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4 results on '"G4 induction"'

2. Ligand-Mediated G-Quadruplex Induction in a Double-Stranded DNA Context by Cyclic Imidazole/Lysine Polyamide

Author

Toshikazu Bando, Yue Li, Sefan Asamitsu, and Hiroshi Sugiyama

Subjects
0301 basic medicine, Stereochemistry, Context (language use), Ligands, 010402 general chemistry, G-quadruplex, 01 natural sciences, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Humans, Imidazole, A-DNA, Promoter Regions, Genetic, Molecular Biology, Protein secondary structure, cyclic polyamide, heterocycles, Ligand, Lysine, Organic Chemistry, Imidazoles, DNA, G-quadruplexes, 0104 chemical sciences, Nylons, 030104 developmental biology, chemistry, Cyclization, Molecular Probes, Molecular Medicine, molecular crowding condition, G4 induction, Molecular probe
Abstract

G-quadruplex (G4) DNA is often observed as a DNA secondary structure in guanine-rich sequences, and is thought to be relevant to pharmacological and biological events. Therefore, G4 ligands have attracted great attention as potential anticancer therapies or in molecular probe applications. Here, we designed cyclic imidazole/lysine polyamide (cIKP) as a new class of G4 ligand. It was readily synthesized without time-consuming column chromatography. cIKP selectively recognized particular G4 structures with low nanomolar affinity. Moreover, cIKP exhibited the ability to induce G4 formation of the promoter of G4-containing DNA in the context of stable double-stranded DNA (dsDNA) under molecular crowding conditions. This cIKP might be applicable as a molecular probe for the detection of potential G4-forming sequences in dsDNA.

Published
2016

3. Ligand-Mediated G-Quadruplex Induction in a Double-Stranded DNA Context by Cyclic Imidazole/Lysine Polyamide

Author

20345284, 50183843, Asamitsu, Sefan, Li, Yue, Bando, Toshikazu, Sugiyama, Hiroshi, 20345284, 50183843, Asamitsu, Sefan, Li, Yue, Bando, Toshikazu, and Sugiyama, Hiroshi

Abstract

G-quadruplex (G4) DNA is often observed as a DNA secondary structure in guanine-rich sequences, and is thought to be relevant to pharmacological and biological events. Therefore, G4 ligands have attracted great attention as potential anticancer therapies or in molecular probe applications. Here, we designed cyclic imidazole/lysine polyamide (cIKP) as a new class of G4 ligand. It was readily synthesized without time-consuming column chromatography. cIKP selectively recognized particular G4 structures with low nanomolar affinity. Moreover, cIKP exhibited the ability to induce G4 formation of the promoter of G4-containing DNA in the context of stable double-stranded DNA (dsDNA) under molecular crowding conditions. This cIKP might be applicable as a molecular probe for the detection of potential G4-forming sequences in dsDNA.

Published
2016

4. Ligand-Mediated G-Quadruplex Induction in a Double-Stranded DNA Context by Cyclic Imidazole/Lysine Polyamide.

Author

Asamitsu S, Li Y, Bando T, and Sugiyama H

Subjects
Cyclization, Humans, Imidazoles, Ligands, Lysine, Molecular Probes chemical synthesis, Nylons chemistry, Promoter Regions, Genetic, DNA chemistry, G-Quadruplexes, Nylons chemical synthesis
Abstract

G-quadruplex (G4) DNA is often observed as a DNA secondary structure in guanine-rich sequences, and is thought to be relevant to pharmacological and biological events. Therefore, G4 ligands have attracted great attention as potential anticancer therapies or in molecular probe applications. Here, we designed cyclic imidazole/lysine polyamide (cIKP) as a new class of G4 ligand. It was readily synthesized without time-consuming column chromatography. cIKP selectively recognized particular G4 structures with low nanomolar affinity. Moreover, cIKP exhibited the ability to induce G4 formation of the promoter of G4-containing DNA in the context of stable double-stranded DNA (dsDNA) under molecular crowding conditions. This cIKP might be applicable as a molecular probe for the detection of potential G4-forming sequences in dsDNA., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2016
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