogeneous enhancement after enhanced CT in 4 cases. 4 cases extended to septum and orbit and hard palate and pterygopalatine fossa. Conclusion Computed tomography (CT) and clinical findings have specific manifestation which suggest the diagnosis in nasal type extranodal NK/T-cell lymphoma patients. But for localized type cases and early stages patients, no obvious specificity can be showed by imaging findings of CT and clinical symptom. Only by CT combined with clinical symptoms we can diagnose it. [ABSTRACT FROM AUTHOR]

Objective To investigate the expression and significance of TLR-2 and HO-1 in recurrent nasal polyps, and to discuss the relationship between the expression and the recurrence of nasal polyps. Methods The immune protein imprinting technology was used to detect the protein expression of TLR-2 and HO-1 in 20 cases of recurrent nasal polyps, 20 cases of nasal polyps and 20 cases of normal mucosal tissue in control group, and the relationship between the two proteins were analyzed. Results Expression levels of TLR-2 and HO-1 in the tissues of the recurrent nasal polyps had statistically significant difference with nasal polyps group (P < 0.05). Expression levels of TLR-2 and HO-1 in the tissues of the nasal polyps had statistically significant difference with normal nasal mucosa tissues (P< 0.05). The expression of TLR-2 and HO-1 had significant correlation in each group (P < 0.05). Conclusion TLR-2 and HO-1 plays an important role in the recurrence mechanism of nasal polyps, and both of them may be as objective indicators for postoperative follow-up of patients with recurrent nasal polyps and judging the recurrent trend. [ABSTRACT FROM AUTHOR]