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5 results on '"G. Z. Tong"'

1. Molecular cloning and physical mapping of bovine herpesvirus 4 strain DN 599 and comparison with two American field-isolates

Author

G. Z. Tong, David T. Shen, Dieter Burger, and J. R. Gorham

Subjects
Restriction Mapping, EcoRI, Deoxyribonuclease HindIII, Genome, Deoxyribonuclease EcoRI, Gene mapping, Tandem repeat, Virology, parasitic diseases, Animals, Cloning, Molecular, Herpesviridae, Southern blot, Genetics, Deoxyribonuclease BamHI, biology, General Medicine, Molecular biology, Molecular Weight, Restriction enzyme, Restriction site, DNA, Viral, biology.protein, Cattle, Restriction fragment length polymorphism
Abstract

Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The genome comprises a region of unique segment (114 kbp) and two flanking segments containing tandem repeats. The size of each repeat was approximately 2.35 kbp and each repeat contained one Eco RI site and two Bam HI sites. We also examined two recent American field-isolates of BHV-4 and compared the Eco RI maps of the two isolates with that of DN 599. We observed the following: (1) insertions or deletions of restriction sites at the periphery of the unique segment; (2) variation in the lengths of junction fragments; (3) variations in the lengths of hypermolar Eco RI fragments containing the repeats; and (4) the Eco RI map of one of the American field-isolates resembles the BHV-4 "Movar type" of Europe.

Published
1992

2. Isolation and molecular characterization of a porcine teschovirus 1 isolate from China

Author

L, Feng, H Y, Shi, S W, Liu, B P, Wu, J F, Chen, D B, Sun, Y E, Tong, M S, Fu, Y F, Wang, and G Z, Tong

Subjects
Swine Diseases, China, Picornaviridae Infections, Teschovirus, Reverse Transcriptase Polymerase Chain Reaction, Swine, Molecular Sequence Data, Brain, Sequence Homology, Sequence Analysis, DNA, Viral Proteins, Microscopy, Electron, Transmission, Animals, Domestic, Central Nervous System Viral Diseases, Animals, RNA, Viral, Phylogeny, Polyproteins
Abstract

Porcine teschovirus 1 (PTV-1) (Swine/CH/IMH/03) was isolated from piglets in a farm in Inner Mongolia Province, P.R. China. It was confirmed by electron microscopy, RT-PCR, and sequencing. Comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein showed that PTV Swine/CH/IMH/03 strain is PTV-1. The isolated virus has closest relationship with Talfan strain, they shared 98.9% and 99.5% homology of amino acids and nucleotides, respectively, in the ORF of polyprotein. To our knowledge, this is the first report about isolation and identification of a PTV in China.

Published
2007

3. Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus Emerged in China.

Author

Y.-J. Zhou, X.-F. Hao, Z.-J. Tian, G.-Z. Tong, D. Yoo, T.-Q. An, T. Zhou, G.-X. Li, H.-J. Qiu, T.-C. Wei, and X.-F. Yuan

Subjects
PORCINE reproductive & respiratory syndrome, SWINE diseases, VIRUS diseases in swine, COMMUNICABLE diseases in animals, VIRAL genomes, VIRAL proteins, GENETIC transcription, DIAGNOSIS
Abstract

A highly pathogenic pig disease emerged in China in 2006, which was characterized by prolonged high fever, red discoloration of the body, and blue ears associated with high mortality. Porcine reproductive and respiratory syndrome virus (PRRSV) was isolated as the single most prominent virus in the samples collected from affected pigs. The full-length genomic sequence of the virus revealed two distinct deletions in the non-structural protein 2 (NSP2) in comparison to all previously reported North American genotype PRRSV. Through extensive surveys in 14 different provinces, 56 additional PRRSV isolates were obtained from affected farms. All of the isolates were found to contain identical deletions in NSP2. To confirm the etiology, eight 60-day-old PRRSV-free pigs were divided into two groups and the test group was intranasally infected at a titer of 2 × 105.0 tissue culture infectious dose 50 per pig. The inoculated pigs all died at 7, 8, 12, 16, or 21 days post-inoculation with their clinical and pathological findings similar to those in the field. The viruses recovered from dead pigs were identical to the inoculated virus in NSP2 and GP5 genes. Our study shows that the recently emerged PRRSV in China is characterized by two discontiguous deletions in NSP2 and is the cause for the current epizootics in China. [ABSTRACT FROM AUTHOR]

Published
2008
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