1. Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
- Author
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Fengwei Jiang, N. M. A. Rahman, Wenyi Zhang, Shengming Sun, S B Jin, F F Jiang, Yongsheng Gong, Hongkun Bai, Yiwei Xiong, H T Fu, G. X. Liang, Yunyu Wu, and Hui Qiao
- Subjects
0301 basic medicine ,Male ,DNA, Complementary ,Biology ,Molecular cloning ,Arthropod Proteins ,03 medical and health sciences ,Complementary DNA ,Genetics ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Caenorhabditis elegans ,Molecular Biology ,Gene ,Phylogeny ,Cloning ,Regulation of gene expression ,Ovary ,Metamorphosis, Biological ,Gene Expression Regulation, Developmental ,General Medicine ,Androgen-Insensitivity Syndrome ,Sex Determination Processes ,Molecular biology ,Phenotype ,Fishery ,030104 developmental biology ,Prawn ,Female ,Macrobrachium nipponense ,Palaemonidae - Abstract
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. Real-time quantitative reverse polymerase chain reaction (RT-qPCR) showed that the MnFem1b gene was expressed in all investigated tissues, with the highest expression level found in the testes. The results revealed that the MnFem1b gene might play roles in aspects of development of the male prawn phenotype. The RT-qPCR also revealed that MnFem1b mRNA expression was significantly increased at 10 days after metamorphosis. The expression levels in all investigated tissues showed a certain degree of sexually dimorphism, the expression levels in males were significantly higher than those in females (P < 0.05). Notably, the highest expression of MnFem1b was found in the testes. The expression of MnFem1b in different tissues indicates that it plays multiple biological functions in M. nipponense.
- Published
- 2016