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12. Chapter 10 - Kale

Author

Pellicer, José Antonio, Fortea, María Isabel, Rodríguez-López, María Isabel, Hernández-Sánchez, Pilar, López-Miranda, Santiago, Gabaldón, José Antonio, and Núñez-Delicado, Estrella

Published
2020
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14. List of Contributors

Author

Albuquerque, Tânia Gonçalves, Amara, Sana Ben, Antony, Usha, Apak, Reşat, Balzano, Michele, Barboza, Henriqueta Talita Guimarães, Bartolucci, Edoardo, Başkan, Kevser Sözgen, Bernhardt, Dana Cecilia, Bhatt, Indra D., Bhattacharjee, Chiranjib, Bohn, Torsten, Boonruamkaew, Phetcharat, Bouayed, Jaouad, Carbonell-Barrachina, Ángel A., Caruso, Gianluca, Carvalho, Ana Maria, Chaurasia, Savita, Chonpathompikunlert, Pennapa, Chouksey, Anita, Coelho, Caroline Corrêa de Souza, Costa, Helena S., Das, Ranjana, Debnath, Samir C., Díaz, María Teresa Blanco, Domingo, Cinthia Santo, Ersoy, Şeyda Karaman, Evrendilek, Gulsun Akdemir, Falco, Tiziana, Fattouch, Sami, Fernández-López, Juana, Ferreira, Isabel C.F.R., Fibiani, Marta, Fissore, Eliana Noemi, Font, Rafael, Fortea, María Isabel, Frega, Natale G., Freitas-Silva, Otniel, Gabaldón, José Antonio, García-Alonso, Francisco-Javier, García-Valverde, Verónica, Gerschenson, Lía Noemi, Ghazouani, Tesnime, Golubkina, Nadezhda, Gómez, Pedro, Gonzalez, Julia, González-Aguilar, Gustavo A., González-Barrio, Rocío, Guillén-Bejarano, Rafael, Hamdi, Amel, Harper, Niamh, Hdider, Chafik, Hepsiba, Alice, Hernández-García, Francisca, Hernández-Sánchez, Pilar, Ilahy, Riadh, Jaiswal, Amit K., Jaiswal, Swarna, Jaramillo-Carmona, Sara, Jayaprakasha, G.K., Jiménez-Araujo, Ana, Jiménez-Monreal, Antonia M., Kalita, Dipankar, Lei, Yun, Leporini, Mariarosaria, Li, Chunyang, Lo Scalzo, Roberto, Loizzo, Monica Rosa, López-Miranda, Santiago, Lucci, Paolo, Mahanta, Charu Lata, Malunga, Lovemore N., Mamede, Alexandra Mara Goulart Nunes, Manchali, Shivapriya, Martínez-Tomé, Magdalena, Martín-Pozuelo, Gala, Masisi, Kabo, Moreb, Nora, Murcia, M. Antonia, Murphy, Amy, Murthy, Kotamballi N. Chidambara, Nagraj, Geetha Shree, Nartea, Ancuta, Navarro-González, Inmaculada, Núñez-Delicado, Estrella, Ovando-Martínez, Maribel, O’Dwyer, Caron, Pacetti, Deborah, Pastoriza, Silvia, Patil, Bhimanagouda S., Pellicer, José Antonio, Pereira, Paula, Pérez-Alvarez, José Angel, Pérez-Burilo, Sergio, Periago, María Jesús, Picchi, Valentina, Pinela, José, Ramalho, Renata, Rashidinejad, Ali, Ravichandran, Kavitha, Rawal, Ranbeer S., Rawat, Sandeep, Río Celestino, Mercedes Del, Rodríguez-Arcos, Rocio, Rodríguez-López, María Isabel, Rojas, Ana María, Ross, Kelly A., Rufián-Henares, José A., Sassi, Chiraz Ben, Sharma, Shubham, Silva, Mafalda Alexandra, Siow, Yaw, Smetanska, Iryna, Soares, Antonio Gomes, Song, Yuwei, Srinivasan, Krishnapura, Sukketsiri, Wanida, Talbi, Wafa, Tlili, Imen, Tundis, Rosa, Tütem, Esma, Uzuner, Sibel, Vaillant, Fabrice, Vicente, Filipa, Viuda-Martos, Manuel, Wu, Han, and Xi, Wanpeng

Published
2020
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15. Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk

Author

Universitat Politècnica de València. Departamento de Química - Departament de Química, Generalitat Valenciana, Ministerio de Ciencia e Innovación, Ministerio de Educación y Ciencia, Tortajada Genaro, Luis Antonio, Santiago Felipe, Sara, Morais Ezquerro, Sergi Beñat, GABALDÓN, JOSE ANTONIO, Puchades Pla, Rosa, Maquieira Catala, Ángel, Universitat Politècnica de València. Departamento de Química - Departament de Química, Generalitat Valenciana, Ministerio de Ciencia e Innovación, Ministerio de Educación y Ciencia, Tortajada Genaro, Luis Antonio, Santiago Felipe, Sara, Morais Ezquerro, Sergi Beñat, GABALDÓN, JOSE ANTONIO, Puchades Pla, Rosa, and Maquieira Catala, Ángel

Abstract

The development of a DNA microarray method on a digital versatile disk (DVD) is described for the simultaneous detection of traces of hazelnut (Corylus avellana L.), peanut (Arachis hypogaea), and soybean (Glycine max) in foods. After DNA extraction, multiplex PCR was set up using S'-labeled specific primers for Cor a 1, Ar h 2, and Le genes, respectively. Digoxin-labeled PCR products were detected by hybridization with S'-biotinylated probes immobilized on a streptavidin-modified DVD surface. The reaction product attenuates the signal intensity of the laser that reached the DVD drive used as detector, correlating well with the amount of amplified sequence. Analytical performances showed a detection limit of 1 mu g/g and good assay reproducibility (RSD 8%), suitable for the simultaneous detection of the three targeted allergens. The developed methodology was tested with several commercially available foodstuffs, demonstrating its applicability. The results were in good agreement, in terms of sensitivity and reproducibility, with those obtained with ELISA, PCR-gel agarose electrophoresis, and RT-PCR.

Published
2012

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